Volodymyr Yurievich Prokopyuk

Volodymyr Yurievich Prokopyuk
Institute for Problems of Cryobiology and Cryomedicine · Department of Cryobiochemistry

PhD (Medicine)

About

130
Publications
9,848
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492
Citations
Additional affiliations
September 2020 - present
Kharkiv National Medical University
Position
  • Senior Researcher
Education
September 1996 - May 2002
Kharkiv National Medical University
Field of study
  • Obstetric and gynecology

Publications

Publications (130)
Article
Full-text available
Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity of newly synthesized nanostructured zinc carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Cytotoxicity of the microflakes was assessed against murine L929 cell line and rat mature erythrocytes....
Article
Full-text available
Introduction Tumor drug resistance and systemic toxicity are major challenges of modern anticancer therapy. Nanotechnology makes it possible to create new materials with the required properties for anticancer therapy. Methods In this research, Dextran-graft-Polyacrylamide/ZnO nanoparticles were used. The study was carried out using prostate (DU-14...
Article
Full-text available
Synthesis of metal oxide nanoparticles–polymer nanocomposites is an emerging strategy in nanotechnology to improve targeted delivery and reduce the toxicity of nanoparticles. In this study, we report biological effects of previously described hybrid nanocomposites containing dextran-graft-polyacrylamide/zinc oxide nanoparticles (D-PAA/ZnO NPs) prep...
Article
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The therapeutic potential of redox-active nanoscale materials as antioxidant- or reactive oxygen species (ROS)-inducing agents was intensely studied. Herein, we demonstrate that the synthesized and characterized GdVO4:Eu3+ and LaVO4:Eu3+ nanoparticles, which have been already shown to have redox-active, anti-inflammatory, antibacterial, and wound h...
Article
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Nanoparticles (NPs) with reactive oxygen species (ROS)-regulating ability have recently attracted great attention as promising agents for nanomedicine. In the present study, we have analyzed the effects of TiO2 defect structure related to the presence of stoichiometric (Ti⁴⁺) and non-stoichiometric (Ti³⁺ and Ti²⁺) titanium ions in the crystal latti...
Article
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Introduction: Human chemerin is an adipokine that regulates chemotaxis, inflammation, and glucose metabolism. In addition, accumulating evidence suggests that chemerin promotes apoptosis, autophagy, and pyroptosis. However, there are no data on its impact on eryptosis. The current study aimed to analyze the effects of human active Glu²¹-Ser¹⁵⁷ chem...
Article
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Genital tract infections of various etiologies and anemia remain common in pregnant women. At present, there is a paucity of data on the impact of reproductive tract infections on the development of anemia in pregnant women, with particular emphasis on the contribution of eryptosis. The aim of the current study was to assess the ability of bacteria...
Article
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The development of new diuretics of plant origin is an actual direction. Chimaphila umbellata (L.) is a perennial herb with diuretic, astringent, analgesic and other effects; and it can treat various conditions such as edema, dropsy, etc. Pipsissewa herb helps the removal of nitrogenous and chloride salts from the body due to the content of arbutin...
Preprint
Full-text available
Introduction Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity of newly synthesized nanostructured zinc carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods Cytotoxicity of the microflakes was assessed against murine L929 cel...
Article
The article analyzes the effect of different concentrations of GdYVO4 :Eu3+ nanoparticles solution, which were pre-activated by ultraviolet light and not activated, on metabolic activity of the MCF-7 cancer cell culture. It was found that the degree of changes in the state of breast cancer cells depended on the concentration of nanoparticles, namel...
Article
The research covers the specific effect of human umbilical cord cryoextract and the culture medium of its mesenchymal stromal cells in comparison with synthetic hyaluronic acid on the skin regeneration in female rats with simulated aging. The specified substances were administered intradermally to ovariectomized animals. The injection of human umbi...
Article
Full-text available
Unlabelled: Aim In the current study, hemocompatibility of three major commercially available types of carrageenans (ι, κ and λ) was investigated focusing on eryptosis. Materials and methods: Carrageenans of ι-, κ- and λ-types were incubated with washed erythrocytes (hematocrit 0.4%) at 0-1-5-10 g/L for either 24 h or 48 h. Incubation was follow...
