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Introduction
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January 2003 - present
Publications
Publications (278)
The chemical composition of stingless bee honey and propolis depends on the plant sources they are derived from, and thus reflects the flora available in the vicinity of the hives, the preferences of the bee species, and the climate (altitude and temperature). To understand the relative influence of these factors, we studied the composition of hone...
The chemical investigation of Homotrigona apicalis propolis collected in Binh Dinh province, Vietnam, led to the isolation of nine compounds, including four sesquiterpenes: spathulenol (1), 1αH,5βH-aromandendrane-4β,10α-diol (2), 1β,6α-dihydroxy-4(15)-eudesmene (3), and 1βH,5βH-aromandendrane-4α,10β-diol (4); three triterpenes: acetyl oleanolic aci...
The chemical composition of stingless bee honey and propolis depends on the plant sources they are derived from, and thus reflects the flora available in the vicinity of the hives, the preference of the bee species, and the climate (altitude and temperature). To understand the relative influence of these factors, we studied the composition of honey...
Extraction is a vital step in obtaining pure bioactive natural compounds for medical, scientific and commercial use. Recently, interest in extracting natural products for applications across the food, pharmaceutical, and cosmetic industries has grown rapidly, driving demand for newer, more efficient extraction methods. To develop our understanding...
The present study aimed to analyze and compare the chemical profile and antioxidant capacity of propolis from different bee species and different regions. The chemical profiles of propolis from six stingless bee species (Tetragonula iridipennis, T. laeviceps, Lepidotrigona terminata, L. ventralis, Lisotrigona carpenteri and Homotrigona apicalis) co...
Natural deep eutectic solvents (NADES) have been developed in order to replace traditional volatile and toxic organic solvents for the extraction of biologically active substances from natural sources. Recent research has shown that NADES are able to retain and even improve the antioxidant properties of the molecules dissolved in them. The aim of t...
Nowadays, the environmentally friendly approach to everyday life routines including body supplementation with pharma-, nutraceuticals and dietary supplements gains popularity. This trend is implemented in pharmaceutical as well as cosmetic and antiageing industries by adopting a newly developed green chemistry approach. Following this trend, a new...
Unlabelled:
Propolis is a resinous natural product produced by honeybees using beeswax and plant exudates. The chemical composition of propolis is highly complex, and varies with region and season. This inherent chemical variability presents several challenges to its standardisation and quality control. The present review was aimed at highlighting...
Propolis is a resinous beehive product with extraordinary bioactivity and chemical richness, linked with the botanical sources of the resin. The potential of this product keeps captivating the scientific community, conducting to continuous and growing research on plant sources, composition, or applications in agriculture, cosmetics, pharmacy, odont...
The chemical composition and antimicrobial activity of propolis from a semi-arid region of Morocco were investigated. Fifteen compounds, including triterpenoids (1, 2, 7–12), macrocyclic diterpenes of ingol type (3–6) and aromatic derivatives (13–15), were isolated by various chromatographic methods. Their structures were elucidated by a combinatio...
Honey is a highly concentrated sugar solution that is a rich source of many bioactive compounds including numerous minor constituents. Among them, polyphenols such as flavonoids and phenolic acids are the most important classes of pharmacologically active compounds. Together with other minor constituents they are also considered potential chemical...
Propolis (bee glue) is a sticky resinous product manufactured by honeybees. In this book, dedicated to plant resins, propolis has a very special place: It is the only material which comes not from plants but from animals, from bees. This chapter summarizes the present status of knowledge on propolis: the approaches to its chemical analysis, the dif...
This study evaluated the in vitro antineoplastic and antiviral potential and in vivo toxicity of twelve extracts with different polarity obtained from the herbaceous perennial plant Geum urbanum L. (Rosaceae). In vitro cytotoxicity was determined by ISO 10993-5/2009 on bladder cancer, (T-24 and BC-3C), liver carcinoma (HEP-G2) and normal embryonic...
Objective
The mango tree Mangifera indica is known as one of the botanical sources of propolis in Tropical regions. There are two different materials which bees can collect from a mango tree to produce propolis: the resin of the tree bark, and the latex found on the fruits. We performed the study of the chemical profile of mango resin in comparison...
Burkitt’s lymphoma is a highly aggressive type of non-Hodgkin’s lymphoma, linked to the Epstein-Barr virus, which induces oxidative stress and DNA damage in the infected cells. We investigated the cytotoxicity and redox-modulating ability of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts from Geum urbanum L. roots and aerial parts on Burkitt...
Propolis (bee glue) is a sticky resinous product manufactured by honeybees. In this book, dedicated to plant resins, propolis has a very special place: It is the only material which comes not from plants but from animals, from bees. This chapter summarizes the present status of knowledge on propolis: the approaches to its chemical analysis, the dif...
