Weihua Guo

Shandong University | SDU · School of Life Science

Groundwater arsenic (As), contamination is a significant issue worldwide including China and Pakistan, particularly in canal command areas. In this study, 131 groundwater samples were collected, and three machine learning models [Random Forest (RF), Logistic Regression (LR), and Artificial Neural Network (ANN)] were employed to predict As concentra...

Invasive plants can profoundly disrupt native biodiversity, yet the genetic mechanisms underpinning their success remain poorly understood. To date, genomic studies have been conducted on only a limited number of invasive species, and no single-cell level studies have been applied. This research investigates the genetic drivers behind the invasive...

In the last decade, microplastics (MPs) have become a significant environmental pollutant with potential negative effects on aquatic biodiversity and ecosystems. This mesocosm study examined the effect of MPs on the growth and physiology of two common aquatic macrophytes (Myriophyllum spicatum and Phragmites australis), focusing on changes in bioma...

Background Soil salinization is a growing global concern in many ecosystems. Although ectomycorrhizal fungi have been shown to alleviate the effects of salinity in some tree species, uncertainties persist concerning their effectiveness when plants are exposed to different salinity levels that are commonly present in salt-affected soils. Objective T...

Polyploidization plays an important role in plant speciation and adaptation. To address the role of polyploidization in grass diversification, we studied Phragmites australis , an invasive species with intraspecific variation in chromosome numbers ranging from 2n = 36 to 144. We utilized a combined analysis of ploidy estimation, phylogeny, populati...

Coal gangue hill, a significant anthropogenic interference, can cause various forms of land degradation. The promoting effect of coal gangue on soil qualities has also been discovered. However, few studies investigated the soil properties and microbiome of prolonged gangue hills. Here, we investigated soil microbial communities and chemical propert...

Biological invasions pose a significant threat to ecosystems, disrupting local biodiversity and ecosystem functions. The genomic underpinnings of invasiveness, however, are still largely unknown, making it difficult to predict and manage invasive species effectively. The common reed (Phragmites australis) is a dominant grass species in wetland ecos...

Hard tissue engineering scaffolds especially 3D printed scaffolds were considered an excellent strategy for craniomaxillofacial hard tissue regeneration, involving crania and facial bones and teeth. Porcine treated dentin matrix (pTDM) as xenogeneic extracellular matrix has the potential to promote the stem cell differentiation and mineralization a...

The decellularized matrix has a great potential for tissue remodeling and regeneration; however, decellularization could induce host immune rejection due to incomplete cell removal or detergent residues, thereby posing significant challenges for its clinical application. Therefore, the selection of an appropriate detergent concentration, further op...

Invasive plants can interact with soil microbes to enhance their own performance. Such interactive effects may persist and later affect plant performance and their population dynamics. Such ‘invasive soil legacy’ is the specific plant–soil feedback that can affect future invasions, while it is not clear how nitrogen deposition and interspecific com...

Coastal wetland ecosystems are increasingly threatened by escalating salinity levels, subjecting plants to salinity stress coupled with interactions in community. Abiotic factors can disrupt the balance between competition and facilitation among plant species. Investigating the effects of different neighboring species and trait plasticity could ext...

Biodiversity is considered important to the mitigation of global change impacts on ecosystem multifunctionality in terrestrial ecosystems. However, potential mechanisms through which biodiversity maintains ecosystem multifunctionality under global change remain unclear. We grew 132 plant communities with two levels of plant diversity, crossed with...

In this study, we conducted an in situ organic fertilizer application experiment in an Suaeda salsa salt marsh wetland. We evaluated the growth conditions of S. salsa, soil physicochemical properties, and composition of the soil bacterial community one year after fertilizer input. Additionally, we elaborated on the mechanism of bacterial community...

Aims Nitrogen (N) supply and precipitation pattern (amount and frequency) both affect plant growth. However, N deposition is increasing and precipitation regimes are changing in the context of global change. An experiment was conducted to access how the growth of Robinia pseudoacacia, a widely distributed and cultivated N2-fixing alien species, is...

Within‐species genetic diversity is shaped by multiple evolutionary forces within the confines of geography, and has cascading effects on the biodiversity of other taxa and levels. Invasive species are often initially limited in genetic diversity but still respond rapidly to their new range, possibly through ‘pre‐adapted' genotypes or multiple sour...

