Vladimir A. Kulchitsky

National Academy of Sciences of Belarus | NAS B · Institute of Physiology

This study investigates rational approaches to the targeted delivery of chemotherapeutic drugs, with a focus on cisplatin, and explores methods to enhance their cytotoxic effects. The work presents results from computer modeling of the structural and electronic characteristics of quinine, fullerene, and cisplatin. Using quantum-chemical modeling wi...

Alkaloid-based urea derivatives were produced with high yield through the reaction of anabasine and cytisine with isoxazolylphenylcarbamates in boiling benzene. Their antitumor activity, in combination with the commonly used five anticancer drugs, namely cyclophosphane, fluorouracil, etoposide, cisplatin, ribomustine with different mechanisms of ac...

Alkaloid-based urea derivatives were produced with high yield through the reaction of anabasine and cytisine with isoxazolylphenylcarbamates in boiling benzene. Their antitumor ac-tivity in combination with the commonly used six anticancer drugs namely cyclophosphane, fluorouracil, etoposide, cysplatine, ribomustine with different mechanisms of act...

Описан тетраэйкозагидроксифуллерен С60(OH)24: синтез, свойства и примеры применения

Представлены фармакофорные дискрипторы конъюгата карбоплатин-фуллеренол: DFT моделирование

Nitric oxide (NO) production in injured and intact brain regions was compared by EPR spectroscopy in a model of brain and spinal cord injury in Wistar rats. The precentral gyrus of the brain was injured, followed by the spinal cord at the level of the first lumbar vertebra. Seven days after brain injury, a reduction in NO content of 84% in injured...

Тетраэйкозагидроксифуллерен С 60 (ОН) 24 обладает широким спектром уникальных и цели-тельных свойств, позволяющих с успехом использовать его в целом ряде отраслей народного хозяйства. Внимание к этому соединению обусловлено его чрезвычайно высоким биологиче-ским потенциалом, проявляющимся в потенцирующим действии химиопрепаратов против злокачествен...

Here we performed a comparative experimental analysis by EPR spectroscopy of the intensity of nitric oxide (NO) production and copper content in the injured and uninjured areas of the frontal lobes and the hippocampus of the brain of male Wistar rats, after modeling of combined brain and spinal cord injury. Brain and spinal cord injury were modelle...

Chemotherapy is one of the main treatment options for cancer, but it is usually accompanied with negative side effects. The classical drugs combination with synergistic adjuvants can be the solution to this problem, allowing reducing therapeutic dose. Elucidating the mechanism of adjuvant action is of key importance for the selection of the optimal...

Описан синтез и противоопухолевая активность изоксазолилмочевин с фрагментами алкалоидов анабазина и цитизина

Biological Activity of Fullerenol – Carboplatin Conjugate Agent of Antitumor Therapy: Quantum Chemical Simulation are reported

Представлены результаты моделирования методом HF-3c конъюгата карбоплатин−фуллеренол как перспективной структуры для противоопухолевой терапии онкологических заболеваний

Hypoxia occurs in situations of disbalance between metabolic needs and the supply of oxygen to organs and tissues of the body. In this regard, tissue hypoxia and ischemia are essential components of the pathogenesis of many diseases. One of the promising areas of research into the mechanisms of ischemia is attempting to weaken the negative effect o...

In the era of increasing the effectiveness of treatment methods and drugs used in modern neuro-oncology, the targeted delivery of diagnostic and medicinal substances to the tumor is of great importance. The aim of the work is to study in silico optimal and rational approaches to the creation of nanocontainers for targeted drug delivery. Here we pre...

В работе приведены результаты моделирования методом PM7 атомной и электронной структуры водорастворимых производных конъюгатов карбоплатина и наноуглеродных структур, таких, как фуллеренол (C60(OH)24), а также результаты изучения механизмов цитостатической активности данных комплексов.

Представлено использование в качестве трейлеров структурных фрагментов молекул растительного происхождения (алкалоидов) в молекулярном дизайне новых потенциальных лекарственных средств.

The aim of this work is quantum-chemical simulation of the electronic structure and analysis of the thermodynamic stability of new doxorubicin-fullerenol agents for the treatment of tumor neoplasms. The need for preliminary studies of modeling such objects is due to the extremely high labor intensity, cost and complexity of their practical producti...

