Veronica I Dodero- Ph.D.
- Principal Investigator at Bielefeld University
Veronica I Dodero
- Ph.D.
- Principal Investigator at Bielefeld University
About
72
Publications
42,343
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Introduction
Veronica does research in Chemical Biology, Biophysics, and Organic Chemistry. Her current projects are Functional Molecules, Nanosystems and the role of 33 mer gliadin aggregates as triggers of gluten-related disorders using fundamental knowledge at the interface between disciplines.
Current institution
Additional affiliations
March 2015 - October 2019
January 1998 - January 2018
Education
June 2010 - July 2012
March 2007 - November 2007
March 2003 - February 2007
Publications
Publications (72)
Gliadin, a protein present in wheat, rye and barley, undergoes incomplete enzymatic degradation during digestion, producing an immunogenic 33-mer peptide, LQLQPF(PQPQLPY)3 PQPQPF. The special features of 33-mer that provoke a break in its tolerance leading to gliadin sensitivity and celiac disease remains elusive. Herein it is reported that 33-mer...
We report the synthesis and characterization of a simple non-ionic azoamphiphile, C12OazoE3OH which behaves as an optically-controlled molecule alone and in a biomembrane-environment. First, Langmuir monolayer and Brewster angle microscopy (BAM) experiments showed that pure C12OazoE3OH enriched in the (E) isomer was able to form solid-like mesophas...
We obtained functional azovesicles based only on a simple nonionic azoamphiphile, C12OazoE3OH. The process combines evaporation-induced and solvent-induced directed assembly.
Gluten-related disorders are a complex group of diseases that involve the activation of the immune system triggered by the ingestion of gluten. Among these, celiac disease, with a prevalence of 1 %, is the most investigated, but recently, a new pathology, named nonceliac gluten sensitivity, was reported with a general prevalence of 7 %. Finally, th...
Gliadin, an immunogenic protein present in wheat, is not fully degraded by humans and after the normal gastric and pancreatic digestion, the immunodominant 33-mer gliadin peptide remains unprocessed. The 33-mer gliadin peptide is found in human faeces and urine, proving not only its proteolytic resistance in vivo but more importantly its transport...
Gut microbiota and immunity in health and disease: dysbiosis and eubiosis’s effects on the human body
The 33‐mer gliadin peptide and its deamidated derivative, known as 33‐mer DGP, are proteolytically resistant peptides central to the pathomechanism of celiac disease (CeD), the autoimmune presentation of gluten‐related disorders (GRD). Both peptides can form spontaneous oligomers in the nanomolar concentration, leading to the formation of nanostruc...
Editorial: Gut microbiota and immunity in health and disease: dysbiosis and eubiosis’s effects on the human body
Tauopathies are a group of neurodegenerative diseases characterized by the deposition of abnormal aggregates of tau protein into the brain. Tau is a microtubule-associated protein whose physiological role is related to the modulation of microtubule dynamics and the correct axonal transport in neurons. In tauopathies, the mechanism of conversion of...
The accumulation of intracellular aggregates of Tau protein is one main hallmark of Alzheimer's disease (AD) and is the consequence of Tau conformational changes, increased phosphorylation, and self-association to form fibrillar aggregates. This pathological process prevents the physiological interaction of Tau with microtubules to the detriment of...
Celiac disease (CeD) is an autoimmune disorder triggered by gluten proteins, affecting approximately 1 % of the global population. The 33‐mer deamidated gliadin peptide (DGP) is a metabolically modified wheat‐gluten superantigen for CeD. Here, we demonstrate that the 33‐mer DGP spontaneously assembles into oligomers with a diameter of approximately...
Celiac disease (CeD) is an autoimmune disorder triggered by gluten proteins, affecting approximately 1% of the global population. The 33‐mer deamidated gliadin peptide (DGP) is a metabolically modified wheat‐gluten superantigen for CeD. Here, we demonstrate that the 33‐mer DGP spontaneously assembles into oligomers with a diameter of approximately...
This policy brief aims to provide a comprehensive framework with key policy recommendations and actions that highlight the critical importance of addressing the health challenges faced by migrants and refugees in host countries. By addressing facilitators, barriers, frontline worker experiences, system integration, and specific healthcare needs, th...