Objective The mango tree Mangifera indica is known as one of the botanical sources of propolis in Tropical regions. There are two different materials which bees can collect from a mango tree to produce propolis: the resin of the tree bark, and the latex found on the fruits. We performed the study of the chemical profile of mango resin in comparison...
Recently, the honey and propolis of stingless bees have been attracting growing attention because of their health-promoting properties. However, studies on these products of African Meliponini are still very scarce. In this preliminary study, we analyzed the chemical composition of honey, two cerumen, and two resin deposits (propolis) samples of Me...
Propolis (bee glue) is a sticky material collected by bees from plant resins, it has been proven to possess numerous beneficial pharmacological properties: antibacterial, antiviral, antioxidant, antiinflammatory, immunostimulating, hepatoprotective, cytotoxic, etc. Nowadays, propolis is a popular remedy all over the world, and is available in eithe...
Geum urbanum L. is a medicinal plant used from ancient time against gastro-intestinal disorders, bleeding and inflammation of mucous membranes, gums (gingivitis), etc. In our previous works, we demonstrated the antibacterial and antioxidant properties of ethyl acetate (EtOAc) extracts in vitro. The aim of this study was to evaluate the possibility...
Geum urbanum L. (genus Geum, family Rosaceae) is a herbaceous
perennial plant species, commonly known as wood avens or St. Benedict’s herb
and widespread in temperate regions. It has been used since ancient times in the
European and particularly in the Bulgarian traditional medicine for treating gastrointestinal diseases as well as for reducing ble...
Geum urbanum L. is a medicinal plant used from ancient time against gastro-intestinal disorders, bleeding and inflammation of mucous membranes, gums (gingivitis), etc. In our previous works, we demonstrated the antibacterial and antioxidant properties of ethyl acetate (EtOAc) extracts in vitro. The aim of this study was to evaluate the possibility...
Burkitt's lymphoma is a highly aggressive type of non-Hodgkin's lymphoma, linked to the Epstein-Barr virus, which induces oxidative stress and DNA damage in the infected cells. We investigated the cytotoxicity and redox-modulating ability of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts from Geum urbanum L. roots and aerial parts on Burkitt...
Natural deep eutectic solvents (NADES) are a new alternative to toxic organic solvents. Their constituents are primary metabolites, non-toxic, biocompatible and sustainable. In this study four selected NADES were applied for the extraction of two medicinal plants: Sideritis scardica, and Plantago major as an alternative to water-alcohol mixtures, a...
A new dihydrochromene derivative, named lisofurvin (1) and a xanthone, named dihydrobrasixanthone B (2) together with twenty one known compounds (3−23) were isolated from propolis of the stingless bee Lisotrigona furva. Their chemical structures were determined by means of spectroscopic methods including 1D and 2D NMR, and MS. The chemical constitu...
Bulgaria and North Macedonia have a long history of the production and use of honey; however, there is an obvious lack of systematic and in-depth research on honey from both countries. The oak honeydew honey is of particular interest, as it is highly valued by consumers because of its health benefits. The aim of this study was to characterize honey...
Plants from the Veronica genus are used across the world as traditional remedies. In the present study, extracts from the aerial part of the scarcely investigated Veronica austriaca L., collected from two habitats in Bulgaria-the Balkan Mountains (Vau-1) and the Rhodopi Mountains (Vau-2), were analyzed by nuclear magnetic resonance (NMR) spectrosco...
Background
Pollen and propolis are two bee products with highly health promoting properties. But there are some limitations of raw propolis usage not only in daily consumption but also in putting it in food formulations. Propolis should be extracted to convert it into consumable form and ethanol is the first choice as a solvent. But ethanol consump...
Propolis is used as a constituent in over-the-counter preparations, food additives, cosmetics, etc. Bulgarian and Macedonian propolis samples were analyzed by gas chromatography-mass spectrometry (GC-MS) of ethanol extracts after silylation, in order to determine the presence of bioactive substances with antimicrobial and antioxidant activity and t...
Propolis is a bee product with various biological activities and phytochemical compositions. This study was aimed to
identify the chemical composition and botanical origin of Indonesian stingless bees propolis produced by Tetragonula
laeviceps in Banten and Heterotrigona itama in South Kalimantan, respectively. Propolis extracts were prepared usi...
This study compared the capacity of propolis extract (PE) and thyme waste extract (TWE) to prevent the oxidation of oil in water (O/W) emulsion, as well as their impact on emulsion apparent viscosity (AV) in the presence of wheat germ and almond oils as lipid phase. For this, central composite design (CCD) and principal component analysis (PCA) wer...
