Vincenzo Brancaleone

Vincenzo Brancaleone
Università degli Studi della Basilicata | UniBas · Department of Sciences

PhD

About

85
Publications
9,006
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
3,374
Citations
Additional affiliations
March 2012 - present
Università degli Studi della Basilicata
Position
  • Pharmacology and Toxicology
October 2011 - present
Università degli Studi della Basilicata
Position
  • Professor (Assistant)
January 2011 - present
William Harvey Research Institute, Queen Mary University of London
Position
  • William Harvey Research Institute
Description
  • Research project collaboration

Publications

Publications (85)
Chapter
This chapter focuses on the role played by the H 2 S pathway in the regulation of blood pressure (BP). Hypertension is a multifactorial disease leading to fatal events associated with long‐term arterial remodeling and dysfunction. In addition, changes in both vascular structure and function strongly affect the homeostatic control of BP paving the w...
Article
Full-text available
It is now well established that the urothelium does not act as a passive barrier but contributes to bladder homeostasis by releasing several signaling molecules in response to physiological and chemical stimuli. Here, we investigated the potential contribution of the hydrogen sulfide (H2S) pathway in regulating human urothelium function in β3 adren...
Article
In the context of inflammation and immunity, there are fragmented and observational studies relating to the pharmacological activity of Mangifera indica L. and its main active component, mangiferin. Therefore, we aimed to analyze the potential beneficial effects of this plant extract (MIE, 90% in mangiferin) in a mouse model of gouty arthritis, to...
Article
The role of H2S in urothelial carcinoma (UC) is still unclear. Here we have evaluated the expression of H2S producing enzymes as well as the effect of endogenous and exogenous H2S on human bladder UC cells. In human UC cells the expression of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MS...
Preprint
Background and Purpose: In the context of inflammation and immunity, there are fragmented and observational studies relating to the pharmacological activity of Mangifera indica L. and its main active component mangiferin. We, therefore, aimed to evaluate the potential beneficial effects of this plant extract (MIE, 90% in mangiferin) in a mouse mode...
Article
Full-text available
Background and Purpose N-acylethanolamine acid amidase (NAAA) is a lysosomal enzyme accountable for the breakdown of N-acylethanolamides (NAEs) and its pharmacological inhibition determines beneficial effects in inflammatory conditions. The knowledge of NAAA in cancer is fragmentary with an unclarified mechanism whereas its contribution to colorect...
Article
Full-text available
Making gender bias visible allows to fill the gaps in knowledge and understand health records and risks of women and men. The coronavirus disease 2019 (COVID-19) pandemic has shown a clear gender difference in health outcomes. The more severe symptoms and higher mortality in men as compared to women are likely due to sex and age differences in immu...
Article
Full-text available
Clodronate is a bisphosphonate agent commonly used as anti-osteoporotic drug. Throughout its use, additional anti-inflammatory and analgesic properties have been reported, although the benefits described in the literature could not solely relate to their inhibition of bone resorption. Thus, the purpose of our in vitro study is to investigate whethe...
Article
Full-text available
Preservation of vascular wall integrity against degenerative processes associated with ageing, fat-rich diet and metabolic diseases is a timely therapeutical challenge. The loss of endothelial function and integrity leads to cardiovascular diseases and multiorgan inflammation. The protective effects of the H2S-donor erucin, an isothiocyanate purifi...
Article
Full-text available
Endothelial mesenchymal transition (EndMT) has been described as a fundamental process during embryogenesis; however, it can occur also in adult age, underlying pathological events, including fibrosis. Indeed, during EndMT, the endothelial cells lose their specific markers, such as vascular endothelial cadherin (VE-cadherin), and acquire a mesenchy...
Article
Full-text available
Phenolic compounds of red wine powder (RWP) extracted from the Italian red wine Aglianico del Vulture have been investigated for the potential immunomodulatory and anti-inflammatory capacity on human macrophages. These compounds reduce the secretion of IL-1β, IL-6, and TNF-α proinflammatory cytokines and increase the release of IL-10 anti-inflammat...