The level of synergies between the human host and gut microbiota is frequently underestimated: growing evidence demonstrates a multi-faceted interplay influencing health status stability of the host, health-to-disease transition, disease severity grad, therapy-resistance and individual outcomes. Moreover, a powerful instrument has been established...
The Global Health and Migration Community was invited to submit a statement to the German Bundestag's Sub Committee on Global Health on shortages of the health workforce in Germany and its impact on global health dynamics. Sub Committee members appreciated the feedback, stating that the Community’s “in-depth analysis brought very valuable informati...
The 33-mer gliadin peptide and its deamidated metabolite, 33-mer DGP, are the immunodominant peptides responsible for the adaptive immune response in celiac disease (CD). CD is a complex autoimmune chronic disorder triggered by gluten ingestion that affects the small intestine and affects ∼1% of the global population. The 33-mers are polyproline II...
Gluten related‐disorders have a prevalence of 1–5 % worldwide triggered by the ingestion of gluten proteins in wheat, rye, barley, and some oats. In wheat gluten, the most studied protein is gliadin, whose immunodominant 33‐mer amino acid fragment remains after digestive proteolysis and accumulates in the gut mucosa. Here, we report the formation o...
Celiac Disease (CeD) is a chronic small intestinal immune-mediated enteropathy caused by the ingestion of dietary gluten proteins in genetically susceptible individuals. CeD is one of the most common autoimmune diseases, affecting around 1.4% of the population globally. To date, the only acceptable treatment for CeD is strict, lifelong adherence to...
Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR,...
This chapter presents a selection of multimodal (combined) methods for the integral investigation of different proteins/peptides and their aggregation or fibrillation process in three levels of complexity. The first level considers the in vitro scenario where isolated polypeptides are investigated by low-resolution and atomistic techniques. The sec...
The aggregation of proteins into amyloid fibers is linked to more than forty still incurable cellular and neurodegenerative diseases such as Parkinson's disease (PD), multiple system atrophy, Alzheimer's disease and type 2 diabetes, among others. The process of amyloid formation is a main feature of cell degeneration and disease pathogenesis. Despi...
In recent years, the evaluation of the structural properties of food has become of crucial importance in the understanding of food-related disorders. One of the most exciting systems is gliadin, a protein in wheat gluten, that plays a protagonist role in gluten-related disorders with a worldwide prevalence of 5%, including autoimmune celiac disease...
Zonulin is a physiological modulator of intercellular tight junctions, which upregulation is involved in several diseases like celiac disease (CeD). The polyQ gliadin fragment binds to the CXCR3 chemokine receptor that activates zonulin upregulation, leading to increased intestinal permeability in humans. Here, we report a general hypothesis based...
Gluten-related disorders (GRDs) are a group of diseases that involve the activation of the immune system triggered by the ingestion of gluten, with a worldwide prevalence of 5%. Among them, Celiac disease (CeD) is a T-cell-mediated autoimmune disease causing a plethora of symptoms from diarrhea and malabsorption to lymphoma. Even though GRDs have b...
Mol. Nutr. Food Res. 2021, 65, 202100200
DOI: 10.1002/mnfr.202100200
An integral physicochemical and functional approach demonstrated that pepsin gliadin digest forms spontaneously amyloid-type nanostructures whose interaction with the Caco-2 enterocyte cellular model induces the expression of selected mRNAs involved in the activation of immune cel...
Scope
Proteolysis-resistant gliadin peptides are intensely investigated in biomedical research related to Celiac Disease and gluten-related disorders. Herein, the first integrated supramolecular investigation of pepsin-digested gliadin peptides, p-gliadin, is presented in combination with its functional behavior in Caco-2 cell line.
Methods and re...
![Parent peptides Ac‐klvff‐NH2 1 and H‐GVVIA‐NH2 2.[17]](publication/350741611/figure/fig1/AS:11431281245189171@1716113649460/Parent-peptides-Ac-klvff-NH2-1-and-H-GVVIA-NH2-217_Q320.jpg)
In peptidotriazolamers every second peptide bond is replaced by a 1H‐1,2,3‐triazole. Such foldamers are expected to bridge the gap in molecular weight between small‐molecule drugs and protein‐based drugs. Amyloid β (Aβ) aggregates play an important role in Alzheimer's disease. We studied the impact of amide bond replacements by 1,4‐disubstituted 1H...