Article
The development of inexpensive equipment adapted for the study of a specific biological object is very important for cryobiology. In the presented work, we have proposed a simple system for microscopy utilising open‐source platform Arduino. Testing this system showed that it had sufficient sensitivity to determine the physical processes occurring i...
Article
Full-text available
Purpose of Review A growing number of studies indicate that a network of regulated cell death (RCD) pathways plays a vital role in tumorigenesis suggesting its targeting to be a promising therapeutic avenue for cancer treatment. In this review, we firstly systematically summarize the current knowledge on the impact of carrageenans on different non-...
Article
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Search for new antimicrobial agents is of great significance due to the issue of antimicrobial resistance, which nowadays has become more important than many diseases. The aim of this study was to evaluate the toxicity and biological effects of a dextran-graft-polyacrylamide (D-PAA) polymer-nanocarrier with/without silver or gold nanoparticles (AgN...
Article
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Introduction Cancer chemotherapy faces two major challenges – high toxicity of active substances and tumor resistance to drugs. Low toxic nanocarriers in combination with anticancer agents can significantly increase the effectiveness of therapy. Modern advances in nanotechnology make it easy to create materials with the necessary physical and chemi...
Article
Aim. To analyze the eryptosis degree, the state of cell membranes and redox status of circulating red blood cells and leukocytes in patients with abnormal uterine bleeding and its combination with hypothyroidism. Materials and methods. Patients, 74 women aged 18 to 49 years, were examined, which were divided into 3 groups: group I - women with abno...
Article
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Introduction. Rare-earth orthovanadate nanoparticles (ReVO4:Eu3+, Re = Gd, Y or La) are promising agents for photodynamic therapy of cancer due to their modifiable redox properties. However, their toxicity limits their application.Objective. The aim of this research was to elucidate pro-eryptotic effects of GdVO4:Eu3+and LaVO4:Eu3+nanoparticles wit...
Article
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The aim of the present research was to assess the cytotoxicity of gold and silver nanoparticles synthesized into dextran-graft-polyacrylamide (D-PAA) polymer nanocarrier, which were used as a basis for further preparation of multicomponent nanocomposites revealed high efficacy for antitumor therapy. The evaluation of the influence of Me-polymer sys...
Article
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The search for novel antimicrobial agents is of huge importance. Nanomaterials can come to the rescue in this case. The aim of this study was to assess the cytotoxicity and antimicrobial effects of rare-earth-based orthovanadate nanoparticles. The cytotoxicity against host cells and antimicrobial activity of LaVO 4 :Eu 3+ and GdVO 4 :Eu 3+ nanopart...
Article
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Titanium dioxide (TiO2) nanoparticles are promising biomedical agents characterized by good biocompatibility. In this study, we explored the cytotoxicity of TiO2−x nanoparticles with a different Ti³⁺(Ti²⁺)/Ti⁴⁺ ratio and analyzed the efficiency of eryptosis indices as a tool in nanotoxicology. Two types of TiO2−x nanoparticles (NPs) were synthesize...
Preprint
Full-text available
Titanium dioxide (TiO2) nanoparticles are promising biomedical agents characterized by good biocompatibility. In this study, we explored the cytotoxicity of TiO2-x nanoparticles with a different Ti³⁺(Ti²⁺)/Ti⁴⁺ ratio and analyzed the effeciency of eryptosis indices as a tool in nanotoxicology. Methods. Two types of TiO2-x nanoparticles (5 nm) with...
Article
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Nanoparticles (NPs) have been reported to be promising enhancement agents for radiation therapy. The aim of the study was to assess the cytotoxicity of UV non-treated and UV pretreated GdYVO4:Eu3+ nanoparticles against erythrocytes and leukocytes by detecting eryptosis and reactive oxygen species (ROS) generation. Levels of intracellular ROS in ery...
Article
The purpose of the study was to conduct a comparative analysis of morphological examination of the placenta depending on the type of diabetes in pregnant women with placental dysfunction. Materials and methods. To analyze the morphological changes of the placenta in pregnant women with various forms of diabetes, 50 placentas were studied. The age o...
