
Vincent LaizéCentro de Ciências do Mar | CCMAR
Vincent Laizé
PhD in Molecular Biophysics
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163
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October 2005 - September 2015
Publications
Publications (163)
Spermatogonia cryopreservation is a method to preserve valuable genomes from both maternal and paternal origin. The damage associated with the application of this technology on post-thaw cell quality is important to assess, including at the epigenetic level. This study aimed to assess post-thawed spermatogonia quality by evaluating alterations in p...
The ectoparasite Amyloodinium ocellatum is a dinoflagellate that causes severe morbidity and mortality in both brackish and marine warmwater aquaculture fishes worldwide. A. ocellatum has a triphasic life cycle based on a free-living flagellate (the dinospore), a parasitic stage (the trophont) and a resting and reproductive cyst (the tomont). Curre...
Metabolic bone disorders and associated fragility fractures are major causes of disability and mortality worldwide and place an important financial burden on the global health systems. These disorders result from an unbalance between bone anabolic and resorptive processes and are characterized by different pathophysiological mechanisms. Drugs are a...
Skeletal disorders are problematic aspects for the aquaculture industry as skeletal deformities, which affect most species of farmed fish, increase production costs and affect fish welfare. Following recent findings that show the presence of osteoactive compounds in marine organisms, we evaluated the osteogenic and mineralogenic potential of commer...
Spermatogonia are increasingly used in biotechnological innovation related to transplantation in endangered or commercially
cultured fish species and to cryopreserve genetic material. In some species, spermatogonia enrichment and in vitro cultivation
are crucial steps to obtain enough of these germ cells. Senegalese sole (Solea senegalensis) is a f...
Ectopic calcification refers to the pathological accumulation of calcium ions in soft tissues and is often the result of a dysregulated action or disrupted function of proteins involved in extracellular matrix mineralization. While the mouse has traditionally been the go-to model organism for the study of pathologies associated with abnormal calciu...
The extensive use of plant ingredients in novel aquafeeds have introduced mycotoxins to the farming of seafood. The emerging enniatin B (ENNB) and beauvericin (BEA) mycotoxins have been found in the novel aquafeeds and farmed fish. Little is known about the potential toxicity of ENNs and BEA in farmed fish and their feed-to-organ transfer. Atlantic...
The shaping of bone structures relies on various cell types and signaling pathways. Here, we use the zebrafish bifurcating fin rays during regeneration to investigate bone patterning. We found that the regenerating fin rays form via two mineralization fronts that undergo an osteoblast-dependent fusion/stitching until the branchpoint, and that bifur...
Spermatogonia are increasingly used in biotechnological innovation related to
transplantation in endangered or commercially cultured fish species. However, in some
species spermatogonia enrichment is a crucial step in the success for further transplantations
or cryopreservation techniques. The aim of this study was to develop an efficient protocol...
Bone disorders affect millions of people worldwide and treatments currently available often produce undesirable secondary effects or have limited efficacy. It is therefore of the utmost interest for patients to develop more efficient drugs with reduced off-target activities. In the long process of drug development, screening and preclinical validat...
The dinoflagellate Amyloodinium ocellatum is the etiological agent of a parasitic disease named amyloodiniosis. Mortalities of diseased fish are usually attributed to anoxia, osmoregulatory impairment, or opportunistic bacterial infections. Nevertheless, the phylogenetic proximity of A. ocellatum to a group of toxin-producing dinoflagellates from P...
The presence of microplastics in the aquatic ecosystem represents a major issue for the environment and human health. The capacity of organic pollutants to adsorb onto microplastic particles raises additional concerns, as it creates a new route for toxic compounds to enter the food web. Current knowledge on the impact of pristine and/or contaminate...
The shaping of bone structures relies on various cell types and signalling pathways. Here, we use the zebrafish bifurcating fin rays during regeneration to investigate bone patterning. We found that the regenerating fin rays form via two mineralization fronts that undergo an osteoblast-dependent fusion/stitching until the branchpoint, and that bifu...
The marine habitat and its biodiversity can be impacted by released pharmaceuticals. The short-term (7 days) effect of 3 commonly used drugs – warfarin, dexamethasone and imidazole – on Senegalese sole (Solea senegalensis) juveniles was investigated. Occurrence of hemorrhages, histopathological alterations, antioxidant status, activity of antioxida...
