Volodymyr HalytskiyNational Academy of Sciences of Ukraine | ISP · Department of Molecular Immunology
Volodymyr Halytskiy
MD, PhD (biological sciences - biochemistry)
About
96
Publications
2,217
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
60
Citations
Introduction
Volodymyr Halytskiy currently works at the Department of Molecular Immunology, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. Volodymyr does research in Molecular Oncology, Immunooncology, non-coding RNAomics and Epigenetics. Their current project is 'Theory of epigenetic carcinogenesis.'
Additional affiliations
January 2014 - present
November 2006 - December 2013
Education
November 2003 - October 2006
Publications
Publications (96)
Non-coding RNAs are widespread class of cell RNAs. They participate in many important processes in cells - signaling, posttranscriptional silencing, protein biosynthesis, splicing, maintenance of genome stability, telomere lengthening, X-inactivation. Nevertheless, activity of these RNAs is not restricted to posttranscriptional sphere, but cover al...
The idea proposed in this article is that a specific set of microRNAs expressing in stem cells can restore the initial profile of their epigenetic markers through RNAi-directed DNA methylation, and owing to that the pluripotent immortal status of these cells is supported unlimitedly and possibly minimum level of the mobile genomic elements activity...
We have discovered that 5'-CG-3' dinucleotide and 5'-CNG-3' trinucleotide are found in published sequences of small interfering RNA and microRNA more often than they should be found in a random sequence. This circumstance is evidence of an important biological purpose of 5'-CG-3' dinucleotides and 5'-CNG-3' trinucleotides in small RNA sequences. We...
Gene network of the lymphoid cell differentiation coordinates precisely the recombination process in immunoglobulin gene loci. In our opinion, cellular microRNAs can contribute to the allelic exclusion through microRNA-directed DNA methylation and participate in retargeting recombinases activity from the gene loci of heavy immunoglobulin chains to...
Lymphoid cells differentiation is a well coordinated and highly integrated process, which is defined by the interaction of genes and cell signaling pathways of the maturing cell with the microenvironment factors. In the course of such interaction in the maturing cell, new ways of signal transduction appear, and on the basis of such new ways new gen...
Background:
Dysregulated miRNA expression has been found in nearly all the stages of cancer process compared to normal tissue. Certain miRNAs expression level is closely related to the histopathological parameters and molecular subtypes of breast cancer (BC) as well as the response to treatment and prognosis.
Methods:
This prospective research has...
Background
Doxorubicin is an anthracycline antibiotic that acts as a DNA intercalating agent, inhibiting the topoisomerase II and inducing the apoptotic cell death, mainly due to the accumulation of double-strand DNA breaks (DSB). This research aims to identify in what way the abnormalities in microRNA (miRNA) expression profile in cancer cells can...
Background: To attack tumors, cytotoxic CD8+ T cells need to recognize tumor antigen peptides loaded onto major histocompatibility complex (MHC) class I molecules. Antigen processing and presentation is multistep process that includes proteasome-dependent and independent cleavage of cytosolic proteins, transport of derived peptides into the endopla...
The current paradigm considers carcinogenesis to be a result of mutation, following activation (initiation and promotion, respectively) of cell oncogenes. However, oncogene abnormalities are not always revealed in tumor cells. At the same time, tumor growth is associated with widespread shifts in non-coding RNAome, esp. in microRNA (miRNA) pattern....
Background: One of the main immune escape mechanisms used by tumors is the exploitation of immune checkpoints – receptors on surface of immune cells, which, after binding their ligands, can suppress immune response. This research aims to identify in what way the shifts in miRNA expression pattern can contribute to the expression of immune checkpoin...
Background: One of the main immune escape mechanisms used by tumors is the exploitation of immune checkpoints - receptors on surface of immune cells, which, after binding their ligands, can suppress immune response. Consequently, immune checkpoint blockade is considered as promising approach for cancer treatment. However, currently available anti-C...
Background: One of the crucial causes of the multiple drug resistance (MDR) is overexpression of genes encoding the ATP binding cassette (ABC) transporters that mediate effective efflux of anticancer agents. MDR phenomenon exemplifies exceptional plasticity and adaptability of tumors. This research aims to identify in what way the tumor-related shi...
ADAR (adenosine deaminase acting on RNA)-mediated RNA editing takes place in many cancer types and correlates with tumor progression, invasion potential, therapeutic resistance and poor prognosis. Our research aims to identify in what way the shifts in miRNA expression profile can facilitate ADAR expression and contribute thereby to RNA editing abn...
