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Directed evolution is a powerful approach to study the molecular basis of protein evolution and to engineer proteins for a wide range of applications in synthetic organic chemistry and biotechnology. There are many methods based on random or focused mutagenesis to engineer successfully any protein trait. Focused approaches such as site-directed and...
Assuming using an optimized secretion tag, is it worth to investigate an ELP-intein system to produce protein directly in the cultured media ?
I want to completely automate my Golden Gate cloning with a simple liquid-handling robot. I am using destination plasmids with negative marker (lacZ or RFP) and I want to know if someone already tried to use limiting dilution instead of plating after the transformation in order to get single colonies.
I am looking for the best strategy to dose albumine in human serum with SD = 0.1 % using 2-300 ul. No matter the cost.
Thanks for your help
I am starting with Ecoflex. Their is choice of RFP ou LacZ as negative markets. What do I need to chose for Level 1 and Level 2 reaction ?
PS: Does something add a new BpiI TypeIIs site for cloning Level0 plasmid with a golden gate reaction instead of classical cloning with BsaI ?
I am currently looking to know if it's possible to do a feeding experiment with some purified squalene on in vivo culture of E.coli that express some triterpenes cyclases.
I want to know if the squalene can go in/throw the plasmic membrane.
Thank you !
Nowadays the use whole cell or enzymes in modern chemical manufacturing processes is gaining ground over chemical synthetic processes. Traditional chemical methods often require time-consuming multistep-processes, harsh operating conditions and the use of chemical reagents and organic solvents that are expensive and environmentally harmful. Conversely, enzymatic production by whole cells or enzymes improves regio-, stereo- and enantioselectivity and provides an eco-friendly framework. As a result, the replacement of chemical strategies by enzymatic or chemo-enzymatic approaches is a common practice in pharmaceutical, textile, food, cosmetic and biomedical industries. In this respect, this Special Issue aims to gather the recent advances in the field of biocatalysis and biotransformations. https://www.sciencedirect.com/journal/biochimica-et-biophysica-acta-bba-proteins-and-proteomics/special-issue/10XXWKCMZF5 Topics include but are not limited to the following - Directed evolution - Protein engineering - Bioprocesses catalyzed by proteins or whole cells - Enzyme immobilization - Bioprocess Engineering - Enzymatic and/or chemoenzymatic synthesis of high value-added compounds - Applied Biotechnology - Environmental Biocatalysis - Agro- and Food Biotechnology - Industrial Biotechnology