Vassya Bankova

Vassya Bankova
Bulgarian Academy of Sciences | BAS · Institute of Organic Chemistry with Center for Phytochemistry

PhD, DSc

About

269
Publications
73,281
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15,832
Citations
Additional affiliations
January 2003 - present
Bulgarian Academy of Sciences
Position
  • Professor (Full)

Publications

Publications (269)
Article
Full-text available
Natural deep eutectic solvents (NADES) have been developed in order to replace traditional volatile and toxic organic solvents for the extraction of biologically active substances from natural sources. Recent research has shown that NADES are able to retain and even improve the antioxidant properties of the molecules dissolved in them. The aim of t...
Article
Full-text available
Nowadays, the environmentally friendly approach to everyday life routines including body supplementation with pharma-, nutraceuticals and dietary supplements gains popularity. This trend is implemented in pharmaceutical as well as cosmetic and antiageing industries by adopting a newly developed green chemistry approach. Following this trend, a new...
Article
Full-text available
Propolis is a resinous natural product produced by honeybees using beeswax and plant exudates. The chemical composition of propolis is highly complex, and varies with region and season. This inherent chemical variability presents several challenges to its standardisation and quality control. The present review was aimed at highlighting marker compo...
Article
Propolis is a resinous beehive product with extraordinary bioactivity and chemical richness, linked with the botanical sources of the resin. The potential of this product keeps captivating the scientific community, conducting to continuous and growing research on plant sources, composition, or applications in agriculture, cosmetics, pharmacy, odont...
Article
Full-text available
The chemical composition and antimicrobial activity of propolis from a semi-arid region of Morocco were investigated. Fifteen compounds, including triterpenoids (1, 2, 7–12), macrocyclic diterpenes of ingol type (3–6) and aromatic derivatives (13–15), were isolated by various chromatographic methods. Their structures were elucidated by a combinatio...
Article
Honey is a highly concentrated sugar solution that is a rich source of many bioactive compounds including numerous minor constituents. Among them, polyphenols such as flavonoids and phenolic acids are the most important classes of pharmacologically active compounds. Together with other minor constituents they are also considered potential chemical...
Article
Full-text available
This study evaluated the in vitro antineoplastic and antiviral potential and in vivo toxicity of twelve extracts with different polarity obtained from the herbaceous perennial plant Geum urbanum L. (Rosaceae). In vitro cytotoxicity was determined by ISO 10993-5/2009 on bladder cancer, (T-24 and BC-3C), liver carcinoma (HEP-G2) and normal embryonic...
Article
Full-text available
Objective The mango tree Mangifera indica is known as one of the botanical sources of propolis in Tropical regions. There are two different materials which bees can collect from a mango tree to produce propolis: the resin of the tree bark, and the latex found on the fruits. We performed the study of the chemical profile of mango resin in comparison...
Article
Full-text available
Burkitt’s lymphoma is a highly aggressive type of non-Hodgkin’s lymphoma, linked to the Epstein-Barr virus, which induces oxidative stress and DNA damage in the infected cells. We investigated the cytotoxicity and redox-modulating ability of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts from Geum urbanum L. roots and aerial parts on Burkitt...
Preprint
Full-text available
Objective The mango tree Mangifera indica is known as one of the botanical sources of propolis in Tropical regions. There are two different materials which bees can collect from a mango tree to produce propolis: the resin of the tree bark, and the latex found on the fruits. We performed the study of the chemical profile of mango resin in comparison...
Article
Full-text available
Recently, the honey and propolis of stingless bees have been attracting growing attention because of their health-promoting properties. However, studies on these products of African Meliponini are still very scarce. In this preliminary study, we analyzed the chemical composition of honey, two cerumen, and two resin deposits (propolis) samples of Me...
Article
Propolis (bee glue) is a sticky material collected by bees from plant resins, it has been proven to possess numerous beneficial pharmacological properties: antibacterial, antiviral, antioxidant, antiinflammatory, immunostimulating, hepatoprotective, cytotoxic, etc. Nowadays, propolis is a popular remedy all over the world, and is available in eithe...
Article
Full-text available
Geum urbanum L. is a medicinal plant used from ancient time against gastro-intestinal disorders, bleeding and inflammation of mucous membranes, gums (gingivitis), etc. In our previous works, we demonstrated the antibacterial and antioxidant properties of ethyl acetate (EtOAc) extracts in vitro. The aim of this study was to evaluate the possibility...