Aims: Anoikis is a specific form of apoptosis induced by disengagement of integrins from extracellular matrix in case of cell detachment. For successful migration and metastasis, tumor cells must avoid anoikis and acquire anchorage-independency. This research aims to identify in what way the abnormalities in expression of non-coding RNAs, especiall...
Background: Epithelial-mesenchymal transition (EMT) is transdifferentiation process in which epithelial cells downregulate epithelial gene expression signature, lose cell polarity, junctions with each other as well as interactions with basement membrane and acquire mesenchymal traits, incl. stem cell properties, enhanced motility, invasiveness and...
Genome instability, high mutation rate as well as epigenetic dysregulation are typical features of many cancer cells leading to their high variability and, as a result, to the tumour evolution. Our investigation aims to identify in what way the shifts in miRNAome can contribute to the DNA damage. MiRNA targets within gene transcripts were predicted...
Overall, macrophages and some subtypes of lymphoid cells are found in tumour stroma. These cells secrete a variety of growth factors, proinflammatory cytokines and chemokines, esp. TNF-a, IL-1b and IL-6, causing the formation of inflammatory microenvironment around tumour cells. TNF-a and IL-1b signaling increases activity of NF-kB pathway. At the...
Background: Genome instability, high mutation rate as well as high variability is typical of many cancer cells. Also, tumor growth is tightly associated with regular shifts in miRNA expression pattern. Usually, expression of miRNAs miR-18a/b, miR-19, miR-21, miR-23a/b, miR-29a, miR-155, miR-181, miR-206, miR-210, miR-221/222 and miR-375 is up-regul...
Introduction:
Impaired balance between DNA methylation and demethylation, deregulation of chromatin remodeling, genomic instability and activity of transposable elements (TEs) become apparent in aging cells and are typical of cancer cells. This research aims to identify in what way the shifts in expression of non-coding RNAs, esp. miRNAs, can lead...
Aims: Gastric MALToma (B-cell lymphoma of the mucosa-associated lymphoid tissue) is the unique malignancy which survival and growth, at least at low-grade stages, are determined by permanent presence of Helicobacter pylori as well as by chronic inflammation caused by this pathogen. Inflammatory microenvironment cells, esp. T-cells, present a variet...
Background: Impaired adhesion, loss of cell polarity and aberrant orientation of mitotic spindle are typical for cancer cells. Moreover, alterations in cell polarity and in normal asymmetry of stem cell division may also contribute to the early stages of carcinogenesis. This research aims to identify in what way the shifts in expression of non-codi...
Background: Loss of contact inhibition is one of the most prominent features of transformed cells, resulting in uncontrolled cell proliferation and movement. This research aims to identify in what way the shifts in expression of non-coding RNAs, especially miRNAs, can affect the signal pathways responsible for contact inhibition.
Material and metho...
Background: Overall, macrophages and some subtypes of lymphoid cells are found in tumour stroma. These cells secrete a variety of growth factors, proinflammatory cytokines and chemokines, esp. TNF-a and IL-1b, causing the formation of inflammatory microenvironment around tumour cells. TNF-a and IL-1b signaling increases activity of NF-kB pathway du...
Our recent studies found that miRNAs, hyperexpression (or reactivation) of which is essential for cancer cells, silence: 1) genes encoding DNA repair enzymes as well as other key elements of all DNA repair systems; 2) genes of histone deacetylases, histone methyltransferases and de novo DNA methyltransferases; 3) genes encoding cytoskeleton and nuc...
Explanation of allelic exclusion as well as other gene dosage effects is a very complicated problem, because they can be established in spite of largest fluctuations of expression level and regulatory influences. Nevertheless, the molecular mechanism of allelic exclusion should be surprisingly simple when supposing that the non-template DNA strand...
Introduction: Loss of cell polarity, contact inhibition breakage, aberrant orientation of mitotic spindle and adhesion abnormalities are typical for cancer cells. Moreover, alterations in cell polarity and in normal asymmetry of stem cell division may also contribute to the early stages of carcinogenesis. Our investigation aims to identify in what...
Aberrant nuclear morphology and impaired nuclear transport are typical for tumor cells. Our investigation aims to identify in what way shifts in expression of non-coding RNA, especially microRNA, can contribute to these abnormalities.
MicroRNA targets within gene transcripts were predicted in silico using TargetScan software.
We found that transcri...
