Véronique Rosilio

• PhD
• Professor (Full) at University of Paris-Saclay

Photodynamic therapies combining the action of a photosensitizer (PS), molecular oxygen, and light make it possible to destroy certain infectious sites and tumors. The incorporation of photosensitizers in nanocarriers allows for better control of their distribution in tissues and increases their concentration in the area that will be then illuminat...

In the present work, two photosensitizing drugs, Temoporfin and Verteporfin have been studied. Both have regular approval in Europe, Temoporfin for the treatment of head and neck cancers and Verteporfin for the treatment of age-related macular degeneration (AMD). The treatment modality, known as “Photodynamic Therapy” (PDT), involves drug activatio...

Phospholipid-porphyrin conjugates (PL-Por) are nowadays considered as a unique class of building blocks that can self-assemble into supramolecular structures that possess multifunctional properties and enhanced optoelectronics characteristics compared to their disassembled counterparts. However, despite their versatile properties, little is known a...

Multiple w/o/w emulsions (MEs) are promising systems for protecting fragile hydrophilic drugs and controlling their release. We explore the capacity of a single pH-sensitive copolymer, PDMS60-b-PDMAEMA50, and salts, to form and stabilize MEs loaded with sucrose or catechin by a one-step mechanical process or a microfluidic method. ME cytotoxicity w...

Phospholipid-porphyrin conjugates (Pl-Por) are nowadays considered as a unique class of building blocks that can self-assemble into supramolecular structures that possess multifunctional properties and enhanced optoelectronics characteristics compared to their monomeric counterparts. However, despite their versatile properties, little is known abou...

Hypothesis Phospholipid-porphyrin (Pl-Por) conjugates consist of porphyrin derivatives grafted to a lysophosphatidylcholine backbone. Owing to their structural similarities with phospholipids, Pl-Por conjugates can self-assemble into liposome-like assemblies. However, there is a significant lack of information concerning the impact of the porphyrin...

Background Porphyrin-lipids are versatile building blocks that enable cancer theranostics and have been applied to create several multimodal nanoparticle platforms, including liposome-like porphysome (aqueous-core), porphyrin nanodroplet (liquefied gas-core), and ultrasmall porphyrin lipoproteins. Here, we used porphyrin-lipid to stabilize the wate...

Lipid-based photoantimicrobial supramolecular assemblies are promising structures for the development of new photodynamic treatments of wound bacterial infections. Phenalenone (1H-phenalene-1-one, PN) has been used to obtain four stearoyl acid-based phenalenone derivatives (PNC18), with different types of linkage and different number of acyl chains...

Beta-lapachone (β-Lap) is an anticancer drug activated by the NAD(P)H quinone acceptor oxidoreductase (quinone) (NQO1), an enzyme over-expressed in a large variety of tumors. B-Lap is poorly soluble in water and in most biocompatible solvents. Micellar systems, liposomes and cyclodextrins (CDs) have been proposed for its solubilization. In this wor...

Beta-lapachone (β-Lap) is a promising anticancer drug whose applications have been limited so far due to its poor solubility and stability. Its encapsulation in liposomes has been proposed to overcome these issues. However, surface pressure measurements show that β-Lap exhibits an atypical interfacial behavior when mixed with lipids. Although the d...

Retinoblastoma is a malignant tumor of the retina in infants. Conventional therapies are associated to severe side effects and some of them induce secondary tumors. Photodynamic therapy (PDT) thus appears as a promising alternative as it is non-mutagenic and generates minimal side effects. The effectiveness of PDT requires the accumulation of a pho...

Therapeutic monoclonal antibodies (mAbs) are complex glycoproteins and ensuring their safety, efficacy and quality is still challenging. Indeed, during their manufacturing process, they are exposed to several stresses that can lead to their denaturation, misfolding or dimerization. We report here a new method based on capillary electrophoresis coup...

Light-responsive liposomes are considered nowadays as one of the most promising nanoparticulate systems for the delivery and release of an active pharmaceutical ingredient (API) in a spatio-temporal manner. Several strategies can be used to design photo-triggered liposomes. One of them consists in the incorporation of a photosensitizer (PS) in the...

