Wahyu Widoretno

Wahyu Widoretno
Brawijaya University · Department of Biology

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18
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Publications

Publications (18)
Conference Paper
Porang is a member of the genus Amorphophallus that has high economic value because of its glucomannan content. Glucomannan is a fiber carbohydrate that is beneficial for health, for example, it can be used to control obesity. Currently, the cultivation area of porang is increasing. However, the availability of seeds is not sufficient for land expa...
Article
Full-text available
The aim of the research was to obtain the method of porang’s tuber dormancy breaking. The source of porang’s tuber were obtained from Rejosari Village, Bantur Subdistrict, Malang Regency. The diameter and weight of porang’s tuber were 5-7 cm and 100-150 g respectively. The research design was Completely Randomized Design. Porang’s tubers were stora...
Article
Hyperhydricity is a symptom of abnormal morphological and physiological function which inhibits the regeneration of plantlets. In general, the main symptom of hyperhydricity is a change in the condition of the plantlets which looks clear (Glassy) as a result of low levels of chlorophyll, the high water content in the plantlets, and the abnormal ana...
Article
Full-text available
Sunaryo W, Widoretno W, Nurhasanah, Sudarsono. 2016. Drought tolerance selection of soybean lines generated from somatic embryogenesis using osmotic stress simulation of poly-ethylene glycol (PEG). Nusantara Bioscience 8: 45-54. Somaclonal variation is an alternative source to create genetic variability including generating a novel character like d...
Article
Citrus mandarin Batu 55 (Citrus reticulata Blanco.) is one of Indonesian fruits commodities that have high economic value and consumers demand. The propagation of citrus mandarin by plant tissue culture generally was carried out on solid medium. The liquid culture system could increase cell multiplication therefore it became alternative method of p...
Article
The present study was conducted to investigate the genetic kinship of tongkat langit banana population from Moluccas which was out-grouped into Musa fehi, Ensete glaucum, Ensete superbum dan Ensete ventricosum (NCBI). The results of phylogenetic analysis showed that tongkat langit banana and Musa fehi formed sub-clade I with similarity value of 99-...
Article
The effect of gibberellin at multiplication stage on somatic embryo growth and maturation and plantlet regeneration of tangerine (Citrus reticulata Blanco.) var. Batu 55 was assessed. Somatic embryo at globular phase was cultured on MT media + 30 gL⁻¹ sucrose and various concentrations of gibberellin (0, 2, 4, 6, and 8 ppm). The somatic embryo was...
Article
This study was aimed to determine the effect of incubation at 4°C temperature during multiplication stage of somatic embryos on growth, maturation and plantlets regeneration of citrus Mandarin var Batu 55. Globular somatic embryos were cultured on MT (Murashige & Tucker) medium and incubated at 4°C temperature for 0, 2, 4, 6, 8 weeks. Maturation of...
Article
The study aims to determine the phenotypic variation of Fei banana from the Maluku islands. Exploration was conducted at the island of Ambon, Saparua, Nusalaut, Haruku, Seram, Dobo, Wokam, Tual, Elat, Tobelo, Jailolo. Phenotypic characterization of vegetative and generative organs of Fei banana (Musa troglodytarum L.) was done using the reference o...
Article
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Results of in vitro selection for tolerant to Poly Ethylene Glycol (PEG) on soybean do not always give the appearance of dry resistant when tested in the field. This research was conducted to evaluate the yield potential of 20 soma clone variants resulted from in vitro selection when planted under drought stress condition in the field. Field test w...
Article
Full-text available
In soybeans, drought stress causes 50% yield losses. Breeding for drought tolerance in soybeans has been widely developed using various methods, among which is polyethylene glycol (PEG-6000) induction to simu-late drought in vitro. In a previous experiment, three somaclones with different levels of tolerance were ge-nerated. The objectives of this...
Article
Full-text available
Water stress due to drought is the most signifi cant abiotic factor limiting crop growth and development. Drought stress inhibit germination and seedling growth, and extention of inhibition in germination under drought stress condition is varying in varieties. The previous research result showed that the differences in germination response under dr...

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