According to our recent studies, non-coding RNAs, hyperexpression (or reactivation) of which is essential for cancer cells, silence:
1) genes encoding DNA repair enzymes and other key elements of DNA repair networks;
2) genes of histone deacetylases, histone methyltransferases and de novo DNA methyltransferases;
3) genes encoding nuclear lamina pro...
Goals: Genome instability, high mutation rate as well as high variability is typical of many cancer cells. Also, tumor growth is tightly associated with regular shifts in miRNA expression pattern. Usually, expression of miRNAs miR-18a/b, miR-19, miR-21, miR-23a/b, miR-29a, miR-155, miR-181, miR-206, miR-210, miR-221/222 and miR-375 is up-regulated...
Our recent studies found that non-coding RNAs, hyperexpression (or reactivation) of which is essential for cancer cells, silence: 1) genes encoding DNA repair enzymes as well as other key elements of all DNA repair systems; 2) genes of histone deacetylases, histone methyltransferases and de novo DNA methyltransferases [1]; 3) genes encoding nuclear...
Background: Tumor growth is tightly associated with regular shifts in microRNA (miRNA) expression pattern. Usually, expression of miRNAs miR-15/16, miR-128, miR-145, miR-150, miR-148/152 and miR-299 is down-regulated in cancer cells whereas expression of miRNAs miR-19, miR-23, miR-27, miR-155 and miR-223 is up-regulated. This investigation aims to...
In germline and in some somatic cells during early embryogenesis, repression of transposable elements (TEs) is performed via de novo RNA-directed DNA methylation within TE sequences bound with piRNAs and endo-siRNAs. However, after the early embryogenesis the expression of piRNAs and endo-siRNAs is lost. After that, TE reactivation is prevented mai...
Background: Tumor growth is associated with regular shifts in microRNA (miRNA) expression pattern. Usually, expression of miRNAs miR-15/16, miR-128, miR-145, miR-150, miR-148/152 and miR-299 is down-regulated in cancer cells whereas expression of miRNAs miR-19, miR-23, miR-27, miR-155 and miR-223 is up-regulated. This investigation aims to identify...
Part of mobile genetic elements (MGEs) in human genome is 45%, which corresponds to 3-4 million copies. MGEs are the genomic parasites that pose a serious threat as they can cause both the genome destabilization through overall lesions and single mutations, including mutations that provoke tumor growth as well as other diseases. In germline and som...
Non-coding RNAs are widespread class of cell RNAs. They participate in many important processes in cells – signaling, posttranscriptional silencing, protein biosynthesis, splicing, maintenance of genome stability, telomere lengthening, X-inactivation. Nevertheless, activity of these RNAs is not restricted to posttranscriptional sphere, but cover al...
Although the concentration of CG and CNG sites in Xist RNA averages 0.8%, that is even less than the general genome level (1%), the localization of these sites reveals extreme irregularity. CG and CNG concentration reaches maximum density, exceeding the average level several times, at the 5’ end of the Xist transcript. Frequency of CG and CNG sites...
Cell miRNA network is intertwined with signal transduction pathways. Cancer cells down-regulate expression of miRNAs that silence proliferative and antiapoptotic genes and thus can prevent from abnormal cell proliferation and surviving. Up-regulated miRNAs suppress genes encoding cell cycle inhibitors as well as genes responsible for cell different...
Background:
Although inflammation is closely associated with tumor growth, molecular basis of this interrelation remains unclear, especially when pathogens do not damage the DNA. However, the inflammation entails regular changes in the expression of cell microRNAs (miRNAs). Expression of miRNAs miR-155, miR-21, miR-146a, miR-125b, miR-31, miR-34c,...
Although the concentration of CG and CNG sites in Xist averages 0.8%, that is even less than the general genome level (1%), the localization of these sites reveals extreme irregularity. Of particular interest are: 1) area that includes the first 2000 nucleotides from 5’-end of the Xist RNA (within its first exon) and 2) small A region within this a...
We have found in silico that the concentration of CG dinucleotide reaches 2.85% in siRNA sequences and 2.58% in mature miRNA sequences. It was quite intriguing that the CG frequency in miRNAs and siRNAs significantly exceeds the general genome level (1%). The concentration of CNG trinucleotides amounts to 6.29% in siRNA sequences and to 5.77% in ma...
Background: Epigenetic instability, deregulation of chromatin remodeling as well as impaired balance between DNA methylation and demethylation is typical of many cancer cells. Also, tumor growth is tightly associated with regular shifts in microRNA (miRNA) expression pattern. Usually, expression of miRNAs miR-16, miR-122, miR-31, miR-143, miR-145 a...
