Vishnu Prabhakar ChoudhariSchool of Health Sciences and Technology , MIT World Peace University · Pharm. Analysis and Quality Assurance
Vishnu Prabhakar Choudhari
M. Pharm., Ph. D.
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81
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Introduction
Vishnu Prabhakar Choudhari currently works at the Quality Assurance Dept., School of Pharmacy, MIT WPU, Kothrud, Pune. Vishnu does research in Science Education, Phytochemistry, anticancer and antidiabetic new drugs, Metal oxides, and Nanotechnology. Their current project is 'Mixed metal oxides in therapeutics, herbal standardization, anti diabetic drug-drug interactions, antidiabetic conjugate and analytical method development.
Additional affiliations
September 2001 - present
Publications
Publications (81)
The study was undertaken to evaluate single herb formulation (SHF) as Saptarangi tablet (ST) and polyherbal formulations (PHF) as Saptarangi plus Kadha (SK) for diabetes and hyperlipidemia. The main objective of proposed study was to evaluate the anti-diabetic and anti-hyperlipidemic activities of ST and SK in streptozotocin (STZ)-induced diabetic...
A new high-performance thin-layer chromatography (HPTLC) method for the determination of adapalene and phenoxyethanol was developed and validated. An excellent resolution with RF values 0.43 ± 0.03 and 0.75 ± 0.02 for adapalene and phenoxyethanol was achieved by using optimized chromatographic conditions. A mixture of toluene‒acetone (8:2, V/V) was...
Diabetes mellitus is a metabolic disorder in which a person fails to produce adequate insulin or insulin reactions to cells, so that blood sugar levels are high in diabetes patients. Nanoparticles of small particle size have very significant effects against other types of dosage. Diabetes is treated with metal oxide-like nanoparticles such as zinc...
This paper describes the process of extracting ethanol from Jatropha curcas and its various blending effects on spark-ignited engine performance for environmental sustainability. Alternatives to conventional fuel sources have to be found because of the depletion of fossil fuels and stringent regulations. Every day, the growing population and improv...
A simple stability indicating RP-HPLC method for the analysis of Adapalene and Dapsone in pharmaceutical gel formulation was developed and validated. A gradient elution was performed on an analytical column Phenomenex Kinetex C8 (150 × 4.6 mm, 5 micron) kept at 30 °C. Chosen mobile phase for the analysis was acetonitrile: water at the pH (2.5) adju...
Alternatives to conventional fuel sources have to be found because of the depletion of fossil fuels and stringent regulatory regulations. Every day, the growing population and improved transportation increase the energy demand. Agriculture and food waste management, as well as pollution, remain major issues that require attention. Bioethanol is an...
It is common for dyes to be utilized in a wide range of industries such as leather and textiles as well as the printing, paper, and packaging industry. Most dyes fall into a dangerous category of water toxins that have had a significant impact on the ecosystem. Dye removal from wastewater can be done in a variety of methods. It is now necessary to...
A simple, precise bioanalytical UPLC Tandem Mass Spectroscopy gradient elution method was developed and validated for simultaneous estimation of Olmesartan Medoximil (OLM) and Metoprolol Succinate (MET) in human plasma. The quantitation carried out using Shimadzu Shimpack-C18 GIST AQ (50 mm X 2.1 mm, 1.9 µm) column and the mobile phase A comprises...
Nanotechnology opens up new possibilities for food innovation at an incredible rate, yet new technology needs evaluations of both potential negative impacts and numerous good effects. We aimed to cover some of the latest breakthroughs in nanotechnology and their application to food processing in this overview. Applications of nanoparticles in the f...
Background
Analysis of tertiary mixtures of analytes along with their impurities with simple and cost effective manner is always of interest. Utility of chemometric techniques are growing in pharmaceuticals, it improve speediness in the analysis and also provide analytical solutions with reduce the number of steps in the analytical method. In this...
