
Veena AgrawalUniversity of Delhi | DU · Department of Botony
Veena Agrawal
Professor
About
103
Publications
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Introduction
Prof. Veena Agrawal, Senior Professor is specialized in Plant biotechnology,(Tissue culture and genetic transformation); nanobiotechnology; sex-linked molecular markers and genetic diversity diversity analysis; Medicinal plants research ( natural plant bioactive molecues (isolation, elicitation and bioefficacy of anticancerous, antidiabetic, larvicidal bioactive compounds); alleviation of metal-induced phytotoxicity employing antioxidants, glutathione and symbiotic fungus Piriformospora indica.
Publications
Publications (103)
Gentiana kurroo Royle, a critically endangered Himalayan herb, is valued in treating leucoderma, syphilis, bronchial asthma, hepatitis, etc. The current investigation performed quantitative and qualitative phytochemical analysis of G. kurroo root extracts prepared in chloroform, methanol, and ethyl acetate. The phenolic and flavonoid contents were...
This book is a comprehensive review of secondary metabolite production from plant tissue culture. The editors have compiled 12 meticulously organized chapters that provide the relevant theoretical and practical frameworks in this subject using empirical research findings. The goal of the book is to explain the rationale behind in vitro production o...
Nodal explants of Holarrhena pubescens, an important medicinal tree, were cultured on Murashige and Skoog’s medium (MS) containing 15 µM BA (control) alone and on medium supplemented with different concentrations (0, 1, 5, 25, 50, 100 and 200 mg/L) of heavy metals such as NiCl2, CoCl2, As2O3 and CrO3 to study their toxic effect. After 28 days of tr...
Rumex hastatus D. Don, a traditional medicinal plant is known for its antitumorous, antiangiogenic and antibacterial potential. HPLC analysis of different plant parts showed that root had the maximum quantity of luteolin and rutin and thus was selected for the establishment of aseptic cultures. Murashige and Skoog’s medium supplemented with 5 µM TD...
Rumex hastatus D. Don, a traditional medicinal plant known for its, antitumorous, antiangiogenic and antibacterial potential is continuously under threat of extinction due to over exploitation and climatic changes. Phytochemical analysis of different plant parts showed that roots had the maximum quantity of two bioactive molecules, luteolin and rut...
Atropa acuminata Royle ex Lindl. (family Solanaceae) is a critically endangered therapeutic herb, known for its immense therapeutic potential against innumerable ailments. In view of protecting this species from brink of extinction, present work focuses upon development of an effectual micropropagation protocol via indirect organogenesis using root...
Chitrak (Plumbago zeylanica L.), a renowned traditional medicinal plant, is being exploited extensively for its roots which are employed in the preparations of many important herbal products (e.g. Dashmularisht, Chitrakadivati) possessing anticancer, anti-atherogenic, cardiotonic, hepatoprotective and neuroprotective properties. P. zeylanica roots,...
Key message:
Atropa acuminata aqueous leaf extract biosynthesized silver nanoparticles showed strong antioxidant, anticancerous (HeLa cells) and anti-inflammatory activities. Besides, this bio syn-AgNP also proved effective against mosquito vectors causing malaria, dengue and filariasis. Present study highlights eco-friendly and sustainable approa...
Mosquitoes spread several life-threatening diseases such as malaria, filaria, dengue, Japanese encephalitis, West Nile fever, chikungunya, and yellow fever and are associated with millions of deaths every year across the world. However, insecticides of synthetic origin are conventionally used for controlling various vector-borne diseases but they h...
Development of therapeutic drugs for mosquito control and cancer is the need of the hour. This study highlights the fabrication of silver nanoparticles by incubating 45 ml of AgNO3 (0.5 mM) solution with 5 ml of Cullen corylifolium aqueous green seed extract at 60 ± 2 °C, 9 pH for 90 min. Ultraviolet–visible spectroscopy confirmed the synthesis of...
The present study encompasses green synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Arabian Primrose within 6 min of reaction at 60 °C, pH 7 and their characterisation using physico-chemical analytical techniques. UV-Visible spectroscopy elucidated the surface plasmon resonance around 420 nm. FESEM and TEM images revealed th...
