How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
Myself Dr. Vijayakumar working as Research Associate at Molecular Biophysics, IISc. I completed Ph.D in Biochemistry from North-Eastern Hill University, Shillong. During Ph.D, I worked on structural and functional characterizations of asparaginyl tRNA synthetase from liver fluke. I have published more than 20 publications in national and international journals. Also life member in Society of Biological Chemists (India), Indian Biophysical Society, International Society of Infectious Diseases.
I did Tryptophan quenching studies of my truncated protein excitted at 295nm with the emmission range 300-500 nm. I am getting two peaks one at 335nm (one for tryptophan) and other at 380nm. The later peak was more intense than the earlier one. This patter was same in all the quenching conditions including blank (only protein with buffer). Whereas the full length protein emits only at 335nm. I checked the same condition with buffer alone too , but there is no any peak at 380nm. Can anyone explain why this happens?
Thanks in advance.