A new HPTLC method for simultaneous determination of Canagliflozin and Metformin was developed, validated and compared with the reported one. An excellent resolution with Rf values 0.21±0.02 and 0.50±0.03 for Metformin and Canagliflozin respectively was achieved by using optimized chromatographic conditions. Toluene: Methanol: Triethyl amine: Glaci...
Objectives:
Olmesartan medoxomil (OLM) and metoprolol succinate (MPS) in fixed-dose combination (FDC) tablet formulation prescribed extensively. Stability indicating (SI) method for impurities and related substance (RS) test quantitates the amount of these analytes in formulation; the manuscript presents SI/RS-ultra-high performance liquid chromat...
Non organic food and chemical intake has been associated with a variety of infections and disorders, notably cancer, digestive dysfunction, headaches, ADHD (Attention Deficit Hyperactivity Disorder), prenatal deformities, a compromised immune system and early mortality. Organic foods are pesticide-free and it is beneficial for overall health. Organ...
Euphorbia fusiformis Buch.-Ham.Ex D.Don, (Synonym: Euphorbia acaulis Roxb) is an infrequent medicinal, geophytic herb belonging to family Euphorbiaceae. The latex obtained from tubers of Euphorbia fusiformis was traditionally used as an antidote in scorpion and snake bites. Fresh latex has been externally applied to heal chronic wounds and cracks a...
Wearable devices (WDs) and mHealth (Mobile Health) give you the healthcare services, overcoming geographical, temporal or even organizational obstacles. Wearable technologies will have non-encroaching and sovereign devices that collect save and examine physiological information that will certainly help to improve patient health. It has been used so...
A successful attempt has been made to develop and validate a stability-indicating high-performance thin-layer chromatography (HPTLC) method for the simultaneous estimation of empagliflozin and metformin in pharmaceutical formulation. Excellent separation between drugs and degradation products was achieved by using toluene‒methanol‒ammonia‒glacial a...
The current study presents the synthesis and evaluation of zinc oxide and silver oxide hybrid nanoparticles for their potential apoptotic and anti-cancer activity. Owing to their small particle size, high surface area, and synergistic effects of embedded biomolecules, microwave-assisted synthesised nanoparticle-based drug delivery systems have sign...
Aim: The aim of the present study include development of validated RP-HPLC method for estimation of Empagliflozin and Metformin in combined dosage form by using LC-MS compatible volatile mobile phase. Methodology: Appropriate separation of drugs was achieved using C18 column as a stationary phase and Acetonitrile: Water (50: 50, v/v) at a flow rate...
Recently, water resources are in serious danger of being overused. In particular, water
contamination has significant environmental consequences. The processing of huge quantities of wastewater has put a lot of pressure on humanity, in addition to the scarcity of water supplies due to drought and misuse. Different kinds of water contaminants exist....
ABSTRACT:
Exogenous or external antioxidants are required in the form of a food supplements and/or medicines to protect the body from oxidative stress and ultimately from heart disease, stroke, aging and other risks specially cancer. A liquid polyherbal preparation was developed as Saptarangi Plus Kadha which contains three medicinally important he...
Acetylation and deacetylation of histone proteins is regulated through two enzymes; acetylation is mediated through histone acetyl transferase (HAT) whereas deacetylation is mediated through histone deacetylase (HDAC). Histone deacetylase removes acetyl group of lysine residue on histone proteinthis makes negatively charged histone able to bind wit...
Background
A successful attempt has been done to develop and validate a simple stability indicating HPTLC method for the estimation of Mometasone furoate (MF) and its degradation product in the presence of Salicylic acid (SA). The degradation product was isolated, characterized and tested for cytotoxicity.
Introduction
Mometasone furoate (MF) is c...
Background:
Ojamin (OJ), a polyherbal antidiabetic formulation, is extensively used as a food supplement to control diabetes alone or along with synthetic antidiabetic agents. However, it's phytochemical and pharmacological investigations are lacking.
