Vincent Hervé

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Verified

Vincent verified their affiliation via an institutional email.

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• PhD
• Researcher at French National Institute for Agriculture, Food, and Environment (INRAE)

Arbuscular mycorrhizal fungi (AMF) play central roles in terrestrial ecosystems by interacting with both above and belowground communities as well as by influencing edaphic properties. The AMF communities associated with the roots of the fern Botrychium lunaria (Ophioglossaceae) were sampled in four transects at 2400 m a.s.l. in the Swiss Alps and...

“Higher” termites have been able to colonize all tropical and subtropical regions because of their ability to digest lignocellulose with the aid of their prokaryotic gut microbiota. Over the last decade, numerous studies based on 16S rRNA gene amplicon libraries have largely described both the taxonomy and structure of the prokaryotic communities a...

Background: Over the last decade, we have observed in microbial ecology a transition from gene-centric to genome-centric analyses. Indeed, the advent of metagenomics combined with binning methods, single-cell genome sequencing as well as high-throughput cultivation methods have contributed to the continuing and exponential increase of available pro...

Iron is involved in various microbial metabolisms and interactions and is an essential micronutrient for most microorganisms. This review focuses on the cheese ecosystem, in which iron is sparse (median concentration of 2.9 mg/kg based on a literature survey) and of limited bioavailability due to the presence of various metal-binding agents in the...

Background Estuaries are complex ecosystems linking river and marine environments, where microorganisms play a key role in maintaining ecosystem functions. In the present study, we investigated monthly 8 sites at two depth layers and over a one-year period the bacterial and eukaryotic community dynamics along the Seine macrotidal estuary (Normandy,...

Sessilids (Oligohymenophorea, Peritrichia, Sessilida) reportedly colonize the gut of certain “higher termites” (family Termitidae), but only a single species, Termitophrya africana from Jugositermes tuberculatus (subfamily Apicotermitinae), has been described based on a drawing. Similar ciliates were observed in other Apicotermitinae but remained u...

Iron is a vital micronutrient for nearly all microorganisms, serving as a co-factor in critical metabolic pathways. However, cheese is an iron-restricted environment. Furthermore, it has been demonstrated that iron represents a growth-limiting factor for many microorganisms involved in cheese ripening and that this element is central to many microb...

Cellulolytic flagellates are essential for the symbiotic digestion of lignocellulose in the gut of lower termites. Most species are associated with host‐specific consortia of bacterial symbionts from various phyla. 16S rRNA‐based diversity studies and taxon‐specific fluorescence in situ hybridization revealed a termite‐specific clade of Actinomycet...

Background The microbial landscape within termite guts varies across termite families. The gut microbiota of lower termites (LT) is dominated by cellulolytic flagellates that sequester wood particles in their digestive vacuoles, whereas in the flagellate-free higher termites (HT), cellulolytic activity has been attributed to fiber-associated bacter...

Recent metagenomic studies have identified numerous lineages of hydrogen-dependent, obligately methyl-reducing methanogens. Yet only a few representatives have been isolated in pure culture. Here, we describe six new species with this capability in the family Methanosarcinaceae (order Methanosarcinales), which makes up a substantial fraction of the...

Bacterial endosymbionts of eukaryotic hosts typically experience massive genome reduction, but the underlying evolutionary processes are often obscured by the lack of free-living relatives. Endomicrobia, a family-level lineage of host-associated bacteria in the phylum Elusimicrobiota that comprises both free-living representatives and endosymbionts...

Similar to many small islands within the Wider Caribbean Region, the French West Indies are rich but fragile island ecosystems threatened by global warming, pollutants and other anthropic pressures. To understand integrated and complex human impacts on the environment, the Caribbean Coast Human-Environment Observatory (OHM) is developing disciplina...

Methane emission by terrestrial invertebrates is restricted to millipedes, termites, cockroaches, and scarab beetles. The arthropod-associated archaea known to date belong to the orders Methanobacteriales, Methanomassiliicoccales, Methanomicrobiales, and Methanosarcinales, and in a few cases also to non-methanogenic Nitrososphaerales and Bathyarcha...

In Cambodia, termite mounds are commonly used by farmers as amendments to increase the fertility of their paddy fields. However, despite their utilization, their chemical and physical properties have not been described yet. Therefore, the aim of this study was to analyze the chemical and physical properties of two termite constructions commonly fou...

Land use change and anthropogenic forcing can drastically alter the rates and patterns of sediment transport and modify biodiversity and ecosystem functions in coastal transition zones, such as the coastal ecosystems. Molecular studies of sediment extracted DNAs provide information on currently living organisms within the upper layers or buried fro...

