Vasudeo Zambare

Vasudeo Zambare
Sandip University · Life Science

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72
Publications
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Publications

Publications (72)
Article
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Lichens produce a large variety of secondary metabolites with diverse bioactivities, chemical structures, and physicochemical properties. For this reason, there is a growing interest in the use of lichen-derived bioactive molecules for drug discovery and development. Here, we report on the isolation, identification, and cytotoxic evaluation of gyro...
Article
Full-text available
Biodiesel is a promising renewable energy source that can be used together with other biofuels to help meet the growing energy needs of the rapidly increasing global population in an environmentally friendly way. In search for new and more efficient biodiesel production methods, this work reports on the synthesis and use of a novel biocatalyst that...
Article
Full-text available
Biodiesel is a biodegradable, renewable, and carbon-neutral alternative to petroleum diesel that can contribute to the global effort of minimizing the use of fossil fuels and meeting the ever-growing energy demands and stringent environmental constraints. The aim of this work was to 1) review the recent progress in feedstock development, including...
Article
The objective of this work was to examine opportunities for reducing the overall cost of lignocellulose hydrolysis to fermentable sugars. Primary sludge (PS), a negative cost lignocellulosic feedstock, was hydrolyzed sludge using a commercial cellulase preparation (Cellic® CTec2) in presence of non-ionic surfactants. Polyethylene glycol (PEG) 4000...
Article
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After a century, the whole world fighting against the pandemic viral infection: a novel coronavirus, COVID-19. Currently, more than 210 countries are suffering from COVID-19 with the number of affected countries and patients are exponentially increasing day by day. It became a global health issue where more than 2.7 million cases were reported with...
Article
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Introduction: To date, over 1,000 lichen secondary metabolites have been identified. Despite their promising cytotoxic properties, the number of literature reports on anticancer evaluation of lichenochemicals is limited. As cancer prevalence among the human population increases, there is growing interest in lichens as a natural source of secondary...
Article
BACKGROUNDA thermophilic lipase-producing Geobacillus thermodenitrificans strain AV-5 was isolated from the Mushroom Spring of Yellowstone National Park in WY, USA and studied as a source of lipase for transesterification of vegetable oils to biodiesel.RESULTSA maximum activity of 330 U/ml was produced on 2% (v/v) waste cooking oil at 50 °C, pH 8,...
Article
Full-text available
The maximum protease activity was obtained from P. aeruginosa MCM B-327 with soybean meal 1%, tryptone 1%, initial medium pH 7, agitation rate 250 rpm, aeration rate 0.75 vvm and fermentation temperature 30 °C, under submerged fermentation conditions (SmF). The protease productivity at 10 and 120L fermenters was found to be 16,021 and 9,975 UL-1h-...
Article
The chemical-catalyzed transesterification of vegetable oils to biodiesel has been industrially adopted due to its high conversion rates and low production time. However, this process suffers from several inherent drawbacks related to energy-intensive and environmentally unfriendly processing steps such as catalyst and product recovery, and waste w...
Article
Full-text available
MCM B-327 with soybean meal 1%, tryptone 1%, initial medium pH 7, agitation rate 250 rpm, aeration rate 0.75 vvm and fermentation temperature 30 °C, under submerged fermentation conditions (SmF). The protease productivity at 10 and 120L fermenters was found to be 16,021 and 9,975 UL-1h-1respectively. Kinetics of cell growth revealed that specific c...
Article
Full-text available
The optimization for poly-b-hydroxyalkanoate production was carried out with nutrient removal efficiency for total organic carbon (TOC), phosphate, and nitrate from palm oil mill effluent waste. The experiment was conducted in a fabricated fed-batch reactor and the data obtained was analyzed using central composite rotatable design and factorial de...
Book
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Features Covers almost all current and emerging omics applications in one volume Explores omics in the biomedical, environmental, and agricultural fields Discusses epigenomics, pharmacogenomics glycomics, spliceomics, metagenomics, toxicogenomics, environomics, and other cutting-edge areas Draws on the work of active, expert researchers around the...