Article
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Background: Semi-refined carrageenan (food additive E407a) is a widely used thickener, which has been reported to exert toxic and pro-inflammatory effects. In particular, there is accumulating evidence that it induces eryptosis, i.e. a programmed cell death of eryptocytes, via ROS-mediated pathways. However, the role of Ca2+-dependent mechanisms in...
Article
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Aim: To assess the effects of semi-refined carrageenan (E407a) on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation in peripheral blood mononuclear cells (PBMCs) and LPS-mediated cell membrane alterations in leukocytes. Methods: Blood samples collected from 8 intact rats were incubated with E407a (10 mg/ml), E407a (50 mg/ml)...
Article
Full-text available
Oxidative stress caused by an overproduction of reactive oxygen species (ROS) is the key factor in developing a variety of pathological conditions. Recently various nanomaterials have attracted growing interest as nanoantioxidants with ROS-regulating ability. Here, for the first time, we report on high antioxidant behavior (enzyme-like activity) of...
Article
Full-text available
im. To evaluate the effects of semi-refined carrageenan (E407a) on the viability, metabolic and functional activity of skin fibroblasts in vitro. Materials and methods. Various concentrations of E407a (0 – 10 mg/ml) were incubated for 24 h with skin fibroblast cultures isolated from rat embryos. The viability of cells exposed to semi-refined carrag...
Article
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The safety of food additives E407 and E407a has raised concerns in the scientific community. Thus, this study aims to assess the local and systemic toxic effects of the common food additive E407a in rats orally exposed to it for two weeks. Complex evaluations of the effects of semi-refined carrageenan (E407a) on rats upon oral exposure were perform...
Article
Full-text available
Objectives. To study the effects of various concentrations of the food additive E407a (semi-refined carrageenan) on the metabolic activity of fetal liver cells, splenocytes, and bone marrow cells. Material and methods. Fetal liver, splenocytes and bone marrow cell cultures were incubated with the food additive E407a at concentrations varying from 0...
Article
Background: Concerns about the biosafety of the common food additive E407a have been raised. It has been demonstrated to induce intestinal inflammation, accompanied by activation of apoptosis, upon oral exposure. Thus, it is of interest to investigate how E407a affects eryptosis, a suicidal cell death mode of red blood cells. Objective: To evalu...
Article
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High efficacy of placental cells application necessitates their investigation. Preclinical studies require an improvement of the methods for obtaining, standardizing and storage of placental cells of experimental animals. Cells were isolated from rats and mice placentas by means of different enzymatic methods and the one of explants. Cells were cry...
Article
Gadolinium orthovanadate GdVO4:Eu 3+ nanoparticles (VNPs) have been shown to scavenge reactive oxygen species (ROS), making them a promising therapeutic agent in inflammation. This study aims to assess the effects of VNPs administered orally on E407a-induced inflammation. Materials and Methods: Fragments of the small intestine of 8 rats treated ora...
Article
Introduction. Hyaluronic acid is widely used in modern cosmetology, gynecology, arthrology, surgery and immunology. Warton's jelly (umbilical cord) was primary source of hyaluronate. At the same time, the effectiveness of stem cells, isolated from the Warton's jelly has been proven. This effect can be also realized through extracts, conditioned med...
Conference Paper
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Зниження статевої та репродуктивної функції чоловіків значно погіршує якість життя, соціальну адаптацію літніх чоловіків та чоловіків, які пройшли хіміотерапію. Важливим аспектом є можливість збереження сперміїв цих пацієнтів для використання в репродуктивних технологіях. Вивчення впливу різних хіміотерапевтичних засобів на статеву функцію та харак...
Conference Paper
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Клітинно-тканинна терапія на сьогоднішній день є одним з найперспективніших напрямків регенеративної медицини. Похідні плаценти, як біологічні структури, що характеризуються здатністю до синтезу багатьох регуляторних факторів та біологічно активних речовин, є перспективними об’єктами як для експериментальних досліджень, так і для клінічного застосу...
