Wael A. Khalil

Wael A. Khalil
Faculty of Agricultre, Mansoura University · Animal Production Department

PhD

About

86
Publications
20,735
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1,000
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Additional affiliations
February 2010 - November 2010
Royal Veterinary College
Position
  • Professor (Assistant)

Publications

Publications (86)
Article
Full-text available
Phytochemicals have been effectively used to enhance the growth and productivity of farm animals, while the potential roles of essential oils and their nano-emulsions are limited. This plan was proposed to investigate the impacts of orally administered moringa oil (MO) or its nano-emulsion (NMO) on the growth, physiological response, blood health,...
Article
Epidermal growth factor (EGF) plays a crucial role in maintaining male reproductive capacity in mammals, however, its protective effects on cryopreserved dromedary camel epididymal spermatozoa have not been thoroughly investigated. This study aims to investigate the potential protective role of EGF on cryopreserved camel epididymal spermatozoa, sup...
Article
Full-text available
This experiment was conducted to determine the most suitable glycerol concentration (3 or 6%) and/or non-penetrating cryoprotectants (trehalose and sucrose) for the cryopreservation of buffalo semen, with the aim of enhancing the cryopreservation protocol. Semen of Egyptian buffalo were pooled and diluted with eight Tris extenders supplemented with...
Article
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This study was conducted to investigate the effects of Sodium selenite (Se) and zinc sulfate (Zn) supplementation individually and blended between them on semen quality, testicle size, weight, and Histological changes of the testis and epididymis, in 12 Kurdi rams. The twelve rams were divided randomly into 4 equal groups. The animals were divided...
Article
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Nanomaterials offer several promising prospects in the field of farm animal reproduction, encompassing a broad range of applications such as transgenesis and the precise delivery of substances to sperm cells, antimicrobial, antioxidants properties as well as their potent role in improving cryopreservation methods. The aim of the present study is to...
Article
Sperm cryopreservation can lead to subfertility due to potential damage to sperm DNA, membranes, and overall motility caused by the freeze-thaw process. Interleukin-6 (IL-6) is a versatile cytokine with various roles in reproductive processes. However, the impacts of IL-6 supplementation on cryopreserved ram sperm have not been thoroughly investiga...
Article
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Cryopreservation has adverse effects on sperm function and structure, while nanotechnology can be used to optimize this process. Thereafter, this research aimed to investigate the effects of adding spirulina platensis nanoparticles (SPNP) or selenium-coated spirulina nanoparticles (SPSE) to a freezing extender on the quality of post-thawed bull spe...
Article
The current study aimed to investigate the impact of nano‐formulations of clove bud ethanolic extract (CBENF) in the extender on sperm characteristics, antioxidant capacity, oxidative biomarkers, enzymatic activity, apoptosis and fertility of post‐thawed rabbit semen. Twelve mature male rabbits semen samples were pooled and cryopreserved in a Tris‐...
Article
Sperm preservation is a well-established technique in reproductive biotechnology that is widely used to maintain the genetic quality of male individuals. However, there are several factors during the preservation process that can affect the vitality, functionality, and quality of sperm, thereby reducing their fertility potential after thawing. One...
Article
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Thymoquinone nanoparticles (TQNPs) are broadly utilized in numerous pharmaceutical applications. In the present study, we tested the effects of TQNP supplementation on sperm quality and kinematics, acrosome exocytosis, oxidative biomarkers, apoptosis-like and morphological changes of frozen–thawed buffalo sperm, as well as the fertilizing capacity....
Article
The existing treatise targeted to compare the effects of adding different nano-emulsions essential oils (olive, flaxseed, and grapeseed oils) in freezing extender on semen quality and freezability in buffalo. Nano-emulsions were prepared from olive, flaxseed, and grapeseed oils and characterized for their sizes and shapes. Semen extended in four tu...
Article
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Abstract Buffalo sperm is sensitive to cryoinjuries, thus improving sperm cryoresistance is a critical approach for wide spreading the assisted reproductive technologies in buffalo. The intention of this work was to assess the effect of propolis-loaded in nanoliposomes (PRNL) supplementation of semen extender on semen quality, antioxidant status an...
