Vitaly Goranov

Bio Device Systems

The in silico platform for the initial prediction of safety and efficacy of (bio-)material based on in vitro tests is elaborated. The set of bio-kinetic (in vitro) models serves as a tool, which is developed to facilitate follow-up steps for in vitro-in vivo extrapolation and prediction of biomaterial risk for the human organism. The service is des...

Multifunctional and resistant 3D structures represent a great promise and a great challenge in bone tissue engineering. This study addresses this problem by employing polycaprolactone (PCL)-based scaffolds added with hydroxyapatite (HAp) and superparamagnetic iron oxide nanoparticles (SPION), able to drive on demand the necessary cells and other bi...

The gelatin nanoparticles saturated with magnetoferritin were not toxic for hMSC at concentrations equal to or less than 500 pg/cell in vitro. Moreover, these nanoparticles were internalized by cells and made them magnetically susceptible.

The proposed mathematical model set gives a possibility to evaluate the kinetics of cell death and allows estimating the cytotoxicity of the biodegradation products of a degradable material by analysis of in vitro data. These models are applicable in the cases when the rate of cell death is proportional to the concentration of the toxic products of...

Our research focused on the role of immune mechanisms in diabetic foot ulcers development and impaired healing. A 3D skin culture system, as a relevant skin model, has proved valuable in investigating these mechanisms and is a useful tool to study interactions between keratinocytes, fibroblasts and immune cells. The aim of this study was to evaluat...

At the moment, a lot of scientific research focused on the role of immune mechanisms in diabetic foot ulcers development and impaired healing. A 3D skin culture system as a relevant skin model may prove valuable in investigating these mechanisms and may be a useful tool to study interactions between different cell types such as keratinocytes, fibro...

A three dimensional magnetic patterning of two cell types was realised in vitro inside an additive manufactured magnetic scaffold, as a conceptual precursor for the vascularised tissue. The realisation of separate arrangements of vascular and osteoprogenitor cells, labelled with biocompatible magnetic nanoparticles, was established on the opposite...

Detection of biomarkers in picomolar or subpicomolar concentrations for revealing marker proteins at the early stage of neurodegenerative diseases is an urgent problem. One of the stages of its solution is determination of the marker’s protein layer thickness adhering to the surface of magnetized nanoparticles. In this work, we present an algorithm...

Thermo switchable magnetic hydrogels undoubtedly have a great potential for medical applications since they can behave as smart carriers able to transport bioactive molecules to a chosen part of the body and release them on demand via magneto-thermal activation. We report on the ability to modify the lower critical solution temperature (LCST) of po...

The mathematical prediction of cell proliferation in porous scaffold still remains a challenge. The analysis of existing models and experimental data confirms a need for a new solution, which takes into account cells" development on the scaffold pore walls as well as some additional parameters such as the pore size, cell density in cellular layers,...

We studied structural and magnetic proprieties of the fulleride C60(FeCp2)2. The influence of fulleride particles on the cell proliferative activity was also investigated. We found that the proliferative activity of the RINmF5 cells increases (53% versus control) in presence of the C60(FeCp2)2 nanosized particles. Moreover, it was registered that t...

We studied structural and magnetic proprieties of the fulleride C60(FeCp2)2. The influence of fulleride particles on the cell proliferative activitywas also investigated.We found that the proliferative activity of the RINmF5 cells increases (53% versus control) in presence of theC60(FeCp2)2 nanosized particles.Moreover, it was registered that the c...

Zirconia-alumina ceramic foam scaffolds with a nanocrystalline HAP coating were used for the preparation of integrated motile orbital implants. This study demonstrated that open-cell ceramic foams with enhanced strength-to-density ratio are quite suitable as biocompatible materials for the manufacture of orbital implants for post-enucleation syndro...

The aim of this study was elaboration and morpho-functional investigation of the thyrocyte culture from rabbit fetal thyroid gland. The optimal method of the thyrocyte culture preparation was designed which are developing through a monolayer cytotypic into organotypic culture (“mini thyroid gland”). Thyroid cellular cultures save its morphological...

The optimized method of cell dissociation from human parathyroid glands has been proposed. To preserve the tissue and to enhance cell viability the cell isolation with pre-incubation at 4C with the enzymes was realized. The results obtained in this study can be used to investigate the structural and functional properties of parathyroid cells in lon...