Cullen corylifolium (L.) Medik. (Babachi), a traditional herb is being utilized to cure several serious ailments such as psoriasis, leprosy, leucoderma, osteoporosis including leukemia due to the occurrence of many important furanocoumarins and flavonoids especially in the green seeds. Since these compounds occur in extremely low amounts, in vitro...
The present investigation highlights the strong antioxidant, anticancer and larvicidal potential of green synthesized
silver nanoparticles (AgNPs) using aqueous leaf extract of Piper longum L. for their diverse therapeutic
applications. The optimum conditions for the synthesis of AgNPs were recorded as 1mM AgNO3, 60 ± 2°C at
pH 6 for 120 min. Synth...
Twenty inter-simple sequence repeat (ISSR) and twenty two start codon targeted (SCoT) primers were employed to analyze genetic diversity and population structure among 52 Trichosanthes dioica Roxb. accessions collected from nine different eco-geographical regions of India. ISSR markers proved to be more informative in genetic diversity assessment a...
The present study reports the biosynthesis of silver nanoparticles using aqueous root extract of Arnebia hispidissima. They were prepared by adding 10 mL root extract in 90 mL silver nitrate (0.5 mM) solution and heating at 60 ± 2 °C for 12 min at pH 7.5. Characterization of the biosynthesized silver nanoparticles was done using UV-Visible spectros...
Research Plate of Published work was picked up as Cover page of the Journal In vitro Cee Developmental Biol. -Plant Vol 54
Micropropagation and elicitation of plumbagin in root callus of Plumbago zeylanicum
Present investigation reveals copper induced phytotoxicity, oxidative stress and DNA damage in Cassia angustifolia Vahl and its amelioration by employing a symbiotic fungus, Piriformospora indica. Seeds were germinated on Knop's medium containing five Cu levels (0, 1, 10, 50, 100 and 200 mg L-1), with and without P. indica. Colonization with P. ind...
In vitro elicitation of an important compound conessine has been done in the bark-derived callus culture of Holarrhena antidysenterica (L.) Wall. employing different elicitors. For induction of callus, green bark explants excised from field-grown plants were cultured on MS medium augmented with different concentrations (0, 1, 2.5, 5, and 10 μM) of...
Plumbago zeylanica L., an important medicinal herb, possesses plumbagin, a valuable secondary metabolite. Roots of this plant, collected from four locations in Himachal Pradesh, India, were screened for plumbagin content with high-performance liquid chromatography. The chemotype collected from Hamirpur yielded the highest content (26.47 ± 0.63 mg g...
The present study was carried out to evaluate the larvicidal potential of methanol, hexane, acetone, chloroform, and aqueous bark extracts of Holarrhena antidysenterica (L.) Wall. and silver nanoparticles (AgNPs) synthesized using aqueous bark extract against the third instar larvae of Aedes aegypti L. and Culex quinquefasciatus Say. AgNPs were pre...
The present study highlights the strong larvicidal potential of silver nanoparticles (AgNPs) synthesized using bark extract of Holarrhena antidysenterica against third instar larvae of Anopheles stephensi over the other bark extract prepared in chloroform, hexane, ethyl acetate, methanol, aqueous and acetone individually. AgNPs were prepared by mix...
Nerium oleander is an ornamental species of high aesthetic value, grown in arid and semi-arid regions because of its drought tolerance, which is also considered as relatively resistant to salt; yet the biochemical and molecular mechanisms underlying oleander’s stress tolerance remain largely unknown. To investigate these mechanisms, one-year-old ol...
This study was conducted through the pot experiments to understand the mechanism of lead (Pb) and arsenic (As)-induced phytotoxicity and their possible alleviation by glutathione (GSH) and citric acid (CA) in two cultivars of Solanum lycopersicum L., i.e., Pusa ruby (PR) and Arka vikas (S22). Therefore, tomato seedlings were germinated in soil-rite...
Simarouba glauca DC. is an evergreen, polygamodioecious, eco-friendly tree borne oil seed crop. It is native of tropical rainforest regions of South and Central America and was first introduced in India in 1966. During present investigation, fourteen inter-simple sequence repeat (ISSR) and eleven sequence-related amplified polymorphism (SRAP) marke...
