Vasco D.B. Bonifácio

Vasco D.B. Bonifácio
Instituto Superior Técnico · Department of Bioengineering

BEng PhD * Sustainable Nanochemistry * @sustainablenanochem
Prof. Biomaterials at Bioengineering Department, IST-ULisboa. Nanotechnology, Biomaterials and Regenerative Medicine.

About

119
Publications
14,167
Reads
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1,977
Citations
Additional affiliations
June 2021 - present
Instituto Superior Técnico
Position
  • Professor (Assistant)
January 2020 - present
Instituto Superior Técnico
Position
  • Researcher
May 2015 - present
Technical University of Lisbon
Position
  • Luminescent probes
Description
  • Oxygen and temperature sensors.

Publications

Publications (119)
Article
Blue: Biocompatible and biodegradable water-soluble dendrimers comprising ureas within the interior and amino groups on the periphery were synthesized in supercritical carbon dioxide (dendrimer of generation 1 shown in picture). This novel class of dendrimers shows a pH-dependent intrinsic blue fluorescence at very low concentrations, which makes t...
Article
Full-text available
Catalyst-free reactions developed during the last decade and the latest developments in this emerging field are summarized with a focus on catalyst-free reactions in-water and on-water. Various named reactions, multi-component reactions and the synthesis of heterocyclic compounds are discussed including the use of various energy input systems such...
Article
In the past decade, alternative benign organic methodologies have become an imperative part of organic syntheses and chemical reactions. The various new and innovative sustainable organic reactions and methodologies using no solvents or catalysts and employing alternative energy inputs such as microwaves, sonication, conventional and room temperatu...
Article
Polyurea oxide (PURO) biodendrimers are a class of dendrimers that can trigger osteogenic differentiation of human mesenchymal stem cells (hMSCs). PURO biodendrimers are prepared by simple, solventless oxidation of polyurea dendrimers using hydrogen peroxide as the oxidant in quantitative yield, retaining both biocompatibility (up to 10 mg/mL for h...
Article
The presence of genotoxic impurities in active pharmaceutical ingredients (APIs) is a major concern for the pharmaceutical industry. Acetamide is a common genotoxic byproduct found in synthetic routes of many APIs, mainly due to acetonitrile hydrolysis, and selective scavenging is a still a challenging task. Herein, as a proof-of-concept, we evalua...
Article
Full-text available
The production of medical devices follows strict guidelines where bio- and hemocompatibility, mechanical strength, and tear resistance are important features. Segmented polyurethanes (PUs) are an important class of polymers that fulfill many of these requirements, thus justifying the investigation of novel derivatives with enhanced properties, such...
Article
The efficacy of conventional antimicrobials is falling to critical levels and raising alarming concerns around the globe. In this scenery, engineered nanoparticles emerged as a solid strategy to fight growing deadly infections. Here, we show the in vitro and in vivo performance of pharmadendrimers, a novel class of engineered polyurea dendrimers th...
Article
Blocking the catalytic activity of urease has been shown to have a key role in different diseases as well as in different agricultural applications. A vast array of molecules have been tested against ureases of different species, but the clinical translation of these compounds has been limited due to challenges of potency, chemical and metabolic st...
Article
Full-text available
Cancer is the second most common cause of death worldwide, having its origin in the abnormal growth of cells. Available chemotherapeutics still present major drawbacks, usually associated with high toxicity and poor distribution, with only a small fraction of drugs reaching the tumour sites. Thus, it is urgent to develop novel therapeutic strategie...
Article
Full-text available
The activation of endothelial cells (ECs) is a crucial step on the road map of tumor angiogenesis and expanding evidence indicates that a pro-oxidant tumor microenvironment, conditioned by cancer metabolic rewiring, is a relevant controller of this process. Herein, we investigated the contribution of oxidative stress-induced ferroptosis to ECs acti...
Article
Full-text available
Direct O-alkylation of p-tert-butyldihomooxacalix[4]arene (1) with N-(bromopropyl)- or N-(bromoethyl)phthalimides and K2CO3 in acetonitrile was conducted under conventional heating (reflux) and using microwave irradiation and ball milling methodologies. The reactions afforded mono- and mainly distal di-substituted derivatives in the cone conformati...
Article
Chitosan, a natural and abundant biopolymer has been long explored as a biocompatible material for the preparation of drug delivery devices. This strategy has been mostly accomplished using chemically crosslinked chitosan leading to more stable scaffolds. However, crosslinking has been shown to reduce both biocompatibility and swelling. In this wor...