Background: After the rearrangement in immunoglobulin heavy locus (IgH) in proB cells, recombinase genes Rag1 and Rag2 undergo temporary repression. Their expression is renewed again in preBII cells. Simultaneously, non-rearranged allelic IgH is repressed and immunoglobulin light chain locus (IgL) undergoes derepression. As a result, recombinase ac...
Background: Although inflammation is associated with tumor growth, molecular basis of this phenomenon remains unclear, especially when pathogens do not have the ability to damage DNA immediately. However, the inflammation entails regular changes in the expression of cell microRNAs (miRNAs). Expression of miRNAs miR-155, miR-21, miR-146a, miR-125b,...
Objectives: Having found in silico that the density of CG and CNG sites in short non-coding RNAs – miRNAs and siRNAs – greatly exceeds the average genome level, I have suggested that these RNAs can directly hybridize with target DNA sequences, thereafter involving DNA methyltransferases in methylation de novo of cytosine within DNA targets [1]. The...
Goals:
Tumor growth is tightly associated with regular shifts in microRNA (miRNA) expression pattern. Usually, expression of miRNAs miR-16, miR-122, miR-125a/b, miR-31, miR-143, miR-145, miR-204, miR-205 and miR-320 is down-regulated in breast cancer cells whereas expression of miRNAs miR-18a/b, miR-19, miR-21, miR-29a, miR-155, miR-181, miR-206, m...
Tumor growth is associated with regular shifts in miRNA expression pattern. Cancer cells down-regulate expression of miRNAs miR-15a/16, miR-17-5p, miR-31, miR-125a/b, miR-143 and miR-145 that silence proliferative and antiapoptotic genes [1]. miRNA miR-17-5p can target transcripts of genes coding proliferative signal pathway components E2F1/2, STAT...
Goals:
Tumor growth is tightly associated with regular shifts in microRNA (miRNA) expression pattern. More than 50% of miRNA genes are located in fragile chromosomal regions that are susceptible to various damages during the carcinogenesis. Usually, expression of miRNAs miR-22, miR-29, miR-122a, miR-124, miR-126, miR-148, miR-152, miR-203, miR-223,...
Normal cells can undergo no more than a certain number of divisions. This phenomenon was usually explained by the assumption that proliferation of cells lacking the telomerase is possible until their telomeres reach a critical minimum length through the end-replication problem. However, the telomere length does not correlate with lifespan of differ...
Background: Tumor growth is tightly associated with regular shifts in microRNA (miRNA) expression pattern. More than 50% of miRNA genes are located in fragile chromosomal regions that are susceptible to amplification, translocation and other damages during the carcinogenesis. Usually, expression of miRNAs miR-16, miR-122, miR-125a/b, miR-31, miR-14...
Aim. Discovery of localization pattern of 5'-CG-3' dinucleotide and 5'-CNG-3' trinucleotide in microRNA (miRNA) sequences. Methods. Bioinformatic and statistic analysis of mature miRNA sequences. Results. One of the highest peaks of 5'-CG-3' dinucleo-tides concentration is located close to the nucleotide 10 from 5'-end of miRNA. The level of 5'-CG-...
Background: Tumor growth is tightly associated with regular shifts in microRNA (miRNA) expression pattern. Expression of several miRNAs, e.g. miR-21, miR-23a/b, miR-100, miR-146a, miR-155, miR-181, miR-206, miR-221 and miR-222, is up-regulated in leukemia cells. This investigation aims to identify how abnormalities in miRNA network contribute to th...
Background: Tumour growth is tightly associated with regular shifts in microRNA (miRNA) expression pattern as well as with amplification, translocation and other damages in fragile chromosome regions containing more than 50% of miRNA genes. Usually, expression of miRNAs miR-15a, miR-16, miR-17-5p, miR-31, miR-125a/b, miR-143 and miR-145 is down-reg...
According to hypothesis of microRNA-directed DNA methylation, some microRNAs (miRNAs) are involved in allelic exclusion [1]. The precursors of these miRNAs should be transcribed from sequences that undergo allelic exclusion. Mature miRNAs can directly hybridize with complementary DNA untwisted during the transcription, thereafter involving DNA meth...
Goals: More than 50% of microRNA (miRNA) genes are located in fragile chromosomal regions that are susceptible to amplification, translocation and other damages during the carcinogenesis. This investigation aims to identify why tumour growth needs abnormalities in the set of cell miRNAs as well as deregulation of which miRNA genes contributes to th...