Article
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How to cite this article: Zambare et al. (2013) IIOAB Letters, 3: Abstract Response surface methodological (RSM) optimization of protease by Pseudomonas aeruginosa MCM B327, increased 1.3-fold activity with 1% inoculum having cell density of 27.57 x 10 9 cells mL -1 at pH 7, 30 0 C and 72 h of incubation. Protease enzyme recovered from P. aeruginos...
Chapter
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One of the most important advances in biology has been our capacity to sequence the DNA of organisms. However, long after the conclusion of human genome sequencing, there are still regions of the genome that are unworkable; that is, they are difficult to mount and remain incomplete. Answers may come from second-generation sequencing, which has prod...
Article
Full-text available
Most of the plant protection strategies are focused on selection and application of the natural proteinase inhibitors (PIs) against insect pests. In addition, PIs also play a vital role in medicine for treatment of immunity related diseases. PI activity exists mainly in seeds, leaves and flowers of plants. In search of novel PIs, 135 different plan...
Article
Full-text available
India is the fifth largest primary energy consumer and fourth largest petroleum consumer after USA, China, and Japan. Despite the global economic crisis, India's economy is expected to grow at 6 to 8 %/year. There is an extreme dependence on petroleum products with considerable risks and environmental issues. Petroleum-derived transport fuels are o...
Article
Full-text available
Corynebacterium pseudotuberculosis is a pathogen of great veterinary and economic importance, since it affects livestock, mainly sheep and goats, worldwide, together with reports of its presence in camels in several Arabic, Asiatic, and East and West African countries, as well as Australia. In this article, we report the genome sequence of Coryneba...
Article
Full-text available
High PHA production and storage using palm oil mill effluent (POME) was investigated using a laboratory batch Bio-PORec® system under aerobic-feeding conditions. Results showed that maximum PHA was obtained at a specific rate (q(p)) of 0.343C-mol/C-molh when air was supplied at 20ml/min. The PHA yield was found to be 0.80C-mol/C-mol acetic acid (HA...
Article
Prairie cordgrass (PCG), Spartina pectinata, is considered an energy crop with potential for bioethanol production in North America. The focus of this study was to optimize enzymatic hydrolysis of PCG at higher solids loadings using a thermostable cellulase of a mutant Penicillium pinophilum ATCC 200401. A three variable, five-level central composi...
Article
Lichens are composite organisms consisting of a symbiotic association of a fungus (the mycobiont) with a photosynthetic partner (the phytobiont), usually either a green alga or cyanobacterium. The morphology, physiology and biochemistry of lichens are very different from those of the isolated fungus and alga in culture. Lichens occur in some of the...
Article
Response surface methodology (RSM) was used to optimize the enzymatic hydrolysis of corn stover (CS), an abundant agricultural residue in the USA. A five-level, three-variable central composite design (CCD) was employed in a total of 20 experiments to model and evaluate the impact of pH (4.1-6.0), solids loadings (6.6-23.4%), and enzyme loadings (6...
Article
Full-text available
Prokaryotes commonly present outer cell wall structures composed of a crystalline array of proteinaceous subunits, known as surface layers (S-layers). The ORF encoding the S-layer protein (SlpA) of Lactobacillus brevis was cloned into Lactococcus lactis under the transcriptional control of the xylose-inducible expression system (XIES). SlpA was sec...
Article
Full-text available
In this work, we report the whole-genome sequence of Corynebacterium pseudotuberculosis bv. equi strain CIP 52.97 (Collection Institut Pasteur), isolated in 1952 from a case of ulcerative lymphangitis in a Kenyan horse, which has evidently caused significant losses to agribusiness. Therefore, obtaining this genome will allow the detection of import...
Article
Full-text available
In this work, we report the complete genome sequence of a Corynebacterium pseudotuberculosis PAT10 isolate, collected from a lung abscess in an Argentine sheep in Patagonia, whose pathogen also required an investigation of its pathogenesis. Thus, the analysis of the genome sequence offers a means to better understanding of the molecular and genetic...
Conference Paper
Switchgrass (Panicum virgatum), a perennial grass native to North America, is a promising energy crop for bioethanol production. The aim of this study was to optimize the enzymatic saccharification of thermo-mechanically pretreated switchgrass using a thermostable cellulase from Geobacillus sp. in a three-level, four-variable central composite desi...