Article
Full-text available
Background and purpose: L-Cysteine or hydrogen sulfide (H2 S) donors induce a biphasic effect on pre-contracted isolated vessels. The contractile effect is displayed within a concentration range of 10nM-3μM followed by vasodilatation at 30-100μM. Here, we have investigated the signalling involved in H2 S-induced contraction. Experimental approach...
Article
Full-text available
Palmitoylethanolamide (PEA) is an endogenous fatty acid amide related to the endocannabinoid anandamide. PEA exerts intestinal anti-inflammatory effects, but knowledge of its role in colon carcinogenesis is still largely fragmentary. We deepened this aspect by studying the effects of PEA (ultramicronized PEA, um-PEA) on colon cancer cell proliferat...
Article
Since coronavirus disease 2019 (COVID-19) started as a fast-spreading pandemic, causing a huge number of deaths worldwide, several therapeutic options have been tested to counteract or reduce the clinical symptoms of patients infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific drugs for COVID-19 ar...
Article
Full-text available
COVID‐19, the illness caused by SARS‐CoV‐2, has a wide‐ranging clinical spectrum that, in the worst‐case scenario, involves a rapid progression to severe acute respiratory syndrome and even death. Epidemiological data show that obesity and diabetes are among the main risk factors associated with high morbidity and mortality. The increased susceptib...
Article
Full-text available
The COVID‐19 pandemic caused by new betacoronavirus SARS‐Cov‐2 demands rapid exploration of safe and effective therapeutic options. In this perspective, “drug repurposing” aims to represent a rapid and valuable strategy. In the last decades, the endogenous gasotransmitter hydrogen sulfide (H2S) has emerged as a ubiquitous modulator of several biolo...
Article
Full-text available
Background and purpose: A critical role for sphingosine kinase/sphingosine-1-phosphate pathway in the control of airway function has been demonstrated in respiratory diseases. Here, we address sphingosine-1-phosphate contribution in a mouse model of mild chronic obstructive pulmonary disease (COPD). Experimental approach: C57BL/6J mice have been...
Article
Nonalcoholic steatohepatitis (NASH) is associated with an increased risk of developing cardiovascular complications and mortality, suggesting that treatment of NASH might benefit from combined approaches that target the liver and the cardiovascular components of NASH. Using genetic and pharmacologic approaches, we show that G protein-coupled bile a...
Article
Hydrogen sulfide (H2S) is produced by the action of cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) or 3-mercaptopyruvate sulfurtransferase (3-MST). 3-MST converts 3-mercaptopyruvate (MPT) to H2S and pyruvate. H2S is recognized as an endogenous gaseous mediator with multiple regulatory roles in mammalian cells and organisms. In the pres...
Article
Compelling evidence suggests the involvement of sphingosine-1-phosphate (S1P) in the pathogenesis of asthma. The systemic administration of S1P causes asthma like features in the mouse involving mast cells. In this study we investigated whether disodium cromoglycate (DSCG), administered as a preventative treatment as in human therapy, could affect...
Article
Background and purpose: The majority of the severe vascular complications in fibrosis is a consequence of a deregulated activity of mediators controlling vasomotor tone and endothelin-1 is known to play a major role. The aim of this paper was to investigate on the role of proteinase-activated receptor 2 (PAR-2) in vascular dysfunction occurring in...
Article
Full-text available
Aims: The molecular mechanism through which dipeptidyl peptidase 4 inhibitors (DPP4i) exert their beneficial effects on cardiovascular function is still unclear. Since DPP4i are not effective on type 1 diabetes (T1DM), we tested the effect of Linagliptin in Non Obese Diabetic (NOD) mice, a murine model of T1DM, to unveil a possible direct cardiova...
Article
Sildenafil, a selective phosphodiesterase type 5 (PDE5) inhibitor, commonly used in the oral treatment for erectile dysfunction, relaxes smooth muscle of human bladder through the activation of hydrogen sulfide (H2S) signaling. H2S is an endogenous gaseous transmitter with myorelaxant properties predominantly formed from L-cysteine (L-Cys) by cysta...
Article
Background and purpose: Hydrogen sulfide (H2 S) is a gasotransmitter produced from L-cysteine through enzymatic action by cystathionine-γ-lyase (CSE) and/or cystathionine-β-synthase (CBS). D-penicillamine (D-pen) is the D isomer of a dimethylated cysteine and it has been used for the treatment of rheumatoid arthritis (RA). Given to the close chemi...
