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Volodymyr A Vinnikov

Volodymyr A Vinnikov
Grigoriev Institute for Medical Radiology and Oncology of the National Academy of Medical Sciences of Ukraine

PhD

About

42
Publications
5,392
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369
Citations
Citations since 2016
18 Research Items
251 Citations
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201620172018201920202021202201020304050
201620172018201920202021202201020304050

Publications

Publications (42)
Article
Full-text available
External beam radiation therapy leads to cellular activation of the DNA damage response (DDR). DNA double-strand breaks (DSBs) activate the ATM/CHEK2/p53 pathway, inducing the transcription of stress genes. The dynamic nature of this transcriptional response has not been directly observed in vivo in humans. In this study we monitored the messenger...
Article
Radiation dosimetric biomarkers have found applications beyond radiation protection area and now are actively introduced into clinical practice. Cytogenetic assays appeared to be a valuable tool for individualized quantifying radiation effects in patients, with high capability for assessing genotoxicity of various medical exposure modalities and pr...
Article
Background A detonation of nuclear weapon (NW) is considered as one of the most devastating radiological scenarios in the list of modern global threats. An essential proportion of victims in a mass casualty radiation event may require an immediate medical care due to radiation combined injuries (RCI). Surprisingly, there is a lack of clear guidance...
Article
Full-text available
Background. Among cancer patients receiving radiotherapy about 5–15 % may have adverse reactions in normal tissues and organs that limit their treatment in a full, originally scheduled regimen. The development of biomarkers and assays for radiation oncology allowing the prediction of patients’ normal tissue toxicity requires a lot of resourses, thr...
Article
Full-text available
A search for effective methods for the assessment of patients' individual response to radiation is one of the important tasks of clinical radiobiology. This review summarizes available data on the use of ex vivo cytogenetic markers, typically used for biodosimetry, for the prediction of individual clinical radiosensitivity (normal tissue toxicity,...
Article
The strategy toward personalized medicine in radiation oncology, nuclear medicine, and diagnostic and interventional radiology demands a specific set of assays for individualized estimation of radiation load for safety concerns and prognosis of normal tissue reactions caused by ionizing radiation. Apparently, it seems reasonable to use validated ra...
Article
Full-text available
The strategy toward personalized medicine in radiation oncology, nuclear medicine, and diagnostic and interventional radiology demands a specific set of assays for individualized estimation of radiation load for safety concerns and prognosis of normal tissue reactions caused by ionizing radiation. Apparently, it seems reasonable to use validated ra...
Article
This study provides an improved background for the operational assessment of the radiation hazardous areas with induced radioactivity, based on the analysis of spectral characteristics of the neutron component of the penetrating radiation. The interrelationships between the nuclear munitions explosion parameters, environmental conditions and dosime...
Article
Dosimetric biomarkers have been effectively and intensively used for a long time in the area of radiation protection. In contrast to that, no robust standards or widely accepted protocols for application of these end-points in radiotherapy, diagnostic and interventional radiology and nuclear medicine exist to date. The International Atomic Energy A...
Article
Full-text available
Dosimetric biomarkers have been effectively and intensively used for a long time in the area of radiation protection. In contrast to that, no robust standards or widely accepted protocols for application of these end-points in radiotherapy, diagnostic and interventional radiology and nuclear medicine exist to date. The International Atomic Energy A...
Article
Full-text available
Previous investigations in gene expression changes in blood after radiation exposure have highlighted its potential to provide biomarkers of exposure. Here, FDXR transcriptional changes in blood were investigated in humans undergoing a range of external radiation exposure procedures covering several orders of magnitude (cardiac fluoroscopy, diagnos...
Article
Full-text available
Previous investigations in gene expression changes in blood after radiation exposure have highlighted its potential to provide biomarkers of exposure. Here, FDXR transcriptional changes in blood were investigated in humans undergoing a range of external radiation exposure procedures covering several orders of magnitude (cardiac fluoroscopy, diagnos...
Article
Full-text available
The methodology of cytogenetic triage can be improved by optimizing a schedule of microscopy for different exposure scenarios. Chromosome aberrations were quantified by microscopy in human blood lymphocytes irradiated in vitro to ~2, 4, and 12 Gy acute ⁶⁰Co γ-rays mixed with the unirradiated blood simulating 10%, 50%, 90%, and 100% exposure and in...
Article
Full-text available
A new zero-inflated Poisson model is introduced for the estimation of partial body irradiation dose and fraction of body irradiated. The Bayes factors are introduced as tools to help determine whether a data set of chromosomal aberrations obtained from a blood sample reflects partial or whole body irradiation. Two examples of simulated cytogenetic...
Article
Full-text available
Classical methods of assessing the uncertainty associated with radiation doses estimated using cytogenetic techniques are now extremely well defined. However, several authors have suggested that a Bayesian approach to uncertainty estimation may be more suitable for cytogenetic data, which are inherently stochastic in nature. The Bayesian analysis f...
Article