Elucidating the mechanisms of abiotic stress tolerance in different species will help to develop more resistant plant varieties, contributing to improve agricultural production in a climate change scenario. Basic responses to salt stress, dependent on osmolyte accumulation and activation of antioxidant systems, have been studied in Nerium oleander,...
Background:
Glioblastoma has been reckoned as the prime cause of death due to brain tumours, being the most invasive and lethal. Available treatment options, i.e. surgery, radiotherapy, chemotherapy and targeted therapies are not effective in improving prognosis, so an alternate therapy is insistent. Plant based drugs are efficient due to their sy...
As nanotechnology is expanding to several commercial fields, there is a need of ecofriendly and energy-efficient methods for the synthesis of nanoparticles. Algae have been discovered to reduce metal ions and subsequently for the biosynthesis of nanoparticles. Since algae-mediated biosynthesis of nanoparticles is an ecofriendly, economical, high-yi...
Jojoba, [Simmondsia chinensis (Link) Schneider] is a commercially important dioecious, desert shrub which is mainly cultivated for liquid wax (oil) present in its seeds (40–60 %). The oil is being utilized by the cosmetic, pharmaceuticals, and other industries for certain formulations. In this study, gender based differences in biochemical and phys...
Cassia angustifolia Vahl, a chief source of anthraquinone glycosides (sennosides), extensively employed as a laxative is also reported to possess significant anticancerous activity against various cancer cell lines. HPLC analysis of different in vivo plant parts viz., leaves, nodes, roots and seeds revealed that the maximum content of both sennosid...
Investigations conducted on Cassia angustifolia to see the effect of zinc (Zn) and copper (Cu) during seed germination, revealed drastic toxicity on seedling growth, alteration in antioxidant enzymes, morphological changes and metal accumulation in various plant parts. Seeds were germinated on Knop’s medium containing Zn and Cu individually in vari...
Salinity stress has been reckoned as one of the major threat towards crop productivity as it causes significant decline in the yield. The impact of NaCl stress (0, 1, 10, 50, 100 and 200 mg L(-1)) as well as glutathione (10 mg L(-1)) either alone or in combination has been evaluated on the induction of multiple shoots, antioxidant enzymes' activity...
CONTEXT/BACKGROUND
Adolescence, meaning “To grow up,” is a transitional stage of physical, sexual and mental human development occurring between
puberty and legal adulthood. The Government of India in National Youth Policy defines adolescents as individuals between 13 to 19
yrs. of age.
AIMS
To study the magnitude of different adolescent gyne...
To detect genetic variations among different Simmondsia chinensis genotypes, two gene targeted markers, start codon targeted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) were employed in terms of their informativeness and efficiency in analyzing genetic relationships among different genotypes. A total of 15 SCoT and 17 CBDP primers...
CONTEXT/BACKGROUND:
Preterm birth refers to a birth that occurs before 37
completed weeks
(
Abstract. Medicinal plants are nearly unlimited source of phytochemicals. Centella asiatica L. is a tropical medicinal plant with a long history of therapeutic uses, dermal disorders, venous insufficiency micro-angiopathy, and anticancer. The present study highlights (i) in vivo evaluation of asiatic acid (as an anticancer compound) content in diff...
Context: Psoralea corylifolia L. (Fabacese) is rich source of bioactive compounds, which endows
the plant with immense value for its use in pharmaceuticals, health, and body-care products.
Objective: The current study was designed (i) for the determination of psoralen from callus
derived from different plant parts, and (ii) for the enhancement of p...
A high frequency secondary somatic embryogenesis and plant regeneration have been achieved in Calliandra tweedii (Benth.) through internodal segments excised from 5-year-old shrub on Murashige and Skoog (MS) medium enriched with different concentrations of 2-isopentenyl adenine (2iP). Somatic embryos were differentiated directly from cut ends, epid...
In vitro protocol has been established for clonal propagation of Cassia angustifolia Vahl which is an important source of anticancerous bioactive compounds, sennoside A and B. Nodal explants excised from field raised elite plant (showing optimum level of sennoside A and B) of C. angustifolia when reared on Murashige and Skoog's medium augmented wit...
