Wanda Viegas

Wanda Viegas
University of Lisbon | UL · Centro de Botânica Aplicada à Agricultura (CBAA)

About

219
Publications
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3,059
Citations
Citations since 2016
72 Research Items
1147 Citations
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2016201720182019202020212022050100150
2016201720182019202020212022050100150

Publications

Publications (219)
Article
Full-text available
In the last 2 years, the COVID-19 pandemic has spread all over the world, forcing the closure of universities, among other unusual measures in recent history. (1) Background: This work is based on the study HOUSE-ULisbon, a survey carried out during the second confinement (March–May 2021) in Portugal with the collaboration of all the Faculties of t...
Article
Full-text available
Students with chronic conditions (CC) tend to experience several barriers in terms of their school participation and performance. Therefore, the present study aims to explore the factors related to the time of diagnosis of CC (recent/non-recent), the barriers to participation and academic success (health condition, people’s attitude towards CC and...
Article
Full-text available
High temperature (HT) events have negative impact on wheat grains yield and quality. Transcriptome profiles of wheat developing grains of commercial genotypes (Antequera and Bancal) and landraces (Ardito and Magueija) submitted to heatwave-like treatments during grain filling were evaluated. Landraces showed significantly more differentially expres...
Chapter
It is today well acknowledged that pedagogical approaches based on Inquiry methods and Research and Innovation (RRI) topic and processes can improve the interest of students in studying scientific topics. Such approaches can give students the opportunity to interact with cutting edge topics, research results and work with researchers to better alig...
Article
Continuous application of Cu-based fungicides and molluscicides may lead to Cu accumulation in vineyard soils. Grapevine (Vitis vinifera L.) inoculation with arbuscular mycorrhizal fungi (AMF) can be a suitable strategy to increase plant tolerance to the excessive levels of this element in the soil. However, the simultaneous use of EDTA-based ferti...
Article
Full-text available
Wheat is a dietary staple consumed worldwide strongly responsible for proteins and carbohydrate population intake. However, wheat production and quality will scarcely fulfill forward demands, which are compounded by high-temperature (HT) events as heatwaves, increasingly common in Portugal. Thus, landraces assume crucial importance as potential res...
Article
Full-text available
Purpose This paper aims to understand if a project work methodology proposed to students, based on an inquiry perspective and dealing with different dimensions of sustainable development, contributed to creating an interdisciplinary solution for a problem on sustainability challenged by food production and consumption, and also to understand if thi...
Article
Full-text available
Heat stress negatively affects several physiological and biochemical processes in grapevine plants. In this work, two new methods, calorespirometry, which has been used to determine temperature adaptation in plants, and near-infrared (NIR) spectroscopy, which has been used to determine several grapevine-related traits and to discriminate among vari...
Conference Paper
Full-text available
Three wild pear species (Pyrus cordata, P. pyraster and P. bourgaeana) that occur naturally in Portugal, were studied to characterize their genetic diversity and to assess the status of native populations for genetic conservation. Three different analytical systems were used: fruits and leaves morphological characteristics, genetic analysis by micr...
Article
Full-text available
Wheat is one of the most important cereals for food and feed, and it is, therefore, necessary to determine the effects of short-term high temperature events (heatwaves) during grain filling. These heatwave events are increasingly common, especially in Portugal. In this work, seven commercial varieties recommended for production in Portugal were sub...
Preprint
RNA editing challenges the central dogma of molecular biology, by modifying the genetic information at the transcription level. Recent reports, suggesting increased levels of RNA editing in plants, raised questions on the nature and dynamics of such events during development. We here report the occurrence of distinct RNA editing patterns in wild Vi...
Article
Full-text available
Wheat is undoubtedly one of the most important crops worldwide and it is essential to study how the distinct varieties answer to heat waves associated with climatic changes, in order to design adequate wheat breeding strategies. To assess high temperature (HT) impact in wheat grain characteristics, seven commercial varieties, which have been recomm...
Article
Full-text available