Tetraphenylporphyrins (TPPs) have been proposed for the treatment of retinoblastoma by photodynamic therapy. Glycoconjugated compounds were synthesized for improving TPP solubility and amphipathy, and to specifically target mannose receptors overexpressed at the surface of cells. The efficiency of four TPP derivatives with different chemical struct...

In specific conditions, co-incubation of polymer nanoparticles and phospholipid vesicles leads to the formation of lipid bilayer coated nanoparticles usable as biocompatible drug delivery systems for co-encapsulation of two drugs. In this work, we focused on the preparation and characterization of liponanoparticles obtained by co-incubation of poly...

Lipid–porphyrin conjugates are considered as promising building blocks for supramolecular structures with multifunctional properties such as those required for anticancer photodynamic therapy. The Full Paper by A. Makky et al. on page 19179 ff. describes the synthesis of two new lipid‐porphyrin conjugates by coupling chlorophyll‐a derivative, pheop...

Lipid‐porphyrin conjugates are considered nowadays as promising building blocks for the conception of supramolecular structures with multifunctional properties, required for efficient cancer therapy by photodynamic therapy (PDT). Herein, we report on the synthesis of two new lipid‐porphyrin conjugates coupling pheophorbide‐a (Pheo‐a), a photosensit...

Pickering emulsions were formulated using biodegradable and biocompatible poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) prepared without surfactants or any other polymer than PLGA. A pharmaceutical and cosmetic oil (Miglyol) was chosen as the oil phase at a ratio of 10% w/w. These emulsions were then compared with emulsions using the sam...

Fourier Transform Infrared (FTIR) microspectroscopy was used to highlight the interactions between two photosensitisers (PS) of different geometries, TPPmOH4 and a glycoconjugated analogous, TPPDegMan, and lipid bilayers modelling retinoblastoma cell membranes. Retinoblastoma is a rare disease occurring in young infants, for whom conservative treat...

A new family of amphiphilic glycoconjugated porphyrin dimers usable in two-photon photodynamic therapy was designed, prepared and characterized. The physicochemical and biological data were presented and discussed. Their two-photon absorption cross section was determined, revealing quite good performances of the compounds (δ ≈ 300 GM at 880 nm for...

Cholecalciferol (D3) and retinyl palmitate (RP) are the two main fat-soluble vitamins found in foods from animal origin. It is assumed that they are solubilized in mixed micelles prior to their uptake by intestinal cells, but only scarce data are available on the relative efficiency of this process and the molecular interactions that govern it. The...

Lipid models such as monolayers, vesicles, and planar bilayers have been developed to better understand the physicochemical and biophysical properties of biological membranes. Today, they are used extensively to study protein-lipid and lipid-lipid interactions. They also allow to analyze the interaction of exogenous molecules or nanoparticles with...

The effect of high pressure homogenization (HPH) on the structure of β-lactoglobulin (β-lg) was studied by combining spectroscopic, chromatographic, and electrophoretic methods. The consequences of the resulting structure modifications on oil/water (O/W) interfacial properties were also assessed. Moderated HPH treatment (100 MPa/4 cycles) showed no...

In the context of the global prevalence of vitamin D insufficiency, we compared two key determinants of the bioavailability of 3 vitamin D forms with significant biopotencies: cholecalciferol, 25-hydroxycholecalciferol and 1-α-hydroxycholecalciferol. To this aim, we studied their incorporation into synthetic mixed micelles and their uptake by intes...

The outer membrane (OM) of Gram-negative bacteria is a complex and asymmetric bilayer that antimicrobial peptides must disrupt in order to provoke the cell lysis. The inner and external leaflets of the OM are mainly composed of phospholipids (PL), and lipopolysaccharide (LPS), respectively. Supported lipid bilayers are interesting model systems to...

Photo-triggerable liposomes are considered nowadays as promising drug delivery devices due to their potential to release the encapsulated drugs into a spatial and temporal manner. In this work, we have investigated the photopermeation efficiency of three photosensitizers (PSs), namely verteporfin, pheophorbide a and m-THPP when incorporated in lipo...