Article
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This study was conducted to study the effect of adding selenium and zinc individually and their mixture on the carcass characteristics of male Kurdi lambs. The study included 16 male Kurdi lambs aged between 4-5 months and weights between 20-23 kg, the animals were divided into four groups, 4 lambs per group. The first group (the control) was fed a...
Article
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Portulaca oleracea contains a wide range of chemical constituents, including flavonoids, alkaloids, polysaccharides, fatty acids, terpenoids, sterols, proteins, vitamins, and minerals. These chemical constituents are linked to the plant's diverse pharmacological properties, such as antimicrobial and antioxidant activities. Therefore, this study is...
Article
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Estrous synchronization is a promising management device to get better reproductive proficiency, particularly in ruminants. However, limited information is available about the influence of long and short-term proges-terone administration on estrous synchronization and reproductive performance in ewes during May season, particularly in Egypt. In thi...
Article
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This research was designed to explore the protective effect of alpha-lipoic acid–loaded nanoliposomes (ALAN) during cryopreservation of buffalo sperm. Buffalo semen was cryopreserved in a tris-citrate egg yolk extender without any supplement (ALAN0, control group) or with ALAN at levels of 25, 50, 75 or 150 µg (ALAN25, ALAN50, ALAN75 and ALAN150, r...
Article
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Rabbits are sensitive to heat stress (HS) in hot regions due to difficulty in eliminating excess body heat. We evaluated the ameliorating role of black maca hydroalcoholic extract (BMHE) against HS conditions on the productive and reproductive performance of V-line rabbit does. Rabbits were divided into four equal groups (T1–T4), each containing th...
Article
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Bee bread has numerous nutritional benefits and bioactive compounds. Other bee by-products have been used as extender additives to improve semen cryopreservation. Here, we examined the effects of supplementing egg yolk extender (EYE) or soybean lecithin extender (SBLE) with bee bread extract (BBE) on the quality of cryopreserved ram semen. Semen wa...
Article
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This study evaluated the efficacy of Turraea fischeri leaf extract for maintaining the viability of cryopreserved goat sperm. Ejaculated semen was collected from 5 mature Baladi bucks (50-60 kg, 2-4 years of age) and those samples with mass motility ≥ 70% and sperm concentration ≥ 2.5 × 10 9 /mL were selected, pooled, and divided into 4 aliquots. E...
Article
The aim of the present study was to investigate the effect of supplementing rabbit semen extender with zeolite loaded with different charges (Z⁺ or Z⁻, Z±) on sperm cryopreservation. Semen was collected from six healthy, fertile New Zealand rabbit bucks using an artificial vagina. The collected ejaculates were pooled and diluted with a tris-yolk fr...
Article
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Twenty five non seasonality (non pregnant) adult Barki ewes were used to investigate the effect of addition of various levels of rumen protected methionineon on some wool characteristics. This study was conducted from September 2019 to March 2020. Animals were divided according to live body weight and the similarity of wool measurements. The contro...
Article
This study examined the effects of zinc chloride (ZnCl2) and sodium selenite (Na2SeO3) supplementation in maturation media on in vitro maturation (IVM) rate, oxidative biomarkers and gene expression in buffalo oocytes. The work focused on antioxidant, antiapoptotic and proapoptotic genes. Ovaries from a slaughterhouse were aspirated and good qualit...
Article
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This study determined the effects of antioxidant supplementation and storage time at cool temperatures on the characteristics of epididymal camel spermatozoa. Camel testes were collected at the abattoir after animal slaughtering and kept at 4 °C during transportation and until processing (max 6 h). Spermatozoa were retrieved and diluted with SHOTOR...
Article
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Simple Summary: Long-term preservation of semen is a pivotal step for artificial insemination in most farm animal species, but it is associated with cellular insults at the cell membrane and cytoskeleton level as well as the generation of reactive oxygen species (ROS). We highlight the recent strategies to combat these negative effects through defe...
Article
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The Splinter bean, Entada abyssinica, is widely used in folk medicine. In the current work, we profiled the secondary metabolites from E. abyssinica bark extract using LC-MS and investigated its effect on cryopreserved ram semen. Twenty-eight compounds, including tannins and gallic acid derivatives that prevailed in the extract, were tentatively id...