The Poisson distribution is the most widely recognised and commonly used distribution for cytogenetic radiation biodosimetry. However, it is recognised that, due to the complexity of radiation exposure cases, other distributions may be more properly applied. Here, the Poisson, gamma, negative binomial, beta, Neyman type-A and Hermite distributions...
Article
Full-text available
The Effect of Radiotherapy Patients Blood Plasma on the Apoptosis Rate in Unirradiated Human Leukocytes Objectives: There are rather contradictory data concerning radiation-induced, toxic bystander effects mediated by human irradiated blood plasma. Therefore, the aim of the present study was to assess whether cancer patients blood plasma after rad...
Article
Full-text available
The dose response for dicentrics plus centric rings and total unstable chromosome-type aberrations was studied in the first mitoses of cultured human peripheral blood lymphocytes irradiated in vitro to doses of ∼2, 4, 6, 8, 10, 16 and 20 Gy of acute 60Со gamma-rays. A dose-dependent increase of aberration yield was accompanied by a tendency to the...
Article
Following exposure to high doses of ionizing radiation, due to an accident or during radiotherapy, bystander signalling poses a potential hazard to unirradiated cells and tissues. This process can be mediated by factors circulating in blood plasma. Thus, we assessed the ability of plasma taken from in vitro irradiated human blood to produce a direc...
Article
Several examples are selected from the literature in order to illustrate combinations of complicating factors, which may occur in real-life radiation exposure scenarios that affect the accuracy of cytogenetic dose estimates. An analysis of limitations in the current statistical methods used in biodosimetry was carried out. Possible directions for f...
Article
The scientific literature concerning cytogenetic biodosimetry has been reviewed to identify the range of scenarios of radiation exposure where biodosimetry has been carried out. Limitations in the existing standardized statistical methodology have been identified and categorized, and the reasons for these limitations have been explored. Statistical...
Article
To assess possible delayed chromosomal instability (DCI) expressed as elevated chromatid breakage in cells containing previously formed chromosome type aberrations in cultured blood lymphocytes of cancer patients after radiotherapy (RT). Twenty patients treated for uterine cancer with external Co(60) RT, without chemotherapy, were selected. Blood w...
Article
Full-text available
The paper presents the results of the cohortal biodosimetry carried out in 435 Chernobyl clean-up workers, who were surveyed with the conventional cytogenetic technique in terms from several days to 10 years after the end of their duties in the Chernobyl accident exclusive zone. An empirical model of the aberrant cell dynamics was utilized for the...
Article
Full-text available
Cytogenetic analysis using the fluorescence in situ hybridisation (FISH) technique was performed late time after the Chernobyl accident in groups of liquidators, evacuees from 30 km exclusive zone, residents of radioactively contaminated areas and control donors age-matched to exposed persons. Stable and unstable chromosome type exchanges were reco...
Article
The post-irradiation changes of dicentric and centric ring levels were studied in Chernobyl liquidators using the data of 507 individual chromosomal surveys of persons sampled at different time after their activities at Chernobyl NPP accident zone. The time-effect relationship within 0-10.5 years after exposure was displayed as exponential decline...
Article
The purpose of this paper is to investigate how well various assays on blood can detect radiation dose to people exposed many years previously and, if possible, to estimate that dose. The assays were applied to persons resident close to Chernobyl in 1986. Blood samples were taken 13-15 years after the reactor accident. The assays used were the freq...
Article
Aim: the cytogenetic survey has been carried out in uterine cancer patients undergoing radiation therapy to evaluate whether the pre-irradiation tumor cryodestruction could modify the radiation-induced effects in normal tissues. Methods: the conventional cytogenetic analysis of peripheral blood lymphocytes was undertaken in cryosurgically treated a...
Article
The cytogenetic screening was carried out in 100 Chernobyl clean-up workers (liquidators) with registered doses during 1 year after exposure. The bayesian analysis has been applied for constructing the individual distribution of probability density for biological doses estimated from dicentric and centric ring yields in liquidators. The coincidence...
Article
Eighteen Ukrainian evacuees from the Chernobyl exclusive zone, twenty one inhabitants of radioactively contaminated areas of Belarus and twelve control donors age-matched to the exposed persons were investigated 14-15 years after the Chernobyl accident for chromosomal aberration yields detected in blood lymphocytes by fluorescence in situ hybridisa...
Article
Data are presented for a subset of lymphocytes characterized by FISH as missing painted chromosomal material. These lymphocytes occur in both control and irradiated subjects. These cells have a much greater frequency of one-way translocations than cells in which all of the painted chromosomal material is present. Their presence contributes to inter...
Article
Dynamics of cytogenetic anomalies in liquidators of the Chernobyl accident has been studied. Dicentric and ring frequencies appeared to be the most informative criteria of radio-induced chromosome aberrations. The rate of cell elimination with dicentrics and rings was higher in liquidators exposed to more than 25 cGy of radiation that in those expo...
Article
Full-text available
Lymphocytes from some evacuees from Prypiat and clean-up workers (liquidators) have been studied for chromosomal aberrations. When dicentric yields from subjects are pooled in various ways, by date of evacuation, date of working at Chernobyl or delay in blood sampling, the resultant mean dose estimates are higher than have been generally assumed fr...