Background CDC42 is a member of Rho GTPase family, acting as a molecular switch to regulate cytoskeleton organization and junction maturation of epithelium in organ development. Tooth root pattern is a highly complicated and dynamic process that dependens on interaction of epithelium and mesenchyme. However, there is a lack of understanding of the...

Introduction Fire and nitrogen (N) deposition each impact biodiversity and ecosystem productivity. However, the effect of N deposition on ecosystem recovery after fire is still far from understood, especially in coastal wetlands. Methods We selected a typical coastal shrubland to simulate three N deposition levels (0, 10, and 20 g N m⁻² year⁻¹) un...

Recent advances in three‐dimensional (3D) printing offer a prospective avenue for producing transplantable human tissues with complex geometries; however, the appropriate 3D‐printed scaffolds possessing the biological compatibility for tooth regeneration remain unidentified. This study proposes a personalized scaffold of multiple bioactivities, inc...

Biodiversity is hypothesized to mitigate global change impacts on ecosystem multifunctionality in terrestrial ecosystems. However, potential mechanisms through which biodiversity maintains ecosystem multifunctionality under global change remain unclear. We grew 132 plant communities with two levels of plant diversity, crossed with treatments based...

Background Oral tongue cancer is an extremely malignant tumor with high rate of lymphatic metastasis. Little is known about its invasion and metastasis mechanisms so far. Methods To clarify the main role of CCL2 in tongue cancer progression, we performed Transwell migration assay to confirm the effects of different concentrations of CCL2 on the mi...

Xenogenic organ transplantation has been considered the most promising strategy in providing possible substitutes with the physiological function of the failing organs as well as solving the problem of insufficient donor sources. However, the xenograft, suffered from immune rejection and ischemia-reperfusion injury (IRI), causes massive reactive ox...

Background: Oral tongue squamous cell carcinoma tends to metastasize to cervical lymphatic nodes early which leads to a 50% drop of survival rate. CXCL1 could be secreted by LNMTca8113 cell induced lymphatic endothelial cells and promoted LNMTca8113 cell migration. The current study aimed to further explore the effect of CXCL1 on the proliferation...

The unique structure of the periodontium, including the alveolar bone, cementum, and periodontal ligament (PDL), presents difficulties for the regeneration of its intricate organization. Irreversible structural breakdown of the periodontium increases the risk of tooth loosening and loss. Although the current therapies can restore the periodontal ha...

The treatment of adolescent skeletal open-bite malocclusion with severe molar-incisor hypomineralization (MIH) remains challenging. Though conducive to open-bite treatment and endodontic management, early molar extraction may trigger a series of negative impacts on occlusion and stomatognathic development. In addition, molars' crown restoration was...

Cell polarity is essential for ameloblast differentiation and enamel formation. Smurf1 can mediate cell polarization through ubiquitination degradation of specific substrates. But it remains unclear whether Smurf1 could regulate ameloblast polarity and the underlying mechanism. Here, immuno-fluorescence staining and RT-qPCR were applied to detect t...

Key message Defoliation significantly affected biomass allocation of Robinia pseudoacacia L. and Sophora japonica L., but leaf physiology readjusted to control levels at the end of the experiment. Considering carbon or sink limitation and relative height growth rate, defoliated R. pseudoacacia grew faster than S. japonica under well-watered conditi...

With the enhancement of carbon sink capacity and the intensification of biodiversity change in coastal wetlands, previous studies have well studied the relationship between species diversity and litter decomposition, a vital process of carbon cycling. However, previous studies ignored the importance of genetic diversity, which restricted our compre...

Reactive oxygen stress (ROS) is generally accepted as a signal transducer for coordinating the growth and differentiation of tissues and organs in the oral and maxillofacial region. Although ROS has been confirmed to affect the development of enamel, it is not yet known that the specific mechanism of ROS accumulation induced enamel defects. Given t...

Background and aims Cotyledons are important nutrient sources for seedling establishment in seed plants, however, the acorns are often consumed by rodents and/or insects. This study aimed to examine the compensation or enhancement effect of soil nutrient for cotyledon damage on seedlings of Quercus acutissima under different light conditions. Meth...

The presence of large amounts of antibiotic resistance genes (ARGs) in livestock manures poses an impending, tough safety risk to ecosystems. To investigate more comprehensively the mechanisms of ARGs removal from industrial-scale composting of livestock manure based on biochar addition, we tracked the dynamics of bacterial community and ARGs at va...

