Wanwan Chen

• Student at University of Cincinnati

Objective This study aimed to examine the temporal relationship between body mass index (BMI) and uric acid (UA), and their joint effect on blood pressure (BP) in children and adults. Methods The longitudinal cohorts for temporal relationship analyses consisted of 564 and 911 subjects examined twice 5–14 years apart from childhood to adulthood. Th...

Sulphate‐reducing bacteria (SRB) are widespread in human guts, yet their expansion has been linked to colonic diseases. We report the isolation, sequencing, and physiological characterisation of strain QI0027T, a novel SRB species belonging to the class Desulfovibrionia. Metagenomic sequencing of stool samples from 45 Chinese individuals, and compa...

The NA60 experiment has studied low-mass muon pair production in proton-nucleus collisions with a system of Be, Cu, In, W, Pb and U targets, using a 400 GeV proton beam at the CERN SPS. The transverse momentum spectra of the $\rho/\omega$ and $\phi$ mesons are measured in the full $p_\mathrm{T}$ range accessible, from $p_\mathrm{T} = 0$ up to 2 GeV...

Organic anode materials have attracted considerable interest owing to their high tunability by adopting various active functional groups. However, the interaction mechanisms between the alkali metals and the active functional groups in host materials have been rarely studied systematically. Here, a widely used organic semiconductor of perylene-3,4,...

Lactobacillus is the dominant genus during fruit and vegetable juices (FVFs) fermentation, which are the key factors for taste and flavor. This study was performed to investigate the effects of different Lactobacillus spp. on profile of volatile flavor compounds and nonvolatile taste compounds in FVFs fermentation. A total of 14 compounds were iden...

The NA60 experiment has studied low-mass muon pair production in proton–nucleus collisions with a system of Be, Cu, In, W, Pb and U targets, using a 400 GeV proton beam at the CERN SPS. The transverse momentum spectra of the \(\rho /\omega \) and \(\phi \) mesons are measured in the full \(p_{\mathrm {T}}\) range accessible, from \(p_{\mathrm {T}}=...

Thioesterases (TEs) play an essential role in the metabolism of fatty acids (FAs). To explore the role of TEs in mediating intracellular lipid metabolism in the oleaginous fungus Mortierella alpina, the acyl-CoA thioesterase ACOT8I was overexpressed. The contents of total fatty acids (TFAs) were the same in the recombinant strains as in the wild-ty...

Background Video display terminal (VDT)-associated dry eye (DE) patients are the rising group worldwide, and moisture goggles are the preferable treatment since they are capable of improving tear film stability and DE discomfort. The current study aims to evaluate the short-term efficacy and safety of the developed warming moist chamber goggles (WM...

[This corrects the article on p. 2104 in vol. 8, PMID: 29312376.].

Background: Economic literature shows that a child's future earnings are predictably influenced by parental income, providing an index of "socioeconomic mobility," or the ability of a person to move towards a higher socioeconomic status from childhood to adulthood. We adapted this economic paradigm to examine cardiovascular risk mobility (CRM), or...

Structures determine properties of materials and controllable phase transitions are, therefore, highly desirable for exploring exotic physics and fabricating devices. We report a direct observation of a controllable semiconductor-metal phase transition in bilayer tungsten diselenide (WSe2) with potassium (K) surface functionalization. Through the i...

Regarding the shortcomings of the cross-coupling control structure during the start-up of a multi-motor with load—namely, a large synchronization error and a long start-up time—this paper proposes a fuzzy self-adjusting cross-coupling control structure. This structure combines a fuzzy self-adjusting filter and an advanced synchronization compensato...

In the industrial oleaginous fungus Mortierella alpina, the arachidonic acid (AA; C20:4; ω-6) fraction can reach 50% of the total fatty acids (TFAs) in vivo. However, the eicosapentaenoic acid (EPA; C20:5; ω-3) fraction is less than 3% when this fungus is cultivated at a low temperature (12°C). Omega-3 fatty acid desaturase is a key enzyme in ω-3 l...

For the industrial production of probiotics powder, various sugars have been used as cryoprotectants to preserve probiotics during freeze-drying. Some of these sugars can be metabolized by Lactobacillus with the production of acids during the mix. In this study, we investigated the effect of acids on ATPase, β-galactosidase, lactate dehydrogenase (...