In the Lower Mekong Basin, paddy fields often appear as mosaics, with soil mounds covered by trees or other plants in a spotty distribution. These soil mounds are commonly named termite ‘lenticular mounds’ because termite bioturbation is considered to be at their origin. Termite mounds host a large diversity of animals and plants, increasing landsc...

Termites have co-evolved with a complex gut microbiota consisting mostly of exclusive resident taxa, but key forces sustaining this exclusive partnership are still poorly understood. The potential for primary reproductives to vertically transmit their gut microbiota (mycobiome and bacteriome) to offspring was investigated using colony foundations f...

Background: Over the last decade, we have observed in microbial ecology a transition from gene-centric to genome-centric analyses. Indeed, the advent of metagenomics combined with binning methods, single-cell genome sequencing as well as high-throughput cultivation methods have contributed to the continuing and exponential increase of available pro...

The digestive capacity of organic compounds by the black soldier fly (BSF, Hermetia illucens, Diptera: Stratiomyidae, Linnaeus, 1758) is known to rely on complex larva-microbiota interactions. Although insect development is known to be a driver of changes of bacterial communities, the fluctuations along BSF life cycle in terms of composition and di...

Most members of the family Treponemataceae (Spirochaetales) are associated with vertebrate hosts. However, a diverse clade of uncultured, putatively free-living treponemes comprising several genus-level lineages is present in other anoxic environments. The only cultivated representative to date is Treponema zuelzerae, isolated from freshwater mud....

Background Termites primarily feed on lignocellulose or soil in association with specific gut microbes. The functioning of the termite gut microbiota is partly understood in a handful of wood-feeding pest species but remains largely unknown in other taxa. We intend to fill this gap and provide a global understanding of the functional evolution of t...

Entombment, or the production of graveyards for the disposal of dead bodies, is not only a practice of human societies but is also observed in nature, including among small invertebrates such as termites. While the influence of termites on soil dynamics has largely been studied in comparing the specific properties of their mounds and protective she...

The symbiont-associated (SA) environmental package is a new extension to the minimum information about any (x) sequence (MIxS) standards, established by the Parasite Microbiome Project (PMP) consortium, in collaboration with the Genomics Standard Consortium. The SA was built upon the host-associated MIxS standard, but reflects the nestedness of sym...

Termites are key soil bioturbators in tropical ecosystems. Apart from mound nests constructed by some advanced lineages, most of the species use their faeces, oral secretions, debris, or soil aggregates to protect themselves from predators and desiccation when they go out to forage. Although this soil ‘sheeting’ is considered to play a key role in...

Termites primarily feed on lignocellulose or soil in association with specific gut microbes. The functioning of the termite gut microbiota is partly understood in a handful of wood-feeding pest species, but remains largely unknown in other taxa. We intend to feel this gap and provide a global understanding of the functional evolution of termite gut...

Wind erosion is a major threat to the sustainability of arid and semi-arid ecosystems. In these environments, biological soil crusts positively impact soil resistance to erosion. Less is known, however, on the impact of soil bioturbation by animals. In Southern Tunisia, bioturbation is mainly carried out by termites, ants and rodents which deposit...

Rafts of drifting pelagic Sargassum that are circulating across the Atlantic Ocean are complex ecosystems composed of a large number of associated species. Upon massive stranding, they lead to various socio-environmental issues including the inflow of contaminants and human health concerns. In this study, we used metabarcoding approaches to examine...

Knowledge of associations between fungal hosts and their bacterial associates has steadily grown in recent years as the number and diversity of examinations have increased, but current knowledge is predominantly limited to a small number of fungal taxa and bacterial partners. Here, we screened for potential bacterial associates in over 700 phylogen...

Spirochetes of the genus Treponema are surprisingly abundant in termite guts, where they play an important role in reductive acetogenesis. Although they occur in all termites investigated, their evolutionary origin is obscure. Here, we isolated the first representative of ‘termite gut treponemes’ from cockroaches, the closest relatives of termites....

The oxalate-carbonate pathway (OCP) is a biogeochemical process linking oxalate oxidation and carbonate precipitation. Currently, this pathway is described as a tripartite association involving oxalogenic plants, oxalogenic fungi, and oxalotrophic bacteria. While the OCP has recently received increasing interest given its potential for capturing ca...

Symbiotic digestion of lignocellulose in the hindgut of higher termites is mediated by a diverse assemblage of bacteria and archaea. During a large-scale metagenomic study, we reconstructed 15 metagenome-assembled genomes of Bathyarchaeia that represent two distinct lineages in subgroup 6 (formerly MCG-6) unique to termite guts. One lineage (TB2; C...