Soil salinization is one of the most severe environmental problems restricting biodiversity maintenance and ecosystem functioning in a coastal wetland. Recent studies have well documented how salinization affects soil microbial communities along vegetation succession of coastal wetlands. However, the salinity effect is rarely assessed in the contex...

Among all the adult stem cells, odontogenic stem cells inherit the characterization of neurogenic potential of their precursor ones–the cranial crest cells. Dental follicle cells (DFCs), one of the special kind of odontogenic stem cells, are raising interest in applying to regenerative medicine for they possess multi-differentiation potential, rela...

There are different environmental pressures in coastal and inland wetlands resulting in phenotypic variation among plant populations, which might be related to epigenetic modifications. Phragmites australis is a widespread plant in coastal and inland wetlands, and the phenotype of the population is selected by salinity, but the reasons for the popu...

Soil salinization has been one of the main causes of ecosystem degradation in many estuarine wetlands under global climate changes, but it remains unclear how salinization shifts the phenotypic variability and genetic diversity of the foundation plant species in estuarine wetlands. To reveal the effects of salinization on natural populations of fou...

Background: Though researchers have been exploring therapeutic strategies of treating serious periodontal tissue loss including the application of stem cells, tissue regeneration of the periodontal complex involving cementum, periodontium and alveolar bone has hardly been achieved. Aiming at tackling the problem of severely damaged periodontal com...

Stem cell/scaffold-based tissue engineering technology is expected to regenerate tooth root, thus replace dental implant. Although numerous patterns of biological tooth root have been constructed, most of them failed for the reasons involved the type of selected stem cells, scaffold materials, and the recombination strategy of cell-scaffold complex...

Aims Invasive plants often succeed despite attack by generalist herbivores in the introduced range. Whether invasive plants tolerate herbivory by adjusting functional traits, modulating the rhizosphere bacterial community, or both, and how root exudates of native plants influence these tolerance mechanisms, is yet to be tested. Methods We examined...

Soil microorganisms play vital roles in regulating biogeochemical processes. The composition and function of soil microbial community have been well studied, but little is known about the responses of bacterial and fungal communities to different habitats of the same plant, especially the inter-kingdom co-occurrence pattern including bacteria and f...

Three-dimensional (3D) bioprinting is an emerging method for tissue regeneration. However, promoting the epithelial-mesenchymal interaction (EMI), while maintaining the characteristics of epithelial cells has always been a challenge in tissue engineering. Since EMI acts as a critical factor in bone regeneration, this study aims to promote EMI by re...

Climate change causes the global redistribution of precipitation, yet little is known about the effects of the changes in precipitation intensity and frequency on the seedlings of wood trees in warm temperate forests. In this study, we focused on the effects of variability in both the intensity and frequency of water supply on the physiological tra...

Objective xenogenic organ transplantation has been considered the most promising strategy in providing possible subtitutes with physiological function of the failing organs as well as solving the problem of insufficient donor sources. However, the xenograft, suffered from immune rejection and ischemia-reperfusion injury (IRI), causes massive ROS ex...

Aims Nitrogen (N) supply and precipitation pattern (amount and frequency) both affect plant growth. However, N deposition is increasing and precipitation regimes are changing in the context of global change. An experiment was conducted to determine the effect of precipitation pattern and N supplies on the growth of a widely distributed and planted...

A new species Vanakripa chinensis found on decaying branches of an unidentified tree is described and illustrated. It is characterized by sporodochial conidiomata composed of macronematous, cylindrical conidiophores that are sometimes reduced to conidiogenous cells and obovoid, unicellular, brown, smooth conidia with a black spot near the middle. N...

Aims Soil legacies mediate interactions between native and introduced plants, contributing to both invasion and biotic resistance to invasion. Given that nitrogen deposition can promote allelochemical release, reduce the benefits of soil microbes, and affect trait plasticity, nitrogen deposition likely alters soil legacies as well. However, it is n...

Polyploidization is a common event in plant evolution, and it plays an important role in plant speciation and adaptation. To address the role of polyploidization in grass diversification, we studied Phragmites australis, a species with intraspecific variation of chromosome numbers ranging from 2n=36 to 144. A combined analysis of genome structure,...

