Viktoriia Komarysta

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• PhD in Biology (Biochemistry)
• Professor (Associate) at V. N. Karazin Kharkiv National University

Haematococcus pluvialis Flotow is a unicellular alga that causes the red "bloom" of rain puddles. Most usually cultivated outdoors, H. pluvialis is used as a source of red pigment and antioxidant carotenoid astaxanthin for food and feed. We hypothesize that a limited number of factors exist which contribute the most to the culture growth and cellul...

Irradiation with a color temperature of 2700 K could be recommended for biomass growth in the industrial cultures of H. lacustris and D. salina. After the biomass is grown, 6400 K light should be applied along with carotenoid accumulation conditions (or 2700 K retained for D. salina). The neutral light possesses advantages neither for culture growt...

The algal pyrenoid is a naked phase-separated liquid compartment inside the chloroplast consisting predominantly of densely packaged Rubisco and most often transversed by a system of lipid membranes. The pyrenoid participates in carbon-concentrating mechanisms of algae. During the cell division, the daughter cells of algae acquire the pyrenoids via...

The algal pyrenoid contains intrinsically disordered DNA-binding proteins which could be capable of liquid-liquid phase separation and provide DNA co-localization with the liquid phase separated pyrenoid organelle.

The pyrenoid is a compartment containing Rubisco in the chloroplasts of some algae and hornworts absent from the terrestrial plants. Recently, using DAPI-staining and laser confocal microscopy, we observed co-localization of the pyrenoid and chloroplast nucleoid and their simultaneous even fission via a dumbbell structure during the chloroplast div...

The research aimed to compare climatic conditions at the production sites in Australia and the potential locations for the renewal of D. salina production in Ukraine and discuss the influence of the climate on the development of the alga.

This work is aimed at quantification of cell yield, total lipid and carotenoid contents in the cells of three algal species, carotenogenic D. salina and H. pluvialis, and non-carotenogenic Dunaliella viridis Teodoresco, under different salinity and irradiance, nitrate and phosphate starvation or supplementation, and bicarbonate addition.

This study is an attempt to meta-analyze data on the transcriptome of D. salina under varying salinity conditions. The objective of the study was to establish which genes undergo the highest expression change when salinity increases or decreases and what their expression profiles are. For the meta-analysis, a dataset PRJNA295823 from the NCBI SRA d...

Проведений нами мета-аналіз додає деякі нові результати до раніше опублікованого за набором даних PRJNA295823. Висока солоність швидко активувала масивну експресію генів біосинтезу гліцерину (DAR1), мітохондріального дихального ланцюга (NUOB12, cox1), метаболізму вуглеводів - фіксації вуглецю (rbcS) і біосинтезу кофактора тіаміну, а також генів цит...

The intensity of nitrate and phosphate absorption in the fed-batch culture of D. salina increases (within the cell quota) with an improvement in the gas exchange due to air bubbling of the culture and a decrease of the cell suspension layer thickness, as well as with the replacement of the culture fluid with a fresh nutrient medium, that also might...

The objective of this research is to study how the deficiency of nitrate affects the dynamics of phosphate acquisition and vice versa, in cultures of H. pluvialis, Ch. dissectum and D. salina at two levels of light intensity (2 and 8 klx) and two levels of salinity (1 and 4 M NaCl) for saltwater D. salina. In both freshwater species H. pluvialis an...

The alga species investigated might be regarded as the potential source of carotenoids, possibly astaxanthin. The alga possesses a relatively high culture growth rate. Further research is necessary on exact species taxonomic attribution, factors of carotenoid accumulation induction, cell carotenoid content HPLC confirmation, and effective methods o...

Beta-ionone is a carotenoid derivative often found in plant essential oils. It can also be synthesized chemically. In H. pluvialis beta-ionone stimulated carotenoid accumulation more than twice under nitrogen starvation and high light. The observed effect of beta-ionone suggests that apocarotenoids could play the signal role in carotenogenesis indu...

Green microalgae Dunaliella salina Teodor. and Haematococcus pluvialis Flotow accumulate large quantities of secondary carotenoids in their cells under certain conditions, induce red “bloom” of certain natural habitats, and are cultured to manufacture these valuable ingredients for use in food, feed, and cosmetics. Both species have in common that...

Culture growth and β-carotene accumulation in green microalga Dunaliella salina, one of the sources of β-carotene in the industrial biotechnology, are controlled by several parameters and their interactions: nitrate and phosphate supply and nutrient reserves in the cells, salinity, and irradiance. It is difficult to infer about the mechanisms of in...

The purpose of the study was to evaluate the contribution of salinity, irradiance, nitrate and phosphate, and their interactions into the yield of cell number and beta-carotene accumulation in Dunaliella salina. To avoid confounding of the effects of factors-conditions by the depletion of factors-resources, the alga was grown in fed-batch culture....

The present research attempts to calibrate satellite images against field data on D. salina ß-carotene concentration expressed per 1 L of brine. We found the empirical relation between ß-carotene quantity and the index based on two infrared reflectance bands (NIR and SWIR).