Objective:
The present study was undertaken to study antidiabetic and antihyperlipidemic potent...
Objective: The present work describes stability indicating (SI) RP-HPLC-PDA method for determination of abiraterone acetate (ABA) and characterization of its base catalyzed degradation product by LC-MS. Methods: The separation was achieved by using column Kromasil C18 (250 mm x 4.6 mm, 4.0 µ) using acetonitrile (ACN): ammonium acetate buffer 10 mM,...
In the current trend various regulatory authorities like ICH, USFDA, Canadian Drug and Health Agency are emphasizing on the identification and quantitation of impurities in Active Pharmaceutical Ingredient’s (API’s) and it is now emerging as a mandatory requirement for the new drug application. Hence their level should be controlled and monitored....
Objective: To develop simple, rapid and precise liquid chromatographic method for simultaneous determination of Mometasone furoate (MF), Salicylic acid (SA), Methylparaben (MP) and Propylparaben (PP) form pharmaceutical preparation. Methods: Isocratic separation of MF, SA, MP and PP was achieved using Kromasil C 18 (250 × 4.6 mm, 5 µm) analytical c...
ABSTRACT
Isolation identification and characterization of the structures of degradation products is typically collaborative research involving knowledge of analytical and organic chemistry with spectroscopic information. Stability testing guidelines issued by International Conference on Harmonization (ICH) require the reporting, identification and...
Impurity profiling has gained importance in modern pharmaceutical analysis due to the fact that an unidentified, potentially toxic impurity are hazardous to health and in order to increase the safety of drug therapy, impurities should be identified and determined by selective methods. Thus a simple sensitive high performance thin layer chromatograp...
The aim of present study was to formulate and evaluate pH and time dependent colon targeted polymeric system developed using in-situ formation of interpolymeric complex between
Poly(maleic diacid-alkyl vinyl ether) Gantrez® S-97 and PVP K30. Theophylline tablets were characterized by FT-IR, DSC and were evaluated for physical parameters, assay and...
A simple stability-indicating isocratic RP-HPLC method was developed and validated for the determination of mycophenolate sodium and its alkali degradation product. Forced degradation of the drug was carried out under thermolytic, photolytic, acid/base hydrolytic, and oxidative stress conditions. Alkali degradation product DP1 was isolated, and sep...
A simple, economical, precise, and accurate new UV-visible spectrophotometric baseline manipulation method for simultaneous determination of tenofovir disoproxil fumarate (TE) and emtricitabine (EM) in combined tablet dosage form has been developed. The method is based on baseline manipulation (difference) spectroscopy where amplitudes at 261 and 2...
AIM AND BACKRGOUND: A simple, rapid, precise and isocratic RP-HPLC (Reverse Phase High Performance Liquid Chromatography) method is aimed to develop for the simultaneous estimation of Olmesartan Medoxomil and Metoprolol Succinate in bulk drug and pharmaceutical dosage form.
The quantification is carried out using YMC-Pack CN (250 × 4.6 mm, 5.0 μm)...
A simple, rapid, and precise reversed-phase liquid chromatographic method is developed for simultaneous determination of Aliskiren and Hydrochlorothiazide in their binary mixture of commercial pharmaceutical preparations. This method, reported first time for a binary mixture, uses a Kromasil C18, 250 × 4.6 mm, 5 μm analytical column. Analytes were...
A simple, precise, rapid, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of thiocolchicoside (THIO) and aceclofenac (ACE) in bulk and pharmaceutical dosage form on silica gel pre-coated aluminum 69F(254) plates (10 cm x 10 cm) with 250 mm thickness. The separation was carri...
Three simple, economical, precise and accurate UV Spectrophotometric methods have been developed for the estimation of Mycophenolate sodium in bulk and pharmaceutical formulations. Method A involves measurement of absorbance of solution at 250.23 nm in methanol. Method B involves measurement of integrated area under curve in the wavelength range of...