The aim of this work is to quantum-chemical simulation of the electronic structure and analysis of the thermodynamic stability of new doxorubicin-fullerenol agents for improving the effectiveness of malignant tumor therapy. The need for preliminary studies of modeling such objects is due to the extremely high labor intensity, cost and complexit...

The aim of this work is to substantiate a new methodology for the search for modern biologically active compounds and potential drugs by analyzing literature data on their activity and synthesizing analogues and homologues of recognized, patented drugs, for example, generics, their subsequent directed functionalization to identify the effect of che...

Hypoxia occurs in situations of disbalancebetween metabolic needs and the supply of oxygen to organs and tissues of the body. In this regard, tissue hypoxia and ischemia are essential components of the pathogenesis of many diseases. One of the promising areas of research into the mechanisms of ischemia is attempting to weaken the negative effect of...

The use of Stem Cells (SC) in brain injuries is due to the lack of effectiveness of the existing methods of therapy of such patients. The perineural method of SC implantation, in comparison with intravenous or endolumbar methods, opened the possibility of natural SC migration along cranial nerves (olfactory and trigeminal nerves) into the cranial c...

Chemotherapy is one of the main treatment options for cancer, but it is usually accompanied with negative side effects. The classical drugs combination with synergistic adjuvants can be the solution to this problem, allowing reducing therapeutic dose. Elucidating the mechanism of adjuvant action is of key importance for selection of the optimal age...

Fully optimized structures of the fullerenol (Fl=C 60 (OH) 24)-cisplatin conjugate (Fl + Cis) were obtained using the DFT simulation. The DFT/B3LYP/SV/Lanl2DZ level of theory has been used. From the results of calculation of the structural parameters, it can be concluded, that the interaction of Fl with Cis in vacuum leads to the increase of the Pt...

To record the content of nitric oxide (NO) and copper in the brain tissues (frontal lobes and hip-pocampus) and liver of healthy rats and rats after ischemia modeling, the method of electron paramagnetic resonance (EPR) spectroscopy was used. Modeling of ischemia was carried out by ligation of the carotid arteries, followed by taking 3 mL of blood...

Представлено квантово-химическое моделирование нонъюгата карбоплатин-фуллеренол как перспективной основы противоопухолевой терапии

Biological activity of Fullerenol – Cisplatin Conjugate as Agent of Antitumor Therapy: DFT simulation was described.

A comparative experimental analysis of intensity of nitric oxide (NO) production and the copper content in the tissues of hippocampus of male Wistar rats after modeling of hemorrhagic stroke and brain injury was conducted using EPR spectroscopy. Modeling of hemorrhagic stroke was carried out by microinjection of 500 nl of autologous blood into the...

The acylation of glycylglycine and its morpholide with 4,5-dichloroisothiazole-3-carbonyl chloride gave the corresponding N-(4,5-dichloroisothiazole-3-ylcarbonyl) derivatives. N-[5-(p-Tolyl)isoxazole-3-car-bonyl] analogs were synthesized by acylation of glycylglycine and its morpholide with 5-(p-tolyl)isoxazole-3-carbonyl azide since treatment of t...

Abstract⸺In order to simulate apoptotic processes in the central nervous system and retina, one eyeball was enucleated in each of the male Wistar rats (n = 10) aged 10−12 weeks. Simultaneously, in one of the series of experiments (n = 5), 100 000 PKH67-labeled mesenchymal stem cells (MSCs) in 100 µL of buffer solution were injected parabulbarically...

We present an experimental realization and characterization of artificial spiking neuron based on an optoelectronic pair “microcavity laser-single photon avalanche diode” operating in few photon regime. We show that basic properties of biological neurons, such as an existence of the threshold and the refractory period, the insensitivity to the effe...

Introduction. With a decrease in the oxygen content in the inhaled air, violations of the cerebral blood flow, brain ischemia occurs, which can end in an ischemic stroke. Aim. Comparative analysis of the intensity of nitric oxide (NO) production and the copper content in the olfactory bulb tissues of the brain of male Wistar rats after modeling an...

A real situation on the soccer field: Danish soccer player Christian Eriksen collapses on the field. Doctors do mouth-to-mouth resuscitation. Doctors have not thought about the problem of SARS-CoV-2 infection at this point. This is typical of doctors' tactics. That's what we were taught in medical school. Delay in these situations is fatal for the...