Since the first report of electroformed micrometer-sized liposomes in the 1980s, giant unilamellar vesicles (GUVs) have generated a lot of interest in the biophysical and biochemical communities. However, their penetration rate in high school or at the undergraduate level is still limited because of the requirement of specialized materials for thei...

Gram-negative bacteria possess a unique and complex cell envelope, composed of an inner and outer membrane separated by an intermediate cell wall-containing periplasm. This tripartite structure acts intrinsically as a significant biological barrier, often limiting the permeation of anti-infectives, and so preventing such drugs from reaching their t...

Phospholipid monolayers are often described as membrane models for analyzing drug-lipid interactions. In many works, a single phosphatidylcholine is chosen, sometimes with one or two additional components. Drug penetration is studied at 30mN/m, a surface pressure considered as corresponding to the pressure in bilayers, independently of the density...

Bacterial resistance to antibiotics has become today a major public health issue. In the development of new anti-infectious therapies, antimicrobial peptides appear as promising candidates. However, their mechanisms of action against bacterial membranes are still poorly understood. We describe for the first time the interaction and penetration of p...

This paper describes the measurement of the electroosmotic mobility (EOF) in a Wheatstone fluidic bridge (µFWB) as a direct probe of the surface instability. The variation of EOF known as one major contribution of the electrokinetic migration has been determined with a real time measurement platform after different conditionings on chips. We also s...

Hydrophobic organic compounds dissolved in a polar solvent can self-assemble into nanoparticles (NPs) upon nanoprecipitation into water. In the present study, we have investigated the structure of Squalenacetyl-adenosine (SQAc-Ad) nanoparticles which were previously found to exhibit impressive neuroprotective activity. When obtained by nanoprecipit...

An amphiphilic prodrug of gemcitabine, a cytidine analogue used in clinic against various tumours, had been previously synthesized by covalent coupling to squalene, a natural isoprenoid chain. The resulting bioconjugate self-assembled spontaneously in water as nanoparticles displaying an impressive activity both in vitro and in vivo. The aim of the...

Squalenoyl-gemcitabine (Sq-Gem) and squalenoyl-deoxycytidine (Sq-ddC) are amphiphilic prodrugs that self-assemble in water to form nanoassemblies (NAs) with well-defined structures and size. However, like other drug nanocarriers, these nanoassemblies are rapidly cleared from the blood stream by the reticulo-endothelial system. By adding squalenoyl-...

1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), a lipid comprising a saturated and an unsaturated acyl chain, belongs to the class of glycerophosphatidylcholines, major lipids in eukaryotic cell membranes. To get insight into the structural properties of this lipid within monolayers as membrane models, we performed molecular dynamics (MD)...

Squalene-based nucleolipids, including anticancer or antiviral prodrugs, gave rise to nanoparticles displaying a diversity of structures upon nanoprecipitation in water. Synchrotron small-angle X-ray scattering and cryo-TEM imaging revealed that both the nature of the nucleoside and the position of the squalene moiety relative to the nucleobase det...

Scope: Vitamin E is present in feed and food mainly as d-α-tocopherol (d-α-TOL) but also as all-rac-α-tocopheryl acetate (rac-α-TAC) through supplementation. Its absorption efficiency is low compared to that of triacylglycerols. The aim of this work was thus to study the fate of TAC during digestion. Methods and results: Using an in vitro digest...

The scale-up of nanoemulsions (NEs) produced by emulsification and solvent diffusion process was successfully achieved in the present work. Up to 1500 mL of NEs were produced with olive oil, castor oil, almond oil, or Arlamol™ E by using a Y-shaped mixer device. NE droplet sizes were significantly modulated from 290 to 185 nm by changing the proces...

Glycodendrimeric porphyrins seem promising photosensitizers usable in photodynamic therapy. Evidence of their ability to interact with an artificial supported bilayer membrane exhibiting a model sugar receptor has been previously shown. In the present work, the interaction of the glycodendrimeric porphyrins with retinoblastoma cells bearing the act...