Simple, precise, rapid and accurate methods for simultaneous determination of Atazanavir (ATV) and Ritonavir (RTV) in combined tablet dosage form have been developed. Method A is based on Ratio spectra derivative and method B uses integrated area under curve (AUC), methanol is used as solvent for both the methods. The amplitudes at 280.01 nm and 28...
A simple, economical, precise and accurate methods for simultaneous determination of Dutasteride (DUTA) and Tamsulosin (TAM) in combined tablet and capsule dosage form has been developed. The first method is based on Ratio Spectra Derivative (Method A) and second method is based on Dual Wavelength Spectrophotometry (Method B). Methanol was used as...
Simple, precise and economical spectrophotometric method have been developed for the simultaneous estimation of Metoprolol Succinate (METO) and Amlodipine(AMLO) in combined tablet dosage form. Present study describes the development and validation for the simultaneous estimation of metoprolol(METO) and Amlodipine(AMLO) by the Ratio derivative UV sp...
Two simple, economical, precise and accurate methods are described for the simultaneous determination of Dexketoprofen (DKP) and Thiocolchicoside (TCS) in combined tablet dosage form. The first method (Method A) is Absorption Corrected Method and second method (Method B) is First Order Derivative Spectrophotometry. The amplitudes at 300.0 nm and 37...
Simple, sensitive, selective, precise stability indicating chromatographic methods for determination of Drotaverine Hydrochloride and Diclofenac Potassium from pharmaceutical tablets were developed and validated as per the ICH guidelines. A novel stabilityindicating LC assay method was developed and validated for quantitative determination of Drota...
Two simple, economical, precise and accurate methods for simultaneous determination of Drotaverine (DRT) and Nimesulide (NIM) in combined dosage form have been developed. The first method is Ratio Derivative spectroscopy method (Method A) in which ratio derivative amplitudes were measured at selected wavelengths. Second method is Dual wavelength Sp...
Three simple, economical, precise and accurate methods are described for the simultaneous determination of Mefenamic acid (MFA) and Paracetamol (PCT) in combined tablet dosage form. The first method (Method A) is Dual wavelength, second method (Method B) is Area under curve and third method (Method C) is Absorption Corrected In the First method, MF...
Simple, precise, rapid and accurate methods for simultaneous determination of Lamivudine, Zidovudine and Nevirapine in combined tablet dosage form have been developed. Method is based on Ratio spectra derivative and methanol is used as solvent. The amplitudes at 229.77 nm, 316.40 nm and 341.76 nm of the first derivative of ratio spectra were select...
Three simple, economical, precise and Accurate UV Spectrophotometric methods have been developed for the estimation of Montelukast sodium in bulk and pharmaceutical formulations. Method A involves measurement of absorbance of solution at 344.30 nm in methanol. Method B involves integrating area under curve in the wavelength range of 342.93 nm – 343...
A simple, economical, precise, and accurate new UV spectrophotometric baseline manipulation methodology for simultaneous determination of drotaverine (DRT) and etoricoxib (ETR) in a combined tablet dosage form has been developed.
The method is based on baseline manipulation (difference) spectroscopy where the amplitudes at 274 and 351 nm were selec...
To develop a simple, precise, rapid and accurate HPTLC method for the simultaneous estimation of Lornoxicam (LOR) and Thiocolchicoside (THIO) in bulk and pharmaceutical dosage forms.
The separation of the active compounds from pharmaceutical dosage form was carried out using methanol:chloroform:water (9.6:0.2:0.2 v/v/v) as the mobile phase and no i...
The aim of present study was to formulate and evaluate pH and time dependent colon targeted polymeric system developed using in-situ formation of interpolymeric complex between
Poly(maleic diacid-alkyl vinyl ether) Gantrez® S-97 and PVP K30. Theophylline tablets were characterized by FT-IR, DSC and were evaluated for physical parameters, assay and...