Sixty years after the development of the first piscine cell line, more than 700 cell lines have been established from a wide range of fish species and tissues. The fast provision of in vitro cell systems of fish origin has been stimulated by the growing interest for fish in science and sustained by the ability of cultured fish cells to spontaneousl...
Persistent and ubiquitous organic pollutants, such as the polycyclic aromatic hydrocarbon benzo[⍺]pyrene (BaP), represent a major threat to aquatic organisms and human health. Beside some well-documented adverse effects on the development and reproduction of aquatic organisms, BaP was recently shown to affect fish bone formation and skeletal develo...
The BIOSKEL Lab (CCMAR, UALG) is pleased to announce the 3rd WORKSHOP on Zebrafish tools for the screening of osteogenic compounds that will be held at the CCMAR (Faro, PT) from 8 to 10th of September 2021. This workshop aims to provide both conceptual and experimental know-how on zebrafish screening systems through lectures given by experts in the...
Osteoporosis is an aging-related disease and a worldwide health issue. Current therapeutics have failed to reduce the prevalence of osteoporosis in the human population, thus the discovery of compounds with bone anabolic properties that could be the basis of next generation drugs is a priority. Marine plants contain a wide range of bioactive compou...
Dual specificity phosphatase 4 (DUSP4), a member of the dual specificity phosphatase family, is responsible for the dephosphorylation and inactivation of ERK, JNK and p38, which are mitogen-activated protein kinases involved in cell proliferation, differentiation and apoptosis, but also in inflammation processes. Given its importance for cellular s...
Osteopenia and osteoporosis are bone disorders characterized by reduced bone mineral density (BMD), altered bone microarchitecture and increased bone fragility. Because of global aging, their incidence is rapidly increasing worldwide and novel treatments that would be more efficient at preventing disease progression and at reducing the risk of bone...
Keutel syndrome (KS) is a rare autosomal recessive genetic disorder that was first identified in the beginning of the 1970s and nearly 30 years later attributed to loss-of-function mutations in the gene coding for the matrix Gla protein (MGP). Patients with KS are usually diagnosed during childhood (early onset of the disease), and the major traits...
Background/Introduction: Low bone mass density associated
disorders, such as osteoporosis, affect millions of people worldwide and
result in a massive economic burden for society. Current therapeutics are
limited and have issues related to costs, efficacy and long-term use.
Although still largely unexplored, marine biodiversity represents a
promisi...
Pharmaceuticals represent a group of emerging contaminants. The short-term effect (3 and 7 days) of warfarin (1 and 10 mg L⁻¹), dexamethasone (0.392 and 3.92 mg L⁻¹) and imidazole (0.013 and 0.13 mg L⁻¹) exposure was evaluated on mussels (Mytilus galloprovincialis). Total antioxidant status, glutathione reductase, glutathione peroxidase (GPx) and s...
Bone disorders such as osteoporosis and Paget´s disease of bone affect millions of people worldwide and available therapeutics have a limited efficacy and often present undesirable side effects. Furthermore, there is a plethora of rare diseases, such as osteopetrosis or osteogenesis imperfecta, that also present severe skeletal phenotypes and for w...
Fish cultured in intensive farming conditions commonly develop severe skeletal deformities that affect both welfare and market value. Among the solutions proposed to improve the skeletal status of aquaculture fish, supplementation of their diet with natural compounds or extracts that stimulate skeletogenesis is increasingly seen as an economically...
Metabolic bone disorders such as osteoporosis affect millions of people worldwide and drugs currently available have a limited efficacy and have associated secondary effects. There is a compelling need for new compounds with bone anabolic properties acting through new and original mechanisms of action. In this regard, recent works have identified m...
Aim: To provide novel data on the expression of DUSP4 transcripts in colorectal cancer (CRC) tissues and to explore their potential as biomarkers. Materials & methods: DUSP4 transcripts expression was determined by quantitative real-time PCR in tissues from 28 CRC patients. Their association with clinicopathological factors and survival analysis wa...
Teleosts have the ability to regenerate their caudal fin upon amputation. A highly proliferative mass of undifferentiated cells called blastema forms beneath wound epidermis and differentiates to regenerate all missing parts of the fin. To date, the origin and fate of the blastema is not completely understood. However, current hypotheses suggest th...
The last decade has seen an increased interest in the discovery of compounds with bone anabolic activity to treat skeletal disorders such as osteoporosis and increase the well-being of patients. Due to the many technical advantages over classical rodent systems, zebrafish (Danio rerio) has been increasingly used in screening pipelines, in particula...