Porphyrins are photosensitizers usable in photodynamic therapy. Although these molecules are clinically effective, their low water solubility and their lack of specificity are major drawbacks to their development. Our study was aimed at analysing the interfacial behaviour of glycoconjugated tetraphenylporphyrins newly synthesized at the Curie Insti...

In this paper, we discuss the evolution over the last 15 years in the Curie Institute of the concept, the development of the design and some properties of glycoconjugated photosensitizers with the aim to optimize the tumor targeting in photodynamic therapy. By this research, we have shown that specific interactions between a mannose-lectin and trim...

The surface of medical devices is of great importance for biocompatibility. Surface properties can evolve with a material treatment, time, and storage conditions. In this work, poly(urethane) catheters sterilised by cold nitrogen plasma treatment, were subjected to air and temperature aging in order to evaluate the influence of humidity and tempera...

Photodynamic therapy is a potentially efficient treatment for various solid tumours, among which retinoblastoma. Its efficacy depends on the preferential accumulation of photosensitizers in the malignant tissues and their accessibility to light. The specificity of drugs for retinoblastoma cells can be improved by targeting a mannose receptor overex...

Gemcitabine (dFdC or Gem) is a water-soluble cytotoxic drug, with poor cellular uptake in the absence of a nucleoside transporter. To improve its diffusion through membranes, it was modified by grafting of a squalenoyl moiety. In water, this derivative is able to form stable and monodispersed nanoparticles made of inverse hexagonal phases. The form...

Natural biopolymer stabilized oil-in-water emulsions were formulated using β-lactoglobulin (β-lg), gum arabic (GA), and β-lg:GA solutions as an alternative to synthetic surfactants. Emulsions using these biopolymers and their complexes were formulated varying the biopolymer total concentration, the protein-to-polysaccharide ratio, and the emulsific...

Three series of glycoconjugated and hydroxylated derivatives of 5,10,15,20-meso-tetraphenyl porphyrin (TPP) were studied in order to evaluate the effect of a porphyrin structure on its binding to dimyris- toylphosphatidylcholine (DMPC) liposomes and to human serum albumin (HSA). The studied derivatives have been developed as potent photosensitizers...

Polyurethane catheters made of Pellethane 2363-80AE® were treated with a low temperature plasma developed for the decontamination of reusable polymer devices in hospitals. We investigated the modifications of the polymer surface by studying the topographic modifications, the chemical modifications, and their consequences on the wettability and bact...

The rheological behavior and interfacial properties of olive oil–water emulsions stabilized by surfactant and clay particles (smectite) were studied to evaluate the effect of particles and surfactant distribution both in the bulk phase and at the oil–water interface. The temperature sweep of surfactant solutions and emulsions with and without clay...

The need for new products derived from natural sources for the replacement of the commonly used non-ionic surfactants containing ethylene oxide units with degradable carbohydrate headgroups has become an important area of research. Glycolipids offer a wide range of applications in the pharmaceutical and cosmetic fields and can compete with the most...

In photodynamic therapy, the specificity of a photosensitizer and its penetration into tumor cells are crucial. We have analyzed the ability of newly synthesized meso-(tetraphenyl)porphyrins to be recognized by a model of mannose-specific proteins overexpressed at the surface of retinoblastoma cells. The specific interaction of porphyrin with Con A...

Photodynamic therapy (PDT) is considered one efficient treatment against retinoblastoma. The specificity of a photosensitizer and its penetration into cancerous cells are crucial for achieving tumor necrosis. The selection of photosensitizers such as porphyrin derivatives by tumor cells thus depends to a large extent on their ability to interact wi...

The thermodynamic behavior of mixed floating monolayers of zwitterionic phospholipids and trans-dehydrocrotonin (t-DCTN) at different molar fractions (x) of the diterpene has been investigated by measuring the surface pressure and surface potential Langmuir isotherms. Excess Gibbs energies of mixing have been calculated for different compositions o...

Polyurethane (PU) catheters made of Pellethane 2363-80AE® were treated in two different ways: a new treatment with low temperature plasma that could be used to decontaminate reusable polymer devices in hospitals, and an e-beam (EB) irradiation. Polymer structure and bulk properties were studied by differential scanning calorimetry (DSC), thermograv...