Gliadin is a protein in wheat gluten that is not fully degraded during human digestion. The remaining large undigested peptides produce diverse diseases
affecting around 5% of the general population. The complicated relationship between food properties, behavior during digestion, and their contribution to human nutrition and health are not well un...
Indigoids represent natural product‐based compounds applicable as organic semiconductors and photoresponsive materials. Yet modified indigo derivatives are difficult to access by chemical synthesis. A biocatalytic approach applying several consecutive selective C−H functionalizations was developed that selectively provides access to various indigoi...
The self-assembly of proteins is an essential process for a variety of cellular functions including cell respiration, mobility and division. On the other hand, protein or peptide misfolding and aggregation is related to the development of Parkinson's disease and Alzheimer's disease, among other aggregopathies. As a consequence, significant research...
Cyclic RGD peptides are well‐known ligands of integrins. The integrins αVβ3 and α5β1 are involved in angiogenesis, and integrin αVβ3 is abundantly present on cancer cells, thus representing a therapeutic target. Hence, synthetic and biophysical studies continuously are being directed towards the understanding of ligand‐integrin interaction. In this...
Misfolding and aggregation of amyloid β1–42 peptide (Aβ1–42) play a central role in the pathogenesis of Alzheimer's disease (AD). Targeting the highly cytotoxic oligomeric species formed during the early stages of the aggregation process represents a promising therapeutic strategy to reduce the toxicity associated with Aβ1–42. Currently, the thiofl...
Azobenzenes are photoswitchable molecules capable of generating significant structural changes upon E- to- Z photoisomerization in peptides or small molecules, thereby controlling geometry and functionality. E- to- Z photoisomerization usually is achieved upon irradiation at 350 nm (π–π* transition), while the Z- to- E isomerization proceeds photoc...
Celiac disease (CeD) is a highly prevalent chronic immune‐mediated enteropathy developed in genetically predisposed individuals after ingestion of a group of wheat proteins (called gliadins and glutenins). The 13mer α‐gliadin peptide, p31‐43, induces proinflammatory responses, observed by in vitro assays and animal models, that may contribute to in...
The proteolytical resistant 33-mer gliadin peptide is the immunodominant fragment in gluten and responsible for celiac disease, and other gluten-related disorders. Meanwhile, the primary structure of 33-mer is associated with the adaptive immune response in celiac patients, the structural transformation of 33-mer into protofilaments activates a pri...
Transcription factors are proteins lying at the endpoint of signaling pathways that control the complex process of DNA transcription. Typically, they are structurally disordered in the inactive state, but in response to an external stimulus, like a suitable ligand, they change their conformation, thereby activating DNA transcription in a spatiotemp...
Peptide structural transformation and aggregation is associated with a large number of outsider aetiology diseases, and it is intrinsically linked to amyloid peptide aggregation. Diphenylalanine self-assembled structures are used as robust minimalist beta amyloids not only to elucidate protein aggregation but also to generate hydrogels. Herein, we...
Alpha-gliadin is a highly immunogenic protein from wheat, which is associated with many human diseases, like celiac disease and non-celiac gluten sensitivity. Because of that, gliadin solution is subject to intense biomedical research. However, the physicochemical nature of the employed gliadin solution at physiological pH is not understood. Herein...
A new hypothesis about the relation of gliadin and its immunodominant 33-mer fragment aggregates as triggers of gluten-related disorders was presented.
The 33-mer gliadin peptide, LQLQPF(PQPQLPY)3PQPQPF, is a highly immunogenic peptide involved in celiac disease and probably in other immunopathologies associated with gliadin. Herein, dynamic light scattering measurements showed that 33-mer, in the micromolar concentration range, forms polydisperse nano- and micrometer range particles in aqueous me...
We present the results of a study of outdoor air quality in two comparable regions of Buenos Aires province (Argentina); La Plata and Bahía Blanca, developed jointly by researchers of the National University in both cities, and of the Hospital of Bahía Blanca, between 2009 and 2011. Both regions are characterized by a large petrochemical complex an...