In this work we studied structural, magnetic, optical and some other proprieties of fullerides C 60(FeCp 2) 2, C 60 (NiCp 2) 2 and their derivatives. The influence of fulleride particles on the cell proliferative activity was also investigated. We found that the proliferative activity of the RINmF5 cells increases (53% vs. control) in the presence...

The necessity of new clinical approaches regarding musculoskeletal system regeneration is evident. Nowadays different strategies such as autografts, allografts also used in synergy with cell therapy are already used in clinical treatments for moderate defects, but they face significant limitations due to limited supply, and risk of immune rejection...

In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the use of these conventional scaffolds lies in the impossibility of re-loading...

The motility of cell suspension components after fermentative treatment of pancreatic tissue has been investigated. The new approaches of different cells and debry components separation under magnetic field influence were proposed.

To evaluate intravascular xenotransplantation of macroencapsulated pancreatic islet cells in the process of the treatment of diabetes mellitus type 1 without immunosuppressive therapy. Xenogenic intravascular transplantation was performed in 19 patients with diabetes mellitus (type 1) having high insulin demand and frequent hypo- and hyperglycemic...

In cross-sectional case-control study we have evaluate the cytotoxicity index (CTI) as a marker of endotoxemia status in eating disorders' patients (anorexia nervosa, n=83; bulimia nervosa and binge eating disorder, n=42; healthy control, n=36). It has been point out that eating disorders patients are strongly different from age- and sex-matched co...

Some new approaches in treatment of insulin-dependent diabetes mellitus suggest a combination of cell therapy methods with genetic modification of transplanted cells. The use of cells with integrated insulin genes or b-cells with genes of resistance to different damaging agents, such as soluble blood factors (cytokines, complement molecules, antibo...

The review is concerned with the investigations of islet cell resistance in different conditions. A new conception of islet cell viability in integrated system has been discussed. Basic researches on adaptative cell transplantation were described.

Experimental dogs with alloxan-induced diabetes mellitus (n = 22) were transplanted into the abdominal aorta lumen with macroencapsulated xenogenic rabbit islet cells without immunosuppressive therapy. The investigation showed normalization of the blood glucose level and disappearance of diabetes mellitus symptoms. Morphological and immunohistochem...

The article presents a new method of surgical treatment of experimental diabetes mellitus in a rabbit to dog model. Rabbit islet cells, which had been macroencapsulated into a microporous polyamide, were implanted into the dog aorta without immunosuppressive therapy. Euglycemia was reached at 4 to 5 days and persisted for 12 months. Morphological a...

Morphofunctional parameters and markers of apoptosis were studied in a model of infection caused by scrapie agent (strain 263K) in the rat glioma cell culture (C6) and in hamsters. It was established that astrocytes were target cells for the scrapie agent. Reactions of astrocytes during infectious process were similar in animal CNS and glial cell c...

In recent decades, the attention of biologists and doctors of various specialties has been attracted by the phenomenon of apoptosis a genetically programmed process of death and utilization of committed cells with the participation of biochemical reactions controlled by the whole organism [6 |. This process starts as a result of the interaction of...

It has been suggested that there exists a local immune-neuroendocrine self-regulating system in the pancreas. The system consists of beta-cells, nerve ganglia, intercellular fluid, connective tissue, and endothelial and immunocompetent cells. The local immune-neuroendocrine system governs the background level of insulin production by intrinsic mech...

The influence of streptozotocin and interleukin-1 on survival of beta-cells in vitro was investigated. Interleukin-1 essentially potentiate the damage effect of subtoxic concentration of streptozotocin.

It is suggested that the development of insulin-dependent diabetes mellitus (IDDM) is due to insufficiency of local immunoneuroendocrine system (LINES), which prevents development of the pathological process in the insular tissue at the very early stages. The further disbalance between LINES and CNS and development of pathology occurs with a very a...

High potential of Melatonin for different clinical applications is discussed.

It is suggested that the development of insulin-dependent diabetes mellitus (IDDM) is due to insufficiency of local immunoneuroendocrine system (LINES), which prevents development of the pathological process in the insular tissue at the very early stages. The further disbalance between LINES and CNS and development of pathology occurs with a very a...