Volodymyr Strashnyuk

Volodymyr Strashnyuk
V. N. Karazin Kharkiv National University · Institute of Biology; Department of Genetics & Cytology

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24
Publications
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56
Citations
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April 1988 - present
V. N. Karazin Kharkiv National University
Position
  • Professor

Publications

Publications (24)
Article
Full-text available
Abstract. In temperate regions, the fruit fl y Drosophila melanogaster (Meigen, 1830) is subject to seasonal changes in natural conditions. Insects exhibit a wide range of adaptive responses to changes in the seasons. In this study, we focused on polyteny, which plays an important role in fruit fl y development. Polytene chromosomes are the result...
Article
Full-text available
Man-made disasters, such as the accidents at the Chornobyl NPP and the Fukushima NPP-1, have raised questions about the radiation risks associated with the use of atomic energy in a new and acute way. The purpose of the study was to investigate the features of the action of natural selection in the lines of Drosophila melanogaster Meig., obtained f...
Article
Full-text available
Polyteny is an effective mechanism for accelerating growth and enhancing gene expression in eukaryotes. The purpose of investigation was to study the genetic variability of polyteny degree of giant chromosomes in the salivary glands of Drosophila melanogaster Meig. in relation to the differential fitness of different genotypes. 16 strains, lines an...
Preprint
Full-text available
Polyteny is an effective mechanism for accelerating growth and enhancing gene expression in eukaryotes. The purpose of investigation was to study the genetic variability of polyteny degree of giant chromosomes in the salivary glands of Drosophila melanogaster Meig. in relation to the differential fitness of different genotypes. 16 strains, lines an...
Article
Full-text available
Aim. The purpose of investigation was to study the seasonal selection indices and its components in the natural population of Drosophila melanogaster Meig. Methods. The material for the study was the Gaidary-spring and Gaidary-autumn lines, obtained from the natural population of Gaidary in different seasons of the year. Fecundity and mortality/sur...
Article
Full-text available
Aim. The purpose of investigation was to study the reproductive ability and polyteny degree of chromosomes in Drosophila melanogaster Meig. under the influence of various concentrations of lead nitrate. Methods. Canton-S wild-type strain was used as the material. Flies developed on standard sugar-yeast medium, to which in the experiment lead nitrat...
Article
Full-text available
The purpose of this investigation was to study the effect of acute γ-irradiation of parent adults on the endoreduplication of giant chromosomes in F1 generation of Drosophila melanogaster Meig. A wild-type Oregon-R strain was used as the material. Virgin females and males of Drosophila adults at the age of 3 days were irradiated with doses of 8, 16...
Article
Full-text available
The purpose of this investigation was to study the effect of microwave irradiation, static magnetic field and their combined action on the violation of homologous pairing of polytene chromosomes in Drosophila melanogaster Meig. Inbred line Canton-S was used as an experimental object. Microwaves with frequency 36.64 GHz and power density 1 W/m2 for...
Article
Full-text available
Aim. The purpose of investigation was to study the intensity of selection in two generations of Drosophila melanogaster Meig. after acute g-irradiation. Methods. Experiments were conducted on a wild-type Oregon-R strain. Adult flies in the age of 3 days were irradiated with bremsstrahlung gamma quanta at doses of 8 Gy, 16 Gy and 25 Gy on a linear a...
Article
Full-text available
Aim. The purpose of investigation was to study the effect of microwave irradiation of different intensity on the manifestation of the position effect variegation (PEV) in Drosophila melanogaster Meig. Methods. Experiments were carried out on mutant strain In(1)wm4, y. Microwave radiation with frequency 36.64 GHz and power density 0.01; 0.1 and 1 W/...
Preprint
Full-text available
The purpose of investigation was to study the effect of acute γ-irradiation of parent adults on the endoreduplication in Drosophila melanogaster progeny. As a material used Oregon-R strain. Virgin females and males of Drosophila adults at the age of 3 days were exposed at the doses of 8, 16 and 25 Gy. Giant chromosomes were studied at late 3rd inst...
Article
Full-text available
