Vivian Lobo

Vivian Lobo
Indian Council of Medical Research | ICMR

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15
Publications
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178
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Publications

Publications (15)
Article
Glucose regulated protein 78 (GRP78) is expressed on cell surface in exceptional conditions as seen in cancer cells and macrophages. We have reported its membrane localization in sperm. The functional significance of its surface localization in sperm is an enigma. Alpha-2-macroglobulin (α2M) reportedly binds surface GRP78, regulating macrophage mot...
Article
Full-text available
GRP78, a resident endoplasmic reticulum (ER) chaperone involved in protein transport, folding and assembly, has been reported in sperm. It is shown to be localized in the neck region of human sperm. We have previously reported GRP78 to be less phosphorylated in asthenozoosperm.The present study aimed to determine whether sperm GRP78 undergoes phosp...
Data
Optimization assays for GRP78 phosphodetection in sperm. (DOCX)
Data
Effect of Urea on the GRP78 profile in rat testicular- and caudal sperm. A representative isoelectropherogram of overlaid profiles of GRP78 for testicular sperm (A) and Caudal sperm (B) using 20ng of protein lysate made in lysis buffer containing No Urea (blue), 6M (red), and 9M (green) of Urea. Three peaks were observed for testicular- and four pe...
Data
Effect of Phosphatase Inhibitors present in NP40 lysis buffer on the outcome of λ-PP treatment of rat sperm lysates. This was studied by using testicular- or caudal sperm lysates prepared in NP40 lysis buffer containing phosphatase inhibitor cocktail or devoid of it, and incubating 100 μg of these lysates without or with 300U of λ-PP at 30°C for 2...
Data
NIA profile of GRP78 in rat sperm using Premix of pI range 5–6. A) A representative isoelectropherogram of GRP78 profile in testicular sperm (red). Three peaks GP4.94, GP4.96 and GP5.43 were consistently observed for testicular sperm. In caudal epididymal sperm (blue), four peaks GP4.85, GP4.94, GP4.96 and GP5.43 were consistently observed. ‘GP’ re...
Data
Effect of different concentrations of λ-PP on GRP78 peak profile in rat testicular- and cauda epididymal sperm. Representative isoelectropherogram depicting overlaid images of NIA profiles for testicular- (A) and caudal sperm (B) treated without or with 100–500 U λ-PP. 100 μg of testicular- or caudal sperm protein was incubated without (red) or wit...
Article
A sensitive and specific sandwich ELISA was developed for the diagnosis of Glanzmann's thrombasthenia (GT) and the heterozygote carriers of the disease using whole blood platelets. The assay used anti-CD36 antibody to capture platelets from platelet-rich plasma which was subsequently treated with a bioengineered disintegrin/alkaline phosphatase hyb...
Article
Sperm motility is an important prerequisite for successful fertilization and is regulated by cyclic AMP activated protein kinase A which phosphorylates flagella proteins like axonemal dynein and initiates motility. Increase in calcium influx reverses this process by dephosphorylation that is mediated by calcineurin. Analyzing the phosphoenriched fr...
Article
A sensitive and specific sandwich ELISA was developed for the diagnosis of Glanzmann's thrombasthenia (GT) and the heterozygote carriers of the disease using whole blood platelets. The assay used anti-CD36 antibody to capture platelets from platelet-rich plasma which was subsequently treated with a bioengineered disintegrin/alkaline phosphatase hyb...

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