Vasily Zlatogursky

Vasily Zlatogursky
Saint Petersburg State University | SPBU · Faculty of Biology

PhD

About

36
Publications
11,157
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1,203
Citations
Additional affiliations
January 2010 - present
Saint Petersburg State University
Position
  • Professor (Assistant)

Publications

Publications (36)
Article
Raphidocystis pallida, a centrohelid heliozoan with unusually shaped tubular siliceous scales, was reisolated from Jamor river, Portugal and studied with the use of light‐ and electron microscopy. In enriched cultures, the cells were naked, devoid of siliceous external skeleton with the exception of several scales present in one cell. Instead, such...
Article
Marophrys is a genus of spicules-bearing centrohelids belonging to Heterophrys-like organisms (HLO’s). Here Marophrys nikolaevi spec. nov. is described. Four strains were isolated from brackish waters (16–22 ppt) of the Tuzlukkol’ River, the Tuzluchnoe Lake (South Urals, Russia) and the Black Sea. All the strains were characterised with light micro...
Article
Full-text available
The endosymbiotic origin of plastids from cyanobacteria gave eukaryotes photosynthetic capabilities and launched the diversification of countless forms of algae. These primary plastids are found in members of the eukaryotic supergroup Archaeplastida. All known archaeplastids still retain some form of primary plastids, which are widely assumed to ha...
Article
The majority of centrohelids bear external coverings consisting of organic spicules or siliceous scales. Cyst coverings are usually reinforced with additional layers of modified scales. The cyst wall of Raphidiophrys heterophryoidea has unusual and complex structure. It consists of 3 different types of scales and includes the mosaic scale layer not...
Article
A new family, genus and species of centrohelid heliozoans, Clypifer cribrifer gen. nov., sp. nov. (Clypiferidae fam. nov.), from the Gulf of Aqaba (Israel) was studied with light and electron microscopy and SSU rRNA gene sequencing. Clypifer cribrifer has only one type of scales, partially running up the sides of the axopodia. Plate scales [0.8–2.3...
Article
Meringosphaera is an enigmatic marine protist without clear phylogenetic affiliation, but it has long been suggested to be a chrysophytes-related autotroph. Microscopy-based reports indicate that it has a worldwide distribution, but no sequence data exists so far. We obtained the first 18S rDNA sequence for M. mediterranea (identified using light a...
Preprint
Full-text available
The endosymbiotic origin of plastids from cyanobacteria gave eukaryotes photosynthetic capabilities and launched the diversification of countless forms of algae. These primary plastids are found in members of the eukaryotic supergroup Archaeplastida, and are widely assumed to have a single origin. Here, we used single-cell genomics from natural sam...
Article
A new strain of planktonic heliozoans (ZI172) belonging to the genus Microheliella (the sister group of Cryptista in Diaphoretickes), closely related to the only one known strain of Microheliella maris (CCAP 1945/1), was studied with light microscopy and SSU rRNA gene sequencing. Morphometric data obtained from 127 cells and based on 254 measuremen...
Preprint
Full-text available
Meringosphaera is an enigmatic marine protist without clear phylogenetic affiliation, but it has long been suggested to be a chrysophytes-related autotroph. Microscopy-based reports indicate that it has a worldwide distribution, but no sequence data exists so far. We obtained the first 18S rDNA sequence for M. mediterranea (identified using light a...
Article
Full-text available
While the formation of actin-based pseudopodia is presumably an ancestral eukaryotic feature, microtubule-based pseudopodia represent later modifications that were acquired independently in different lineages. Mapping morphological information onto molecular phylogenetic trees led to different scenarios of the microtubule-based outgrowths evolution...
Article
Full-text available
Background The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T. rubrum sensu stricto and T. violaceum , as well as other less well established or debatable taxa like T. soudanense , T. kuryangei and T. megninii . Our previous study provided limited evidence in favour of t...
Preprint
Full-text available
Background The Trichophyton rubrum species group consists of prevalent causative agents of human skin, nail and hair infections, including T. rubrum sensu stricto and T. violaceum , as well as other less well established or debatable taxa like T. soudanense , T. kuryangei and T. megninii . Our previous study provided limited evidence in favour of t...
Article
The life cycle of the centrohelid heliozoan Raphidiophrys heterophryoidea Zlatogursky, 2012 was studied with light and electron microscopy in clonal cultures from the type locality. The alternation of two types of trophozoites, having contrastingly different morphology, was observed. Type 1 trophozoites morphology matched the original description....
Article
The diversity of centrohelids in inland saline waters was studied with metabarcoding for the first time. The fragment of V6–V7 regions of 18S rDNA was sequenced with newly designed primers. Obtained OTUs were identified with molecular phylogenetic analysis and comparison of the signatures in 39es9 hairpin of V7. The obtained data included some OTUs...
Article
A new genus and species of centrohelid heliozoan Pinjata ruminata from the Tuzlukkol’ River (Orenburg Region of Russia) and Gorʼkoe Lake (Chelyabinsk Region of Russia) is studied with light‐ and electron microscopy. P. ruminata has two types of plate scales, partially running up the sides of the axopodia. Inner plate scales (3.2–4.9 × 1.5–2.6 μm) a...
Article