We modulate precisely the surface morphology of polymer microcapsules containing perfluorooctyl bromide prepared by the solvent-evaporation technique by varying the percentage of PLGA-PEG copolymer in the formulation. As copolymer percentage increases, protrusions start to appear, become more numerous and finally fuse to yield sponge-like capsules....

The lipid droplets, in total parenteral nutrition admixtures, are composed of triglycerides surrounded by a phospholipid monolayer. Physicochemical interactions between lipids and the other components are critical for the emulsion stability. To assess these interactions at the molecular level, we have constructed a model of a mixed phospholipid mon...

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

The behaviour of olive oil-in-water emulsions (O/W) was studied in the presence of smectite particles. The distribution of these particles in the emulsions and the effect of their interaction with the surfactant on the stability of the emulsions were investigated. Whereas the variation of surfactant and/or clay content did not seem to affect the na...

Two glycodendrimeric phenylporphyrins were synthesized and their interaction with phospholipids was studied at the air-water interface and in liposome bilayers; such liposomes bearing glycodendrimeric porphyrin could constitute an efficient carrier for drug targeting in photodynamic therapy.

In a strategy to develop more stable ultrasound contrast agents (UCAs), we have designed a process to obtain nano/microcapsules with a single core of liquid perfluorocarbon within a biodegradable polymeric shell of homogeneous thickness. During the optimization of perfluorooctyl bromide (PFOB) encapsulation by solvent emulsion-evaporation, a marked...

The existence of inter- and/or intramolecular interactions in aqueous solutions of hydrophobically modified polysaccharides induces specific macromolecular conformations at the air–solution interface and in the bulk, where hydrophobic microdomains may be formed. The present work focuses on the interfacial and solubilizing properties of amphiphilic...

In multiple emulsion systems, oily or aqueous transfers may occur between the dispersed droplets through the continuous phase. These transfers are controlled by both the surfactant system (micellar transport), and the partial solubility of one phase in another (molecular transport). The latter could be anticipated from the knowledge of oil polarity...

This work was intended to develop and evaluate a new polymeric system based on amphiphilic carboxymethylpullulans (CMP(49)C(8) and CMP(12)C(8)) that can spontaneously self-assemble in aqueous solutions and efficiently solubilize hydrophobic drugs. The self-assembling properties of CMP(49)C(8) and CMP(12)C(8) were characterized by fluorescence spect...

The miscibility of two phospholipids dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylcholine (DMPC) possessing both a choline head group, with per(6-dodecanoylamino-6-deoxy) β-cyclodextrin (CIICONH-\-CD) and poly(ethylene oxide)-bearing lipid (PEO-lipid), respectively, has been assessed by surface pressure measurements of binary mo...

New nanoassemblies were instantaneously prepared by mixing two aqueous solutions, one containing a beta-cyclodextrin polymer (pbetaCD), and the other a hydrophobically modified by alkyl chains dextran (MD). The formation mechanism and the inner structure of these nanoassemblies were analysed using surface tension measurements and (1)H NMR spectrosc...

Fusogenic liposomes have a wide-range of applications as DDS and gene/protein delivery into living cells. A variety of surface modifications of drug carriers, to enable fusion with cells, have been proposed, however, the mechanism of fusion has still not been determined. To further improve the efficiency of drug carriers, a simple and easily examin...

This study describes the interactions between two amphiphilic molecules with antileishmanial activity, amphotericin B (AmB) and miltefosine [hexadecylphosphocholine (HePC)], the latter being effective by the oral route. The effect of HePC on the aggregation state of AmB in aqueous solution and the interactions between the two agents were monitored...

Nucleoside analogues display significant anticancer or antiviral activity by interfering with DNA synthesis. However, there are some serious restrictions to their use, including their rapid metabolism and the induction of resistance. We have discovered that the linkage of nucleoside analogues to squalene leads to amphiphilic molecules that self-org...