Vitamin K (VK) is a key nutrient for several biological processes (e.g., blood clotting and bone metabolism). To fulfill VK nutritional requirements, VK action as an activator of pregnane X receptor (Pxr) signaling pathway, and as a co-factor of γ-glutamyl carboxylase enzyme, should be considered. In this regard, VK recycling through vitamin K epox...
The caudal fin of teleost fish has become an excellent system for investigating the mechanisms of epimorphic regeneration. Upon amputation of the caudal fin, a mass of undifferentiated cells, called blastema, proliferate beneath the wound-epidermis and differentiate into various cell types to faithfully restore the missing fin structures. Here we d...
Many anthropogenic chemicals and plastic debris end up in the aquatic ecosystem worldwide, representing a major concern for the environment and human health. Small teleosts, such as zebrafish (Danio rerio) and Japanese medaka (Oryzias latipes), offer significant advantages over classical animal models and are currently used as first-line organisms...
Trace minerals and vitamins are known modulators of bone metabolism, and dietary optimization of these components may improve skeletal development and reduce the occurrence of skeleton deformities in farmed fish. As for larval stages, mineral and water-soluble vitamin nutrition requirements are lacking in research efforts and knowledge is scarce. A...
Warfarin is the most worldwide used anticoagulant drug and rodenticide. Since it crosses placental barrier it can induce warfarin embryopathy (WE), a fetal mortality in neonates characterized by skeletal deformities in addition to brain hemorrhages. Although the effects of warfarin exposure in aquatic off target species were already described, the...
[This corrects the article DOI: 10.1371/journal.pone.0207303.].
Extracts and compounds derived from marine organisms have reportedly shown some osteogenic potential. As such, these bioactives may aid in the treatment of musculoskeletal conditions such as osteoporosis; helping to address inefficacies with current treatment options. In this study, 72 fractions were tested for their in vitro osteogenic activity us...
hFOB proliferation with DMSO dissolved extract set 1.
Proliferation of hFOBs at day 4 (a) and 7 (b) calculated via PicoGreen assay. Cells were challenged with DMSO dissolved extracts D-01 to D-14. DMSO was included at 0.1%, giving extract concentrations ranging between 20 and 31.7 μg/ml. Cell proliferation, as a percentage of control, is presented...
hFOB proliferation with DMSO dissolved extract set 3.
Proliferation of hFOBs at day 4 (a) and 7 (b) calculated via PicoGreen assay. Cells were challenged with DMSO dissolved extracts D-29 to D-43. DMSO was included at 0.1%, giving extract concentrations ranging between 20 and 31.7 μg/ml. Cell proliferation, as a percentage of control, is presented...
Dataset providing all raw values used to create figures in this manuscript.
Datasets are labelled according to the extracts tested, the type of data (i.e. in vitro or in vivo), time-point used and which figure the data relates to.
(XLSX)
hFOB proliferation with DMSO dissolved extract set 2.
Proliferation of hFOBs at day 4 (a) and 7 (b) calculated via PicoGreen assay. Cells were challenged with DMSO dissolved extracts D-15 to D-28. DMSO was included at 0.1%, giving extract concentrations ranging between 20 and 31.7 μg/ml. Cell proliferation, as a percentage of control, is presented...
hFOB cytotoxicity results for DMSO dissolved extracts.
Cytotoxicity results (LDH assay) for hFOBs at day 1. Cells were challenged with DMSO dissolved extracts D-01 to D-14 (a), D-15 to D-28 (b) and D-29 to D-43 (c). DMSO was included at 0.1%, giving extract concentrations ranging between 20 and 31.7 μg/ml. Cell death is presented as mean +/- SD (n...
hFOB proliferation results for ethanol dissolved extract set 1.
Proliferation of hFOBs at day 4 (a) and 7 (b) calculated via crystal violet assay. Cells were challenged with ethanol dissolved extracts E-01 to E-13 or control (EtOH). Ethanol was included at a 0.5% concentration, giving extract concentrations of 150 μg/ml. Cell proliferation, as a pe...
hFOB proliferation results for ethanol dissolved extract set 2.
Proliferation of hFOBs at day 4 (a) and 7 (b) calculated via crystal violet assay. Cells were challenged with ethanol dissolved extracts E-14 to E-25 or control (EtOH). Ethanol was included at a 0.5% concentration, giving extract concentrations of 150 μg/ml (except for E-19: 20.8 μg/ml...
hFOB cytotoxicity results for ethanol dissolved extracts.
