Vladimir Shmurak

Vladimir Shmurak
Sechenov Institute of Evolutionary Physiology and Biochemistry · LABORATORY of ENZYMES COMPARATIVE BIOCHEMISTRY

Bachelor of Technology

About

18
Publications
1,204
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174
Citations
Citations since 2017
14 Research Items
173 Citations
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20172018201920202021202220230102030405060
20172018201920202021202220230102030405060
20172018201920202021202220230102030405060

Publications

Publications (18)
Conference Paper
Full-text available
In the present study, preclinical testing of the effect of modulators of the ligand-binding and enzymatic properties of albumin on the survival of rats in acute poisoning with organophosphates (OPs) has been performed. According to the obtained data, the application of epigallocatechin gallate (EGCG) as a part of adjuvant therapy promoted the survi...
Article
Full-text available
Serum albumin possesses esterase and pseudo-esterase activities towards a number of endogenous and exogenous substrates, but the mechanism of interaction of various esters and other compounds with albumin is still unclear. In the present study, proton nuclear magnetic resonance (1H NMR) has been applied to the study of true esterase activity of alb...
Article
Full-text available
Being one of the main proteins in the human body and many animal species, albumin plays a decisive role in the transport of various ions—electrically neutral and charged molecules—and in maintaining the colloidal osmotic pressure of the blood. Albumin is able to bind to almost all known drugs, as well as many nutraceuticals and toxic substances, la...
Article
Full-text available
As a carrier of many biologically active compounds, blood is exposed to oxidants to a greater extent than the intracellular environment. Serum albumin plays a key role in antioxidant defence under both normal and oxidative stress conditions. This review evaluates data published in the literature and from our own research on the mechanisms of the en...
Article
Full-text available
The aim of the present work was to study the effects of transient and prolonged uncontrollable stress on the behavior of animals with different types of stress reactions and social status. Transient stress (forced swimming for 5 min) led to suppression of movement activity in subordinate animals with the inactive type of stress reaction, while only...
Article
Studying the drugs of natural origin that promote recovery of an organism after acute poisoning with household and industrial toxicants, including organophosphates (OPs), is a challenge not only to toxicology but also informatics, which is a major tool for developing the methodology of system analysis. We report here the data on the effect of decaf...
Article
The long-term effects of acute organophosphate (OP) poisoning remain poorly studied, while experimental models usually disregard species-related specificity of rodents as model objects. Here we present two toxicological models and a comparative analysis of a wide range of biochemical blood indices in their dynamics over 3 months after acute rat poi...
Article
Full-text available
The albumin molecule, in contrast to many other plasma proteins, is not covered with a carbohydrate moiety and can bind and transport various molecules of endogenous and exogenous origin. The enzymatic activity of albumin, the existence of which many scientists perceive skeptically, is much less studied. In toxicology, understanding the mechanistic...
Article
Enzymatic activities of three types of serum albumin—rat, bovine and human—were analyzed comparatively using a mathematical model. Kinetic and equilibrium constants of carboxylesterase and paraoxonase activities of albumin in Sudlow’s sites I and II were determined. The effects of specific ligands, ibuprofen and warfarin, on enzyme kinetics in thes...
Article
Albumin is known to be able to cleave ether bonds in organophosphates (OPs). Amino acids responsible for esterase and pseudo-esterase albumin activity towards OPs are not yet finally identified. Presumably, Sudlow’s site I with the Tyr150 residue shows a “true” esterase activity, while Sudlow’s II site with the Tyr411 residue—a pseudo-esterase one....
Article
Full-text available
A comparative investigation of the esterase profiles of blood of mice of both genders exposed to various doses of 2-(o-crezyl)-4H-1,3,2-benzodioxaphosphorin-2-oxide (CBDP) was made an hour after its percutaneous injection to animals. A lesser amount of esterase in males blood made them more susceptible to CBDP action as compared to females. It was...
Article
The article covers problems of biochemical methods assessing organophosphorus toxic compounds in objects of chemical weapons extinction. The authors present results of works developing new, more specific and selective biochemical methods.
Article
It is known that albumin is able to cut ester bonds in organophosphates (OPs). Amino acids responsible for esterase and pseudo-esterase activity of albumin towards OPs are still not determined. The purpose of this study is to identify the potential sites of esterase activity of albumin by the example of its interaction with soman using molecular mo...
Article
Full-text available
The paper presents the study of the excretion of sulfated glycosaminoglycans (GAG) in the urine of rats in experimental hemorrhagic cystitis induced by cyclophosphamide and treated with glycosaminoglycan replacement therapy. Rats were given intraperitoneal injections of cyclophosphamide at a dose of 100 mg per 1 kg body weight and subsequently trea...
Article
Albumin is known to be able to interact with organophosphorus compounds (OPCs), but neither amino acid residues of albumin that are responsible for this interaction nor the nature of the forming bonds have been finally established. Catalytic and pseudocatalytic functions of albumin are under consideration. Possible sites of interaction of albumin w...

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