Article
Goat semen cryopreservation is a challenging process as it results in reduced motility, vitality, and fertility of spermatozoa after freezing. In this study, we evaluated the effects of different herbal extract nanoformulations (NFs) [mint (MENFs), thyme (TENFs), and curcumin (CENFs)], supplemented at either 50 or 100 μg into Tris-extender on the c...
Data
Graphical Abstract: Mechanisms involved the pathogenesis of renal I/R injury and possible renoprotective mechanisms of palm date extracts. Renal ischemia for 45-min caused loss of renal tubular epithelial cells by necrosis and apoptosis via upregulation of the free oxygen radicals, inflammatory cytokine TNF-α. Also, it enhanced the expression of nr...
Article
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Increased reactive oxygen species (ROS) generation may disrupt the oocytes function and their competence. In this study, we introduced BTZO‐1, a new supplement that can regulates the oxidative stress. Addition of BTZO‐1 during IVM of bovine oocytes improved their developmental competence in the term of improvement of blastocyst rates. In addition,...
Article
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There are various challenges during semen cryopreservation used for artificial insemination, oxidative stress is considered as the major factor. Usage of nano-particle materials is an efficient tool to overcome these challenges. In the present article, effects of adding green synthesized gold nanoparticles (GSGNPs, 5; 10ppm/mL) to Tris-extender on...
Article
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Objective: To test the effects of extenders and packaging methods on morphological and functional characteristics of frozen thawed Ossimi ram semen. Methods: Ram semen was pooled, diluted in 3 different extenders: Tris-egg yolk (TEY), Tris-soybean lecithin (TSBL), and Tris- butylatedhydroxytoluene (TBHT), equilibrated at 5 °C for 4 h, and packaged...
Article
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This study was conducted to evaluate whether adding Moringa oleifera dry leaves powder (MODLP) as a natural anti-oxidants in the diet, has the potential to attenuate the negative effects of heat stress on growth performance, healthy status, anti-oxidant defense system and immunity of growing rabbits. Weaned NZW rabbits (n=120) were divided into fou...
Article
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There are several obstacles in camel semen cryopreservation; such as increasing semen viscosity and the reduction in motile spermatozoa after ejaculation. Epididymal spermatozoa offer an efficient alternative to overcome these problems and are well-suited for artificial insemination in camels. In the current study, we compared the effects of supple...
Article
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The current study aimed to evaluate the effect of dietary supplementation with sunflower oil (SFO), flaxseed oil (FSO) or 1% SFO+1% FSO on growth performance, and lipid, testosterone and fatty acid profiles of California rabbit bucks. Number of 24 California bucks having 5.3-5.5 months of age and weighing 2316-2429 g were divided into four groups (...
Article
Full-text available
This study examined the effects of zinc chloride (ZnCl 2 ) and sodium selenite (Na 2 SeO 3 ) supplementation in maturation medium on in vitro maturation (IVM) rate, oxidative biomarkers and gene expression in buffalo oocytes. Ovaries from a slaughterhouse were aspirated and good quality cumulus–oocyte complexes (COCs) with at least four layers of c...
Article
The aim of this study was to evaluate the effect of adding different concentrations of selenium nano-particles (Se-NPs) in semen extender on bull sperm cryopreservation. Five healthy, fertile Friesian bulls were used, and the ejaculates were obtained using an artificial vagina. Semen of all bulls were pooled and diluted in a tris-yolk fructose (TYF...
Article
Full-text available
Recent advances in nanotechnology have tremendously expanded its possible applications in biomedicine. Although, the effects of nanoparticles (NPs) at cellular and tissue levels have not been fully understood, some of these biological effects might be employed in assisted reproduction to improve male fertility particularly by enhancing sperm cell q...
Article
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Effect of Some Alternative Components of Egg Yolk in Tris-Extender on Sperm Characteristics of Ram Semen Frozen with Two Methods of Packaging Semen Abdel-Khalek, A. E.1; W. A. Khalil 1; B. E. El-Saidy2and A. I. A. Yousif 2 1Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt 2Animal Production Researc...
Article
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This study designed to examine effects of adding different concentrations of propolis ethanolic extract (PEE) as antioxidant to ram semen extenders; tris egg yolk (TEY) or tris-soybean lecithin (TSBL) on sperm characteristics, lipid peroxidation and enzymatic activities in seminal plasma of ram semen preserved at 5°C for 48 hours. Semen was collect...