Three simple, economical, precise, and accurate methods are described for the simultaneous determination of Tenofovir disoproxil fumarate (TE) and Emtricitabine (EM) in combined tablet dosage form. The first method is ratio derivative spectra, second is first-order derivative spectrophotometry and third is absorption corrected method. The amplitude...
A simple, economical, precise and accurate method for simultaneous determination of Diclofenac potassium (DICLO K) and Thiocholchiside (THIO) in combined tablet dosage form has been developed. The first method is based on Absorption corrected and second method is Area Under Curve (AUC) Spectrophotometry. The first method was based on the absorption...
This article describes a new, simple, precise and accurate TLC method for si-multaneous quantitation of atorvastatin (ATO), ezetimibe (EZE), and fenofibrate (FEN) as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F 254 as the stationary phase and the so...
Two simple, rapid, accurate and economical spectrophotometric methods are described for the determination of Drotaverine (DRT) and Etoricoxib (ETR) in combined dosage form. The first method (Method A) is based on Ratio Spectra Derivative was measurement of amplitude of first order derivative of ratio spectra where DRT and ETR were determined at λ m...
A simple, selective, rapid, and precise RP-HPLC-PDA method has been developed for the simultaneous estimation of Lornoxicam (LOR) and Thiocolchicoside (THIO) in pharmaceutical dosage form by reverse phase liquid chroma-tography using Waters Symmetry C18 (250 mm × 4.6 mm, 5.0 μ) column. The mobile phase consisting of metha-nol: THF: acetate buffer (...
A simple, economical, precise and accurate method for simultaneous determination of Clopidogrel (CLOP) and Aspirin (ASP) in combined tablet dosage form has been developed. The first method is based on Ratio Spectra Derivative (Method A) and second method is based on Area under Curve (AUC) Spectrophotometry (Method B). The amplitudes 218.11 nm and 2...
A simple, precise and accurate methods for simultaneous determination of Cefixime (CEF) and Ofloxacin (OFL) in combined tablet dosage form has been developed. The method is based on Ratio spectra derivative and Area under curve (AUC) spectrophotometry using methanol and 0.1 N HCl, respectively as solvents. The amplitudes at 319.11 nm and 347.40 nm...
Two simple and sensitive spectrophotometric methods are described for the determination of Montelukast sodium and Levocetirizine Dihydrochloride in combined dosage form. First method was first order derivative spectroscopy where montelukast and levocetirizine were determined at λmax 340nm and 331nm, respectively in methanol. The second method was b...
A simple, rapid and precise reversed-phase liquid chromatographic method is developed for simultaneous
determination of Atorvastatin, Ezetimibe and Fenofibrate in their ternary mixture of commercial pharmaceutical preparations. This method, reported first time for a ternary mixture, uses a Kromasil C18, 250 × 4.6 mm, 5μm analytical column. Analytes...
A simple, economical, precise and accurate method for simultaneous determination of Drotaverine (DRT) and Aceclofenac (ACE) in combined dosage form has been developed. The first method is Ratio Derivative spectroscopy method (Method A) in which ratio derivative amplitudes were measured at selected wavelengths. Second method is Area under Curve Spec...
A simple, rapid, economical, precise and accurate method for simultaneous determination of Aspirin (ASP) and Ticlopidine (TIC) in combined dosage form has been developed. The first method was First Order Derivative Spectroscopy (Method A) in which derivative amplitudes were measured at selected wavelengths. Second method was Area Under Curve (Metho...
A simple, economical, precise and accurate method for simultaneous determination of Dutasteride (DUTA) and Tamsulosin (TAM) in combined dosage form has been developed. The first method is first order Derivative spectroscopy method (Method A) in which derivative amplitudes were measured at selected wavelengths. Second method is Area Under Curve Spec...
Two methods for the simultaneous determination of Emtricitabine and Tenofovir by spectroscopy have been developed. These two simple, accurate and precise methods include Area Under the Curve (AUC) method and Dual Wavelength Method. From a solvent effect studies and the spectral behaviours of Emtricitabine and Tenofovir, methanol was selected as sol...