Cytotoxicity results (LDH assay) for hFOBs at day 1. Cells were challenged with EtOH dissolved fractions E-01—E13 (a) and E-14—E-25 (b). Ethanol was included at a 0.5% concentration, giving extract concentrations of 150 μg/ml (except for E-19: 20.8 μg/ml and E-22: 86 μg/ml). Cell death is pr...
Many chemicals produced by human activities end up in the aquatic ecosystem causing adverse developmental and reproductive effects in aquatic organisms. There is evidence that some anthropogenic chemicals disturb bone formation and skeletal development but the lack of suitable in vitro and in vivo systems for testing has hindered the identification...
Metabolic programming refers to the induction, deletion, or impaired development of a somatic structure or “setting” of a physiological system by an early life stimulus operated at a critical period during development. Ghrelin is the only known orexigenic gut hormone, is an acylated peptide that acts as an endogenous ligand specific for growth-horm...
Through the current trend for bioprospecting, marine organisms - particularly algae - are becoming increasingly known for their osteogenic potential. Such organisms may provide novel treatment options for osteoporosis and other musculoskeletal conditions, helping to address their large healthcare burden and the limitations of current therapies. In...
Vertebrate tissues are plastic and actively adapt to the mechanical environment. Teleost bone, despite differences with its mammalian counterpart, also responds to mechanical loading, evidencing the presence of a functional mechanosensing system. Deformities such as spinal curvatures and vertebral compressions can result in internal loading disturb...
Although human and mouse genetics have largely contributed to the better understanding of the mechanisms underlying skeletogenesis, much more remains to be uncovered. In this regard alternative and complementary systems have been sought and cell systems capable of in vitro calcification have been developed to study the mechanisms underlying bone fo...
Environmental toxicology has been expanding as growing concerns on the impact of produced and released chemical compounds over the environment and human health are being demonstrated. Among the toxic effects observed in organisms exposed to pollutants, those affecting skeletal tissues (osteotoxicity) have been somehow overlooked in comparison to he...
Skeletal disorders such as osteopenia and osteoporosis – that affect millions of people worldwide – result from defective or impaired bone formation and remodelling. In order to improve bone status, it is of uttermost importance to discover novel molecules with bone anabolic properties but also to gain insights into the complex mechanisms of bone f...
The pregnane X receptor (PXR) is a nuclear receptor belonging to the NR1i sub-family and a known master regulator of xenobiotic metabolism. New roles have been recently proposed in mammals through its activation by vitamin K (VK) such as regulation of glucose metabolism, bone homeostasis, reproduction, neuronal development and cognitive capacities....
Bone disorders affect millions of people worldwide and available therapeutics have a limited efficacy, often presenting undesirable side effects. As such, there is a need for novel molecules with bone anabolic properties. The aim of this work was to establish a rapid, reliable and reproducible method to screen for molecules with osteogenic activiti...
Marine macroalgae represent a valuable natural resource
for bioactive phytochemicals with promising applications
in therapeutics, although they remain largely under-exploited. In
this work, the potential of two marine green macroalgae
(Cladophora rupestris and Codium fragile) as a source of bioactive
phenolic compounds was explored, and antioxidant...
The ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the
mechanisms underlying de novo bone formation and to develop screening methods towards the
discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely
depends on successfully measuring new tissue formation through...
Background:
To better understand the complex mechanisms of bone formation it is fundamental that genes central to signalling/regulatory pathways and matrix formation are identified. Cell systems were used to analyse genes differentially expressed during extracellular matrix mineralization and bhmt3, coding for a betaine-homocysteine S-methyltransf...
MGP is a protein that was initially associated to inhibition of calcification in skeleton, soft tissues and arteries, but more recently also implicated in cancer. In breast cancer, higher levels of MGP mRNA were associated to poor prognosis but since this deregulation was never demonstrated at the protein level, we postulated the involvement of a p...
BMP2, BMP4 and BMP16 form a subfamily of bone morphogenetic proteins acting as pleiotropic growth factors during development and as bone inducers during osteogenesis. BMP16 is the most recent member of this subfamily and basic data regarding protein structure and function, and spatio-temporal gene expression is still scarce. In this work, insights...