We show here, for the first time, that two neutral polymers may completely associate together in water to spontaneously form supramolecular nanoassemblies (nanogels) of spherical shape. The cohesion of these stable structures of about 200 nm is based upon a "lock and key" mechanism: inclusion complexes are formed between the hydrophobic alkyl chain...

The surface properties of the neoglycolipid (GlcNAcE(3)G(28)) and of its PEO-lipid (E(3)G(28)) moiety mixed with phospholipids (dipalmitoylphosphatidylcholine, DPPC; distearoylphosphatidylcholine, DSPC; diarachidoylphosphatidylcholine, DAPC; and dibehenoylphosphatidylcholine, DBPC) were studied in Langmuir monolayers at various mixture compositions...

The serotonergic system plays a critical role in a wide variety of physiological and behavioral processes. Dysregulation of the tightly controlled extracellular concentration of serotonin (5-hydroxytryptamine, 5-HT) appears to be at the origin of a host of metabolic and psychiatric disorders. Since the plasma membrane 5-HT transporter (SERT) is the...

The present article focuses on the comparative study of physicochemical properties of two ionic pullulan derivatives modified by 10 or 35 C8 chains per 100 anhydroglucose units, named CMP10C8 and CMP35C8, respectively. In aqueous solutions, these derivatives exhibited an associative behavior as evidenced by pyrene fluorescence spectroscopy. This ph...

The interfacial behavior of the hypoglycemia lectin BmoLL purified from the leaves of Bauhinia monandra, and its ability to interact with lipid monolayers has been studied by surface tension (γ) measurements. The results of these experiments revealed that in the solution concentration range comprised between 0.2 and 1.0 mg/ml, there was an extremel...

The use of porphyrin derivatives in photodynamic therapy is of excellent prospect for the treatment of superficial or easily reachable tumors. The selection of porphyrin derivatives by tumor cells depends to a large extent of their ability to interact with the biological membrane. The evaluation of porphyrin interaction with phospholipids can thus...

Insulin-loaded W/O/W multiple emulsions (ME) composed of medium-chain triglycerides have been shown to decrease the blood glucose level after oral administration to diabetic rats. Fish oil (very long-chain triglycerides) could be an alternative to medium-chain triglycerides because its chronic consumption has beneficial therapeutic effects. The aim...

W/O/W multiple emulsions are systems of potential interest in the oral administration of insulin. Although it has been shown that a single oral administration of insulin-loaded W/O/W multiple emulsion to diabetic rats led to the significant decrease of blood glucose levels (Silva Cunha et al., 1998, Int J Pharm 169:33), repeated administrations dis...

The interaction of three lectins (wheat germ, Ulex europaeus I, and Lotus tetragonolobus agglutinins: WGA, UEA-I and LTA) with either N-acetyl-D-glucosamine or L-fucose neoglycolipids incorporated into phospholipid monolayers and liposome bilayers was studied at the air/water interface and in bulk solution. The results show that for both systems st...

The effect of both lipid headgroup charge and of aqueous subphase pH on adsorption of glucose oxidase molecules (GOx) into dibehenoylphosphatidylcholine (DBPC) and octadecylamine (ODA) monolayers has been studied by surface tension and surface pressure measurements. The results revealed that whereas the extent of GOx adsorption into pure DBPC monol...

We have recently described how oligonucleotide (ON) stability and release from O/W cationic emulsions are governed by the lipid composition. The aim of the present paper was to investigate the properties of the ON/lipid complexes through fluorescence resonance energy transfer (FRET), size, surface tension measurements and cryomicroscopy. Starting f...

Whereas the adsorption of globular proteins has been extensively studied at the air/liquid interface, the interfacial behavior of intrinsic membrane proteins is still poorly known. The purified serotonin transporter (SERT), a 12-transmembrane-domain protein, is able to form a monolayer at the air/liquid interface. The organization of the protein at...

The serotonin transporter (SERT) is the target of several important antidepressant and psychostimulant drugs. It has been shown that under defined conditions, the transporter spread at the air/water interface was able to bind its specific ligands. In this paper, the interfacial organization of the protein has been assessed from dynamic surface pres...

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.