Flowering plants are known to exhibit vast
diversity of sexual systems encompassing bisexual,
monoecious and dioecious conditions. Dioecy offers
opportunities to explore separately the male and female
programmes giving an insight to the evolutionary,
developmental and molecular processes leading to
separate mechanisms for sex expression. Mechanisms...
There is a recent surge in the marker-assisted selection of desired sex type among economically important dioecious crops. Simmondsia chinensis (Jojoba), a dioecious crop is of immense agricultural importance where only the female plants are preferred for commerce. DNA fingerprinting technology, ISSR analysis along with bulk segregant analysis (BSA...
DNA fingerprinting studies have been carried out with the physiologically mature male and female plants of Jojoba using 80 ISSR primers with a view to generate sex-linked markers. After bulk segregant analysis, two unique ISSR markers, viz. ISSR848(1500) and VIS11(1317) have been developed which can be used for determining the sex at the seedling s...
Cullen corylifolium (L.) Medik. is a traditional herb of immense medicinal value. In vitro
micropropagation of an elite chemotype of C. corylifolium was achieved using nodal
explants employing different growth regulators (BA, Kn and 2iP). BA at 5 µM proved
optimum to differentiate the maximum average number of multiple shoots. Indirect
regenera...
In vitro mass production and conservation of three antimalarial medicinal herbs: Spilanthes acmella L. var. oleraceae, S. calva, and S. paniculata of asteraceae employing seedling leaf explants. The explants were reared on MS medium containing a variety of growth regulators, namely cytokinins (BA, KN and 2iP), or auxins (2,4-D, NAA and IBA) in diff...
Psoralea corylifolia, an important medicinal herb of Fabaceae is a rich source of secondary metabolites, furanocoumarins (psoralen, isopsoralen) and isoflavonoids (genistein, daidzein). A wide variation in terms of genetic diversity and psoralen content has been recorded through RAPD and HPLC analysis, respectively among various chemotypes of P. co...
Malaria and dengue are the two most important vector-borne human diseases caused by mosquito vectors Anopheles stephensi and Aedes aegypti, respectively. Of the various strategies adopted for eliminating these diseases, controlling of vectors through herbs has been reckoned as one of the important measures for preventing their resurgence. Artemisia...
In vitro techniques have been perfected for direct multiple shoot induction through cotyledonary node explants for the first time in Jatropha curcas, an important biodiesel plant. Seedling derived cotyledonary nodes when reared on MS medium augmented with 2 mg/l benzyladenine, 20 mg/l glutathione, 20 mg/l adenine sulphate and 10 mg/l silver nitrate...
An efficient in vitro protocol has been established for clonal propagation of elite plant of Spilanthes calva DC., an important source of spilanthol, an antimalarial larvicidal compound. Nodal explants excised from field grown plant of S. calva DC. when reared on Murashige and Skoog's medium augmented with different cytokinins, viz. N6-Benzyladenin...
Silver nanoparticles (Ag-NPs) have been synthesized at room temperature following a green approach by reducing the corresponding salt with tannic acid. The reaction was instantaneous and the average diameter of the particles formed was around 12 nm as measured by TEM. Electron diffraction pattern showed that the particles were crystalline in nature...
A significant enhancement in artemisinin content, an important anti-malarial compound, has been achieved in Artemisia annua L. shoots by co-cultivating with Piriformospora indica, a mycorrhiza-like fungus. The in vitro shoots derived from nodal cultures of A. annua were implanted on four different culture media namely, (i) Murashige & Skoog (MS) ba...
Bioefficacy of the three Spilanthes spp. were evaluated using their crude flower head extracts against malaria (An. stephensi Liston, An. culicifacies species C) and filarial (Cx. quinquefasciatus Say) mosquito vectors. Cent per mortality were achieved against the last III/ early IVth instar larvae of all the three mosquito spp. within 24 h of expo...
Diabetes is a major global epidemic currently affecting 50 million people across the world and is expected to rise to 300 million by 2025. However, the present pharmacological approach for the management of this serious disease is far from ideal, which behooves the exploration of newer and safer agents. Currently, there is an enormous increase in t...