According to the World Health Organization the increasing antimicrobial resistance (AMR) to antibiotics is a global health problem. Quorum sensing (QS) is a cell density-dependent regulatory system, which is responsible for the drug resistance, pathogenicity, biofilm formation, etc. The aim of our study was to look for new QS-related phenotypes inh...
Background
Stingless bees (Meliponini), like honeybees Apis mellifera, collect plant resins in order to produce propolis (cerumen, geopropolis). This type of propolis has long been used in traditional medicine in Mexico, Brazil, Argentina, India, and Vietnam, as a remedy for improving health and treating various diseases. The scientific and commerc...
Finding new green solvents for extraction of bioactive natural compounds is among the first areas of research in green chemistry. Recently, a new kind of "green" solvents, the natural deep eutectic solvents (NADESs), was developed. In a particular combination and ratio natural compounds in solid state (primary metabolites) may generate a eutectic m...
The n-hexane extract of Knema pachycarpa fruits (Myristicaceae family), exhibiting strong anti-acetylcholinesterase activity, was investigated by gas chromatography/mass spectrometry and then purified by column chromatography. Guided by GC/MS profiling and bioassay, chromatographic separations led to the isolation of five new compounds: two anacard...
DEAR COLLEAGUE,
LET’S TOGETHER DEVELOP FURTHER DISCUSSION OF HONEY AS ZOOM CONFERENCE
In view of the growing interest to the treatment properties of HONEY AND BEE PRODUCTS (propolis, bee bread, pollen, royal jelly etc.), with especial focus on antiviral properties, as well as enormous success of our fruitful discussion at Research Gate https://bit...
The present study reports a promising antioxidant protection by a recently developed micellar propolis formulation, against oxidative stress in in vitro and in vivo models of toxicity. The formulation, based on poplar propolis encapsulated in poly(ethylene oxide)–b-poly(propylene oxide)–b-poly(ethylene oxide) triblock copolymer (PEO26–PPO40–PEO26)...
Propolis produced by the stingless bee Lisotrigona cacciae was studied for the first time. Using different chromatographic procedures, a total of eighteen constituents (phenols and triterpenes) were isolated, among which flavane 1, homoisoflavanes 2–4, and xanthones 5 and 6 were new for propolis. Propolis extract was also characterized by gas chrom...
1H NMR spectrum of a mixture 12 in CDCl3.
(PDF)
Flow chart of the sample analysis.
(PDF)
1H, HSQC and HMBC NMR spectra of compound 2 in CD3OD.
(PDF)
1H, HSQC, HMBC and NOESY NMR spectra of compound 3 in CD3OD.
(PDF)
1H and HSQC NMR spectra of compound 4 in DMSO-d6.
(PDF)
1H NMR spectrum of a mixture of 5 and 6 in CD3OD.
(PDF)
1H, 13C, HSQC and HMBC NMR spectra of compound 7 in CDCl3.
(PDF)
1H NMR spectrum of compound 10 in CDCl3.
(PDF)
1H NMR spectrum of compound 11 in CDCl3.
(PDF)
1H NMR spectrum of compound 8 in CD3OD.
(PDF)
1H NMR spectrum of compound 9 in CD3OD.
(PDF)
1H, 13C, DEPT 135, HSQC, HMBC and NOESY NMR spectra of compound 1 in CD3OD.
(PDF)
Caffeic acid phenethyl ester (CAPE), a hydrophobic constituent of poplar propolis of valuable biological activity, was immobilized in poly(ethylene oxide)-b-poly(e-caprolactone)-b-poly(ethyl-ene oxide) (PEO-b-PCL-b-PEO) copolymer micelles to improve its solubility in water. CAPE was loaded in the micelles by dialysis, achieving ca. 50% encapsulatio...
Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior
with metastatic progression. Because propolis potential for the development of
new antitumoral drugs has been indicated, we evaluated the chemical composition
of Colombian propolis samples and the mechanisms involved in their cytotoxic effects
on OSA cells. The ch...
This study was performed to evaluate the total phenols, flavonoids, and antioxidant activities of twenty-four propolis samples from different regions of Morocco. In addition, two samples were screened regarding the antibacterial effect against four Staphylococcus aureus strains. Gas chromatography coupled to mass spectra (GC-MS) analysis was done f...
Table S1: chemical composition of hydro-alcoholic extract of Moroccan propolis.
Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The ch...
Caffeic acid phenethyl ester is the most studied of all the individual constituents of propolis. It possesses diverse and potent pharmacological activities: anticancer, antibacterial, antiviral, anti-inflammatory, immunomodulating, cytotoxic, neuroprotective, hepatoprotective, cardioprotective, etc. The interest in the versatile pharmacological act...