Conference Paper
Lipases (EC 3.1.1.3) are hydrolytic enzymes with a broad use in biocatalysis due to their versatility for industrial applications, their thermostability, relatively low cost and need for added cofactors. In the last two decades, lipases have gained interest because of their potential use among others in the food, detergent, and biodiesel industries...
Conference Paper
The Black Hills of South Dakota offer a biodiversity in lichen species with a great potential for biopharmaceutical applications. The present study was carried out to evaluate the antibacterial and antioxidant activities of the lichen isolate Xanthoparmelia somloensis, native to the Black Hills. The antibacterial activity was assayed against four c...
Conference Paper
Cellulases are a group of hydrolytic enzymes capable of hydrolyzing the most abundant organic polymer on earth – cellulose. These enzymes have enormous potential for commercial use in the food, beverages, textile, detergent and biofuels industries. Microorganisms have the ability to produce cellulases which act on the β-1-4 bonds to break down cell...
Article
A recently discovered thermophilic isolate, Geobacillus sp. R7, was shown to produce a thermostable cellulase with a high hydrolytic potential when grown on extrusion-pretreated agricultural residues such corn stover and prairie cord grass. At 70°C and 15-20% solids, the thermostable cellulase was able to partially liquefy solid biomass only after...
Article
Full-text available
The Institute of Integrative Omics and Applied Biotechnology (IIOAB), India (www.iioab.webs.com / www.iioab.ac.in) is a non-profit scientific organization that aims to provide a global platform for multidisciplinary research and advocacy. Other aspects of the organization are promotion of higher education, R&D, and to serve science, society, and th...
Article
Protease production from. aeruginosa MCM B-327, isolated from vermicompost pit soil, was achieved by using different agro-origin substrates. Maximum protease production was observed with wheat bran- tryptone followed by bengal gram flour - soya flour. The crude enzyme (3.35U) showed effective degelatinization of waste photographic films in tap wate...
Article
Full-text available
Switchgrass (Panicum virgatum), a perennial grass native to North America, is a promising energy crop for bioethanol production. The aim of this study was to optimize the enzymatic saccharification of thermo-mechanically pretreated switchgrass using a thermostable cellulase from Geobacillus sp. in a three-level, four-variable central composite desi...
Article
Production of amylase under submerged fermentation Bacillus sp. was investigated using wheat bran, soybean meal and CaCO 3 (WSC) medium. Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e., pH (11.35), temperature (35.16°C) and inoculum size (2.95%) on amylase production by applying a full factorial centra...
Article
Full-text available
Isolation and identification of dye decolourizing fungal isolates from textile dye effluent was carried out. The isolates of Aspergillus flavus, Aspergillus niger, Helminthosporium sp., Mucor sp. and Penicillium sp. were isolated from the textile effluent samples collected from Elampillai Jalagandapur. Different parameters were used for optimizing....
Article
Full-text available
Problem statement: Lipase is one of the important enzymes in food, pharmaceutical, detergent and biofuels industries. Search for the lipase with distinct features, possibly from germinating seeds, is of interest for industrial applications. Approach: The lipase produced by soybean oil seeds was partially purified and characterized in terms of the o...
Article
Full-text available
The dye decolorizing isolates, Bacillus sp., Klebsiella sp. Salmonella sp. and Pseudomonas sp. were isolated from the textile effluent samples collected from Elampillai, Tamil Nadu. Different parameters such as various carbon source, nitrogen source, temperature, pH and inoculum size were optimized for decolorization of Orange 3R by using bacterial...
Article
Full-text available
A thermophilic microbial consortium (TMC) producing hydrolytic (cellulolytic and xylanolytic) enzymes was isolated from yard waste compost following enrichment with carboxymethyl cellulose and birchwood xylan. When grown on 5% lignocellulosic substrates (corn stover and prairie cord grass) at 600C, the thermophilic consortium produced more xylanase...
Conference Paper
The literature suggests that current strategies for sustainable and economical processing of lignocellulose into biofuels is limited due to high production costs of lignocellulose-hydrolyzing enzymes; the very slow enzymatic hydrolysis kinetics of release of sugars from lignocellulose; and the low yields of sugars from lignocellulose. These issues...
