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Introduction
Vicki Doronina currently works at the Education faculty, Manchester Metropolitan University. Vicki does research in Cell Biology, Microbiology and Molecular Biology. Their current project is 'Yeast telomeres as a model for drug development'.
Additional affiliations
January 2011 - January 2014
Publications
Publications (20)
Mammalian and fungal prions arise de novo; however, the mechanism is poorly understood in molecular terms. One strong possibility is that oxidative damage to the non-prion form of a protein may be an important trigger influencing the formation of its heritable prion conformation. We have examined the oxidative stress-induced formation of the yeast...
Visualization of overexpression-induced Sup35 aggregates and the cortical actin cytoskeleton.
Fluorescence micrographs are shown for [PIN+][psi-] versions of the wild-type, abp1, crn1 and pan1 mutant strains containing the Sup35NM-GFP plasmid induced with copper for 24 hours. Rhodamine-phalloidin staining was used to visualize the cortical actin cy...
Proteins co-purifying with Sup35 in wild-type and tsa1 tsa2 mutant strains.
Sup35-TAP was immunoprecipitated from the wild-type and tsa1 tsa2 mutant strains and the associated proteins identified from three repeat experiments using mass spectrometry. This resulted in the identification of 63 and 47 proteins which are shown according to whether they...
Functional categorisation of Sup35-associated proteins.
The proteins identified within each MIPS category classification shown in Fig 1A are listed.
(XLSX)
Prions are self-propagating, infectious proteins that underlie several neurodegenerative diseases. The molecular basis underlying their sporadic formation is poorly understood. We show that autophagy protects against de novo formation of [PSI(+)], which is the prion form of the yeast Sup35 translation termination factor. Autophagy is a cellular deg...
Prions are self-perpetuating amyloid protein aggregates which underlie various neurodegenerative diseases in mammals and heritable traits in yeast. The molecular basis of how yeast and mammalian prions form spontaneously into infectious amyloid-like structures is poorly understood. We have explored the hypothesis that oxidative stress is a general...
The RNA component of signal recognition particle (SRP) is transcribed by RNA polymerase III, and most steps in SRP biogenesis
occur in the nucleolus. Here, we examine processing and quality control of the yeast SRP RNA (scR1). In common with other
pol III transcripts, scR1 terminates in a U-tract, and mature scR1 retains a U4–5 sequence at its 3′ e...
Expression of viral proteins frequently includes non-canonical decoding events (‘recoding’) during translation. ‘2A’ oligopeptides
drive one such event, termed ‘stop-carry on’ recoding. Nascent 2A peptides interact with the ribosomal exit tunnel to dictate
an unusual stop codon-independent termination of translation at the final Pro codon of 2A. Su...
Ribosomal progression through the open reading frames within mRNAs is frequently considered as uneventful when compared with the highly regulated initiation step. However, both RNA and nascent peptide can interact with the ribosome to influence how translation proceeds and can modify gene expression in several ways. 2A peptides are a class of seque...
Some RNA and protein sequences are capable of directing changes to the course of translation from that expected from the mRNA sequence, and this process is termed translational 'recoding'. 'CHYSEL' peptides are approximately 19-amino-acid sequences found in many viral genomes. When translated at internal portions of polypeptides, they yield co-tran...
“2A” oligopeptides are autonomous elements containing a D(V/I)EXNPGP motif at the C terminus. Protein synthesis from an open
reading frame containing an internal 2A coding sequence yields two separate polypeptides, corresponding to sequences up to
and including 2A and those downstream. We show that the 2A reaction occurs in the ribosomal peptidyltr...
Regulation of protein synthesis at the level of translation termination is a relatively underexplored, but rapidly expanding
field. Recent advances in elucidating the mechanism of translation termination are helping to understand noncanonical events
associated with translation termination. These “recording” events include read-through of stop codon...
Regulation of protein synthesis at translation termination is a relatively under-explored, but rapidly expanding field. Recent advances in elucidating the mechanism of translation termination are helping to understand non-canonical events associated with translation termination. These "recoding" events include read-through of stop-codons, insertion...
The endonuclease activity of EcoKI is regulated by the ClpXP-dependent degradation of the subunit that is essential for restriction, but not modification. We monitored proteolysis in mutants blocked at different steps in the restriction pathway. Mutations that prevent DNA translocation render EcoKI refractory to proteolysis, whereas those that perm...
ClpXP-dependent proteolysis has been implicated in the delayed detection of restriction activity after the acquisition of the genes (hsdR, hsdM, and hsdS) that specify EcoKI and EcoAI, representatives of two families of type I restriction and modification (R-M) systems. Modification, once established, has been assumed to provide adequate protection...