Medicinal chemistry is intimately connected with basic science such as organic synthesis, chemical biology and biophysical chemistry among other disciplines. The reason of such connections is due to the power of organic synthesis to provide designed molecules; chemical biology to give tools to discover biological and/or pathological pathways and bi...
In the present review, the chemistry of dyes as well as the interaction mechanisms between tissue and dye has been detailed, and also some of the key factors affecting the selectivity of dyes by certain cellular structures have been mentioned. Moreover, due to the relevance that histological stains have acquired in biomedical research, some of the...
One of the strategies used by nature to regulate gene expression relies on the stimuli-controlled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be...
Gliadin, a protein present in wheat, rye and barley is not fully degraded by humans, leading to allergies, gluten sensitivity and celiac disease.(1,2,3).It has been hypothesized that increased intestinal permeability is an early event in celiac disease pathogenesis (4) but it is completely unknown what endows gliadin the unusual proteolytic resista...
This chapter provides a general overview of several spectroscopic techniques currently available for studying protein secondary structure in solution and assessing its changes in response to ligand and/or environmental interaction. These techniques include ultraviolet—visible (UV—vis) absorption spectroscopy, circular dichroism (CD) spectroscopy, f...
The new non-ionic amphiphiles 2, 3 and 4 presented interesting thermotropic liquid crystal (LC) behavior as determinate by Differential Scanning Calorimetry (DSC) and Optical Polarizing Microscopy (POM). Compound 2 exhibited a non-cooperative phase transition presenting a nematic mesophase. Compound 3 and 4 showed a smectic polymorphic behavior. In...
The gel properties of ovalbumin mixtures with three different surfactants (sodium perfluorooctanoate, sodium octanoate and sodium dodecanoate) have been studied by rheological techniques. The gel elasticities were determined as a function of surfactant concentration and surfactant type. The fractal dimension of the formed structures was evaluated f...
In this review, we shall outline the basic principles of circular dichroism (CD) indicating the types of structural information relevant to the study of biomolecules, such as proteins or DNA. We are mainly interested to show the utility of this technique to study protein-ligand, DNA-ligand and protein-DNA interactions.
Synthesis and characterization of new amphiphilic azobenzenes with non ionic character is presented. The synthetic methodology is simple and versatile which allows the generation of molecular diversity from the same precursors in an easy way.
For Abstract see ChemInform Abstract in Full Text.
In the field of bioscience, the study of the interactions between blood proteins and fluorinated materials is very important from both theoretical and applied points of view. Fluorinated materials have potential use in drug delivery, as blood substitutes, and in biotechnology. Using a combination of ultraviolet-visible (UV-vis) and ultraviolet-circ...
The interaction between human immunoglobulin G (IgG) and sodium perfluorooctanate (SPFO) has been characterized by a combination of UV-vis and UV-CD spectroscopies and ion selective electrodes. The study determined that there were true specific unions between SPFO and IgG. The conformational changes at the bulk solution, induced by SPFO, were well...
In den Fußstapfen: Wenn bZIP-Peptide der basischen Region mit einer nichtkovalenten Heterodimerisierungseinheit versehen sind, lässt sich ihre Fähigkeit, in der großen DNA-Furche zu binden, durch Zugabe eines geeigneten externen Liganden auslösen, der in benachbarten kleinen Furchen bindet. Die so gebildeten kovalenten Hybride binden relativ lange...
The intrinsic recognition code associated with dsDNA allows either accelerating or retarding of a native chemical ligation reaction between tripyrrole ligands. The rate changes most probably stem from the sequence-dependent characteristics of the dsDNA-ligand complexes.
The interfacial behavior of mixed human serum albumin (HSA)/sodium perfluorooctanoate (C8FONa) solutions is examined by using two experimental techniques, pendant drop tensiometry and circular dichroism spectroscopy. Through the analysis of the surface tension of the mixed solutions, surface competitive adsorption at the air–water interface between...
This paper reports the results obtained in a study on the radical hydrostannation of mono- and disubstituted alkynes with bulky triorganotin hydrides using triethylborane as initiator. The addition of trineophyl- (1), tris[(phenyldimethylsilyl)methyl]- (2), and 9-tripticyldimethyltin (3) hydride to eight alkynes was carried out at room temperature...