Aims: This study evaluated the antibiotic-induced changes in microbial ecology, intestinal dysbiosis and low-grade inflammation; and the combined effect of four different Lactobacillus species on recovery of microbiota composition and improvement of gut barrier function in mice. Methods and results: Administration of the antibiotic ampicillin fo...

Thermally stable Au single-atoms supported by monolayered CuO grown at Cu(110) have been successfully prepared. The charge transfer from the CuO support to single Au atoms is confirmed to play a key role in tuning the activity for CO oxidation. Initially, the negatively charged Au single-atom is active for CO oxidation with its adjacent lattice O a...

Polyunsaturated fatty acids (PUFAs) are essential lipids for cell function, normal growth, and development, serving as key structural components of biological membranes and modulating critical signal transduction events. Omega-3 (n-3) long chain PUFAs (LC-PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been shown to p...

In Li–CO2 battery, due to the highly insulating nature of the discharge product of Li2CO3, the battery needs to be charged at a high charge overpotential, leading to severe cathode and electrolyte instability and hence poor battery cycle performance. Developing efficient cathode catalysts to effectively reduce the charge overpotential represents on...

Aspirin as an antitumor drug has been studied in various malignancies with regards to its effects on apoptosis, proliferation, metastasis and senescence of tumor cells. However, the clinical application is limited by its side effects. Nutlin‑3 is a novel antitumor compound, which has not been clinically approved. The present study investigated the...

Oxidative stress and its correlation with degenerative diseases have been attracting wide attention. In the present study, Lactobacillus plantarum (L. plantarum) CCFM10 and RS15-3 were examined for their abilities to resist oxidative stress in d-galactose (d-gal)-exposed mice. Both strains significantly reversed the changes of hepatic total antioxi...

Tea leaf color is not only important from an aesthetics standpoint but is also related to tea quality. To investigate the molecular mechanisms that determine tea leaf color, we examined Camellia sinensis cv. ‘Anji Baicha’ (an albino tea cultivar) by tandem mass tag isobaric labeling to generate a high-resolution proteome and acetyl-proteome atlas o...

Our previous study confirmed the protective potential of Lactobacillus plantarum (L. plantarum) strains in alleviation of cadmium (Cd) toxicity in vivo and demonstrated that the observed protection largely depended on the tolerance of the strains to Cd-induced stress. It was also observed that there were significant intra-species differences in Cd...

To explore novel molecular mechanisms underlying obesity, we applied a systems genetics framework to integrate risk genetic loci from the largest body mass index (BMI) genome-wide association studies (GWAS) meta-analysis with mRNA and microRNA profiling in adipose tissue from 200 subjects. One module was identified to be most significantly associat...

Tear film hyperosmolarity and anterior ocular inflammation are two clinical signs that may be indicative of dry eye disease (DED). This condition can cause pathological and functional changes to the anterior ocular surface tissues. A contributing factor may be dysfunctional aquaporin 5 (AQP5) water channels as they are the AQP subtype that expresse...

Patients with type 2 diabetes (T2D) have decreased butyrate-producing bacteria. We hypothesized that supplementation with butyrate-producing bacteria may exert beneficial effects on T2D. The current study investigated the effects of well-characterized butyrate-producing bacteria Clostridium butyricum CGMCC0313.1 (CB0313.1) on hyperglycemia and asso...

This study aims to develop a cross-linked decellularized porcine corneal graft (cDPC) as a substitute for lamellar donor corneas and to evaluate the feasibility of using cDPC to treat fungal keratitis. The cDPC was prepared by decellularization, chemical crosslinking and γ-ray irradiation. Transparency, effectiveness of decellularization and biomec...

This review evaluates the evidence from animal studies for the protective effects of probiotics administration on d-galactose-induced oxidative stress. Of 192 articles identified from Web of Science, Embase and PubMed, 8 articles were included in the meta-analysis. The studies included 280 mice in total. Estimates of plasma superoxide dismutase (SO...

This study evaluated the quenching and extraction procedures using different solvents at different temperatures for Lactobacillus plantarum ZS2058. Eight quenching methods that used readily available organic solvents (methanol and ethanol) or cryoprotectants (glycerol and dimethyl sulfoxide) were evaluated quantitatively, and their effects on cell...