Nephridiophagids are unicellular eukaryotes that parasitize the Malpighian tubules of numerous insects. Their life cycle comprises multinucleate vegetative plasmodia that divide into oligonucleate and uninucleate cells, and sporogonial plasmodia that form uninucleate spores. Nephridiophagids are poor in morphological characteristics, and although t...

Symbiotic digestion of lignocellulose in the hindgut of higher termites is mediated by a diverse assemblage of bacteria and archaea. During a large-scale metagenomic study, we reconstructed 15 metagenome-assembled genomes (MAGs) of Bathyarchaeia that represent two distinct lineages in subgroup 6 (formerly MCG-6) unique to termite guts. One lineage...

Nephridiophagids are unicellular eukaryotes that parasitize the Malpighian tubules of numerous insects. Their life cycle comprises multinucleate vegetative plasmodia that divide into oligonucleate and uninucleate cells, and sporogonial plasmodia that form uninucleate spores. Nephridiophagids are poor in morphological characteristics, and although t...

Background Over the last decade, intensity and frequency of Sargassum blooms in the Caribbean Sea and central Atlantic Ocean have dramatically increased, causing growing ecological, social and economic concern throughout the entire Caribbean region. These golden-brown tides form an ecosystem that maintains life for a large number of associated spec...

Streets are constantly crossed by billions of vehicles and pedestrians. Their gutters which convey stormwater and contribute to waste management, and are important for human health and well‐being, probably play a number of ecological roles. Street surfaces may also represent an important part of city surface areas. To better characterize the ecolog...

Although mutualistic associations between animals and microbial symbionts are widespread in nature, the mechanisms that have promoted their evolutionary persistence remain poorly understood. A vertical mode of symbiont transmission (from parents to offspring) is thought to ensure partner fidelity and stabilisation, although the efficiency of vertic...

“Higher” termites have been able to colonize all tropical and subtropical regions because of their ability to digest lignocellulose with the aid of their prokaryotic gut microbiota. Over the last decade, numerous studies based on 16S rRNA gene amplicon libraries have largely described both the taxonomy and structure of the prokaryotic communities a...

The ability to digest lignocellulose with the help of their prokaryotic gut microbiota has enabled higher termites to colonize all tropical and subtropical regions. Over the last decade, numerous studies based on 16S rRNA gene amplicon libraries have largely described both the taxonomy and the structure of the prokaryotic communities associated wit...

Studies on termite symbiosis have revealed that significant symbiont lineages are maintained across generations. However, most studies have focused only on the worker caste. Little is known about the gut microbiota of reproductives, the most probable vectors for transmitting these lineages to offspring. Using 16S rRNA gene-based Illumina MiSeq sequ...

There are multiple forms of interactions between termites and bacteria. In addition to their gut microbiota, which has been intensively studied, termites host intracellular symbionts such as Wolbachia. These distinct symbioses have been so far approached independently and mostly in adult termites. We addressed the dynamics of Wolbachia and the micr...

Fungi and bacteria are found living together in a wide variety of environments. Their interactions are significant drivers of many ecosystem functions and are important for the health of plants and animals. A large number of fungal and bacterial families are engaged in complex interactions that lead to critical behavioural shifts of the microorgani...

Oxalate oxidation and carbonate precipitation were investigated associated to the oxalogenic tree Terminalia bellirica. Calcium oxalate crystals, oxalotrophic bacteria (dominated by genera Methylobacterium and Burkholderia), and carbonate accumulation (82% dry weight), were detected in the bark. In contrast, only a slight accumulation of carbonate...

In most cities, streets are designed for collecting and transporting dirt, litter, debris, storm water and other wastes as a municipal sanitation system. Microbial mats can develop on street surfaces and form microbial communities that have never been described. Here, we performed the first molecular inventory of the street gutter-associated eukary...

The complete mitochondrial genomes (mitogenomes) of two higher termites were reconstructed from the metagenomes of individual gut compartments with more than 2000-fold coverage. The circular mitogenomes of Labiotermes labralis (accession number KY436201) and Embiratermes neotenicus (accession number KY436202) have a length of 15,935 and 15,868 bp a...

Soils are complex ecosystems in which fungi and bacteria co-exist and interact. Fungal highways are a kind of interaction by which bacteria use fungal hyphae to disperse in soils. Despite the fact that fungal highways have been studied in laboratory models, the diversity of fungi and bacteria interacting in this way in soils is still unknown. Funga...

Bacteria and fungi naturally coexist in various environments including forest ecosystems. While the role of saprotrophic basidiomycetes in wood decomposition is well established, the influence of these fungi on the functional diversity of the wood-associated bacterial communities has received much less attention. Based on a microcosm experiment, we...