Article
Glucocorticoid (GC)-induced hypertension is a common clinical problem still poorly understood. The presence of GC receptor (GR) in vascular smooth muscle and endothelial cells suggest a direct role for GC in vasculature. In response to hemodynamic shear stress, endothelium tonically releases nitric oxide (NO), endothelial-derived hyperpolarizing fa...
Article
Introduction: Glucocorticoid (GC) excess is related to hypertension. The deletion of endothelial GC-receptors abrogates the blood pressure increase, suggesting GC-induced hypertension is endothelium-dependent. In response to shear stress endothelium releases nitric oxide, endothelial derived hyperpolarizing factor (EDHF) and prostacyclin. Recently...
Article
Full-text available
Hydrogen sulfide (H2S) is a gaseous mediator synthesized in mammalian tissues by three main enzymes, cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate-sulfurtransferase (3MST), and its levels increase under inflammatory conditions or sepsis. Since H2S and H2S-releasing molecules afford inhibitory properties on leuko...
Article
Background and purpose: Hydrogen sulphide (H2S) is a gaseous mediator strongly involved in cardiovascular homeostasis, where it provokes vasodilatation. Having previously shown that H2 S contributes to testosterone-induced vasorelaxation, here we aim to uncover the mechanisms underlying this effect. Experimental approach: H2 S biosynthesis was e...
Article
Full-text available
Therapeutic use of sulfhydrylated inhibitor S-zofenopril has raised different hypotheses regarding the role played by its thiol group in the beneficial clinical effects exerted compared to other ACE inhibitors. Here, we investigated on H2S pathway as the accountable for extra-beneficial effects in vascular function.Methods & ResultsSHR and WKY rats...
Article
Full-text available
Sphingosine-1-phosphate (S1P) represents one of the key latest additions to the list of vasoactive substances that modulate vascular tone. Proteinase activated receptor-2 (PAR-2) has been shown to be involved in cardiovascular function. In the present study we investigated the involvement of PAR-2 in S1P-induced effect on vascular tone. This study...
Article
Full-text available
Neutrophil activation and adhesion must be tightly controlled to prevent complications associated with excessive inflammatory responses. The role of the anti-inflammatory peptide chemerin15 (C15) and the receptor ChemR23 in neutrophil physiology is unknown. Here, we report that ChemR23 is expressed in neutrophil granules and rapidly upregulated upo...
Article
Hydrogen sulfide (H2S), a gaseous mediator synthesized in several mammalian tissues by two main enzymes CBS and CSE, increases under inflammatory conditions or sepsis [1,2]. Inflammation per se does not represent an unwanted event, since it also provides a physiological response to an injury, keeping inflammation under control [3]. Annexin A1 (AnxA...
Article
Full-text available
Endogenous protective pathways mitigate the overshooting of inflammation after sterile or infectious injury. Here we report that formyl peptide receptor 2 (Fpr2/3) null mice display a major phenotype with exacerbated vascular inflammation observed postischemia reperfusion (IR) injury of the mesenteric artery, characterized by marked neutrophil adhe...
Data
The Supplementary Data contains material, which casts further light on the immune response effects of treatment with HOCbl, and/or the thiol Cbls, during in vivo, experimental endotoxaemia. This material comprises blood analyses of circulating neutrophils/PMN, and of the adhesion molecule/complement receptor, CD11b, a marker of neutrophil activatio...
Data
Background. NOS/ NO inhibitors are potential therapeutics for sepsis, yet they increase clinical mortality. However, there has been no in vivo investigation of the (in vitro) NO scavenger, cobalamin's (Cbl) endogenous eects on NOS/ NO/inammatory mediators during the immune response to sepsis. Methods. We used quantitative polymerase chain reaction...
Data
Background. NOS/ NO inhibitors are potential therapeutics for sepsis, yet they increase clinical mortality. However, there has been no in vivo investigation of the (in vitro) NO scavenger, cobalamin's (Cbl) endogenous eects on NOS/ NO/inammatory mediators during the immune response to sepsis. Methods. We used quantitative polymerase chain reaction...