Conference Paper
The production, characterization and application of a hydrolytic enzyme complex from a thermophilic microbial consortium (TMC) for efficient degradation of lignocellulosic substrates was examined. The production of carboxymethyl cellulase and xylanase was studies on pretreated prairie cord grass and corn stover. The TMC enzyme complex was active an...
Conference Paper
Full-text available
Among the bottlenecks in the development of a cost-effective process for cellulosic ethanol production are the slow rates of enzyme hydrolysis of lignocellulose and the relatively high enzyme production costs. From this perspective, the search for and the discovery of novel enzymes with unique properties and enhanced capabilities for cellulose degr...
Article
Full-text available
Laboratory scale experiments were carried out to test the efficiency of the extracellular protease from Bacillus cereus MCM B-326; cattle dung and commercial bate powder (ComBate) as bating agents on delimed buffalo hide. Protease treated pelt was free from scud and pigments, clean and fine grain, white, smooth and silkier with loosen fat. Histolog...
Article
The focus of this study was on production, purification and characterization of dehairing protease from Pseudomonas aeruginosa MCM B-327, isolated from vermicompost pit soil. Optimum protease activity, 395 U mL(-1), was observed in the medium containing soybean meal and tryptone, at pH 7 and 30 °C. The crude enzyme exhibited dehairing activity. As...
Article
Full-text available
The purpose of the present investigation is to enhance alkaline protease production by subjecting indigenous protease producing strain Trichoderma reesei MTCC-3929 to improvement by random mutagenesis by ultra-violet (UV) irradiation and N-Methyl-N’-nitro-N-nitroso guanidine (NTG) treatment. Mutants were screened as protease producers on the basis...
Article
Full-text available
Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconu...
Article
A neutral protease was purified from Bacillus sp. to electrophoretic homogeneity by using ammonium sulphate precipitation and 2-step-column chromatography. The purified protease expressed its maximum activity 40 o C and pH value of 7. It was stable up to 10-40 o C for 30 min of incubation and retained 80 and 65% of its activity at 50 and 60 o C, re...
Article
Full-text available
Laboratory scale experiments were carried out to test the efficiency of the extracellular protease from Bacillus cereus MCM B-326; cattle dung and commercial bate powder (ComBate) as bating agents on delimed buffalo hide. Protease treated pelt was free from scud and pigments, clean and fine grain, white, smooth and silkier with loosen fat. Histolog...
Article
Full-text available
A neutral protease, isolated from Bacillus sp. 158 was used for removing protein deposits from contact lenses. Partial purification of the protease was carried out using ammonium sulphate and factors affecting the enzyme activity, such as assay temperature and assay pH were characterized. The optimum pH and temperature for protease were found to be...
Article
Urinary tract infection (UTI) represents one of the most common diseases encountered in medical practice today and occurring from the neonate to the geriatric age group. Despite the widespread availability of antibiotics, it remains the most common bacterial infection in the human being. Four s pathogenic bacteria as Escherichia coli, Proteus sp Sh...
Article
The antibacterial activity of aqueous, ethanolic, methanolic, ether, acetone, chloroform and hexane extracts from Enicostema littorale plant has been evaluated, in vitro, against Serrasia sp:, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. All extracts of Enicostema littorale exhibited highest antibacterial activity against Sar...
Article
Full-text available
The present investigation describes microbial production of an alkaline protease and its use in dehairing of buffalo hide. Bacillus cereus produced extracellular protease when grown on a medium containing starch, wheat bran and soya flour (SWS). The ammonium sulphate precipitated (ASP) enzyme was applied for dehairing of buffalo hide. Microscopic o...
Article
Bacillus cereus MCM B-326, isolated from buffalo hide, produced an extracellular protease. Maximum protease production occurred (126.87+/-1.32 U ml(-1)) in starch soybean meal medium of pH 9.0, at 30 degrees C, under shake culture condition, with 2.8 x 10(8) cells ml(-1) as initial inoculum density, at 36 h. Ammonium sulphate precipitate of the enz...

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