Oxalate is present in environments as diverse as soils or gastrointestinal tracts. This organic acid can be found as free acid or forming metal salts (e.g. calcium, magnesium). Oxalotrophy, the ability to use oxalate as carbon and energy sources, is mainly the result of bacterial catabolism, which can be either aerobic or anaerobic. Although some o...

Previous surveys of the gut microbiota of termites have been limited to the worker caste. Termite gut microbiota has been well documented over the last decades and consists mainly of lineages specific to the gut microbiome which are maintained across generations. Despite this intimate relationship, little is known of how symbionts are transmitted t...

We present software package for classifying protein or nucleotide sequences to user-specified sets of reference sequences. The software trains a model using a multiple sequence alignment and a phylogenetic tree, both supplied by the user. The latter is used to guide model construction and as a decision tree to speed up the classification process. T...

Wood recycling is key to forest biogeochemical cycles, largely driven by microorganisms such as white-rot fungi which naturally coexist with bacteria in the environment. We have tested whether and to what extent the diversity of the bacterial community associated with wood decay is determined by wood and/or by white-rot fungus Phanerochaete chrysos...

Diatoms, the major contributors of the global biogenic silica cycle in modern oceans, account for about 40% of global marine primary productivity. They are an important component of the biological pump in the ocean, and their assemblage can be used as useful climate proxies; it is therefore critical to better understand the changes induced by envir...

Influence of the external pH on valves morphometry. The eight morphometric traits measured on purified valves from T. weissflogii cells grown at the different pHs are: the average cell width and the number (N) of central fultoportulae, the minimum distance (l) between two adjacent central fultoportulae, the minimum distance (L) between two adjacent...

Dynamics of frustule formation in T. weissflogii. This real-time movie shows the evolution of the fluorescence of the dye used to follow valve formation in diatoms. The entire movie corresponds to 31 images acquired at 3.33 mHz, and the quantification of the HCK-123 is presented in Figure 4A. The left panel corresponds to the quantification and the...

Calibration curves used to measure the intracellular pH. (A) Background corrected 485/436 ratios of BCECF. The in vitro calibration corresponds to BCECF-free acid (5 µM) in a wide range of pH buffer values. Mean and SD correspond to three independent experiments and from 8 to 30 measures. (B) In situ calibration of BCECF-AM (5 µM) loaded cells afte...

Step by step image analyses for the extraction of several valve morphometric traits. (A) Original TEM image. The scale bar corresponds to 100 nm. (B) The same image after noise reduction. (C) Image after binarization. (D) Determination of the circular regions which correspond to the valve pores (in green), directly allowed us to determine the pore...

Boxplots showing the fluorescence intensity of cells labeled with different concentrations of HCK-123. T. weissflogii cells grown for 24 hours in the presence of HCK-123 were analyzed by flow cytometry, with a total of 3,800 to 5,800 analyzed cells. These results support the idea that HCK-123 is quantitative incorporated into the newly synthesized...

Influence of the environmental pH on valve morphology. We used TEM images at resolution level from the nanometer to the micron-scale to test the impact of the environmental pH on morphometric traits of the valve. (A) The width (W) of the valve (10≤n≤21). (B) The number (N) of central fultoportulae per cell (10≤n≤22). (C) The minimum distance betwee...

Scanning electron micrographs of the centric diatom Thalassiosira weissflogii. (A) View of the valve exterior showing the network on loculate areola. Note the presence of central fultoportulae, and of rimoportulae. (B) Semicontinuous cribra are present on the valve face. The interior opening of rimoportulae and the fultoportulae is visible. The sca...

Fluorescence properties of the Lysotracker HCK-123. Influence of the pH on the maximum emission of 1 µM HCK-123 in either 100 mM potassium hydrogen phosphate buffer or in 20 mM phosphate/citrate buffer. The intensity was normalized to the value obtained at pH = 7.0. (PDF)

Duration of the initial phases of valve formation. The duration of the two initial periods of valve formation was determined from cells grown at different pHs. (A) Length of the exponential phase (tExp). (B) Length of the decay phase (tDec). The data that correspond to 3 to 9 independent experiments were extracted from the recording of 8 to 92 indi...

Valves formation and separation of daughter cells. These movies which correspond to 97 images acquired at 3.33 mHz, reveal the process of cell separation. The left panel corresponds to the DIC images and the right panel to the images of the HCK-123 fluorescence. (AVI)

Calibration of the fluorescence intensity as a concentration of HCK-123. In situ determination of the fluorescence signal for 9 different concentrations (between 0 and 62.5 µM) of HCK-123. The method used was the same method as the one used to determine the signal inside T. weissflogii cells. The data that correspond to 3 independent experiments fr...

The supplemental methods contains: assessment of the use of HCK-123 as a reporter for valve formation, image analyses, morphometric analyses, modeling, and additional references. (DOCX)