Article
Full-text available
Klebsiella pneumoniae, one of the most common pathogens found in hospital-acquired infections, is often resistant to multiple antibiotics. In fact, multidrug-resistant (MDR) K. pneumoniae producing KPC or OXA-48-like carbapenemases are recognized as a serious global health threat. In this sense, we evaluated the virulence of K. pneumoniae KPC(+) or...
Article
Full-text available
All-green matters. Synthesis of the ligand and metal–ligand complexes and a model catalytic oxidation reaction can be sequentially processed under solventless conditions using a planetary ball mill, via fast, high yielding and selective processes.
Conference Paper
Full-text available
The awareness of sustainability led to increasing global demand for processes that use lower energy, produce reduced waste, use fewer organic solvents, and offer improved selectivity. In this context, mechanochemistry re-emerged as a powerful green methodology. The conventional synthesis and functionalization of calixarenes, both on the upper and l...
Article
Full-text available
To enable survival in adverse conditions, cancer cells undergo global metabolic adaptations. The amino acid cysteine actively contributes to cancer metabolic remodelling on three different levels: first, in its free form, in redox control, as a component of the antioxidant glutathione or its involvement in protein s-cysteinylation, a reversible pos...
Article
POXylated polyurea dendrimer nanoparticles (PUREG4OOx48) are loaded with sildenafil (SDF) by a supercritical carbon dioxide–assisted (scCO2) impregnation. Further supercritical CO2‐assisted spray drying (SASD) leads to hybrid nano‐in‐micro dry powder formulations that are investigated aiming at efficient pulmonary delivery of SDF in pulmonary arter...
Chapter
Ovarian cancer is a silent cancer which rate survival mainly relays in early stage detection. The discovery of reliable ovarian cancer biomarkers plays a crucial role in the disease management and strongly impact in patient’s prognosis and survival. Although having many limitations CA125 is a classical ovarian cancer biomarker, but current research...
Article
Full-text available
: Ovarian cancer is a highly lethal disease, mainly due to chemoresistance. Our previous studies on metabolic remodeling in ovarian cancer have supported that the reliance on glutathione (GSH) bioavailability is a main adaptive metabolic mechanism, also accounting for chemoresistance to conventional therapy based on platinum salts. In this study, w...
Article
Hybrid photodiodes (HPD) systems are on the rise. The growing interest on these devices is mainly due to their power in image sensing and bioengineering solutions. In this work, a new class of donor‐acceptor dendrimers was synthesized and incorporated, along with a ruthenium sensitizer dye, into low cost devices comprised of one single layer of a b...
Article
Full-text available
Ovarian cancer is the main cause of death from gynecological cancer, with its poor prognosis mainly related to late diagnosis and chemoresistance (acquired or intrinsic) to conventional alkylating and reactive oxygen species (ROS)-generating drugs. We and others reported that the availability of cysteine and glutathione (GSH) impacts the mechanisms...
Article
Full-text available
l-Buthionine sulfoximine (l-BSO) is an adjuvant drug that is reported to increase the sensitivity of cancer cells to neoplastic agents. Dendrimers are exceptional drug delivery systems and l-BSO nanoformulations are envisaged as potential chemotherapeutics. The absorption of l-BSO at a low wavelength limits its detection by conventional analytical...
Article
A simple, clean and sustainable technology has been applied to the development of amide-based artificial receptors for a pharmaceutical impurity - acetamide. Artificial receptors in the form of Analogue Template Molecularly Imprinted Polymers (AT-MIPs) were produced in supercritical carbon dioxide (scCO2) using methacrylamide as functional monomer...
Article
The Cover Feature shows engineered nano‐in‐microparticles specially designed for inhalation chemotherapy. The dry powder formulations, produced by supercritical CO2‐assisted co‐atomization of chitosan and polyurea dendrimers, deliver anticancer drugs to the deep lung. More information can be found in the Full Paper by R. B. Restani et al.
Article
Full-text available
POxylated polyurea dendrimer (PUREG4OOx48)‐based nanoparticles were loaded with paclitaxel (PTX) and doxorubicin (DOX) and micronized with chitosan (CHT) by using supercritical CO2‐assisted spray drying (SASD). Respirable, biocompatible, and biodegradable dry powder formulations (DPFs) were produced to effectively transport and deliver the chemothe...
Conference Paper
Full-text available
In order to contribute to the access of blind and visually impaired (BVI) people to the study of chemistry, we are developing Nav-mol, an application that helps BVI chemistry students to interpret molecular structures. This application uses sound to transmit the information about the structure of the molecules. Navmol uses voice synthesis and descr...