Objective: To examine the relationship between reproductive history, pregnancy complications, and later physical function. Study design: The Bogalusa Heart Study is a long-running study of cardiovascular health in a semirural community. 761 women were interviewed about their pregnancy history and complications, and underwent tests of physical fu...

The next-generation high-throughput sequencing techniques have introduced a new way to assess the gut’s microbial diversity on the basis of 16S rRNA gene-based microbiota analysis. However, the precise appraisal of the biodiversity of Bifidobacterium species within the gut remains a challenging task because of the limited resolving power of the 16S...

Monolayer two-dimensional (2D) transition metal dichalcogenides (TMDs) show interesting optical and electrical properties because of their direct bandgap. However, the low absorption of atomically thin TMDs limits their applications. Here, we reported enhanced absorption and optoelectronic properties of monolayer molybdenum disulfide (MoS2) by usin...

Functional genomics of filamentous fungi has gradually uncovered gene information for constructing ‘cell factories’ and controlling pathogens. Available gene manipulation methods of filamentous fungi include random integration methods, gene targeting technology, gene editing with artificial nucleases and RNA technology. This review describes random...

The aim of this study was to evaluate the effects of different kinds of oligosaccharides on cecal microbiota. These oligosaccharides were a fructo-oligosaccharide (FOS) formulation consisting of 95% FOS (FOS95), a galacto-oligosaccharide (GOS) formulation consisting of 90% GOS (GOS90), and an isomalto-oligosaccharide (IMO) formulation consisting of...

Purpose To assess the performance of Support Vector Machines (SVM) classification to stratify the Gleason Score (GS) of prostate cancer (PCa) in the central gland (CG) based on image features across multiparametric magnetic resonance imaging (mpMRI). Materials and methods This retrospective study was approved by the institutional review board, and...

Variation in temperature causes changes in the composition of the casein micelles. Milk samples with 10, 20, 30, 40, and 50% reduction of total β-CN from casein micelles were prepared and analysed. The micelle structure was maintained with minor changes in z-average diameters, zeta potentials, and micellar calcium. During acid gelation decreases in...

Background/aims: This study aims to identify whether Urothelial Cancer Associated 1 (UCA1) regulates mitochondrial metabolic reprogramming in bladder cancer, and to explore how UCA1 participates in mitochondrial metabolism by the UCA1/miR-195/ARL2 signaling pathway; these findings may be aid in the development of tumor diagnostic and therapeutic s...

Background: The nutritional status and systemic inflammation are thought to be associated with outcome in multiple types of cancer. The objective of this study was to determine the prognostic value of pretreatment albumin and fibrinogen combined prognostic grade (AFPG) in prostate cancer (PCa). Methods: 462 prostate cancer patients who had undergon...

Recent evidence indicates that indigenous Clostridium species induce colonic regulatory T cells (Tregs), and gut lymphocytes are able to migrate to pancreatic islets in an inflammatory environment. Thus, we speculate that supplementation with the well-characterized probiotics Clostridium butyricum CGMCC0313.1 (CB0313.1) may induce pancreatic Tregs...

Insulitis score for non-obese diabetic (NOD)-CB and NOD control mice. Percentage of islets with a given score at 13 weeks of age in NOD-CB (n = 23) and NOD control (n = 23) mice. (A) 1 = white, no infiltration; (B) 2 = light gray, few mononuclear cells infiltrated; (C) 3 = gray, peri-insulitis; (D) 4 = dark gray, <50% islet infiltration; (E) 5 = bl...

(A) Relative clostridial cluster IV abundance; (B) Relative clostridial cluster XIVa abundance. Data are mean ± SEM, (n = 3–5 mice per group). *, **, *** p < 0.05, p < 0.01, p < 0.001 vs NOD control mice by t-test.

Information of antibody for FACS.

Methods of gating for FACS.

Percent α4β7+ cells in PLN Tregs. Data are mean ± SEM (n = 5–8 mice per group). *, **, *** p < 0.05, p < 0.01, p < 0.001 vs NOD control mice by t-test.