Bone morphogenetic protein (BMP) 2 belongs to the transforming growth factor β (TGFβ) superfamily of cytokines and growth factors. While it plays important roles in embryo morphogenesis and organogenesis, BMP2 is also critical to bone and cartilage formation. Protein structure and function have been remarkably conserved throughout evolution and BMP...
The present study aims at identifying, among six mammalian and fish cell lines, a sensitive cell line whose in vitro median inhibitory concentration (IC50) better matches the in vivo short-term Sparus aurata median lethal concentration (LC50). IC50s and LC50 were assessed after exposure to the widely used fungicide azoxystrobin (AZX). Statistical r...
Vitamin K (VK) acts as a cofactor driving the biological activation of VK-dependent proteins and conferring calcium-binding properties to them. As a result, VK is converted into VK epoxide, which must be recycled by VK epoxide reductases (Vkors) before it can be reused. Although VK has been shown to play a central role in fish development, particul...
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Osteocalcin (OC or bone Gla protein, BGP) and matrix Gla protein (MGP) are two members of the growing family of vitamin K-dependent (VKD) proteins. They were the first VKD proteins found not to be involved in coagulation and synthesized outside the liver. Both proteins were isolated from bone although it is now known that only OC is synthesized by...
This review discusses the suitability and advantages of teleost fish for studying underlying mechanisms of normal and pathological development and mineralization of vertebrate skeleton, presents a selection of zebrafish mutants and transgenic lines modeling human skeletal diseases and highlights currently available fish systems for identifying and...
Warfarin-induced vitamin K (VK) recycling impairment is used worldwide as a rodenticide and human
thromboembolic prophylactic. Since VK metabolism/signaling pathways have been conserved
throughout vertebrate evolution, its release to the environment might impact on aquatic organisms.
Present study assessed the toxic effect of warfarin (0, 5, 25 and...
Bone morphogenetic proteins (BMPs) are involved in various physiological processes from early life stages throughout adulthood. Specific characteristics of BMPs have been used to define different subfamilies and BMP2/4 subfamily (composed of BMP2 and BMP4) has been linked to osteogenesis and skeleton development. BMP16 was recently identified as a...
A novel S100 calcium-binding protein has been recently identified in teleost fish. Its expression is restricted in vivo to chondrocytes of cartilaginous tissues undergoing endo/perichondral mineralization and its function has been associated in vitro with mechanisms of extracellular matrix mineralization. To get more insights into this mineralogeni...
The present invention relates to isolated polypeptides having organophosphorous hydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Biotechnological researches are nowadays focused on natural compounds showing promising bioactivities for specific therapeutic application and/or as surfactant on biomaterials in order to improve their biocompatibility and efficacy. Among natural compounds, phytochemicals have been found to present antioxidant and antibacterial capacities but also...
MicroRNAs (miRNAs) are important regulators of vertebrate development but their role during skeletogenesis remains unknown. In this regard, we investigated the mineralogenic activity of miR-20a, a miRNA associated with osteogenesis, in fish bone-derived cells. Expression of miR-20a was up-regulated during differentiation and its overexpression inhi...
Marine algae have been identified as an under-exploited resource, although they have long been recognized as valuable sources of diverse bioactive compounds. These phytochemicals have been found to present antioxidant capacities but also to show effects in osteoblast proliferation and differentiation. Extracts from two green algae, Cladophora rupes...
Seaweeds and halophytes have been identified as an under-exploited resource, although they have long been recognized as valuable sources of diverse bioactive compounds. Moreover, several phytochemicals have been recently found to present antioxidant capacities and to stimulate bone formation. Bone diseases, in particular osteoporosis, are considere...
Seaweeds and halophytes have been identified as an under-exploited resource, although they have long been recognized as valuable sources of diverse bioactive compounds. Moreover, several phytochemicals have been recently found to present antioxidant capacities and to stimulate bone formation. Bone diseases, in particular osteoporosis, are considere...
Retinoic acid (RA), the main active metabolite of vitamin A, regulates vertebrate morphogenesis through signaling pathways not yet fully understood. Such process involves the specific activation of retinoic acid and retinoid X receptors (RARs and RXRs), which are nuclear receptors of the steroid/thyroid hormone receptor superfamily. Teleost fish ar...
The occurrence of a second osteocalcin (OC2) has been reported in teleost fish, where it coexists with OC1 in some species. While it has been proposed that OC2 gene originated from OC1 through the fish whole-genome duplication event, little information is available on its molecular function and physiological role. The present study brings biologica...