Aim. The purpose of investigation was to study the peculiarities of homologous conjugation of polytene chromosomes in the salivary glands of larvae in Drosophila melanogaster offspring after exposure to acute γ-irradiation. Methods. Experiments were carried out on wild type Oregon-R strain. Three-day-old flies were irradiated by brake gamma rays on...
Article
Full-text available
Aim. The purpose of investigation was to study the effect of microwave irradiation of varying intensity to lifespan in adults of Drosophila melanogaster Meig. Methods. Experiments were carried out on wild type Oregon-R strain. Two-day-old flies were irradiated by microwaves (frequency – 36.64 GHz, exposure time – 10 sec). The power density was 0.01...
Article
Full-text available
Lifespan (LS) of adults of Drosophila melanogaster in the F1 generation after a single acute exposure of parents to bremsstrahlung γ-rays was studied. Wild-type stock Oregon-R was used as the material. 3-day-old adults were irradiated with bremsstrahlung gamma-rays at doses of 8 Gr, 16 Gr and 25 Gr using the electron linear accelerator LUE-10. Irra...
Article
Full-text available
Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (frequency - 36.64 GHz, power density...
Article
Full-text available
The effects of γ�radiation at the doses of 8 Gy, 16 Gy and 25 Gy on the fertility, viability, the level of dominant lethal mutations (DLM) and the frequency of unequal crossing�over in an unstable Drosophila melanogaster Meig Bar line were investigated. Female adults were irradiated by braking gamma rays on the electron linear accelerator ELA�10. T...
Article
Full-text available
The review presents data on the molecular genetic mechanisms controlling endoreduplication. The issues concerning the activity of the main cycle cell regulators, such as cyclins, cyclin-dependent kinases, and their inhibitors, under conditions of a modified cell cycle of polytene cells are considered. Specific features of regulation at the replicat...
Article
Full-text available
We studied the influence of parental age on the degree of polyteny of giant chromosomes and expressivity of mutation eyeless in Drosophila melanogaster descendants. The parental age equal to six days exerted an adverse effect on the function of endoreduplication of giant chromosomes in straineyeless. The highest degree of polyteny was observed in d...
Article
Full-text available
1. A number of puffs specifically activated by metopren (43E, 58DE, 60B, 85D) have been identified. 2. A decrease has been established in the size of late ecdysone puffs 63E and 82 EF under the action of this hormone suggesting the involvement of JH in regression of late ecdysone puffs. 3. The dependence of effect on hormone concentration has been...
Article
The effects of the Bar (B) andwhite (w) mutations on the expressiveness of the character vestigial (vg) and the degree of polyteny of salivary gland giant chromosomes were studied in Drosophila melanogaster.Either mutation changed both the expressiveness of vestigial and the degree of chromosome polyteny. A negative association between the vg expre...
Article
Full-text available
Effects of culture density on expressivity of character eyeless and polyteny of giant chromosomes were studied in Drosophila melanogaster. Larval crowding was shown to significantly reduce eye facets. The expression of eyeless was sex-dependent: in females, this character was expressed more strongly than in males. Larval crowding resulted in a decr...
Article
Full-text available
The degree of polyteny of giant chromosomes and electrokinetic properties of cell nuclei salivary glands of Drosophila melanogaster were studied in relation to genotype and rearing temperature. Inbred and selected Drosophila lines and interlinear F1 hybrids were used. The experiments were conducted in three temperature regimes: 25 (control), 18, an...
Article
Full-text available
Four classes of cell nuclei containing polytene chromosomes of different diameters were detected in the salyvary glands of Drosophila at the stage of 0-haur prepupae. The diameter of chromosomes was shown to depend on the level of polyteny. In the selected highly inbred lines LA (low activity) and HA (high activity)and their hybrids LA x HA and HA...
Article
Full-text available
The regression of four inter-molt puffs and the activation of 14 early ecdysone puffs in Drosophila larva salivary glands from inbred high- and low-adaptability lines (HA and LA respectively) and from the hybrids (HA x LA)F1 were studied in cultivation medium containing ecdysterone. Differences in the rates of development and adaptability of lines...

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