Chapter
Full-text available
Propolis (bee glue) is a sticky resinous product manufactured by honeybees. In this book, dedicated to plant resins, propolis has a very special place: It is the only material which comes not from plants but from animals, from bees. This chapter summarizes the present status of knowledge on propolis: the approaches to its chemical analysis, the dif...
Conference Paper
Full-text available
Geum urbanum L. (genus Geum, family Rosaceae) is a herbaceous perennial plant species, commonly known as wood avens or St. Benedict’s herb and widespread in temperate regions. It has been used since ancient times in the European and particularly in the Bulgarian traditional medicine for treating gastrointestinal diseases as well as for reducing ble...
Preprint
Full-text available
Geum urbanum L. is a medicinal plant used from ancient time against gastro-intestinal disorders, bleeding and inflammation of mucous membranes, gums (gingivitis), etc. In our previous works, we demonstrated the antibacterial and antioxidant properties of ethyl acetate (EtOAc) extracts in vitro. The aim of this study was to evaluate the possibility...
Preprint
Full-text available
Burkitt's lymphoma is a highly aggressive type of non-Hodgkin's lymphoma, linked to the Epstein-Barr virus, which induces oxidative stress and DNA damage in the infected cells. We investigated the cytotoxicity and redox-modulating ability of ethyl acetate (EtOAc) and n-butanol (n-BuOH) extracts from Geum urbanum L. roots and aerial parts on Burkitt...
Article
Full-text available
Natural deep eutectic solvents (NADES) are a new alternative to toxic organic solvents. Their constituents are primary metabolites, non-toxic, biocompatible and sustainable. In this study four selected NADES were applied for the extraction of two medicinal plants: Sideritis scardica, and Plantago major as an alternative to water-alcohol mixtures, a...
Article
A new dihydrochromene derivative, named lisofurvin (1) and a xanthone, named dihydrobrasixanthone B (2) together with twenty one known compounds (3−23) were isolated from propolis of the stingless bee Lisotrigona furva. Their chemical structures were determined by means of spectroscopic methods including 1D and 2D NMR, and MS. The chemical constitu...
Article
Full-text available
Bulgaria and North Macedonia have a long history of the production and use of honey; however, there is an obvious lack of systematic and in-depth research on honey from both countries. The oak honeydew honey is of particular interest, as it is highly valued by consumers because of its health benefits. The aim of this study was to characterize honey...
Article
Full-text available
Plants from the Veronica genus are used across the world as traditional remedies. In the present study, extracts from the aerial part of the scarcely investigated Veronica austriaca L., collected from two habitats in Bulgaria-the Balkan Mountains (Vau-1) and the Rhodopi Mountains (Vau-2), were analyzed by nuclear magnetic resonance (NMR) spectrosco...
Article
Background Pollen and propolis are two bee products with highly health promoting properties. But there are some limitations of raw propolis usage not only in daily consumption but also in putting it in food formulations. Propolis should be extracted to convert it into consumable form and ethanol is the first choice as a solvent. But ethanol consump...
Article
Full-text available
Propolis is used as a constituent in over-the-counter preparations, food additives, cosmetics, etc. Bulgarian and Macedonian propolis samples were analyzed by gas chromatography-mass spectrometry (GC-MS) of ethanol extracts after silylation, in order to determine the presence of bioactive substances with antimicrobial and antioxidant activity and t...
Article
Full-text available
Propolis is a bee product with various biological activities and phytochemical compositions. This study was aimed to identify the chemical composition and botanical origin of Indonesian stingless bees propolis produced by Tetragonula laeviceps in Banten and Heterotrigona itama in South Kalimantan, respectively. Propolis extracts were prepared usi...
Article
This study compared the capacity of propolis extract (PE) and thyme waste extract (TWE) to prevent the oxidation of oil in water (O/W) emulsion, as well as their impact on emulsion apparent viscosity (AV) in the presence of wheat germ and almond oils as lipid phase. For this, central composite design (CCD) and principal component analysis (PCA) wer...
Article
Full-text available
According to the World Health Organization the increasing antimicrobial resistance (AMR) to antibiotics is a global health problem. Quorum sensing (QS) is a cell density-dependent regulatory system, which is responsible for the drug resistance, pathogenicity, biofilm formation, etc. The aim of our study was to look for new QS-related phenotypes inh...