This revision of the classification of eukaryotes follows that of Adl et al., 2012[J. Euk. Microbiol.59(5)] and retains an emphasis on protists. Changes since have improved the resolution of many nodes in phylogenetic analyses. For some clades even families are being clearly resolved. As we had predicted,environmental sampling in the intervening ye...
Article
Full-text available
This revision of the classification of eukaryotes follows that of Adl et al., 2012 [J. Euk. Microbiol. 59(5)] and retains an emphasis on protists. Changes since have improved the resolution of many nodes in phylogenetic analyses. For some clades even families are being clearly resolved. As we had predicted, environmental sampling in the intervening...
Article
Two closely related new species of centrohelid heliozoans with unusual morphology were studied with light and electron microscopy. Sequences of the 18S rRNA gene were also obtained and secondary structure of 18S rRNA molecule reconstructed. The cells, covered with inner siliceous plate scales formed colonies. The entire colony was surrounded with a...
Article
Centrohelid heliozoan Raphidocystis glabra was reisolated for the first time after initial description and put into a clonal culture. Its correct identification was confirmed by scanning and transmission electron microscopy of scales. The first light microscopy data from the living cells were obtained. Phylogenetic analysis of its position using 18...
Article
The first application of DNA-barcoding for the centrohelids is reported. The character-rich genus Acanthocystis was chosen to compare sequence divergence and morphological similarity. Acanthocystis nichollsi, an easily identifiable and well outlined species, was isolated from four remote locations; A. costata; A. takahashii (2 strains) and A. turfa...
Article
A new species of centrohelid heliozoan Acanthocystis amura from the Amur River, the Far East of Russia was studied with light- and electron microscopy. Acanthocystis amura has simple oval plate scales (1.2–2.6 x 1.0–1.8 μm) with an axial thickening and a thin border as well as two types of spine scales. The spine scales of type 1 were 1.3–4.8 μm lo...
Article
Full-text available
Amoebae of the genus Korotnevella are covered with scales, the structure of which is believed to be species-specific and allows distinguishing species reliably at the morphological level. We studied members of this genus in order to assess the genetic structure of the local populations of amoebae. For the present study we isolated nine freshwater s...
Article
Belonocystis Rainer, 1968 is an enigmatic protist genus, which currently lacks any supergroup affiliation. The spherical cells of this organism move on the substratum using fine non-branching pseudopodia. The cell surface is surrounded with a spiky covering. Belonocystis marina sp. nov. was studied using light- and electron microscopy. It was clear...
Article
Full-text available
A new freshwater species of naked lobose amoebae Korotnevella venosa n. sp. isolated from freshwater pond in St. Petersburg, Russia was studied with light and transmission electron microscopy. Basket scales of this species have six vertical columns supporting perforated rim. The latter has tongue-like broadening with membranous region. Vertical col...
Article
Full-text available
Trichophyton spp. are important causative agents of superficial mycoses. The phylogeny of the genus and accurate strain identification, based on the ribosomal ITS region sequencing, are still under development. The present work is aimed at (i) inferring the genus phylogeny from partial ITS, LSU and BT2 sequences (ii) description of ribosomal ITS re...
Article
Full-text available
Assembling the global eukaryotic tree of life has long been a major effort of Biology. In recent years, pushed by the new availability of genome-scale data for microbial eukaryotes, it has become possible to revisitmany evolutionary enigmas. However, some of the most ancient nodes, which are essential for inferring a stable tree, have remained high...
Article
Centrohelid heliozoans are non-ciliary axopodial protists of uncertain affinities. Cell coverings (organic spicules, siliceous plate-scales and spine-scales) are the main character for their taxonomy. Phylogenetic reconstructions of this group are mostly based on the sequences of 18S rDNA. Here molecular and microscopical data on Raphidiophrys hete...
Article
Full-text available
ACTA PROTOZOOLOGICA Two New Species of Centrohelid Heliozoans: Acanthocystis costata sp. nov. and Choanocystis symna sp. nov. Abstract. Two new species of centrohelid heliozoans Acanthocystis costata and Choanocystis symna from a freshwater lake on Valamo Island and a freshwater pool in St. Petersburg (North-Western Russia) were studied with light...
Article
Full-text available
The cell body of centrohelid heliozoans is covered with a layer of scales. These scales have species-specific morphology and, since they present in the trophic stage of the cell cycle can be termed "trophic" scales. Several species are known to form cysts; during this process they can produce specific "cyst" scales, different from trophic scales. T...
Article
Centrohelid heliozoans form a monophyletic group with uncertain affinities to other groups of protists. Except for a number of naked species, they have either siliceous scales or organic spicules covering the cell. According to the phylogenetic analysis of the 18S rDNA gene, it is hypothesized that scales are the ancestral form of cell coverings, w...
Article
Three new species of centrohelid heliozoans Acanthocystis crescenta, A. kirilli, and Choanocystis minima from two freshwater lakes of Valamo Island (North-Western Russia) were studied using light- and scanning electron microscopy. The main apomorphy of A. kirilli are slightly branched radial scales. Acanthocystis crescenta has characteristic radial...

