Violeta Trusca

Violeta Trusca
Nicolae Simionescu Institute of Cellular Biology and Pathology | ICBP · Genomics, Transcriptomics and Molecular Therapies

Ph.D.

About

28
Publications
2,385
Reads
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278
Citations
Introduction
Violeta Trusca currently works at the Genomics, Transcriptomics and Molecular Therapies, Institute of Cellular Biology and Pathology, Nicolae Simionescu. Violeta does research in Cell Biology and Molecular Biology. The current project is 'Apolipoproteins gene regulation and atherosclerosis.'
Additional affiliations
March 2011 - present
Nicolae Simionescu Institute of Cellular Biology and Pathology
Position
  • Researcher
October 2006 - February 2011
Nicolae Simionescu Institute of Cellular Biology and Pathology
Position
  • Research Assistant
Education
October 2008 - June 2013
Nicolae Simionescu Institute of Cellular Biology and Pathology
Field of study
  • Cellular and Molecular Biology
October 2006 - June 2008
Faculty of Biology, University of Bucharest
Field of study
  • Biochemistry and Molecular Biology
October 2002 - June 2006
Faculty of Biology, University of Bucharest
Field of study
  • Biochemistry

Publications

Publications (28)
Article
Apolipoprotein A-I (ApoAI) is an anti-atherosclerotic protein that promotes cholesterol efflux from tissues to the liver for excretion [1]. ApoAI is the main structural and functional protein of High- Density Lipoproteins (HDL), representing ~70% of total HDL proteins [2,3]. Circulating apoAI protein is an amphipathic protein (28kDa) comprising eig...
Article
Full-text available
Adenoviral vectors are important vehicles for delivering therapeutic genes into mammalian cells. However, the yield of the adenoviral transduction of murine mesenchymal stromal cells (MSC) is low. Here, we aimed to improve the adenoviral transduction efficiency of bone marrow-derived MSC. Our data showed that among all the potential transduction bo...
Article
Full-text available
Fas ligand (First apoptosis signal ligand, FasL, also known as CD95L) is the common executioner of apoptosis within the tumor necrosis factor (TNF) superfamily. We aimed to induce functional FasL expression in transduced cells using an adenovirus vector, which has the advantage of strong and transient induction of the gene included in the adenovira...
Article
Full-text available
Apolipoprotein A-I (apoA-I) is the major protein component of high-density lipoproteins (HDL), mediating many of its atheroprotective properties. Increasing data reveal the pro-atherogenic effects of bisphenol A (BPA), one of the most prevalent environmental chemicals. In this study, we investigated the mechanisms by which BPA exerts pro-atherogeni...
Article
The chitosan hydrochloride (Cs·HCl) was obtained as a polymer soluble in physiological solutions to be used as potential support for safely cell culture or cell encapsulation. Viability tests showed that concentrations between 0.16 and 5 mg/mL of Cs·HCl were not toxic for the HEK293 cells. In parallel, aldehyde-functionalized pullulan (Pul-CHO) was...
Article
Full-text available
Apolipoprotein E (apoE) is mainly secreted by hepatocytes and incorporated into most plasma lipoproteins. Macrophages, which accumulate cholesterol and are critical for the development of the atherosclerotic plaque, are also an important, albeit smaller, apoE source. Distal regulatory elements control cell-specific activity of the apoE promoter: mu...
Conference Paper
Background Our previous results showed that Lis1 gene is preferentially expressed in CD133+ glioblastoma cells and suggested a role of Lis1 in maintaining the CD133+ glioblastoma cell population. It is also known that CD133+ cells are resistant against radiation therapy and chemotherapy. Therefore, we tested if the Lis1 gene silencing could increas...
Article
Full-text available
Apolipoprotein E (apoE), a 34kDa glycoprotein, mediates hepatic and extrahepatic uptake of plasma lipoproteins and cholesterol efflux from lipid-laden macrophages. In humans, three structurally different apoE isoforms occur, with subsequent functional changes and pathological consequences. Here, we review data supporting the involvement of apoE str...
Article
Full-text available
Lissencephaly-1 (Lis1) protein is a dynein-binding protein involved in neural stem cell division, morphogenesis and motility. To determine whether Lis1 is a key factor in glioblastoma, we evaluated its expression and function in CD133+ glioblastoma cells. Global, Lis1 gene expression is similar in glioblastoma and normal samples. Interestingly, imm...
Article
Full-text available
Apolipoprotein E (apoE) has anti-atherosclerotic properties, being involved in the transport and clearance of cholesterol-rich lipoproteins as well as in cholesterol efflux from cells. We hypothesized that glucocorticoids may exert anti-inflammatory properties by increasing the level of macrophage-derived apoE. Our data showed that glucocorticoids...
Article
Introduction Recent evidences show a key role for Lis1 in hematopoietic stem cells function and leukemogenesis by regulating the asymmetric division and self-renewal of stem cell population. Moreover, Lis1 is involved in neural progenitor cell migration. Therefore, we investigated Lis1 expression in CD133+ glioblastoma cells and evaluated its possi...
Article
Full-text available
Aim: To investigate the effect of high homocysteine (Hcy) levels on apolipoprotein E (apoE) expression and the signaling pathways involved in this gene regulation. Methods: Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot were used to assess apoE expression in cells treated with various concentrations (50-500 μmol/L) of...
Article
Full-text available
Atherosclerosis and its complications represent the leading death cause worldwide, despite many therapeutic developments. Atherosclerosis is a complex, multi-stage disease whereby perturbed lipid metabolism leads to cholesterol accumulation into the vascular walls and plaque formation. Generation of apoE-/- and LDLR-/- atherosclerosis mouse models...
Article
Full-text available
Apolipoprotein CII (apoCII) is a specific activator of lipoprotein lipase and plays an important role in triglyceride metabolism. The aim of our work was to elucidate the regulatory mechanisms involved in apoCII gene modulation in macrophages. Using Chromosome Conformation Capture we demonstrated that multienhancer 2 (ME.2) physically interacts wit...
Article
Controlled-diameter TiO(2) nanotubes were obtained by electrochemical anodizing of two different substrates (Ti and Ti6Al7Nb) in an aqueous electrolyte. As-formed TiO(2) nanotubes are amorphous and by subjecting to thermal treatments, the structure becomes crystalline. An optimal thermal treatment with a specific anatase/rutile ratio was chosen, de...
Article
Full-text available
In atherogenesis, macrophage-derived apolipoprotein E (apoE) has an athero-protective role by a mechanism that is not fully understood. We investigated the regulatory mechanisms involved in the modulation of apoE expression in macrophages. The experiments showed that the promoters of all genes of the apoE/apoCI/apoCIV/apoCII gene cluster are enhanc...
Conference Paper
Full-text available
Purpose: The aim of this study is to obtain cells expressing a chimeric molecule containing human apolipoprotein E (apoE) linked to a molecule of horse radish peroxidase (HRP), that function as a reporter gene. Methods: To clone the chimeric molecule apoE-HRP, standard molecular biology techniques (PCR, RT-PCR, enzymatic restriction of DNA, ligatio...

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