A rich heritage of traditional pear varieties is kept in national Portuguese collections. Out of these varieties, “Rocha” dominates national pear production. Although a noticeable phenotypic variation among clones of this variety has been reported, little is known about its genetic variability, as to date molecular studies have been performed on a...
Article
Assessing durum wheat genomic diversity is crucial in a changing environmental particularly in the Mediterranean region where it is largely used to produce pasta. Durum wheat varieties cultivated in Portugal and previously assessed regarding thermotolerance ability were screened for the variability of coding sequences associated with technological...
Article
Full-text available
Plant inoculation with arbuscular mycorrhizal fungi (AMF) is increasingly employed to enhance productivity and sustainability in agricultural ecosystems. In the present study, the potential benefits of AMF inoculation on young grapevines replanted in pots containing vineyard soil with high Cu concentration were evaluated. For this purpose, one-year...
Article
Several large-scale metabolic profiling studies have been directed to prospect crops with a major focus on yield-related traits and, ultimately, with the definition of specific markers for plant selection in breeding programs. However, some of these technologies are expensive, time-consuming and not easily feasible for a quick approach. Fatty acid...
Presentation
Tomato cultivation worldwide runs on excessive inorganic nitrogen and phosphate drip-fertilization ultimately leading to soil erosion and degradation, decrease of water quality and organic matter mineralization as well as an accumulation of nitrates in the surface and groundwater. Therefore, sustainable practices are needed to maintain tomato yield...
Presentation
Full-text available
During the last decades, agriculture in Sub-Saharan Africa (SSA) has been marked by dramatic shifts with the coverage of single crops, increasing pressure over the available arable land, at the expense of deforestation and soil degradation. This agricultural regime although productive in a short run, is a liability in a long run, due to the decreas...
Presentation
Mediterranean economic and socioeconomic events have contributed to the process of land marginalization. Decreasing interest in agriculture by the younger generation associated with decreasing and uncertainty income from the sector has increased marginal areas. These lands are usually filled with dead leaves and branches, dry bushes and shrubs. Cli...
Article
Full-text available
The predicted world population increase along with climate changes threatens sustainable agricultural supply in the coming decades. It is therefore vital to understand crops diversity associated to abiotic stress response. Heat stress is considered one of the major constrains on crops productivity thus it is essential to develop new approaches for...
Data
Accession numbers. Accession numbers of TdHSP101C coding sequence for the C-terminal region of the corresponding protein of distinct Portuguese durum wheat varieties. (PDF)
Data
TdHSP101C inter-varietal comparison. Inter-varietal comparison of TdHSP101C partial sequences and comparison between the two forms identified in Portuguese durum wheat varieties. (PDF)
Data
TdHSP101C intra-varietal comparison. Intra-varietal comparison of TdHSP101C partial sequences in Portuguese durum wheat varieties. (PDF)
Chapter
Arbuscular mycorrhizal (AM) symbiosis is one of the most widespread and ancient symbiosis between plants and microorganisms, conferring multiple benefits to the plant such as better nutrient and water uptake and increased biotic and abiotic stress tolerance. The establishment of a successful symbiosis involves a complex and highly regulated cellula...
Data
Accession numbers. Accession numbers of Avena sp. 45S rDNA intergenic sequences. (PDF)
Data
Avena sp. 5’ ETS sequences. Alignment of complete and partial Avena sp. 5’ ETS sequences with the published sequence of A. sativa between 2320 and 4098 bp (Accession Number: X74820.1). Complete sequences obtained from Avena strigosa, A. barbata, A. murphyi, A. sativa and A. stetilis (strI to steII), partial sequences obtained with primer ETS2_for f...
Data
A. sterilis shorter IGS. Consensus sequence of A. sterilis shorter intergenic spacer sequence. (PDF)
Presentation
Full-text available
During the last decades, agriculture in West Africa has been marked by dramatic shifts with the coverage of single crops, increasing pressure over the available arable land. Yet, West African countries are still striving to achieve sustainable production at an increased scale for global market needs. Market-driven rapid intensification is often a m...
Article
Full-text available
The genus Avena comprises four distinct genomes organized in diploid (AA or CC), tetraploid (AABB or AACC) and hexaploid species (AACCDD), constituting an interesting model for phylogenetic analysis. The aim of this work was to characterize 45S rDNA intergenic spacer (IGS) variability in distinct species representative of Avena genome diversity-A....