There were studied D. salina culture growth, beta-carotene and total protein accumulation under various fed-batch regimes: supplementation of nitrate and phosphate, nitrate or phosphate solely, and after the switch of nitrate supplied culture to phosphate supplementation and vice versa. Pre-cultures, unsupplied with the both or any nutrient, accumu...

At alternating cultivation cycles under deficiencies of nitrogen or phosphorus D. salina culture grew limitedly at the first cultivation, then intense after transferring the culture. Cellular beta-carotene accumulated during the first cultivation, and then almost did not. D. salina cells are able to accumulate the available nutrient at the other nu...

The paper presents the results of expedition research of some solar salt works of the South of Ukraine (Kherson region, AR Crimea) and lake Baskunchak (Astrachan region, Russia), as well as stationary observations on populations of the microalga Dunaliella salina Teod. in the ponds of Heroyske salt works (Gola Prystan’ district, Kherson region) tha...

Deficiency of nitrogen and phosphorus induced beta-carotene accumulation in the cells of 35 clonal cultures of D. salina isolated from 6 habitats in Ukraine and 1 in Russia. Nitrate and phosphate re-supplied into the medium decreased cellular beta-carotene content and cell size. Repeated passage into the medium without nutrients recovered inducible...

Установлено, что помимо накопления -каротина, на дефи- цит азота или фосфора клетки по-разному реагировали изменением других компонентов системы антиоксидантной защиты: дефицит азота приводил к снижению активности каталазы и содержания бел- ка в клетках; дефицит фосфора, напротив, вел к накоплению белка в клетках; активность каталазы при дефиците...

Catalase activity in the cells of Dunaliella salina cultures grown under conditions inducing carotenogenesis, such were the lack of nitrogen and phosphorus, and different supply with this biogens was estimated. The nitrogen lack and the lack of both biogens led to the catalase activity decrease per cell and per protein. The catalase activity remain...

Size, shape and biochemical characteristics variability of Dunaliella salina cells depending on nitrogen and phosphorus culture supply was revealed. The possibility of these signs use for indication of biogenes content in natural ponds is considered.

The effect of suboptimal concentration and the exclusion of nitrogen and phosphorus from the medium on β-carotene accumulation by Dunaliella salina Teod. cells, culture growth dynamics, and the uptake of these nutrients under favorable conditions for cell division values of salinity, illumination, and temperature were investigated. Phosphorus exclu...

Дефицит азота является наиболее мощным индуктором накопления b-каротина в клетках микроводоросли Dunaliella salina Teodoresco, и то же время ингибирует рост культуры. Среда обитания D. salina-природные рассолы, характеризующиеся низкой концентрацией растворенных газов, и углекислый газ также может быть лимитирующим фактором для роста культуры и кар...

In this paper, there are presented the research results on the influence of the nutritional medium density and KNO3 addition on the culture growth and cellular beta-carotene content in D. salina cells cultivated for 21 days in the Artari medium and the medium prepared from the sea salt under the natural illumination at the direct sunlight.

Цель работы – установить, может ли обогащение рапы данными источниками углерода способствовать красному цветению D. salina. Концентрация бикарбоната натрия 10 г/л оказалась ингибирующей для культуры, возможно, за счет резкого сдвига pH в щелочную область. Концентрации 10 и 100 мг/л увеличивали выход клеток в стационарной фазе роста в 1,4 и 1,7 раза...

Микроводоросль Dunaliella salina Teod. вызывает красное цветение бассейнов солепромыслов и самосадочных соленых озер. D. salina является богатейшим природным источником бета-каротина и перспективным объектом биотехнологии. Множество работ посвящено поиску факторов и подбору условий, которые позволили бы получать высокие урожаи биомассы и бета-карот...

В данной работе представлены результаты наблюдений за образованием и прорастанием зигот в висячей капле на среде Артари при естественном освещении (западное окно).

Сохранение клеточных оболочек в препаратах после проведения ацетолиза является доказательством присутствия в них спорополленина. Тест на спорополленин проводили в препаратах вегетативных клеток и цист Haematococcus pluvialis Flot. emend. Wille, накапливающего внепластидный каротиноид астаксантин и его жирнокислотные эфиры. Микроскопия препаратов по...

Zygotes were produced in a laboratory culture of Dunaliella salina Teod. (Chlorophyta). The presence of sporopollenin, a biopolymer resistant to acetolysis, was established in the composition of their cell walls. The function of β-carotene, accumulating in the lipid-carotenoid globules in cells of these microalgae, is discussed as a possible precur...

Total fatty acids of D. bardawil ATCC 30861 cells were analyzed with the gas chromatography method.

The content of carotenoids and neutral lipids in cells of two species of microalgae of the genus Dunaliella Teod. is influenced the process of their cultivation from the 4th to the 24th day of growth and also in the initial concentration of cells, lighting, and temperature on carotenogenesis and lipogenesis have been investigated. It is shown (hat...