Victor Blasco Birlanga

Victor Blasco Birlanga
National University of Ireland, Galway | NUI Galway · Department of Microbiology

Bachelor of Biology

Publications

Publication (1)
Preprint
Full-text available
Background Amongst gill disorders in Atlantic salmon, amoebic gill disease (AGD) is currently one of the most common and virulent, resulting in large losses for the aquaculture industry. However, our understanding of the role of the gill microbiome during AGD development is limited. Thus, we undertook a longitudinal study with the main objective of...

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Introduction
I describe the gill microbiome of Atlantic salmon from the West coast of Ireland, in relation with the Amoebig Gill Disease (AGD).

Questions

Question (1)
Question
Hello to the scientific community in Research Gate,
I got unexpected results during my latest DNA and RNA extraction . I had to perform a DNA precipitation step adding isopropanol and sodium acetate to a Tris-EDTA buffer solution containing a nucleic acid mixture, and centrifuging the solution. After including the isopropanol (1:1 vol./vol., at -20ºC) and the sodium acetate (1:10 vol./vol., at 4ºC), and centrifuge all samples (11,000 xG, 30 min, 4ºC) I realized that there was only 1 homogeneous layer in every tube. The two supposed non miscible liquids did not end up as 2 layers in the tube.
Do you know what could happen?
Do you think that the Tris-HCl in the Tris-EDTA Buffer could be the causative somehow?
Thank you very very much
Victor B. Birlanga

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Projects

Project (1)
Project
I describe the gill microbiome of Atlantic salmon from the West coast of Ireland, in relation with the Amoebig Gill Disease (AGD).