Soil salinity diminishes the dominance of species and affects their distribution. Phragmites australis is a dominant ecosystem engineer with broad distribution, high intraspecific variation and great socio-economic importance. Coastal ecosystems inhabited by P. australis are threatened by salinization. Here, we investigated salinity tolerance of fr...

Phragmites australis is a cosmopolitan plant species with high intraspecific diversity and phenotypic plasticity. Due to its variability and large ecological niche breadth, subgroups of P. australis have become invasive in North America, and this invasion has been recognized late. While this cryptic invasion on the American continent has received m...

Xenogenic extracellular matrix (xECM)-based organ transplantation will be a promising approach to address the problem of donor shortage for allotransplantation, which has achieved great success in organ regeneration. However, current approaches to utilize xECM-based organ have limited capacity to yield the host a biofriendly microenvironment for lo...

Phragmites australis is the dominant species in the Yellow River Delta and plays an important role in wetland ecosystems. In order to evaluate the relationship between phenotypic variation and environmental factors, explore how functional traits respond to changes in electrical conductivity and soil water content, and reveal the ecological strategi...

For a comprehensive insight into the potential mechanism of the removal of antibiotic resistance genes (ARGs) removal induced by initial substrates during composting, we tracked the dynamics of physicochemical properties, bacterial community composition, fungal community composition, the relative abundance of ARGs and mobile genetic genes (MGEs) du...

Invasions are a major threat to the biodiversity of native ecosystems. European lineages of Phragmites australis are invasive in North America, where they compete with the native subspecies and have expanded to regions previously not known to inhabit P. australis. Nonetheless, there is relatively little attention paid to the risk assessment and pre...

Polyploidization in plants is thought to have occurred as coping mechanism with environmental stresses. Polyploidization-driven adaptation is often achieved through interplay of gene networks involved in differentially expressed genes, which triggers the plant to evolve special phenotypic traits for survival. Phragmites australis is a cosmopolitan...

Successful regenerative medicine strategies of xenogeneic extracellular matrix need a synergistic balance among inflammation, fibrosis, and remodeling process. Adaptive macrophage subsets have been identified to modulate inflammation and orchestrate the repair of neighboring parenchymal tissues. This study fabricated PPARγ-primed CD68⁺CD206⁺ M2 phe...

Background The low survival rate or dysfunction of extracellular matrix (ECM)-based engineered organs caused by the adverse effects of unfavourable local microenvironments on seed cell viability and stemness, especially the effects of excessive reactive oxygen species (ROS), prompted us to examine the importance of controlling oxidative damage for...

Phragmites australis is the dominant species in the Yellow River Delta and plays an important role in wetland ecosystems. Ecological responses of the P. australis community to soil properties were investigated in 96 areas along the coastal-inland regions in the Yellow River Delta of China. The aim was to evaluate the relationship between phenotypic...

Purpose Universal germline testing in patients with colorectal cancer (CRC) with a multigene panel can detect various hereditary cancer syndromes. This study was performed to understand how to choose a testing panel and whether the result would affect clinical management. Methods We prospectively enrolled 486 eligible patients with CRC, including...

The status of reactive oxygen species (ROS) correlates closely with the normal development of the oral and maxillofacial tissues. Oxidative stress caused by ROS accumulation not only affects the development of enamel and dentin but also causes pathological changes in periodontal tissues (periodontal ligament and alveolar bone) that surround the roo...

As one of the youngest coastal wetland ecosystems in China, the Yellow River Delta has a gradual succession from bare land to grassland. Six typical communities were selected for the present study of the patterns and dynamics of soil microbial community under the gradual transitional succession. In which soil physicochemical properties such as soil...

Rivers favour gene flow by hydrochory and habitat connectivity, and bring heterogeneous selection pressures on genotypes. River regulations (e.g. dams) could influence the hydrologic conditions and then change the effects of rivers on genetic diversity of riverine plants. In Ningxia Plain of China, we investigated the intraspecific variation of Phr...

Biodiversity–biomass relationships have been debated for decades and remain subject to controversy. Understanding the relationship between biodiversity and biomass will be crucial for soil and water conservation of the whole basin of the Yellow River. The positive effects of biodiversity on aboveground biomass are altered via two fundamental mechan...

Background The effectiveness of anterior crossbite treatment in preschool-aged children depends on the treatment design and patient compliance. Common early treatment appliances with steel wires and acrylic resin can bring about numerous problems, such as toothache, sore gums and mucous membrane injury. The aim of this study was to propose a new cl...