Chapter
Polyurea and its copolymers are a versatile class of materials with many industrial applications. Over the years, the synthesis of linear, hyperbranched, and dendritic polyureas has been improved through the introduction of safe and green polymerization strategies, thus avoiding the use of shock sensitive and highly toxic intermediates. Polyureas a...
Article
A novel methodology for the determination of the numberaverage molecular weight (M-w) of linear, branched and crosslinked polymers is disclosed. The method relies on chemoenzymatic- triggered fluorescence of dormant CdTe polymercaged quantum dots synthesised in supercritical carbon dioxide. Polyurea (PURE) dendrimers (G1, G4, G5 and G6) are present...
Article
Pulmonary administration offers excellent advantages over conventional drug delivery routes, including increasing therapeutics bioavailability, and avoiding long-term safety issues. Formulations of nano-in-micro dry powders for lung delivery are engineered using (S)-ibuprofen as a model drug. These biodegradable formulations comprise nanoparticles...
Article
Gold nanoparticles (GNPs) are one of most investigated nanomaterials for lung cancer diagnosis and therapy (theragnosis). For imaging purposes, GNPs are often tagged with fluorescent probes, but unfortunately the associated plasmon resonance effect leads to fluorescence self-quenching, thus precluding accurate localization. In this study, biocompat...
Article
Full-text available
The integrated use of supercritical carbon dioxide (scCO2) and micro- and nanotechnologies has enabled new sustainable strategies for the manufacturing of new medications. 'Green' scCO2-based methodologies are well suited to improve either the synthesis or materials processing leading to the assembly of three-dimensional multifunctional constructs....
Poster
Innovative development of nano-in-micro dry powder formulations for lung cancer treatment
Article
The design and preparation of highly efficient drug delivery platforms using green methodologies is at the forefront of nanotherapeutics research. POxylated polyurea dendrimers are efficiently synthesized using a supercritical-assisted polymerization in carbon dioxide. These fluorescent, pH-responsive and water-soluble core-shell smart nanocarriers...
Article
Full-text available
Gold nanoparticles (AuNPs) are the most investigated nanomaterials for theragnosis applications. In a research field where live cell assays, as well as the tracking of nanomaterials into cell’s environment, are of extremely importance, water-soluble AuNPs have been intensively studied to overcome the toxic effects exerted by coatings. Unfortunately...
Article
Full-text available
Molecularly imprinted polymers are simple and robust materials for the selective binding of analytes with affinities and selectivities similar to biological probes. A green supercritical CO2-assisted molecular imprinting protocol enabled the production of smart sensory particles, incorporating quantum dots, with molecular recognition to bisphenol A...
Article
The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers, are ‘smart’ biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines...
Article
Polyurea dendrimer-perylene nanoshells are found to accurately discriminate structurally closely related nitroaromatics. Both studied systems, PUREG1-PER6 and PUREG4-PER48, present high sensitivity (detection limits between 5 × 10−6 and 20 × 10−6m) and selectivity for 2,4,6-trinitrotoluene (TNT) collected from a powdered military grenade and picric...
Article
This paper presents a sustainable strategy for improving the capture of antibodies by affinity chromatography. A novel biomimetic ligand (4-((4-chloro-6-(3-hydroxyphenoxy)-1,3,5-triazin-2-yl)oxy)naphthalen-1-ol) (TPN-BM) was synthesized using a greener and simple protocol to overcome solubility limitations associated with ligand 22/8, known as arti...
Data
SIRS and SSIRS files in MIDI format; chemical structures in MDL MOLfile.
Article
The Nobel Prize is the most prestigious prize in the world. With the use of online free available tools, a poster was constructed with QR-coded information about of the Nobel Prizes in chemistry from 1901 to 2011. The QR-NPchem poster can be explored as a mobile-learning tool, using a smart phone, to introduce the Nobel Prizes in Chemistry in the c...
Poster
Multifunctional PURE-type dendrimers: Sustainable Strategies to Improve Their Pharmacokinetic Performance
Article
To make the analysis of infrared (IR) spectra accessible to students who are blind and visually impaired (BVI), the visual information of the spectra was converted into nonspeech sounds using the open-source programs JDXview v0.2 and CSV to MIDI converter. In the sonified infrared spectra (SIRS), time is used as a spectral frequency indicator, and...
Poster
Pharmacokinetics study and Evaluation of nucleous internalization of IBUPROFEN-Loaded dendrimers