The interaction of a fluorinated surfactant, sodium perfluorooctanoate, with human serum albumin (HSA) has been investigated by a combination of ultraviolet–circular dichroism (UV-CD) spectroscopy and potentiometry (by a home-built ion-selective electrode) techniques to detect and characterize the conformational transitions of HSA. By using differe...
The synthesis of the new compounds 9-triptycyldimethyltin bromide (2) and 9-triptycyldimethyltin hydride (3) and some of their physical properties are described. The radical addition of 3 to methyl (E)-2,3-diphenylpropenoate gave the threo adducts as the only products. The results obtained in the addition of hydride 3 to mono- and disubstituted alk...
The synthesis of the new bis[(phenyldimethylsilyl)methyl]tin dihydride (6) starting from chloromethyldimethylsilicon chloride is reported. A study on the radical additions of 6 to various mono- and disubstituted alkynes shows that these reactions take place with high and in some cases with complete stereoselectivity. Full 1H-, 13C-, and 119Sn-NMR d...
A study on the addition of trineophyltin hydride (1) to alkynones under free radical (AIBN and Et3B) and palladium-catalyzed [(PPh3)2PdCl2] conditions is reported. The results obtained indicate that the addition of 1 to eight ynones catalyzed by bis(triphenylphosphine)palladium(II) chloride led in all cases to addition products in very high yields...
A study on the addition of trimethyl-, tri-n-butyl-, triphenyl-, and (-)-menthyldimethyltin lithium to (R)-(+)-pulegone (5) and (E)-benzalcyclohexanone (8) is reported. It was found that the conjugate addition of the organotin anions to 5 lead in the four cases to mixtures of two adducts in good yields (70-80%) and satisfactory diastereomeric exces...
The synthesis of the new bulky tris[(phenyldimethylsilyl)methyl]tin hydride (9), tetrakis[(phenyldimethylsilyl)methyl]tin (5), and hexakis[(phenyldimethylsilyl)methyl]ditin (10) as well as some physical properties are described. The addition of hydride 9 to seven mono- and disubstituted alkynes under radical conditions indicates that these reaction...
Hydrostannation of mono- and disubstituted alkynes with trineophyltin hydride (1) leads to vinylstannanes in good to excellent yields, the configuration of the products depending on the reaction conditions. Thus. whereas hydrostannation. under radical conditions leads stereoselectively to only one of the two possible products corresponding to an an...
The syntheses of trineophyl- (1a) and tri-(-)-menthylstannyl phenylacetylene (1b) as well as that of (E)-1-trineophylestannyl-2-phenylethene (2) and (E)-1-trineophylstannyl-1,2-diphenylethene (3) are described. The hydrostannation of 1a with an excess of trimethyltin hydride led to 1,1,1-tris(trimethyltin)-2-phenylethane (4) and/or 1,1-bis(trimethy...
Questions
Question (1)
Dear Colleagues,
Guest Editor of the Special Issue of Molecules (IF 3.060) “Understanding Protein/Peptide Self-Assembly using Structural and Biophysical Chemistry”, it is my pleasure to invite you to submit an article on this topic.
The article may be either a full paper or a communication based on your own research in this area or maybe a focused review article on some aspect of the subject. All submissions will be subject to peer review. If you plan to submit a review article, please provide me with a title and brief description at your earliest convenience, in order to avoid multiple reviews covering the same material.
Special Issue: Understanding Protein/Peptide Self-Assembly using Structural and Biophysical Chemistry
https://www.mdpi.com/journal/molecules/special_issues/Protein_Peptide_Self-Assembly
Submission deadline: 30 April 2020.
Molecules is fully open access. Open access (unlimited and free access by readers) increases publicity and promotes more frequent citations, as indicated by several studies. Open access is supported by the authors and their institutes. An article-processing charge of CHF 1800 (APC) applies to each accepted paper. Note that for papers submitted after 31 December 2019, an APC of 2000 CHF will apply. You may be entitled to a discount if you have previously received a discount code.
I would appreciate if you could let us know if you are interested in preparing a manuscript for this Special Issue and I will send you more detailed instructions for submission.
I look forward to hearing from you at the earliest opportunity.
With best wishes,
Veronica Dodero