Cell technologies are one of the modern methods of therapy in retina diseases. Stem cells (SC) are injected into systemic circulation or intravitreally, which is sometimes accompanied by side e ects or ine ective. Many pathological processes in retina (retinopathy, macular degeneration, retinal angiopathy and others) require the use of really e ect...

Intriguing role of "little brain" neurons of heart in control of myocardium functional state is analyzed with addiction after appearance of this term in the articles by J.A. Armor on the border of the second and third millennia. The "little brain" neural network of heart modi es adrenergic and cholinergic a erent and e erent signals in accordance w...

Relevance. The problem of effective prevention and treatment of traumatic brain injuries (TBI) of various etiologies has not been resolved in all countries of the world. Primary brain damage from trauma initiates secondary damage to the nervous tissue. As a result, the interaction of brain neural networks is disrupted and the control of somatic and...

It was shown by electron paramagnetic resonance (EPR) spectroscopy that one day after bilateral occlusion of the common carotid arteries (to simulate ischemia) in Wistar rats (group 1, n = 15) under ketamine-xylazine-acepromazine anesthesia (55.6, 5.5, 1.1 mg/kg, respectively, intraperitoneally), the content of nitric monoxide (NO) in the olfactory...

Results of analysis of nitric oxide and copper content in rat liver and hippocampus after brain isch-emia modeling are provided. The studies are carried out using the electron paramagnetic resonance spectros-copy method with spin traps. It was shown that, the day after brain ischemia modeling, nitric oxide content in hippocampus decreases on averag...

Using the electromyography data, the functional state of the maxillofacial region muscles of dental patients with bruxism signs and obstructive sleep apnea syndrome was defined. After dental treatment, 122 patients aged 25–47 with bruxism signs and obstructive sleep apnea syndrome and without it were examined. The disease duration was about 5 years...

The relationship of undifferentiated tumor and non-tumor cells is sometimes accompanied by paradoxical processes. In rat experiments, C6 glioma cells were implanted into the brain of 30 Wistar rats. During the operation, several rats were intranasally injected with 400 thousand mesenchymal stem cells (MSCs) labeled with CD90 with FITC. One day afte...

Structural changes in thalamus neuropil after feeding animals with extrusion foods were demonstrated in experiments on 14 Wistar rats. A comparison of these data with previously obtained results on the imbalance of intestinal microflora in similar experiments was the basis for arguing the hypothesis on the key importance of functional state of micr...

Experiments on 7 Wistar rats were performed to verify the hypothesis on significance of impaired process of feces evacuation from colorectal intestine. Local damage of somatic and visceral nerves in the anus region resulted in violation of defecation process and accumulation of feces in expanded colorectal parts of intestine in one week of observat...

A comparative experimental analysis by EPR spectroscopy of the intensity of nitric oxide (NO) production and the content of the copper in the tissues of olfactory bulb of the brain of male Wistar rats were performed after modeling of ischemic stroke and treatment with immediate intranasal injection of mesenchymal stem cells (MSCs). Brain ischemia w...

Pathological processes in cardiovascular system and liver are fatal for citizens of all over the world. New preventive and therapeutic approaches are needed in this field of medicine. Using of cell technologies for the treatment of patients with heart attack and/or cirrhosis is the one. Systemic or intraorganic stem cells (SCs) administration has s...

An energy-effective mobile infrared cabin has been developed for activating the functional reserves of a human organism in medicinal, health-improving, and sports practice has been developed. The cabin maintains deep penetration of optical radiation into the tissues of a human being by using near infrared band radiators. The distinctive feature of...

Direct measurements of the content of nitric oxide (NO) by the method of spectroscopy of electron paramagnetic resonance were performed. It was found that NO production in hip-pocampus tissues decreased to 46% after 5 h of ischemic stroke as compared to the control group and remained at the same level after 72 h. Following the hemorrhagic stroke, t...

Information is accumulating in the literature stating that mesenchymal stem cells begin to move into the zone of brain neurodestruction after intranasal implantation. This aspect is very important for evaluating the obtained experimental data on the survival rate of rats up to six months after implantation of a high-grade C6 glioma tumor into the b...

The weekly Audio Interview and Online Discussions organized by the New England Journal of Medicine (NEJM) Editors on COVID-19 (NEJM.ORG) also include the question that appears in the title of this article. Severe acute respiratory syndrome COVID-19 targets clinicians primarily to the processes in lung tissue.1-3 In other cases, however, clinical ev...

