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Publications (11)
Cellulase enzymes serve as crucial tools in tackling the environmental issues caused due to lignocellulosic waste materials. These wastes comprising cellulose, hemicellulose, and lignin have traditionally led to environmental issues due to their improper disposal in urban areas. In this aspect, cellulase provides a sustainable solution to numerous...
Emerging pollutants (EPs) have been identified and quantified in living things and various environmental substances in recent years. Basically, chemicals that are either man-made or naturally occurring and have the potential to enter the environment and have a negative influence on both the ecosystem and human health are referred to as emerging pol...
This book chapter examines the evolving landscape of policies associated with emerging pollutants on a global scale. In light of the present scenario, where numerous unassessed emerging pollutants are widespread in the ecosystem, understanding existing policies and their shortcomings is imperative. By conducting an inclusive assessment of existing...
Rapid detection and identification of infectious pathogens in clinical samples is very challenging, and it is essential for proper treatment and predicts the outcome. To date, several notorious viruses had an impact on the health and social well-being across the globe in a short period. Bioanalytes like nucleic acids or proteins are usually the tar...
Isocoumarin is a lactone, a type of natural organic compound that is used as synthetic intermediates of several natural products and pharmaceutical compounds explored for their potential therapeutic applications like antifungal, antimicrobial, anti-inflammatory, and anticancer activities. In our previous work, we were the first group to report the...
Background
Isothiocyanates (ITCs) are small molecules having importance in synthetic organic chemistry, but their actual importance lies in their potential as anti-carcinogens. Through this piece of work an effort was made to assess the anti-cancer activity of some simple ITCs which can be synthesized through easy greener pathways.
Methods
Cell pr...
Epithelial–mesenchymal transition (EMT) is a key step in transdifferentiation process in solid cancer development. Forthcoming evidence suggest that the stratified program transforms polarized, immotile epithelial cells to migratory mesenchymal cells associated with enhancement of breast cancer stemness, metastasis, and drug resistance. It involves...
Background:
Colorectal cancer (CRC) is one of the leading cause of cancer deaths worldwide. Since CRC is largely asymptomatic until alarm features develop to advanced stages, the implementation of the screening programme is very much essential to reduce cancer incidence and mortality rates. CRC occurs predominantly from accumulation of genetic and...
Breast cancer is found to be the most prevalent neoplasm in women worldwide. Despite the function of physically tethering cells to the matrix, transmembrane protein integrins are crucially involved in diverse cellular functions such as cell differentiation, proliferation, invasion, migration and metastasis. Dysregulation of integrins and their inte...
Questions
Questions (2)
I performed indirect in cell-elisa using HRP cojugated secondary antibody, where OPD was used as peroxidase substrate. The substrate produces a soluble end product that is orange - brown in color which can be read spectrophotometrically at 450nm. Now I would like to do in cell- elisa using flurescent tagged secondary antibody. But I am unable to find good refernces to perform the experiment.
Once I tried by collecting the spheroids by centrifugation at low speed Aafter fixing with 4% paraformaldehyde the cells were permeabilized with triton x 100. Incubation of primary antibody was done overnight at 4 degree, followed by tagging it with alexa fluoro secondary antibody. Washed and mounted on slides. Dot like structures were observed @ 40x. No rigid spheroids were seen.
Kindly suggest some proper protocols.