Article
Background Stingless bees (Meliponini), like honeybees Apis mellifera, collect plant resins in order to produce propolis (cerumen, geopropolis). This type of propolis has long been used in traditional medicine in Mexico, Brazil, Argentina, India, and Vietnam, as a remedy for improving health and treating various diseases. The scientific and commerc...
Article
Full-text available
Finding new green solvents for extraction of bioactive natural compounds is among the first areas of research in green chemistry. Recently, a new kind of "green" solvents, the natural deep eutectic solvents (NADESs), was developed. In a particular combination and ratio natural compounds in solid state (primary metabolites) may generate a eutectic m...
Article
Full-text available
The n-hexane extract of Knema pachycarpa fruits (Myristicaceae family), exhibiting strong anti-acetylcholinesterase activity, was investigated by gas chromatography/mass spectrometry and then purified by column chromatography. Guided by GC/MS profiling and bioassay, chromatographic separations led to the isolation of five new compounds: two anacard...
Presentation
Full-text available
DEAR COLLEAGUE, LET’S TOGETHER DEVELOP FURTHER DISCUSSION OF HONEY AS ZOOM CONFERENCE In view of the growing interest to the treatment properties of HONEY AND BEE PRODUCTS (propolis, bee bread, pollen, royal jelly etc.), with especial focus on antiviral properties, as well as enormous success of our fruitful discussion at Research Gate https://bit...
Article
Full-text available
The present study reports a promising antioxidant protection by a recently developed micellar propolis formulation, against oxidative stress in in vitro and in vivo models of toxicity. The formulation, based on poplar propolis encapsulated in poly(ethylene oxide)–b-poly(propylene oxide)–b-poly(ethylene oxide) triblock copolymer (PEO26–PPO40–PEO26)...
Article
Full-text available
Propolis produced by the stingless bee Lisotrigona cacciae was studied for the first time. Using different chromatographic procedures, a total of eighteen constituents (phenols and triterpenes) were isolated, among which flavane 1, homoisoflavanes 2–4, and xanthones 5 and 6 were new for propolis. Propolis extract was also characterized by gas chrom...
Data
1H NMR spectrum of a mixture 12 in CDCl3. (PDF)
Data
Flow chart of the sample analysis. (PDF)
Data
1H, HSQC and HMBC NMR spectra of compound 2 in CD3OD. (PDF)
Data
1H, HSQC, HMBC and NOESY NMR spectra of compound 3 in CD3OD. (PDF)
Data
1H and HSQC NMR spectra of compound 4 in DMSO-d6. (PDF)
Data
1H NMR spectrum of a mixture of 5 and 6 in CD3OD. (PDF)
Data
1H, 13C, HSQC and HMBC NMR spectra of compound 7 in CDCl3. (PDF)
Data
1H NMR spectrum of compound 10 in CDCl3. (PDF)
Data
1H NMR spectrum of compound 11 in CDCl3. (PDF)
Data
1H NMR spectrum of compound 8 in CD3OD. (PDF)
Data
1H NMR spectrum of compound 9 in CD3OD. (PDF)
Data
1H, 13C, DEPT 135, HSQC, HMBC and NOESY NMR spectra of compound 1 in CD3OD. (PDF)
Article
Full-text available
Caffeic acid phenethyl ester (CAPE), a hydrophobic constituent of poplar propolis of valuable biological activity, was immobilized in poly(ethylene oxide)-b-poly(e-caprolactone)-b-poly(ethyl-ene oxide) (PEO-b-PCL-b-PEO) copolymer micelles to improve its solubility in water. CAPE was loaded in the micelles by dialysis, achieving ca. 50% encapsulatio...
Preprint
Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The ch...
Article
Full-text available
This study was performed to evaluate the total phenols, flavonoids, and antioxidant activities of twenty-four propolis samples from different regions of Morocco. In addition, two samples were screened regarding the antibacterial effect against four Staphylococcus aureus strains. Gas chromatography coupled to mass spectra (GC-MS) analysis was done f...
Data
Table S1: chemical composition of hydro-alcoholic extract of Moroccan propolis.
Article
Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The ch...
Article
Full-text available
Caffeic acid phenethyl ester is the most studied of all the individual constituents of propolis. It possesses diverse and potent pharmacological activities: anticancer, antibacterial, antiviral, anti-inflammatory, immunomodulating, cytotoxic, neuroprotective, hepatoprotective, cardioprotective, etc. The interest in the versatile pharmacological act...