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Projects (2)
Project
Here a collection of the video records of various amoeboid protists is presented. The main URL of this collection is https://www.youtube.com/channel/UCRK3mXipI41tytxPpIC1qXQ/videos where it is regularly updated. "Amoeba" in general is a type of cell which is able to move and feed using conformations of the whole cell body and with the help of pseudopodia - temporary projections of the cytoplasm of various shapes driven by different types of the cytoskeleton. Amoeboid types of cells have evolved independently in many different branches of the eukaryotic tree of life. However, some of the branches are predominantly amoeboid. Here we collect reference light microscopic videos of different amoeboid protozoa that we find in different natural habitats during our studies of the taxonomy, phylogeny and biogeography of these groups. Videos are uploaded constantly, so check back regularly for updates. Looking forward to your feedback to: Alexander Kudryavtsev (alexander.kudryavtsev@spbu.ru). This collection is created as part of the project 15-29-02749 supported by the Russian Foundation for Basic Research.
Project
Our goal is to assemble a curated reference database spanning the eukaryotic tree of life. This will be a community resource consisting of curated sequences, flexible taxonomy, phylogenetic trees and their underlying sequence alignments. This database will increase the power of HTS-based studies to uncover fundamental patterns in microbial ecology and diversity. Along the way, individual curators are quite likely to identify novel eukaryotic clades and gain new insight into the environmental distribution patterns of eukaryotic microbes.