Article
Full-text available
Background. NOS/•NO inhibitors are potential therapeutics for sepsis, yet they increase clinical mortality. However, there has been no in vivo investigation of the (in vitro) •NO scavenger, cobalamin's (Cbl) endogenous effects on NOS/•NO/inflammatory mediators during the immune response to sepsis. Methods. We used quantitative polymerase chain reac...
Article
Background and purpose: Proteinase-activated receptors (PARs) and toll-like receptors (TLRs) are involved in innate immune responses. The aim of this study was to evaluate the possible cross-talk between PAR(2) and TLR4 in vessels in physiological condition and how it varies following stimulation of TLR4 by using in vivo and ex vivo models. Exper...
Conference Paper
Full-text available
Background Hydrogen sulphide (H2S) is a novel gaseous mediator enzymatically produced by cystathionine-beta-synthase (CBS) and cystathionine-gamma-lyase (CSE). It is involved in physiological homeostatic processes and several pathological conditions; however, more importantly, H2S has a prominent role in cardiovascular system, where CSE deletion re...
Article
Background The need for new drugs in melanoma treatment is of great relevance. Indeed, current therapies for the treatment of metastatic melanoma offer a limited clinical benefit and only in recent years there has been an advancement due to the identification of new molecular targets [1]. Hydrogen sulphide (H2S) is endogenously produced by the acti...
Article
Full-text available
Understanding how proresolving agonists selectively activate FPR2/ALX is a crucial step in the clarification of proresolution molecular networks that can be harnessed for the design of novel therapeutics for inflammatory disease. FPR2/ALX, a G protein-coupled receptor belonging to the formyl peptide receptor (FPR) family, conveys the biological fun...
Article
Agrin is over-expressed by activated and autoimmune T cells, and synergizes with the T cell receptor (TCR) to augment cell activation. In the present study, we show that Agrin accumulates to distinct areas of the plasma membrane and that cell activation causes its redistribution. During antigen presentation, Agrin primarily accumulates to the perip...
Article
The anti-inflammatory properties of the female sex hormone estrogen have been linked to a reduced incidence of cardiovascular disease. In the present study, we addressed whether estrogen could activate vasculoprotective mechanisms via annexin A1 (AnxA1) mobilization in human polymorphonuclear cells (PMNs). Using whole-blood flow cytometry, we demon...
Article
Full-text available
The importance of proresolving mediators in the overall context of the resolution of acute inflammation is well recognized, although little is known about whether these anti-inflammatory and proresolving molecules act in concert. In this article, we focused on lipoxin A(4) (LXA(4)) and annexin A1 (AnxA1) because these two very different mediators c...
Article
Full-text available
The sphingosine kinase (SPK)/sphingosine-1-phosphate (S1P) pathway recently has been associated with a variety of inflammatory-based diseases. The majority of these studies have been performed in vitro. Here, we have addressed the relevance of the SPK/S1P pathway in the acute inflammatory response in vivo by using different well known preclinical a...
Article
Recent studies have demonstrated that hydrogen sulfide (H(2)S) is produced within the vessel wall from L-cysteine regulating several aspects of vascular homeostasis. H(2)S generated from cystathione γ-lyase (CSE) contributes to vascular tone; however, the molecular mechanisms underlying the vasorelaxing effects of H(2)S are still under investigatio...
Data
Full-text available
Ac2-26 affected genes. Differentially expressed genes (FC greater than or equal to 50%) in HEK293 cells transfected with ALX receptor and treated with 0.5 uM Ac2-26 peptide during 4 hours. (0.04 MB PDF)
Data
Full-text available
AnxA1 affected genes. Differentially expressed genes (FC greater than or equal to 50%) in HEK293 cells transfected with ALX receptor and treated with 10 uM AnxA1 peptide during 4 hours. (0.04 MB PDF)
Article
Full-text available
Our understanding of pro-resolution factors in determining the outcome of inflammation has recently gained ground, yet not many studies have investigated whether specific genes or patterns of genes, are modified by these mediators. Here, we have focussed on the glucocorticoid modulated pro-resolution factor annexin A1 (AnxA1), studying if its inter...