Experiments on 33 Wistar rats were performed to test two interrelated hypotheses. Firstly, is the migration of fluorescein isothiocyanate (FITC) labeled endotoxin (Escherichia coli lipopolysaccharide, LPS) (5.0 μg in 5.0 μl of sterile water) via olfactory nerve into the brain after single submucous injection of LPS real? Secondly, in case such migr...

Non-small cell lung cancer (NSCLC) cells of a specific patient N have been analyzed to determine sensitivity to cisplatin. Intraoperative material was examined under sterile conditions in a culture box after pretreatment of tissue pieces from the primary NSCLC tumor and from metastases to the lymph nodes of lung root. NSCLC tumor cells were cultiva...

Experiments were performed on 28 sexually mature Wistar rats divided into two equal groups. Rats were fixed in stereotactic apparatus and received 2.8×105 C6 glioma cells in 20 μl of F10 medium locally into frontal region of brain under ketamine-xylazine-acepromazine anesthesia. One group of rats (n=14) was implanted with 40×104 MSCs in 40 μl of lo...

There is a clear understanding of some mechanisms underlying the efficacy of the conjoint action of heterocyclic compounds and classical chemotherapeutic agents. This is further supported by the experimental results demonstrating that addition of the heterocyclic compounds allows decreasing the effective dose of chemotherapeutic agents by one or ev...

Abstract i. Sensitivity of glioma cells to chemotherapeutic agents, Nerve Growth Factor, and Dendrimers, Diamond like coated samples, heterocyclic compounds in vitro depends on the origin, histological type tumors, the degree of malignancy, age and individual characteristics of patients; ii. Combined application of Nerve Growth Factor and chemothe...

Abstract A deeper understanding of mechanisms governing the functioning of Heterocephalus glaber’s systems (a naked mole-rat) will make it possible to advance in solving the fatal problem of oncological pathology. To summarize the above, it is advisable to emphasize the well-known truth about the attractiveness of scientific discussions and disput...

ABSTRACT The problem of radical therapy for cancer patients has not yet been resolved. The lack of a clear idea of the mechanisms of carcinogenesis is one of the reasons for this situation in oncology. The search for new therapies in oncology continues along with the search for an answer to the question of the origins of uncontrolled proliferation...

Paper summarizes results of cold c plasma use in medical devices surface treatment, focusing at manufacture applications. Attention is given to results of changes in the hydrophilic properties of glass surfaces, stainless steel, polyamide and latex when exposed to atmospheric plasma, depending on the time of treatment with cold plasma and distance...

Citation: Shanko Y, Smeyanovich A, Ta-nin A, Sych E, Smeyanovich V, Bulgak U, Hubkin S, Zamaro A, Navitskaya V, Kulchitsky V. Contemporary Approach-es to Diagnostics and Treatment of Fo-ramen Magnum Meningiomas. Biomed J Sci & Tech Res 18(1)-2019. BJSTR. MS.ID.003081. DOI: 10.26717/BJSTR.2019.18.003081 34 cases of cranio cervical junction area meni...

Abstract Introduction: Cell technologies represent a perspective direction due to activation of endogenous reparative processes. Correction of impaired brain functions using stem cells is one of the most effective methods in complex therapy of neurodestructive processes. Methods: Endoscopic perineural application of autologous mesenchymal stem cell...

Pericardial fragments obtained during eight open heart surgical procedures on children with atrial and/or interventricular septum defects have been investigated. Presence of clearly marked pores was established in all fragments of pericardium. The total area of original shunts was 5-6% of pericardial samples area. Signs of pericardial calcification...

Bioprinting technologies and cell technologies are being developed to correct and / or restore the activity of various functional systems of the body. The paper focuses on the effective restoration of the functional unity of the nervous system and the whole body after injury. At the same time, the newly formed construction of reparation elements is...

One of the unresolved issues in the problem of artificial intelligence is the question of the desirability of introducing elements of motivation and emotions into artificial neural networks. The attitude to this issue is still controversial. The coding system in the interfaces is based on the binary system of the "on-off" type. In the case that an...

The article briefly describes advantages of autologous mesenchymal stem cells (MSCs) use in clinical conditions to correct structure and function of damaged areas in organs and tissues of humans and animals. The effectiveness of MSCs migration via per-ineural nerve spaces to inner organs was confirmed. Such method of cell therapy allows avoiding ma...