Article
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Dietary polyunsaturated fatty acids (PUFAs) can influence fertility in farm animals. Some evidence in mice and sheep have suggested that PUFAs may influence offspring sex ratio, which may have significant value for cattle production. To test this hypothesis, three groups of Holstein cows were supplemented with either 0%, 3% or 5% protected fat (PF)...
Article
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Aim of this study was to investigate performance and puberty of ram lambs produced from pregnant ewes treated with different levels of arginine. This study was carried out at Sakha Animal Production Research Station, belonging to the Animal Production Research Institute in a cooperation with Animal Production Department, Faculty of Agriculture, Man...
Article
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Albizia harveyi is a tropical deciduous tree, found across South and Eastern Africa and widely used in traditional medicine. The leaf extract ameliorated the damaging effects of the frozen-thawing process in cryopreserved bull semen. In a dose-dependent pattern, sperm motility, viability, and membrane integrity were improved compared to the untreat...
Article
Full-text available
Semen cryopreservation is a well-established procedure used in veterinary assisted reproduction technology applications. We investigated damaging effects of cryopreservation on the structural and ultrastructural characteristics of bull sperm induced at different temperatures and steps during standard cryopreservation procedure using transmission (T...
Article
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Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to p...
Article
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This study aimed to investigate effect of addition of different levels (0, 100, 500 and 1000 µg/ml) from Moringa oleifera leaves (MOL) or Arctium lappa L. roots (ALR) extracts, as antioxidants to ram semen extender on sperm characteristics, lipid peroxidation and enzyme activity in seminal plasma of ram semen preserved at cool temperature (5°C)for...
Article
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The aim of the present study was to define the effect of different extender components (as conventional and non-conventional extenders) on rams' semen quality during storage at 5ºC for up to 6 days. Fertility rates of the extended ram spermatozoa stored at 5ºC for up one day were also studied. Four Rahmani rams were used to collect pooled ejaculate...
Article
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A r t i c l e I n f o A feeding trial for 60 days was carried out on rabbits to study the effects of 1,4-dioxane (3.2mg/Kg) including diets with or without vitamin C (1g/Kg diet, as antioxidant) on their blood profile and some internal organs' histology (kidney, liver and testis). From the obtained blood results and histological examination, it cou...
Article
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Abstract This study was conducted to determine the effect of different type and levels of antioxidant supplementation (0.4 and 0.8 mM from glutathione, GSH or 0.5 and 1.0 g LG1 extender from ascorbic acid, AA) as compared to control, on characteristics of camel epididymal spermatozoa stored at 25EC (room temperature) for 0, 2, 4 and 12 h or at 5EC...
Article
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The current study was carried out at Animal Experimental Station and the Laboratory of Physiology and Biotechnology, belonging to the Animal Production Department, Faculty of Agriculture, Mansoura University, during the period from September to December 2014. The aim of this experiment is to study the effect of daily oral administration of free L-c...
Article
The current study was carried out at Animal Experimental Station and the Laboratory of Physiology and Biotechnology, belonging to the Animal Production Department, Faculty of Agriculture, Mansoura University, during the period from September to December 2014. The aim of this experiment is to study the effect of daily oral administration of free L-c...
Article
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This study aimed to compare the efficiency of three cryodevices (Conventional Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They were vitrified and thawed according to each cryodevice used. Then they were in vitro matured, fertilized and cultured to...
Article
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Total of 40 crossbred ewes were divided into five treatment groups (8 ewes each). Ewes in the first group (G1) represented the control ewes which were allowed for natural mating. The control ewes were exposed to fertile ram from the contemporary to that of treatment groups start time up to the end of the breeding season (end of September) and natur...
Article
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Optimization of in vitro oocyte maturation conditions is crucial to maximize the number and quality of the transferable embryos. A demand for optimised chemically defined, serum-free medium for this purpose is increasing. Polyunsaturated fatty acids (PUFAs) have been shown to influence oocyte competence. Many ruminant diets are rich in the omega 6...
Article
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ABSTRACT The main objective of this study was to evaluate the effect of supplementing maturation media with, retinol, ascorbic acid and α-tocopherol at a level of 100 µmol/ml for each, on in vitro cytoplasmic and nuclear maturation of buffalo oocytes. Oocytes were collected from ovaries taken from slaughterhouses. Only compact oocytes were used for...