Expression of transposons can be suppressed by methylation of their DNA. This suppression depends on some classes of interfering RNAs (RNAi) such as PIWI-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs) and microRNAs (miRNAs). Having found in silico that the frequency of CG and CNG sites in these non-coding RNAs greatly ex...
Transposable elements (TEs) are repressed in cells through DNA methylation during the gametogenesis and early embryogenesis. This process depends on some classes of small RNA such as PIWI-interacting RNA (piRNA), endogenous small interfering RNA (endo-siRNA) and microRNA (miRNA). We discovered recently that CG and CNG sites are found in cellular sm...
CG and CNG sites are found recently in microRNA (miRNA) sequences more often than they should be found in random DNA sequence. Therefore we hypothesized that binding of complementary DNA sequence by miRNA leads to recruiting of DNA methyltransferases that methylate de novo the DNA target. As miRNA genes are the transposable element derivates, we su...
We discovered recently that 5’-CG-3’ and 5’-CNG-3’ sites are found in interfering RNAs (RNAi) sequences more often than they should be found in random DNA sequence. Therefore we hypothesized that binding of complementary DNA sequence by RNAi leads to recruiting of DNA methyltransferases that methylate de novo cytosine in 5’-CG-3’ and 5’-CNG-3’ site...
Apparently, a specific set of microRNAs and piRNAs expressing in stem cells can restore initial profile of their epigenetic markers through RNAi-directed DNA methylation, thus pluripotent immortal status of these cells is supported for ever and minimal level of the mobile genomic elements activity is achieved. However, cell differentiation, startin...
We discovered recently that 5’-CG-3’ dinucleotide and 5’-CNG-3’ trinucleotide are found in sequences of small interfering RNA (siRNA) and microRNA (miRNA) more often than they should be found in random DNA sequence. In our opinion, recognition and binding of complementary sequence in DNA by miRNA and siRNA leads to recruiting of DNA methyltransfera...
Apparently, a specific set of microRNAs and piRNAs expressing in the stem cells can restore initial profile of their epigenetic markers through the RNAi-directed DNA methylation, thus pluripotent immortal status of these cells is supported for ever and minimal level of the mobile genomic elements activity is achieved. However, cell differentiation,...
We hypothesize that a specific set of microRNAs expressing in embryonic stem cells supports pluripotent immortal status of these cells by active renewing of proper profile of the epigenetic markers through microRNA-directed DNA methylation even in case of demethylation of both DNA chains. In this way minimal possible level of the mobile genomic ele...
In our opinion, gene module – a group of together expressed genes, which are specific only for the certain differentiation stage and execute program of this stage - is the elementary unit of cell differentiation network. Making choice on direction of the next differentiation stage takes place after completion of the previous one. Cell receptor join...
We examined microRNA (miRNA) sequences in order to identify possible mechanism of sequence-specific targeting of genes during the transcriptional silencing. Human, mouse and rat sequences of mature miRNA - in all 1083 sequences - were extracted from database miRBase, http://microrna.sanger.ac.uk/. We detected that only 20.96% miRNA molecules did no...
Initial biological sense of apoptosis, іn our opinion, consisted in ensuring most quick release of endosymbionts, afterwards transformed into mitochondria, from symbiosis, provided the probability for irreversible damage and following death of host cell was very high. Note, that apoptosis signal network resembles the latent phage activation network...
The cell differentiation is a result of a precisely coordinated activity of cell genes united by intracellular signal transduction cascades into gene network. In our opinion, elementary unit of this network is the gene module - group of together expressed genes, which are specific only for certain stage of cell differentiation and execute program o...
We examined microRNA sequences in order to identify possible mechanism of sequence-specific targeting of genes during the transcriptional silencing. Human, mouse and rat sequences of mature microRNAs - in all 1083 sequences - were extracted from database miRBase, http://microrna.sanger.ac.uk/. We detected that only 20.96% microRNA molecules did not...
We have established that 5′-CG-3′ dinucleotide and 5′-CNG-3′ trinucleotide are found in published sequences of small interfering RNA and microRNA more often than they should be in random DNA sequences. This circumstance indicates the important biological role played by 5′-CG-3′ dinucleotides and 5′-CNG-3′ trinucleotides in small RNA sequences. We s...
DNA methylation is an important epigenetic mechanism that assigns and maintains the gene expression profile and enables cell differentiation, allelic exclusion and other key phenomena. We investigate possible role of small interfering RNA and microRNA in the DNA methylation de novo.
Human, mouse and rat sequences of siRNA – in all 599 sequences – w...