The problem of the functional reparation of injured internal organs, especially vital ones (heart, lungs) is socially significant, since it has not been solved until now. What body reserves and practical skills of clinicians are the bases for new ideas aimed at preventing and/or restoring impaired functions of internal organs? For the authors of th...

Perineural migration of stem cells (SCs) is the basis for the development of new ap-proaches for internal organ diseases treatment. The authors have previously proved in ex-perimental and clinical conditions the effectiveness of perineural migration of SCs through the cranial nerves in strokes and brain injury conditions. In recent studies, the fea...

Kulchitsky Vladimir, Zamaro Alexandra, Koulchitsky Stanislav. Perineural Migration of Stem Cells in the Model of Damaged Heart Nodes. Biomed J Sci & Tech Res 14(4)-2019. BJSTR. MS.ID.002578. Unilateral vagotomy (n=15) with simultaneous injection of 10 thousand stem cells in 10µl of culture medium to intact vagus nerve (n=9) was performed on Wistar...

ARTICLE INFO Hypothesis on reality of perineural migration of stem cells (SCs) along vagus nerve fibers to injured heart and brain stem regions was experimentally verified on ten Wistar rats. Heart injuries were simulated using unilateral vagotomy. Vagotomy led to degeneration of vagus nerve fibers in heart and brain stem. These destructive process...

ABSTRACT. It is advisable to carefully update traditional methods of classical therapy of neoplasms with modern techniques. The existing problem of peculiar fatality for a person with a diagnosis of malignant neoplasm must be reversed combining the efforts of oncologists with professionals in the field of cell technologies, immunology, molecular bi...

Abstract Zamaro A, Krivenchuk D, Denisov A, Koulchitsky S, Kulchitsky V. Topical Issues of Bioprinting Technology. Biomed J Sci & Tech Res. 2018; 12(1) 1-3. BJSTR. MS.ID.002195. DOI: 10.26717/ BJSTR.2018.12.002195. Brief analysis of positive and negative aspects of bioprinting was performed. Bioprinting has technical deficiencies and a range of unr...

Kletskov AV, Potkin VI, Kolesnik IA, Petkevich SA, Kvachonak AV, Dosina M.O., Loiko DO, Larchenko MV, Pashkevich SG, Kulchitsky VA (2018) Synthesis and Biological Activity of Novel Comenic Acid Derivatives Containing Isoxazole and Isothiazole Moieties. Natur. Product Communication (NPC) 13(11):1507-1510. Abstract: Methyl 5-hydroxy-4-oxo-4H-pyran-2-...

Kulchitsky V., Zamaro A., Zaykina N., Yaromenka Y., Hudny G., Krivenchuk D., Semenik-Philipovich T., Koulchitsky S., Andrianova T., Dosina M., Kaliadzich Z. (2018) The Role of Feedback in Breathing Self-Regulation in Health and Obstructive Sleep Apnea Syndrome Development. Biomed J Sci & Tech Res 11(3): 1-3. BJSTR. MS.ID.002103. DOI: 10.26717/ BJST...

Abstract Zamaro A. Zhukava T., Kulchitsky V. Antitumor effects of allogenic mesenchymal stem cells. Arch Cancer Res. 2018. Vol. 6. DOI: 10.21767/2254-6081-C5-018 In conditions of ketamine-xylazine-acepromazine anaesthesia (55.6, 5.5, and 1.1 mg/kg respectively), Wistar rats (n=25) after scull trepanation of sensorimotor zone were implanted with 100...

Kulchitsky V, Zamaro A, Navitskaya V, Koulchitsky S, Shanko Y. Regulatory Effects of Mesenchymal Stem Cells in Brain. Biomed J Sci&Tech Res. 2018. 11(1): 1-3. BJSTR. MS.ID.002039. DOI: 10.26717/ BJSTR.2018.11.002039. Abstract The question on various functional effects of mesenchymal stem cells (MSCs) during different pathological processes developm...

Kulchitsky V, Zamaro A, Shanko Y, Koulchitsky S. Prospects of Perineural Implantation of Stem Cells in Recovery of Neural Networks’ Functions in Brain Diseases. Biomed J Sci&Tech Res. 2018. 10(3):1-4. BJSTR. MS.ID.001959. DOI:10.26717/ BJSTR.2018.10.001959 Abstract Background: The question on prospects of additional use of cellular therapy in stand...