Present investigation highlights the evaluation of an important but less explored anticancerous bioactive compound, psoralen from Psoralea corylifolia. HPLC analysis of in vivo plant parts such as roots, nodes and leaves revealed that the quantity of psoralen was optimum in nodal parts. In vitro enhanced biosynthesis of psoralen was achieved in the...
In vitro techniques have been perfected for direct multiple shoot induction through cotyledonary node expiants for the first time in Jatropha curcas L., an important biodiesel plant. Seedling derived cotyledonary nodes when reared on MS medium augmented with 2 mg/1 benzyladenine, 20 mg/1 glutathione, 20 mg/1 adenine sulphate and 10 mg/1 silver nitr...
Using in vitro bioassays, we evaluated the crude methanol, ethanol, chloroform, and acetone leaf extracts of Artemisia annua as well as pure artemisinin against late 3rd and early 4th instars of Culex quinquefasciatus and Cx. tritaeniorhynchus. Based on the lethal concentration for 50% mortality (LC50) and lethal concentration for 90% mortality (LC...
Psoralen were evaluated from in vivo and in vitro plant parts of Psoralea corylifolia by High
Performance Liquid Chromatography (HPLC). The high amount of psoralen (3038 μg/g fresh weight) in in
vivo condition were obtained in brown seeds. Psoralen content in plant parts growing in vitro were lower than
those growing in vivo. Quantitative analysis...
To evaluate the role of exogenous application of a phytochelating agent glutathione in increasing resistance against different heavy metals stress, nodal explants excised from 28-day-old in vitro seedlings of Spilanthes calva L. were cultured on Murashige and Skoog’s medium supplemented with 10 μM benzyl adenine and five different concentrations (1...
The present investigation was carried out to evaluate the level of salt toxicity on four novel chickpea genotypes (BG 372, BG 1105, BG 2019 and BG 2024) under in vitro condition for percentage seed germination and morphogenesis. All the four genotypes were exposed to different concentrations (0, 1, 50, 100 and 150 mM) of NaCl in the Murashige & Sko...
Micropropagation of Vernonia anthelmintica, an important medicinal and under exploited oilyielding herb of asteraceae has been achieved through seedling cotyledonary node explants. The explants if reared on Murashige and Skoog's (MS) medium supplemented with 0.1, 1, 5 and 10 %M levels of cytokinins, viz. N 6-benzyladenine (BA), kinetin (Kn), N 6-(2...
An efficient in vitro propagation system for an elite clone EC-353508 of Artemisia annua L., an important antimalarial drug plant has been developed. The best organogenic response, including adventitious multiple shoots and their elongation, was obtained when nodal explants were cultured on MS medium supplemented with 10μM 2-isopentenyl adenine (2i...
Three cultivars of tomato Pusa Ruby, Arka Vikas and Pusa Early Dwarf were subjected to osmotic stresses induced by mannitol,
polyethylene glycol and NaCl in vitro. Polypeptide patterns were analyzed during each stress treatment to differentiate between tolerant and sensitive cultivars.
The stresses induced more stress proteins in cv. Pusa Ruby comp...
Micropropagation has been achieved employing nodal explants excised from long term cultured shoots of an elite clone of Artimisia annua L. on MS medium supplemented with different cytokinins such as 2iP, BA or Kn individually. Of the various concentrations (1, 5, 7.5 and 10 M) tried, 10 M 2iP proved optimum for eliciting cent percent morphogenic re...
Psoralen, an important furanocoumarin occurring abundantly in seeds of Psoralea corylifolia is used as an anticancerous compound against leukemia and other cancer cell lines. Evaluation and isolation of psoralen from the calluses derived from different plant parts, viz. cotyledons, nodes, leaves and roots have been done in the present case for the...
Spilanthes acmella (Family: Asteraceae) commonly known as “toothache plant” is known to possess strong insecticidal and larvicidal properties. Experiments have been conducted to isolate and characterise the biolarvicidal compounds from the flower head extract of micropropagated S. acmella plants employing various tools like FT-IR, TLC, CC, NMR. FT-...