Vivian Panes

The University of Maine, Orono, Maine, United States · Hitchner Hall, School of Biology and Ecology

Moringa oleifera Lam. is a resilient plant that is adaptable to a wide range of environmental conditions from dry to hot, wet and humid conditions. However, there is a dearth of information on the stress response genes of M. oleifera in published literatures. The main objectives of this study were to identify the stress response genes in the transc...

This is the first report on the novel DNA barcode sequences, matK and ITS genes, to authenticate the seven outstanding Canarium ovatum varieties-namely, Lanuza, Laysa, Magayon, Magnaye, Mayon I, M. Orolfo, and Orbase. These varieties are registered under the National Seed Industry Council (NSIC) and cultivated at the Pili Research and Technology De...

Moringa oleifera Lam. (Malunggay), is a vegetable and medicinal plant used in many Southeast Asian countries. Parts like the leaves and seeds are known to contain antioxidants. However, genes and transcripts involved in antioxidant biosynthesis are yet to be studied. This study aims to identify and analyze transcripts from M. oleifera mature seed e...

This study looks at the molecular basis of plant response to climate change-related stress through transcriptome analysis of the mature embryos of Moringa oleifera Lam., a species with high nutritional value and various medicinal properties. This was done by identifying and analyzing transcripts assembled from 88951 and 182591 sequences using Oases...

Moringa oleifera is a widely used crop that produces seeds with a plethora of benefits encompassing health and nutrition. Secondary metabolite compounds were determined in the seeds of Moringa oleifera that possess nutritional and pharmacological benefits. Although various phytochemical researchers reported the presence of secondary metabolites in...

Isolation of chloroplast is essential for studying the organelle’s physiological activities in photosynthesis, electron flow, phosphorylation and carbon assimilation. Chloroplast DNA is useful in solving problems in phylogeny, identification of organisms and creating accessions of molecular data for organisms that are vulnerable to extinction and d...

Saccharomyces cerevisiae is a model organism used for studies, such as molecular biology and biotechnology. Due to its simplicity, it has provided many insights on gene expression, DNA repair, and proteolysis of eukaryotic organisms. The aim of this study is to successfully extract and amplify DNA from yeast, using the Xanthogenate method and polym...

Chitin deacetylases have been identified and studied in several fungi and insects but not in crustaceans. These glycoproteins function in catalyzing the conversion of chitin to chitosan by the hydrolysis of N-acetamido bonds of chitin. Here, for the first time, the full length cDNA of chitin deacetylase (CDA) gene from crustaceans was fully cloned...

Rice germplasm conservation is vital to ensuring the availability of a rich gene pool for future varietal improvement programs. However, with limited resources for gene banking, there is a need to identify and prioritize unique accessions in the PhilRice gene bank for maximum resource utilization. A robust and unequivocal way to identify duplicates...

Lectins constitute a class of proteins that bind reversibly to mono and oligosaccharides. Studies conducted on lectins support the claims of its antiviral, anti-bacterial, antifungal and anti-insect activities. Although lectins cannot alter the structure and permeability of the cell membrane and cannot change the normal intercellular activity of ba...

DNA stands for Deoxyribonucleic Acid. It is found in the nucleus of the cell of eukaryotic cells and in the cytoplasm particularly in the region called the nucleoid (devoid of a membrane) in prokaryotes. DNA contains hereditary units called genes. The genes in turn are considered the blueprint of life. It is the main reason why each individual is u...

Identification and Cloning of a Curcin-Like Ribosome Inactivating Protein (RIP) Gene in Jatropha podagrica Hook. and Jatropha pandurifolia Andr. V.A. Panes and M.Q. Corpuz Department of Biology, School of Science and Engineering Ateneo De Manila University, Loyola Heights, Quezon City 1108, Philippines Ribosome-inactivating proteins (RIPs) inhibit...

The relationships among Allium L. species remain complex. Certain species belonging to the genus Allium are medically and economically important and as such merit phylogenetic analysis. This study aimed to determine the interspecific genetic variation and phylogeny of the six species of the genus Allium L. namely: A. ascalonicum L., A. cepa L., A....

Geometric morphometry was used to assess the variations in the shapes of the leaves in Ceriops tagal (Perr.) C.B. Rob. The technique enabled to understand the shapes of the leaves through Elliptic Fourier Descriptors (EFD). The outline-based morphologies of the leaves from the third order branch located at the crown. Lateral inflorescence and base...

Resistance genes represent key entry points for probing into the mechanism of protection against the rice tungro disease. Some of these resistance genes have been identified in some resistant cultivars out of thousands of accessions screened. Two of the most highly resistant cultivars are the Indian cultivar ARC 11554 and the Indonesian cultivar Ut...

Resistance genes represent key entry points for probing into the mechanism of protection against the rice tungro disease. Some of these resistance genes have been indentified in some resistance cultivars out of thousands of accessions screened (Hibino et al., 1990). Two of the most highly resistant cultivars are the indian cultivar ARC11554 and the...

In the Philippines, considerable variation in rice wine quality is often obtained between and among rice wine producers. Microbial composition of rice wine starter is critical to maintaining the quality of wine. To better understand the microbial ecology, yeast strains were isolated from starters obtained from three areas in Ifugao province for mor...

The effects of light intensity of the frequency and distribution of calcium oxalate crystals in leaves of Azadirachta indica, or "neem" trees, are investigated. Using a photometer, light intensity was measured at the apex of the canopy, which was directly exposed to sunlight (sun leaves), at the center of the canopy (shade leaves), and at the base...

Allium sativum L. commonly known as garlic, is an important and widely cultivated plant with both culinary and medicinal uses. In this study, morphological data such as bulb siz, bulb weight, number of scales, number of cloves, weight of cloves and growth rate of garlic samples from ten different sites in the Philippines were collected. Genomic DNA...

Leaves of four plant species, viz., Bougainvillea spectabilis Willd., Hibiscus rosa-sinensis Linn., Mangifera indica Linn. and Psidium guajava Linn. from the vicinity of three cement factories (Central Cement and Hi-Cement in Bulacan; Rizal Cement in Rizal) and from apparently relatively unpolluted areas (Montalban, Rizal and Pulilan, Bulacan) were...

Moringa oleifera Lam. seeds are capable of producing 40% edible oils that are gaining importance due to its superior stability and nutritional benefits. Many studies have examined Moringa oleifera seed oil, however, these studies focused on the extraction of oil and methods of biodiesel production. There is a dearth of information on transcriptome...

MADS-box genes are dominant organ identity genes represented through the whole genome of plants which encode for MADS domain transcription factors. Moringa oleifera Lam. is a tree with conspicuous white to cream flowers. Flowering begins in January and continues to March. The pods contain black winged seeds. Genes which control flowering and seed d...

Seeds of Moringa oleifera Lam. have high oleic acid which is a good source of polyunsaturated oils. Seed oils are made up of triacylglycerols (TAGs) stored in the oil bodies with oil body associated proteins that support seed germination processes. Proteins associated with M. oleifera oil bodies have not been reported previously. This study aimed t...