Simple, accurate, precise, and sensitive spectrophotometric method for simultaneous estimation of Montelukast (MON) and Levocetirizine (LEV) in combined tablet dosage form have been developed and validated. The ratio derivative spectroscopic method involves measurement of first derivative amplitude of ratio spectra at 250.4 nm for MON and 238.4 nm...
Two methods for simultaneous determination of Emtricitabine and Tenofovir Disoproxil Fumarate by spectroscopy have been developed. First method is Simultaneous equation method and second method is Absorbance ratio Method. From a solvent effect studies and the spectral behaviours of Emtricitabine and Tenofovir Disoproxil Fumarate, methanol was selec...
A simple, precise, rapid and accurate RP-HPLC-PDA method has been developed for the simultaneous estimation of drotaverine HCl (DRT) and aceclofenac (ACE) in combined tablet dosage form. Formulation containing DRT with ACE are used as antispasmodic and anti-inflammatory agent. The chromatographic separation was achieved on Qualisil BDS C8 column (2...
A simple, economical, precise and accurate method for simultaneous determination of Atenolol (ATE) and Lercanidipine Hydrochloride (LER) in combined dosage form has been developed. The first method is based on Ratio Derivative (Method A) and second method is based on Dual Wavelength (Method B). The amplitudes 266.98 nm and 386.97 nm wavelength were...
A simple, sensitive, selective, precise and stability-indicating thin-layer chromatographic method for determination of dutasteride both as a bulk drug and as pharmaceutical tablets was developed and validated as per the International Conference on Harmonization guidelines. The method employed thin-layer chromatography aluminium plates precoated wi...
A simple, sensitive, selective, precise and stability indicating high-performance thin-layer chromatographic method was developed for the determination of tamsulosin (TAM) in bulk and tablet formulation. Validation was carried out in compliance with International Conference on Harmonization guidelines. The method employed thin-layer chromatography...
Simple, accurate, precise, and sensitive spectrophotometric method for simultaneous estimation of Tamsulosin(TAM) and Finasteride(FINA) in combined tablet dosage form have been developed and validated. The ratio derivative spectroscopic method involves measurement of first derivative amplitude of ratio spectra at 240.01nm for FINA and 229.91 nm for...
Two simple, precise and economical UV spectrophotometric methods have been developed for the estimation of Ropinirole in pharmaceutical dosage form. Method A applied was area under curve (AUC) in which area under curve was integrated in the wavelength range of 234.36 -241 nm. Method B involves getting first order derivative spectrum of drug solutio...
A simple, precise, rapid and accurate HPTLC method has been developed for the simultaneous estimation of Drotaverine Hydrochloride (DRO) and Diclofenac Potassium (DFK) in bulk and pharmaceutical dosage form on silica gel precoated aluminum 60F 254 plates, (20 cm x10 cm) with 250 µm thickness. The separation was carried out using Toluene: Ethyl acet...
Questions
Questions (3)
Dear Researchers,
I am planning antidiabetic study laboratory study using Wistar albino rats. Aim of the study is to evaluate antidiabetic potential, quality of life, herb-herb, herb-drug interaction of two polyherbal formulations present in the local market and metformin will be standard. We plan to study liver, kidney, heart, pancrea protection by these formaltions in addition to antihyperglycemic, antihyperlipidemic effect and effect on oxidative stress. OGTT will be performed before and after the study.
Need advise - should we perform insulin sensitivity and insulin resistance, both test in preclinical diabetic studies?
I am working on anti diabetic new drug, I seek guidelines whether I should carryout OGTT on normoglycemic or TI and TII DM animals or on both.
Thanks in advance
I am working on Insitu ophthalmic gel containing two drugs with molecular weight about 500 and 350. I need to decide the type of cellophane membrane.