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Projects

Projects (5)
Archived project
to increase the preparedness of biological dosimetry laboratories in IAEA Member States to react on radiation and nuclear accidents nationally and regionally.
Project
To sustain and enhance the scope of biodosimetry services to radiation medicine as well as assist with the transition to personalised medicine. https://www.iaea.org/newscenter/news/new-crp-applications-of-biological-dosimetry-methods-in-radiation-oncology-nuclear-medicine-diagnostic-and-interventional-radiology-e35010
Project
Cancers (impact factor 6.639) will have the special issue “Cancer and non-cancer effects following ionizing irradiation”. This special issue aims to serve as a forum to gather the latest developments and discuss future developments in the field of normal tissue responses to ionizing radiation exposure. “Cancer” here means cancer following occupational or environmental radiation exposure, or secondary cancer following radiotherapy. “Non-cancer effects” here means tissue reactions (deterministic effects) and other non-cancer diseases (e.g. ophthalmological, circulatory or neurological) following occupational or environmental radiation exposure, or normal tissue complications following radiotherapy. Studies on mitigation strategies, bioindicators, biomarkers, mechanistic modeling, adverse outcome pathway (AOP) approach are also relevant. We would welcome submission of any type of quality manuscripts, e.g., original manuscripts reporting biological findings (at cellular, tissue or organismal levels) or epidemiological findings (in patients, medical or other occupational workers and the public), and review manuscripts on current knowledge and future perspectives, but with explicit relevance to cancer (e.g., from the aspects of carcinogenesis, cancer radiotherapy and diagnosis). The submission portal opened on 1 October 2021 and will be closed on 31 October 2022.