Human, mouse and rat sequences of small interfering RNA (siRNA) – in all 599 sequences – were extracted from database “siRNA Database and Resources for RNA Interference Studies”, http://www.rnainterference.org/Sequences.html. Human, mouse and rat sequences of mature microRNA (miRNA) – in all 1083 equences – were extracted from database miRBase, htt...
Background:
In our opinion, separate module of the gene network of cell differentiation is a gene cluster - group of together expressed genes, which are specific only for certain stage of cell differentiation. Individual gene cluster is composed of genes of two classes: a) gene-activator; its protein supports activity of the own gene (positive feed...
Cell differentiation is a result of the exactly coordinated activity of cell genes united by signal transduction cascades into the gene network. In our opinion, separate module of this network is a gene cluster – group of together expressed genes, which are specific only for certain stage of cell differentiation. Individual gene cluster is composed...
We suggested a cluster idea of the gene network structure to provide a framework for the analysis of cell differentiation. In our opinion, cell differentiation is a result of exact coordinated activity of cellular genes united by intracellular signal transduction cascades into gene network. Separate module of this network is the genes cluster – gro...
We suppose that each stage of every direction of cell differentiation is executed by gene cluster – separate gene network that is specific only to this stage. Individual gene cluster is constituted by genes of four classes:
a) gene-activator; its protein (transcription factor) maintains activity of proper gene as well as all other genes of this clu...
The paper considers age-associated alterations of intracellular and intercellular cascades of transduction of proliferative, differentiating, pro- and antiapoptotic signals, their interaction and influence on proliferative activity, differentiation and apoptosis of the immune system cells. One of initial causes of these alterations is accumulation...
The unified conception of the origin of eukaryotic cells has been proposed. In the author's opinion, evolutionary transformation of prokaryotic cell into eukaryotic cell took place 3.3-1.4 billion years ago and involved the next four stages: 1) the appearance of intracellular membranes due to prokaryotic cell plasmalemma invaginating into its cytop...
In our opinion, tumour arises when transformed cells do not receive growth factors (GFs) required for their subsequent differentiation. As a result, the process of differentiation of mentioned cells is suspended at certain level. But these cells proliferate uninterruptedly and avoid apoptosis menace, because the oncoproteins of changed, permanently...
Mitochondria, as is known, are essential for Bcl-2/p53-mediated apoptosis of multicellular organisms cells, that is no coincidence. In our opinion, apoptosis is a reflection of symbiotic interrelations between nuclear cells and microorganisms afterwards transformed into mitochondria; it is an evolutionary altered reaction of endosymbionts to the in...
В этой работе подытожены данные современных исследований в области молекулярных механизмов внутриклеточной передачи сигналов, с учётом чего рассмотрены закономерности возникновения в клетке несанкционированных сигналов. Обращено внимание на особую схему организации и тесную взаимосвязь каскадов, передающих внутриклеточные сигналы, с некоторыми про-...
In research sumed up the data of modern investigations in sphere of molecular mechanisms of intracellular signals transduction, considering of it was examined objective laws of appearance in cell of unsanctioned signals. Pointed attention for especial scheme of organization and tough communication of cascades, which transduct intracellular signals,...
As is known, risk of tumour appearance increases substantially with atrophy processes in organism’s tissues. Such phenomenon, in our opinion, is quite natural and stipulated by two following causes.
Firstly, death of considerable quantity of cells leads to compensative acceleration of proliferation (and it should be expected, that in situation, whe...
This paper formulates a conception of cluster organization of the genetic material, responsible for cell determination and differentiation in multicellular organisms. According to this conception, gene controlling each particular stage of these processes are functionally integrated via genes-activators into gene groups (clusters) of different level...
В работе сформулирована концепция кластерной организации генетического материала, ответственного за детерминацию и дифференцировку клеток многоклеточных организмов, согласно которой гены, контролирующие каждый определённый этап этих процессов, функционально объединены посредством генов-активаторов в генные группы (кластеры) разных уровней иерархии....
Projects
Project (1)
Current paradigm considers carcinogenesis as a result of mutation and following activation (initiation and promotion, respectively) of cell oncogenes. However, oncogene abnormalities are not always revealed in tumor cells, e.g. in case of low-grade MALToma. At the same time, tumor growth is associated with widespread shifts in non-coding RNAome, esp. in miRNAome. Current investigation aims to identify in what way the shifts in miRNAome can lead to the cell transformation. On this basis, we attempt to suggest a novel theory of epigenetic carcinogenesis.