Article
Honeybees rely on plants for everything they need to keep the colony running; plant nectar and pollen are their only carbohydrate and protein food sources. By foraging to satisfy their basic nutritional demand, honeybees inevitably gather specialized plant metabolites as part of the nectar and pollen. In general, these compounds possess biological...
Poster
Full-text available
Genus Thymus comprises more than 250 species of perennial herbaceous or fruticose plants, classified into 8 sections. Total 66 species with numerous subspecies and varieties are listed in Flora Europaea. Twenty species occur naturally in Bulgaria. One of them is endemic to the country (Thymus perinicus), four are endemics to the Balkan Peninsula (T...
Poster
Propolis (bee glue) is a resinous hive product collected by bees from certain plant sources and known since ancient times for its healing properties. There is a number of scientific evidence that it has antimicrobial, antioxidant, antiinflammatory, immunomodulating, antiulcer, antidiabetic and anticancer activities. Because of its broad spectrum of...
Article
Dichloromethane extract of propolis (DCME) originating from Pitcairn Island demonstrated potent cytotoxicity against triple-negative MDA-MB-231 human breast carcinoma cells. The results from MTT assay showed that DCME inhibits the growth of the cancer cells in a dose- and time-dependent manner and upon the cell growth inhibition propolis extract pr...
Article
Full-text available
Propolis is known to possess antioxidant activity. However, there is no information on this activity in emulsions O/W. The protective effect of propolis on the oxidation and rheological properties of emulsions O/W containing wheat germ and almond oils was evaluated. Emulsions O/W were prepared with different concentration of propolis extract, almon...
Article
The Verbascum species are widely used traditional herb remedies against respiratory, inflammatory conditions and disorders. In the present study methanol extract of the aerial parts of the endemic Verbascum nobile Velen, was investigated and two novel iridoid glycosides 1 and 2, together with nine known constituents: iridoids, phenylethanoids, and...
Article
It is reported for first time, that silver modified nanoporous MCM-41 or SBA-15 silicas are promising carriers for the preparation of poplar propolis loaded dermal formulations. MCM-41 or SBA-15 were successfully modified with 5–15 nm sized silver nanoparticles by direct or post synthesis methods. The formed silver nanoparticles are stabilized in t...
Article
Full-text available
Many Geum species are known to be rich in biologically active compounds and therefore could be a source of new natural products with pharmacological potential. The medicinal plant Geum urbanum L. is widespread in Bulgaria and has been used in folk medicine. In the present study, the methanol extracts of the roots and aerial parts of G. urbanum and...
Poster
Propolis (bee glue) is a resinous hive product collected by honeybees from certain plant sources. lt has a long history of being used in traditional medicine and nowadays it is extensively used in food to improve health and prevent diseases such as inflammation, heart disease, diabetes, and even cancer. Because of its broad spectrum of biological a...
Article
We report on the efficient loading of poplar propolis into core-shell micelles based on amphiphilic poly(ethylene oxide)-block-poly(n-butyl acrylate) (PEO-b-PnBA) diblock copolymers. The copolymers comprising a hydrophilic PEO block of constant length (113 monomer units) and a soft hydrophobic PnBA block of three different lengths (53, 99 and157 mo...
Article
Full-text available
In this study, we assessed the therapeutic potential of propolis from Poland and performed chemical analysis by GC–MS, as well as determined its botanical origin. Chemical constituents typical for bud exudates of Populus nigra (section Aigeiros) were determined, however, glycerol esters of phenolic acids, as well as unusually high amounts of p-coum...
Article
Full-text available
In Bulgaria, propolis tincture is among of the most popular home-made remedies. Bulgarian propolis has been studied and it was found to originate from the bud resin of the black poplar Populus nigra L.; the chemical constituents responsible for its biological activity are flavonoid aglycones (flavones, flavonols, flavanones, dihydroflavonols), subs...
Article
The aim of this study was to examine the antimicrobial properties and chemical composition of Thai Apis mellifera propolis from different locations. All propolis samples demonstrated significant activity (Minimal Inhibitory Concentration < 1000 µg/mL) against Gram-positive bacteria Bacillus cereus, Staphylococcus aureus, S. epidermidis and methicil...
Article
Full-text available
The objective of the present study was to characterize chemically the essential oils of two distinct propolis types: Brazilian red and Taiwanese green. Unlike the non-volatile chemical composition of these types of propolis, which has been extensively studied, the knowledge of the essential oils is scarce or even not investigated. The essential oil...