Percent CD11c+ cells in splenic CD45+ cells. *, **, *** p < 0.05, p < 0.01, p < 0.001 vs NOD control mice by t-test.

Percent CD4+Foxp3+ cells in pancreas as indicated at 45 weeks of age.

Percent CD4+ cells in MLN and PLN. (A) Percent CD4+ cells in MLN as indicated at 6, 9, and 13 weeks of age in the previous experiments using the antibody of CD4 (eBioscience, FITC). (B) Percent CD4+ cells in MLN e as indicated at 6 weeks of age in the additional experiments using the new antibody of CD4 (Miltenyi, PE-vio770). (C) Percent CD4+ cells...

Primer sequences used in this study for microbial abundance.

Information of antibody for FACS for the experiment of regulatory T cells migration.

Information of antibody for FACS for the experiments of CD45+CD11c+.

The synthesis of free-standing two-dimensional titania (2-D TiO2) with a reduced band gap presents complex chal-lenges to synthetic chemists. Here, we report a free-standing 2-D TiO2 sheet synthesized via a one-step solvothermal methodology, with a measured optical onset at ~ 1.84 eV. Using first principles calculations in combination with experime...

As a natural inhabitant of gastrointestinal tract, Lactobacillus plantarum is well known for its health-promoting effects, especially in heavy metal-removal function. The response mechanism integrated physiology and proteomics of Lactobacillus plantarum under excessive manganese stress was systematically performed. More extracellular polymeric subs...

This study aimed to retrospectively investigate the factors related to pediatric obstructive sleep apnea–hypopnea syndrome (OSAHS) with attention deficit hyperactivity disorder (ADHD) in children younger than 6 years and those older than 6 years. A total of 437 children who were hospitalized due to OSAHS between January 2014 and December 2014 were...

Sodium accumulates in the interstitium and promotes inflammation through poorly defined mechanisms. We describe a pathway by which sodium enters dendritic cells (DCs) through amiloride-sensitive channels including the alpha and gamma subunits of the epithelial sodium channel and the sodium hydrogen exchanger 1. This leads to calcium influx via the...

Correction for ‘Recent advances in understanding of the mechanism and control of Li 2 O 2 formation in aprotic Li–O 2 batteries’ by Zhiyang Lyu et al. , Chem. Soc. Rev. , 2017, DOI: 10.1039/c7cs00255f.

Both leukocyte telomere length (LTL) and DNA methylation age are strongly associated with chronological age. One measure of DNA methylation age─ the extrinsic epigenetic age acceleration (EEAA)─ is highly predictive of all-cause mortality. We examined the relation between LTL and EEAA. LTL was measured by Southern blots and leukocyte DNA methylatio...

In order to increase fundamental voltage transfer ratio for ultra sparse matrix converter (USMC), a novel multi-mode space vector overmodulation strategy is proposed. Firstly, the relationship among voltage transfer ratio (VTR), the output voltage harmonic distortion rate under three inverter modulation methods and the input current harmonic distor...

Schottky barriers greatly influence the performance of optoelectronic devices. Schottky barriers can be reduced by harnessing the polymorphism of 2D metal transition dichalcogenides, since both semiconducting and metallic phases exist. However, high energy, high temperature or chemicals are normally required for phase transformation, or the process...

Correction for ‘ Lactobacillus casei CCFM419 attenuates type 2 diabetes via a gut microbiota dependent mechanism’ by Gang Wang et al. , Food Funct. , 2017, DOI: 10.1039/c7fo00593 h.

We investigated the protection offered by the probiotic Lactobacillus plantarum CCFM639 against waterborne Al exposure in tilapia. Fish were allocated to control, CCFM639-only, Al-only or Al plus CCFM639 groups. The fish were exposed to 2.73 mg/L Al ions for 4 weeks. The probiotic was incorporated into the fish diet at 10⁸ CFU/g and provided twice...

Metallothermal reaction growth of In2O3 nanocrystals via chemical vapor deposition technique gave rise to different morphologies, ranging from octahedron to triamond triangular frustum and their corresponding truncated variants. The formation of these polyhedral crystalline structures was triggered by high local temperature and supersaturation rati...

s Conjugated linoleic acid (CLA) has been shown to exert various potential physiological properties including anti-carcinogenic, anti-obesity, anti-cardiovascular and anti-diabetic activities, and consequently has been considered as a promising food supplement. Bacterial biosynthesis of CLA is an attractive approach for commercial production due to...