Article
Background and aims Rye supernumerary (B) chromosomes have an accumulation mechanism involving the B subtelomeric domain highly enriched in D1100- and E3900-related sequences. In this work, the effects of heat stress during the early stages of male meiosis in 0B and +B plants were studied. Methods In-depth cytological analyses of chromatin structu...
Article
Full-text available
Rye (Secale cereale L.) is a cereal crop of major importance in many parts of Europe and rye breeders are presently very concerned with the restrict pool of rye genetic resources available. Such narrowing of rye genetic diversity results from the presence of “Petkus” pool in most modern rye varieties as well as “Petkus” × “Carsten” heterotic pool i...
Article
Bisphenol A (BPA) is capable of mimicking endogenous hormones with potential consequences for human health and BPA exposure has been associated with several human diseases including neuropsychiatric disorders. Here, quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) results show that BPA at low concentrations (10 ng/mL and 1 μg/mL) induces...
Article
Full-text available
Faba bean (Vicia faba L.) is a facultative cross-pollinating legume crop with a great importance for food and feed due to its high protein content as well as the important role in soil fertility and nitrogen fixation. In this work we evaluated genetic diversity and population structure of faba bean accessions from the Western Mediterranean basin an...
Data
Isolation by distance test for all cultivated, and only Portuguese accessions. Testing Isolation-By-Distance (IBD) by plotting pairwise geographic distances against pairwise FST (left) and pairwise genetic distances (D) (right) for each pair of accessions genotyped in the cultivated panel only (A-B) and the Portugal only accessions (C-D). Regressio...
Data
PCA analysis of allele frequencies for the Portuguese faba bean accessions. Lettuce Plot of the 1st to 5th components of a PCA analysis based on the accession allele frequencies of polymorphic SSR markers for the Portuguese faba bean accessions. See Fig 2 for legend. (TIF)
Data
Consensus Neighbour-Joining tree for cultivated accessions. Consensus boot-strapped Neighbour-Joining tree between faba bean accessions based on the allele frequencies of polymorphic SSRs for cultivated accessions. The tree was constructed from Nei’s (D) genetic distances with 100 bootstrap replicates. The number of times the same node is retrieved...
Data
SSR markers used in this study, including primer sequences and expected sizes. (XLSX)
Data
Pairwise FST and pairwise genetic distance matrices for each pair of accessions. Each spreadsheet represents one of the three different levels of analysis: all cultivated and wild accessions; cultivated accessions; Portuguese accessions (XLSX)
Data
Evanno’s ΔK calculations for the STRUCTURE runs with different accession panels. (XLSX)
Data
Geographical distribution of population structure in Portuguese individual. Geographical distribution of population structure in Portuguese individual faba plants according to the models K = 2 (upper map) and K = 7 (lower map) produced by STRUCTURE. Each individual is depicted as a pie chart with the proportional membership of its alleles to each o...
Data
Correlations between inbreeding coefficient (F) for the cultivated accessions and four climatic variables. Clockwise from upper left: average daily temperature (°C), average yearly rainfall (mm), average highest temperature (°C) and average lowest temperature (°C). Regression line and r2 values are shown in blue. (TIF)
Data
PCA analysis of allele frequencies for the all wild and cultivated faba bean accessions. Lettuce Plot of the 1st to 4th components of a PCA analysis based on the accession allele frequencies of polymorphic SSR markers for the all wild and cultivated faba beanaccessions. See Fig 2 for legend. (TIF)
Data
PCA analysis of allele frequencies for the all cultivated faba bean accessions. Lettuce Plot of the 1st to 5th components of a PCA analysis based on the accession allele frequencies of polymorphic SSR markers for the cultivated faba bean accessions. See Fig 2 for legend. (TIF)
Data
Consensus Neighbour-Joining tree for Portuguese accessions. Consensus boot-strapped Neighbour-Joining tree between faba bean accessions based on the allele frequencies of polymorphic SSRs for Portuguese accessions. The tree was constructed from Nei’s (D) genetic distances with 100 bootstrap replicates. The number of times the same node is retrieved...
Data
List of individuals analysed and their alleles for each SSR. (XLSX)
Data
Genetic diversity measures for the SSR markers screened in the three different levels of analysis. (XLSX)
Data
Genetic diversity measures for the accessions genotyped in the three different levels of analysis. Included are information about accessions (species, geographic coordinates, country provenance, type, region of origin). (XLSX)
Article
Full-text available
Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecula...