Vijay Kumar Singhal

Vijay Kumar Singhal
Punjabi University, Patiala · Department of Botany

Ph.D

About

174
Publications
32,443
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1,948
Citations
Citations since 2017
56 Research Items
862 Citations
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Introduction
I am engaged in research pertaining to cytogenetics and systematics of Angiosperms. I have published more than 194 research papers on taxonomy, forest vegetation and cytomorphological diversity. I have guided 14 Ph.D. and 10 M.Phil. students mainly on the cyto-morpholgical diversity, germplasm evaluation and reproductive biology.
Additional affiliations
January 2014 - present
Punjabi University, Patiala
Position
  • Professor (Full)
January 1983 - January 2013
Punjabi University, Patiala

Publications

Publications (174)
Article
Present communication records polymorphic and polyploid nature of the forest grass species M. effusum having wide distribution over different continents and countries. Globally the 4 x plants are widely distributed. The West Himalayan plants, collected at 3000 m alt. and more, are also sporting chromosome number of 2n = 28 based on basic number of...
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Disparity or continuity in the chromosome count within taxa of various categories has been verified to be imperative character for taxonomic classification. Male meiotic studies in 64 species (161 accessions) of composits from geographically isolated regions were examined in detail in order to update the existing knowledge of meiotic chromosome num...
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The nature of plant medicines is decided by the secondary metabolites it contains which may fluctuate with the altitude. We here studied the variation in total phenols and flavonoids and three marker compounds viz., atropine, caffeic acid, and chlorogenic acid in different plant parts of Hyoscyamus pusillus collected from three sites having varying...
Article
Physochlaina praealta samples were studied macromorphologically and cytomorphologically along with their detailed phytochemical investigation. The concentration of phytoconstituents showed a strong positive correlation with the ploidy level and altitudinal gradients. The total phenol content was detected maximum in the methanolic extract of leaves...
Article
Two cytotypes of ‘giant fescue’ sporting gametic chromosome number of n = 14 and n = 21 corresponding to 4x and 6x ploidy level (based on x = 7) were encountered in the various localities in India falling in the Western Himalayan region. The pollen mother cells of hexaploid cytotype also exhibited presence of B chromosomes (n = 21 + 0 − 2B). The in...
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The present communication reports the record of intraspecific polyploids (4x, 12x) in Potentilla atrosanguinea from the cold deserts of Ladakh, India. The individuals of the specie showed morphogenetic variations in the studied area and two variants have been recoginized on the basis of flower colour and chromosome count. The study included the mal...
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Present cytological study records existing chromosome number diversity, their male meiotic course and pollen fertility analysis in the two wheatgrass species of tribe Triticeae Dumort. (Poaceae) from Western Himalaya, India. Agropyron thomsonii Hook. f. is an endemic grass of alpine zones of Western Himalaya and Elymus nutans Griseb., a widely dist...
Chapter
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The Lactuca genus, which is characterized by a variety of forms including annual, biennial and perennial, glabrous or pubescent herbs with abundant latex, belongs to the Asteraceae family. Overall taxonomic literature has shown that 100-111 species represent the genus. The members are found primarily in warm and temperate regions mostly in the Nort...
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The present communication reports the existence of intraspecific euploid cytotypes (2n = 28, 56) and secondary chromosomal associations in Geranium pratense L. from the cold deserts of Ladakh (India). The study includes the detailed male meiosis, microsporogenesis, pollen fertility and morphometric analysis in the accessions of 2x and 4x cytotypes....
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The intraspecific euploid (2x, 4x) cytotypes have been reported in Tragopogon pratensis from the cold deserts of Ladakh division in Jammu and Kashmir. Both the cytotypes showed perfect bivalent formation, regular meiotic course and normal microsporogenesis leading to high pollen fertility. The 4x plants are more vigorous and growing much taller tha...
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The present communication reports the meiotic analysis on three wild populations of Rorippa palustris collected from Ladakh division, Jammu & Kashmir and records the existence of first ever intraspecific euploid cytotypes, the diploid (2n=14) and hexaploid (2n=42). Beside chromosome number, the two cytotypes also differ in morphometric parameters s...
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Meiotic studies have been carried out on three plants of Leontopodium jacotianum from Cold deserts of Ladakh. The chromosome number of n=24 counted in all the plants adds a new tetraploid cytotype for the species. The plants collected from Zoji-La showed a normal meiotic course leading to high pollen fertility. However, the plants collected from Sa...
Article
Available at: https://www.jstage.jst.go.jp/article/scr/21/2-4/21_61/_pdf/-char/ja Till date, several classical and modern methodological tools had been employed to investigate the phenomenon of cytomixis for developing a clear understanding about its occurrence, causes, mechanism, and role in plant evolution. These attempts have succeeded to some e...
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Meiotic studies have been carried out on six accessions of Artemisia roxburghiana from four regions of Western Himalayas. The accessions collected from Chamba, Kinnaur and Kullu districts of Himachal Pradesh and Kargil district of Jammu and Kashmir showed a normal meiotic course leading to high pollen fertility. However, the accession collected fro...
Article
Present study on male meiotic analysis of wild grass species from Uttarkashi district hills, Uttarakhand, India provides chromosome numbers for 10 species belonging to tribe Aveneae, family Poaceae. The study reports chromosome numbers firstly for Helictotrichon uniyalii (2n=4x=28, Rarhi Valley), Trisetum aeneum (2n=2x=14, Assi Ganga Valley) and Tr...
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Meiotic studies carried out in Brassica rapa L. (Brassicaceae), which exists at diploid level (2n=20) revealed the presence of 0-3 B chromosomes in the collections from Ladakh division of Jammu & Kashmir in India. The chromosome number of n=10 from northwest Himalayas agrees with most previous reports. The presence of B chromosomes is the first rec...
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The Present study includes cytomorphological analysis on five species of Lactuca L. (Asteraceae) from the hilly district of Kinnaur, Himachal Pradesh (India). Lactuca orientalis (Boiss.) Boiss. (2n=18) which has been counted chromosomally for the first time from India also adds a new intraspecific 2x cytotype to the earlier tetraploid chromosomal r...
Article
Meiotic analysis on 26 wild plants of Phacelurus speciosus collected from Kullu district, Himachal Pradesh recorded the existence of three intraspecific euploid cytotypes, the diploid (2n=20), tetraploid (2n=40) and hexaploid (2n=60). Besides chromosome number, cytotypes also differ in morphometric parameters such as plant height, number of interno...
Article
Traditional knowledge has been used throughout the world including India as a practice of using local herbal drugs. Majority of the locals living in the district Uttarkashi of Uttarakhand state have poor connectivity and socio-economic status. These people are known to have indigenous experience about the medicinal plants of the area and use their...
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Background Meiotic abnormalities lead to morphological and genetic variations which caused not only to evolution but also intraspecific reproductive barriers. During present study of detailed meiotic course in dicotyledonous plants sampled from Indian cold deserts, various meiotic abnormalities have been detected. For this, the plant materials fixe...
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Artemisia nilagirica (Asteraceae), an important medicinal herb, is widely distributed in the hilly regions of India. On accession based analysis from Western Himalayas in Kullu district, Himachal Pradesh, we here report the presence of individuals with different chromosome counts (2n = 36, 54) and two ploidy levels (2n = 4x, 6x). Both the cytotypes...
Article
Present study explores the meiotic chromosome numbers in seven species of the genus Elymus from Himachal Pradesh and Uttarakhand, India. The study adds the first chromosome number of E. gangotrianus (n=14+0-2B), besides adding chromosome numbers for Indian taxa of E. himalayanus (n=21), E. jacquemontii (n=14), E. schrenkianus (n=21) and E. schugnan...
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Male meiotic studies were carried out on eight different accessions of Hedysarum astragaloides Benth. ex Baker (Fabaceae), an endemic and threatened species of northwest Himalaya, India. Although genetic factors such as meiosis, chromosome number, and ploidy level may be causative for the evolution, endemism, rare distribution or even extinction of...
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Chromosome counts including analyses of male meiosis and pollen fertility have been made on 36 accessions of 10 Lactuca species collected from the western Himalayas. L. orientalis (2n=18) and L. undulata (2n=18) have been counted chromosomally for the first time from India and the counts in other species confirmed previous counts. The study also ad...
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Intraspecific euploid cytotypes (2n=18, 36) were found in the wild plants of Brachyactis pubescens in North West Himalayas from the hills of Ladakh division of Jammu and Kashmir and Solang Valley, Kullu district, Himachal Pradesh. The plants with each ploidy level showed regular bivalent formation, equal segregation of chromosomes during anaphases...
Article
The present study covers chromosome counts, male meiosis and pollen fertility in 164 species of dicots from the Parvati Valley, Kullu district, Himachal Pradesh. Gathered cytological information generates first ever chromosome counts, new diploid/polyploid cytotypes and varied chromosome counts. As many as 41 species (25%) showed intraspecific eupl...
Article
The present study includes chromosome count, meiotic abnormalities, pollen fertility and karyotype of Elymus semicostatus from north-west Himalaya, India. This is the first attempt to present karyotype morphometric data of the species from India [2n = 4x = 28 = 22m(2sat) + 6sm]. At present 28 wild accessions of the species have been analysed, all o...
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Meiotic investigations carried out from the cold desert regions of Ladakh (India) record the first ever chromosome count (2n = 32) and cytomixis in Clematis ladakhiana. The phenomenon of cytomixis involving chromatin transfer among 2–6 adjacent pollen mother cells (PMCs) through cytoplasmic channels was observed at different meiotic stages. The chr...
Article
Male meiotic studies carried out on the plants of Fagopyrum acutatum (Polygonaceae) collected from the Parvati valley, Kullu district, Himachal Pradesh, India confirms the earlier chromosome reports of 2n=16 from outside of India. The presently recorded chromosome count of n=8 in pollen mother cells (PMCs) is the first ever report of a diploid cyto...
Article
Male meiotic studies have been performed on three wild accessions of Arthraxon hispidus scored from Parvati valley and Solang valley of Kullu district in Himachal Pradesh. The accessions shared the same diploid chromosome number of 2n=30 and added a variable dysploid chromosome report for the species against the earlier reports of 2n=18, 36, 38, 40...
Article
The current study report the chromosome counts for twelve species of tribe Epilobieae from the cold desert regions of north-west Himalaya. These chromosome counts add to our existing database and enlighten our understanding of the cytology and distribution pattern of the tribe. First chromosome counts for two species, Chamerion speciosum (Decne.) H...
Article
Male meiotic studies have been performed on plants basis in Deutzia staminea collected from three localities of Parvati valley, Kullu district, Himachal Pradesh, India. The scored accessions uniformly exhibited the diploid chromosome count of 2n=26 confirming the earlier reports from other regions of North-West Himalayas. The individuals studied fr...
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The present communication reports the first record of intraspecific euploid cytotypes (2x, 4x) in Arabis recta from the cold deserts of North-West Himalayas in India. The study included the detailed male meiosis, microsporogenesis, pollen fertility and morphometric parameters in the accessions of 2x and 4x cytotypes. Both the diploid and tetraploid...
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Angelica glauca Edgew. (Apiaceae) has been cytologically studied from the cold deserts of Ladakh division of North-West Himalayas in India. Besides re-reporting the diploid chromosome count of 2n=22 from India, the analyzed accession depicted the phenomenon of cytomixis, cell fusion leading to syncyte PMCs and dimorphic pollen grains. Majority of t...
Article
Exploratory surveys were carried out in the high altitude areas of the northwest Indian Himalaya to analyze and appraise the cytomorphological diversity among the wild flowering plants. During the random exploratory surveys, we found morphological variations in some species viz., Dianthus angulatus Royle ex Benth. (Caryophyllaceae), Geranium wallic...
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Summary The existence of intraspecific euploidy (2n=22, 44) in Primula denticulata have been reported in the present study from the higher hills of North-West Himalayas in India. Both the cytotypes showed perfect bivalent formation, regular meiotic course and high pollen fertility. One of the 4x accessions showed a phenomenon of cytomixis and cell...
Article
Accession based analysis in Apluda mutica studied from cytologically unexplored and phytogeographically isolated region of Uttarakhand, India revealed the existence of diploid chromosome count of 2n=20, normal meiosis and high pollen fertility. Individuals of three wild accessions however showed the presence of one to two B-chromosomes in 25.64 to...
Article
The accession showed the tetraploid chromosome number of 2n=28 and irregular meiotic course due to the presence of multivalents, univalents, scattered and unequal distribution of chromosomes, chromatin bridges and laggards. Besides, a few PMCs showed cytomixis and cell fusion resulting into syncytes. Meiotic course in syncytes was also irregular. C...
Article
Objective:The objective of the present study was to enlist the ethnobotanical uses and chromosomal status of wild plants of Parvati Valley, Himachal Pradesh. Plants are locally used in curing ulcers, sores, insect bites, muscular and joint pains, pneumonia, jaundice, diarrhoea, piles, swellings, allergies and skin diseases. Methods:For documentatio...
Article
Present study records the existence of tetraploid and hexaploid meiocytes and subsequently the formation of large and very large-sized pollen grains for the first time in the diploid individuals of Achillea millefolium. In majority of the diploid accessions, meiocytes exhibited nine bivalents, equal segregation of chromosomes during anaphases, regu...
Article
In current paper, we studied the meiotic chromosome number and details of cytomixis recorded for the first time in Thalictrum cultratum Wall. from the high altitude areas of northwest Himalaya (India). Cytomixis, the phenomenon of inter PMC (pollen mother cell) migration of chromatin material has been reported in many angiosperms and other groups o...
Article
Impatiens (Balsaminaceae), a genus of over 1000 species, is represented by 209 species in India, particularly in South India and Himalayas. In spite of attempts made by several Indian cytologists, the chromosome counts are available for only 95 species, through karyological studies, or pollen mitosis or meiotic preparations. The investigated specie...
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Objective: The objective of the study was to enlist the ethnobotanical uses of wild plants of Solang Valley, Kullu District, Himachal Pardesh. Due to the specific geographical location of the valley, it possesses a high amount of endemic plant diversity. The plants are being used medically by local people against many ailments such as rheumatism, g...
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Male meiotic studies were performed on 14 wild accessions of Impatiens devendrae Pusalkar from Western Himalayas. The species which is endemic to the state of Uttarakhand has been worked out for chromosome counts for the first time. Aim of the study was to analyse in detail the male meiotic course, microsporogenesis and pollen formation. All the sc...
Article
On the basis of meiotic studies carried out presently on Inula grandiflora from Malana Valley in Kullu district, Himachal Pradesh, we here report the existence of cytomixis and intraspecific diploid (n=8) and tetraploid (n=16) cytotypes. The 4x individuals grow much taller and possess larger sized leaves and capitula. Stomata and pollen grain chara...
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Physochlaina praealta (family: Solanaceae), a widely distributed species of Indian cold deserts, is studied for male meiotic studies. We here report for the first time the existence of intraspecific polyploidy (2x, 4x) in the species. Both the 2x and 4x individuals show perfectly normal meiotic behavior and nearly 100% fertile pollen grains. The 4x...
Article
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In current investigation we studied the male meiosis and details of secondary chromosomal associations or pairing recorded for the first time in Dianthus angulatus Royle ex Benth., from the cold deserts of Lahaul-Spiti (Himachal Pradesh, India). All the presently studied individuals of the species existed at 2x level (x = 15). The present chromosom...
Article
Chromosome counts including male meiotic studies have been made on 45 species (125 accessions) of Asteraceae collected from the Parvati Valley, Kullu district, Himachal Pradesh. Crepis foetida (n = 5) and Senecio pedunculatus (n = 20) have been counted chromosomally for the first time at global level. The genus Phagnalon has been worked out for the...
Article
An accession-based study carried out on Anthoxanthum odoratum from Kullu district revealed a tetraploid chromosome count of n=10 and the presence of multiple chromosomal associations of up to 10 chromosomes due to structural heterozygosity for reciprocal translocations. Owing to the presence of multivalents, the individuals showed considerable amou...
Article
[http://www.tandfonline.com/eprint/uACgWPsjE4aVj57HY5bv/full]:-------- Karyotype morphometric data of two species of the genus Clematis L., a cosmopolitan and diverse genus, were studied. Clematis flammula is a diploid with 2n = 16 chromosomes. It exhibits a gradual decrease in size of chromosomes from 14.83 (±3.6) µm to 7.58 (±3.65) µm. Its karyot...
Article
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Heracleum pinnatum C. B. Clarke (Apiaceae) collected from the cold deserts of Ladakh Himalaya, Jammu and Kashmir, India, is cytologically analyzed for the first time, revealing a diploid chromosome count of 2n=22. During meiosis, the majority of the pollen mother cells (PMCs) exhibited 11 bivalents, equal segregation of chromosomes during anaphases...
Article
Meiotic studies carried out in the individuals of Sedum oreades (Decne.) R. Hamet (Crassulaceae), which exists at diploid level (n=11) revealed the presence of 0-4B-chromosomes, non-synchronous disjunction of bivalents, chromatin bridges, and laggards as well as some pollen sterility (8%) in the collection from Manimahesh Hills (Gauri Kund, 3930 m,...
Article
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Male meiotic studies have been carried out on the wild plants of Tanacetum artemisioides Sch. Bip. ex Hook. f. collected from the cold desert regions of Ladakh Division of Jammu and Kashmir. The studied individuals, which existed at the 2x level (2n=18), showed structural heterozygosity for reciprocal translocations as indicated by the presence of...
Article
Nucleolus was regarded as a mere ribosome-producing factory for many decades however, in the past two decades; this has come to the light that it is a plurifunctional nuclear domain which plays vital role in many cellular processes. Despite many advances, much remains to be revealed about the nucleolus association with cellular processes. While exp...
Article
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In the present work, detailed male meiotic studies were performed on 24 dicot species from the hills around Shimla within the altitudinal range of 1880–3140 m and which include the first ever chromosome count for Polygonum polystachyum (n=11) from India. Further, a new intraspecific aneuploid cytotype of 2n=18 for Impatiens brachycentra and B-chrom...
Article
In the present investigation, we examined the morphological and ecological adaptations, and male meiosis and pollen fertility in an endemic species of genus Astragalus, A. rhizanthus Royle ex Benth. from cold deserts of Lahaul–Spiti district of Himachal, Pradesh, India. The three populations from Lahaul Valley and one from Spiti Valley depicted the...
Article
Pleurospermum Hoffm. (Apiaceae), a widely spread, heterogeneous genus of complex and controversial taxonomy, is poorly known for chromosome number and meiotic details from the Indian subcontinent. In the current study, we examined male meiosis, chromosome counts, and pollen fertility in two species, P. candollii and P. govanianum from alpine areas...
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Detailed meiotic investigations were performed in three accessions of Meconopsis aculeata scored from high altitudinal regions in the Northwest Himalayas. These accessions exhibit the same chromosome number of n=28 and exist at tetraploid level. We report here the occurrence of chromatin stickiness and abnormal spindle activity in the presently stu...
Article
Tussilago farfara of family Asteraceae has been worked out cytologically for the first time from India. The presently studied individuals collected from Solang Valley, Kullu District, Himachal Pradesh exist at triploid level with 2n=24 against the earlier highly polyploidy counts of 2n=60 and 2n=72 from outside of India. These individuals depict ab...
Article
The current investigation includes detailed male meiotic studies, chromosomal counts, and pollen fertility on 38 species of family Asteraceae from the Pangi Valley, a part of cold desert Himalayas. The first-ever chromosome counts have been made for seven species viz. Aster albescens Wall. (n = 9), A. heterochaeta C. B. Clark (n = 9), Cirsium invol...
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Meiotic studies have been carried out on 12 accessions of Anemone rivularis from different regions of Western Himalaya. The accessions from Chamba, Kullu and Kinnaur districts of Himachal Pradesh and Uttarkashi district of Uttarakhand showed a normal meiotic course leading to high pollen fertility (87–100%). However, the accession scored from Saat...
Article
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Detailed male meiotic studies were made in Lindelofia longiflora (Royle ex Benth.) Baill. var. falconeri (Cl.) Brand (Family: Boraginaceae) from the cold deserts of Pangi Valley, Chamba District (Himachal Pradesh) in India. The meiotic course in some of the individuals was observed to be highly abnormal which include cytomixis involving chromatin t...
Article
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Presently, A. millefolium, a morphogenetically variable species, has been scored from phytogeographically isolated and unexplored areas of the northwest Himalayas for chromosome counts and male meiotic course. All the studied accessions shared the same meiotic chromosome number of n = 9 and existed at diploid level. A majority of the accessions exh...
Article
The present work includes detailed male meiotic studies on 46 species of grasses falling into 59 accessions from different localities of Parvati Valley in Kullu district of Himachal Pradesh in the altitudinal range of 1,100 to 2,750 m. All the species have been studied cytologically for the first time from the study area. The meiotic chromosome cou...
Article
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In the present study, we report the phenomenon of cytomixis for the first time in some individuals of Nepeta govaniana showing chromatin transfer at the different stages of meiosis I. In most of the cases, two to six PMCs are involved in chromatin transfer. The migration of chromatin is either complete or partial, resulting in denucleated, double n...
Article
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Pangi Valley is the interior most tribal area in Himachal Pradesh of Northwest Himalaya. An ethnobotanical investigation is attempted to highlight the traditional knowledge of medicinal plants being used by the tribes of Pangi Valley. Various localities visited in the valley 2-3 times in a year and ethnobotanical information was collected through i...
Article
Click or copy paste link to view pdf for free on springerlink : http://rdcu.be/mIpE Despite the presence of intraspecific polyploidy (2x, 4x, 5x and 6x) in Agrimonia eupatoria, origin of these cytotypes has never been addressed adequately. The aim of the present study was to record the original chromosome counts and characterize chromosomal pairin...
Article
Tordyliopsis brunonis (Apiaceae) is cytologically investigated here for the first time from India. The chromosome count of 2n = 33, ascertained here, represents a new intraspecific triploid cytotype in the species, supplementing the earlier report of a diploid cytotype with 2n = 22 from Nepal Himalayas. The diploid chromosome count (n = 11) has als...
Article
Silene vulgaris is an important ethnobotanical species, but so far no thorough meiotic analysis has been performed, despite its wide occurrence in temperate and alpine Himalayas, this prompted us to undertake present study. We investigated original meiotic chromosome number and effect of cytomixis on meiotic course, microsporogenesis and pollen gra...
Article
Full-text available
Despite the presence of intraspecific polyploidy (2x, 4x, 5x and 6x) in Agrimonia eupatoria, origin of these cytotypes has never been addressed adequately. The aim of the present study was to record the original chromosome counts and characterize chromosomal pairing during meiosis and microsporogenesis in the 5x cytotype, and discussing the hypothe...
Article
Chromosome counts, detailed meiotic course, microsporogenesis, and pollen fertility studies have been carried out in two species of Trigonella, T. emodi Benth. and T. pubescens Edgew. ex Baker collected from the Kinnaur and Chamba districts of Himachal Pradesh (India). Both of the species from Kinnaur have been studied cytologically for the first t...
Article
Two varieties of Saxifraga diversifolia, diversifolia and parnassifolia, collected from the moist alpine slopes around Gauri Kund, 3930 m (Manimahesh Hills, Himachal Pradesh, India), are studied for detailed male meiosis, chiasma frequency and pollen fertility. Both varieties exist at diploid level (based on x = 8), and show a meiotic chromosome co...