Article
Full-text available
Background. The human placenta is a promising source of biomaterial for regenerative medicine. This is primarily due to the availability of a sufficient amount of material, low immunogenicity, a large number of stem cells, and high proliferative cell potential. The effectiveness of stem cells, their lysates, and conditioned media in various patholo...
Conference Paper
Full-text available
Introduction. The male sexual and reproductive function decreasing significantly degrades the life quality, social adaptation of older men and men who underwent chemotherapy. The study of various chemotherapeutic agents effects on male sexual function will allow both to more accurately model diseases in the experiment and to individualize patients`...
Conference Paper
Full-text available
Introduction. The high clinical efficacy of cryopreserved placental derivatives in the treatment of certain diseases necessitates further preclinical studies to expand the indications for their use, which requires the availability of cryopreserved placental biomaterial. Aim. To study of cryoprotective effect of penetrating and non-penetrating cryop...
Poster
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https://cryo2020.com/about/ https://youtu.be/xx2p31inX_I
Conference Paper
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Вступ. Препарати гіалуронової кислоти широко застосовуються в сучас- ній косметології, гінекології, артрологїі, хірургії та імунології через їхні уніка- льні властивості [2, 3, 4, 7]. Первинним джерелом гіалуронату були тваринні компоненти, у тому числі пуповина, потім його стали отримувати шляхом син- тезу генетично модифікованими мікроорганізмами...
Presentation
Full-text available
Презентация к докладу на конференции. «Научный подход к сфере практической косметологии: актуальные вопросы и тренды»
Conference Paper
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Вступ. Реабілітація жінок після лікування онкопатології є актуальним питанням сучасної медицини. Зниження якості життя жінок відбувається значним чином за рахунок зниження функції яєчників після хіміотерапії. Перспективним методом відновлення статевої функції є застосування мезенхімальних стовбурових клітин (МСК) та похідних плаценти. Метою роботи...
Conference Paper
Full-text available
Вступ. Клітинне та органотипове культивування активно застосовується для медичних, біологічних та фармакологічних досліджень з 60-х років двадця- того сторіччя. Автоматизація виробництва обладнання та матеріалів робить ці методи все більш доступними для більшості лабораторій. Перевагою культура- льних методів є можливість визначення впливу тієї чи...
Conference Paper
Full-text available
Introduction. Hepatotoxicity is a severe complication of chemotherapy, which reduces the quality of life of the patients. Rehabilitation after cancer treatment remains an ur- gent task of modern medicine. Cells and other placental de- rivatives are promising candidates for treatment of a number of dystrophic diseases and can be used in treatment of...
Article
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Mesenchymal stem cells (MSCs) are actively used in regenerative medicine to treat neuropa- thies, diabetes mellitus, burns, ulcers, infertility, premature ovarian failure and climacteric syn- drome. MSCs application on different carriers allows to modify their properties for the different pathology treatment. For the biomaterials medical applicatio...
Article
Full-text available
Pelvic inflammatory disease is the most common gynecological pathology. A number of studies have demonstrated the effectiveness of placenta derivatives for the female genital system pathology treatment. The aim of the study was to determine the effect of cryopreserved placenta explants on ovaries and uterus recovery after an infectious process. M...
Conference Paper
Full-text available
Вступ. Актуальною проблемою гемотрансфузіології є збільшення по- треб у свіжозамороженій плазмі зі збереженням її складових, особливо факторів згортання. Біологічно активні сполуки забезпечують високий рівень функціонування організму дитини, тому розробка програм кріо- консервування плазми плацентарної крові (ППК) дозволить розширити арсенал гемотр...
Article
Full-text available
Background. Placental mesenchymal stem cells application is a promising area of cell therapy for number diseases. Their using in the form of spheroids results in enhancement of the therapeutic potential, improve the cells survival after transplantation. Medical purposes require the characterization and development of spheroids storage technology. O...
Article
Full-text available
Клітини, тканини, біологічно активні речовини плаценти є перспективними об’єктами для ефективного лікування патологічних станів ендокринної, імунної та нервової систем. Для клінічного застосування похідних плаценти необхідна розробка методів їх короткотривалого зберігання і транспортування від клініки до біотехнологічної лабораторії та в зворотному...