About
3
Publications
928
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
19
Citations
Introduction
I am a Microbiologist, with a wide knowledge of marine microbiology, describing microbiomes from various sources (salmon gills, water, sediments). I have experience in bacterial pathogenicity, genomic analyses, bioinformatics, and molecular biology.
Skills and Expertise
Additional affiliations
March 2022 - December 2022
Position
- Research Assistant
Description
- I worked at the UniCoV project, detecting SARS-CoV-2 in saliva samples during the COVID-19 pandemic, by using real-time PCR.
Education
April 2017 - May 2022
September 2014 - July 2016
September 2009 - July 2014
Publications
Publications (3)
Amoebic gill disease (AGD) and complex gill disease (CGD) are recurrent gill disorders in Atlantic salmon, resulting in significant aquaculture losses. The role of gill microbiomes in gill disease development is unclear. We undertook a longitudinal study to characterise the gill tissue and gill mucus microbiomes of farmed Atlantic salmon before, an...
Background Amongst gill disorders in Atlantic salmon, amoebic gill disease (AGD) is currently one of the most common and virulent, resulting in large losses for the aquaculture industry. However, our understanding of the role of the gill microbiome during AGD development is limited. Thus, we undertook a longitudinal study with the main objective of...
Questions
Question (1)
Hello to the scientific community in Research Gate,
I got unexpected results during my latest DNA and RNA extraction . I had to perform a DNA precipitation step adding isopropanol and sodium acetate to a Tris-EDTA buffer solution containing a nucleic acid mixture, and centrifuging the solution. After including the isopropanol (1:1 vol./vol., at -20ºC) and the sodium acetate (1:10 vol./vol., at 4ºC), and centrifuge all samples (11,000 xG, 30 min, 4ºC) I realized that there was only 1 homogeneous layer in every tube. The two supposed non miscible liquids did not end up as 2 layers in the tube.
Do you know what could happen?
Do you think that the Tris-HCl in the Tris-EDTA Buffer could be the causative somehow?
Thank you very very much
Victor B. Birlanga