Chemical vapor deposition (CVD) is the most promising approach for producing low-cost, high-quality, and large area graphene. Revealing the graphene growth mechanism at the atomic-scale is of great importance for realizing single crystal graphene (SCG) over wafer scale. Density functional theoretical (DFT) calculations are playing an increasingly i...

Mobile element insertions (MEIs) represent ~25% of all structural variants in human genomes. Moreover, when they disrupt genes, MEIs can influence human traits and diseases. Therefore, MEIs should be fully discovered along with other forms of genetic variation in whole genome sequencing (WGS) projects involving population genetics, human diseases,...

Developing catalysts to improve excitonic charge-carrier transfer and separation properties is critical for solar energy conversion through photochemical catalysis. Layer staking of two-dimensional materials has opened up opportunities to engineer heteromaterials for strong interlayer excitonic transition. However, scalable fabrication of heteromat...

Background To overcome the hostile hypoxic microenvironment of solid tumors, tumor cells secrete a large number of non-coding RNA-containing exosomes that facilitate tumor development and metastasis. However, the precise mechanisms of tumor cell-derived exosomes during hypoxia are unknown. Here, we aim to clarify whether hypoxia affects tumor growt...

CpG oligodeoxynucleotides (ODNs) activate immune system and induce Th 1 responses by stimulation of Toll-like receptor 9 (TLR9). Thus, CpG ODNs become immunotherapeutics against various diseases including cancers, allergies, and infection. However, applications of CpG ODNs are largely limited because of their easy degradation by DNase as well as in...

Previous studies have suggested that dietary alpha-linolenic acid (ALA) increases the levels of omega-3 long-chain polyunsaturated fatty acids (ω-3 LC-PUFAs) in vivo, but the conversion procedure and the genes involved remain poorly understood. In the present work, we designed diets containing various concentrations of ALA and eicosapentaenoic acid...

Aim: To assess the mechanism for CLA production in Lactobacillus plantarum ZS2058. Methods and results: Conjugated linoleic acid (CLA) has attracted great interests for decades due to its health-associated benefits including anti-cancer, anti-atherogenic, anti-obesity, and modulation of the immune system. A number of microbial CLA producers were...

Constipation is one of the most common gastrointestinal complaints worldwide. The aim of this study was to determine whether edible Bifidobacteria (B. longum, B. infantis, B. animalis, B. bifidum, B. adolescentis or B. breve) exerts interspecies differences in alleviating constipation induced by loperamide in BALB/C mice and to analyse the main rea...

Metastasis frequently occurs in advanced ovarian cancer, which not only leads to substantial mortality but also becomes a major challenge to effective treatment. Epithelial-mesenchymal transition (EMT) is a key mechanism facilitating cancer metastasis. Targeting the EMT process with more efficacious and less toxic agents to prevent metastasis is of...

The superconducting quantum interference device (SQUID) amplifier is widely used in the field of weak signal detection for its low input impedance, low noise, and low power consumption. In this paper, the SQUIDs with identical junctions and the series SQUIDs with different junctions were successfully fabricated. The Nb/Al-AlOx /Nb trilayer and inpu...

Two-dimensional (2D) semiconductors offer a convenient platform to study 2D physics, for example, to understand doping in an atomically thin semiconductor. Here, we demonstrate the fabrication and unravel the electronic properties of a lateral doped/intrinsic heterojunction in a single-layer (SL) tungsten diselenide (WSe2), a prototype semiconducti...

An intermediate shuttling structure of the chloroaluminum phthalocyanine(ClAlPc)-based molecular switch is transiently created and analyzed by combined scanning tunneling microcopy/spectroscopy and density-functional theory calculations, which suggests that the Cl atom...

Probiotics, as dietary supplements, transmit their major effects through the regulation of the gut microbiota. According to the previous study, one possible mechanism of Lactobacillus casei CCFM419 protection against diabetes may involve the gut flora. To test this hypothesis, high fat and streptozotocin-induced C57BL/6J mice were fed L. casei CCFM...