Honeybees rely on plants for everything they need to keep the colony running; plant nectar and pollen are their only carbohydrate and protein food sources. By foraging to satisfy their basic nutritional demand, honeybees inevitably gather specialized plant metabolites as part of the nectar and pollen. In general, these compounds possess biological...
Genus Thymus comprises more than 250 species of perennial herbaceous or fruticose plants, classified into 8 sections. Total 66 species with numerous subspecies and varieties are listed in Flora Europaea. Twenty species occur naturally in Bulgaria. One of them is endemic to the country (Thymus perinicus), four are endemics to the Balkan Peninsula (T...
Propolis (bee glue) is a resinous hive product collected by bees from certain plant sources and known since ancient times for its healing properties. There is a number of scientific evidence that it has antimicrobial, antioxidant, antiinflammatory, immunomodulating, antiulcer, antidiabetic and anticancer activities. Because of its broad spectrum of...
Dichloromethane extract of propolis (DCME) originating from Pitcairn Island demonstrated potent cytotoxicity against triple-negative MDA-MB-231 human breast carcinoma cells. The results from MTT assay showed that DCME inhibits the growth of the cancer cells in a dose- and time-dependent manner and upon the cell growth inhibition propolis extract pr...
Propolis is known to possess antioxidant activity. However, there is no information on this activity in emulsions O/W. The protective effect of propolis on the oxidation and rheological properties of emulsions O/W containing wheat germ and almond oils was evaluated. Emulsions O/W were prepared with different concentration of propolis extract, almon...
The Verbascum species are widely used traditional herb remedies against respiratory, inflammatory conditions and disorders. In the present study methanol extract of the aerial parts of the endemic Verbascum nobile Velen, was investigated and two novel iridoid glycosides 1 and 2, together with nine known constituents: iridoids, phenylethanoids, and...
It is reported for first time, that silver modified nanoporous MCM-41 or SBA-15 silicas are promising carriers for the preparation of poplar propolis loaded dermal formulations. MCM-41 or SBA-15 were successfully modified with 5–15 nm sized silver nanoparticles by direct or post synthesis methods. The formed silver nanoparticles are stabilized in t...
Many Geum species are known to be rich in biologically active compounds and therefore could be a source of new natural products with pharmacological potential. The medicinal plant Geum urbanum L. is widespread in Bulgaria and has been used in folk medicine. In the present study, the methanol extracts of the roots and aerial parts of G. urbanum and...
Propolis (bee glue) is a resinous hive product collected by honeybees from certain plant sources. lt has a long history of being used in traditional medicine and nowadays it is extensively used in food to improve health and prevent diseases such as inflammation, heart disease, diabetes, and even cancer. Because of its broad spectrum of biological a...
We report on the efficient loading of poplar propolis into core-shell micelles based on amphiphilic poly(ethylene oxide)-block-poly(n-butyl acrylate) (PEO-b-PnBA) diblock copolymers. The copolymers comprising a hydrophilic PEO block of constant length (113 monomer units) and a soft hydrophobic PnBA block of three different lengths (53, 99 and157 mo...
In this study, we assessed the therapeutic potential of propolis from Poland and performed chemical analysis by GC–MS, as well as determined its botanical origin. Chemical constituents typical for bud exudates of Populus nigra (section Aigeiros) were determined, however, glycerol esters of phenolic acids, as well as unusually high amounts of p-coum...
In Bulgaria, propolis tincture is among of the most popular home-made remedies. Bulgarian propolis has been studied and it was found to originate from the bud resin of the black poplar Populus nigra L.; the chemical constituents responsible for its biological activity are flavonoid aglycones (flavones, flavonols, flavanones, dihydroflavonols), subs...
The aim of this study was to examine the antimicrobial properties and chemical composition of Thai Apis mellifera propolis from different locations. All propolis samples demonstrated significant activity (Minimal Inhibitory Concentration < 1000 µg/mL) against Gram-positive bacteria Bacillus cereus, Staphylococcus aureus, S. epidermidis and methicil...
The objective of the present study was to characterize chemically the essential oils of two distinct propolis types: Brazilian red and Taiwanese green. Unlike the non-volatile chemical composition of these types of propolis, which has been extensively studied, the knowledge of the essential oils is scarce or even not
investigated. The essential oil...
The extracts of some propolis samples were analysed by GC-MS and then purified by column chromatography. The latter led to the isolation of a new mono-ether of glycerol, 1'-O-eicosanyl glycerol and a new triterpene, methyl-3β,27-dihydroxycycloart-24-en-26-oate together with known triterpenoids namely betulin, 3β-hydroxylanostan-9,24-dien-21-oic aci...
