Vasanthraju S.G.

Manipal Academy of Higher Education | MAHE · Department of Advanced Pharmaceutical Sciences

Inulin is a high molecular weight carbohydrate made up of fructose molecules attached with glucosyl moieties in the end. Due to its high molecular weight direct analysis is tedious hence we opted for derivatization. Inulin was derivatized using Seliwanoff’s reagent and was analyzed using UV-spectrophotometry. This method was validated for specifici...

Dental diseases are one of the significant public health problems globally. Herbal medicines for managing oral diseases are considered an effective alternative to synthetic compounds due to their lower side effect. Acacia catechu, Piper nigrum, Piper longum, and Piper cubeba are vital components of Dasanakanthi Churnam used to control and prevent o...

In this present study, a new co-crystal of azithromycin with nicotinamide and naringenin has been developed with improved solubility. Azithromycin is a class II drug with poor aqueous solubility; hence an attempt has been made to improve its solubility through co-crystallization technology. In this study, the coformers selected were nicotinamide an...

Ziprasidone hydrochloride is a second-generation antipsychotic drug employed for the treatment of schizophrenia and acute mania or mixed episodes associated with bipolar disorder. During the scale-up of ziprasidone hydrochloride, an unknown impurity was observed in the batches ranging from 0.10 to 0.15% by HPLC-UV analysis. The structure of an unkn...

A simple, accurate, specific and stability-indicating RP-HPLC method was developed for simultaneous determination of chlorzoxazone, diclofenac sodium and paracetamol, using C18 Vydac Monomeric 120A (250 × 4.6mm, 5μ) at 40ºC. The mobile phase contains a mixture of 20mM potassium dihydrogen phosphate buffer (pH 6.2 adjusted with potassium hydroxide)...

Water samples were collected from 35 locations in the Arkavathi river, its tributaries and lakes spread across the Bengaluru metropolitan area in Southern India during monsoon (October 2018) and post-monsoon (February 2019) seasons. Eleven pharmaceutical and personal care products (PPCPs) (sulfamethoxazole, chloramphenicol, ethyl paraben, diclofena...

In the present analytical approach, beta-alanine (ALA) and tris-(hydroxyl methyl) aminomethane (TRIS) were investigated as peak modifiers due to their water solubility and their possible peak modifying a property. These reagents were tested for their efficacy on the elution of aceclofenac (ACF) and haloperidol hydrochloride (HLC) from C18 column (2...

In recent years for the development of novel anticancer hybrids with two or more pharmacophores of bioactive scaffolds to produce a single molecule by blending or linking has emerged as a simple strategy by providing an effective control on malignant process with improved biological potential. In this view, Sesamol being an active scaffold with imp...

The IoT in health care is currently booming in the world of health care in particular. Industry has risen from generation 1.0 to 4.0 during the Internet of things period. As we remember, we came across the exact submission of the traditional health care system. Each time the patient has needed to visit the clinic/hospitals, even for small complicat...

In this present study a new co-crystals of zoledronic acid with DL-tartaric acid and nicotinamide has been developed with improved solubility. Zoledronic acid is a class III drug with poor oral bioavailability due to its poor permeability and low aqueous solubility; hence an attempt has been made to improve its solubility by co-crystallization tech...

Purpose The goal of this study was to investigate the ability of saquinavir to generate the in vivo supersaturation and the impact of the presence of another solute, i.e., ritonavir, on the phase behavior of the former. Method The phase behavior of saquinavir alone and in the presence of ritonavir was studied by pH shift supersaturation assay. The...

A stability indicating RP-HPLC method was developed for the simultaneous estimation of propranolol hydrochloride and flunarizine dihydrochloride in bulk and various fixed-dose combinations in the presence of its degradation products. The validation of the method was performed as per ICH Q2(R1) and USFDA guidelines. The separation was achieved with...

Continuous improvement and advancement in Pharmaceutical manufacturing industry are a paramount part of FDA’s mission to safeguard and encourage public wellness. Product recalls are increasing despite elaborate QA/QC monitoring by most companies. Quality metric is the quantification of systems’ effectiveness connected through the manufacturing of d...

A stability indicating RP-HPLC method was developed for the simultaneous estimation of propranolol hydrochloride and flunarizine dihydrochloride in bulk and various fixed-dose combinations in the presence of its degradation products. The validation of the method was performed as per ICH Q2(R1) and USFDA guidelines. The separation was achieved with...

Introduction: The new ferrous bisglycinate effervescent formulation is a combination of iron and ascorbic acid where iron is in complex form with glycine. No analytical method was reported so far for the analysis of iron and ascorbic acid in effervescent tablets. Hence, an attempt was made to develop and validate the colorimetric and RP-HPLC method...

The promising role of thiazolidin-4-ones (TZOs) against inflammatory conditions has been reported. From our lab, one of the TZO derivatives, compound 4C, exerted anti-inflammatory potential via inhibition of locally released cytokines and prostaglandin. In continuance, a detailed study was undertaken for the preclinical profiling of this promising...

Clinical Trials exhibit the nature of drug which ensures the safety and efficiency of Pharmaceutical products in the various segments of its use. Monitoring adverse reactions are important mechanism ensuring the safety. Clinical researches effectively help the reliability of research and development design ensuring the safety of the subject exposed...

We synthesized twenty thiazolidin-4-one derivatives, which were then characterized by standard chromatographic and spectroscopic methods. From the in vitro glucose uptake assay, two compounds behaved as insulin sensitizers, where they enhanced glucose uptake in isolated rat diaphragm. In high-carbohydrate diet-induced insulin resistant mice, these...

Three thiazolidin-4-one derivatives were synthesized, purified and characterized by chromatographic and spectroscopic methods. In the in vitro assays, these compounds inhibited reactive oxygen species (ROS), nitrite and cytokine generation in RAW 264.7 murine macrophages and whole blood. These derivatives attenuated carrageenan-induced acute inflam...

sensitive, selective, fast and validated isocratic reversed-phase HPLC method for simultaneous determination of the new generation antiepileptic drugs, lamotrigine (CAS 84057-84-1) and felbamate (CAS 25451-15-4) in human plasma using spectrophotometric detection is presented in this paper. Lamotrigine, felbamate and the internal standard nevirapine...

A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for quantification of lacosamide in rat plasma. A liquid-liquid extraction procedure was optimized to extract lacosamide from rat plasma. Chromatographic separation was accomplished using a reversed phase C18 Hichrom (250×4.6 mm, 5 µm) column...

A simple, sensitive, and accurate high performance liquid chromatographic method developed and validated for the simultaneous estimation of domperidone, paracetamol, esomeprazole, and lansoprazole in pharmaceutical dosage forms.The assay involved the use of photo diode array detection at 285 nm for domperidone, 246 nm for paracetamol, 300 nm for es...

A simple, highly sensitive, precise and accurate high-performance liquid chromatographic (LCMSMS) method with mass detection was developed and validated for the rapid quantification of flupirtine (CAS 75507-68-5) in rat plasma samples. The chromatographic separation was achieved with a reverse phase column (4.6 x 50 mm, 5 microm) and the mobile pha...

A simple, selective, precise, stability-indicating HPTLC method has been established for analysis of tolterodine in the bulk drug. Chromatography on aluminum foil-backed silica gel 60GF-254 plates with toluene-methanol-aqueous ammonia 5:5:0.02 (v/v) as mobile phase gave a compact spot at R(F) 0.40 +/- 0.02. Densitometric analysis of tolterodine was...

Bioequivalence of drug formulation is a vital concern in drug development and even more significant in the case of Narrow Therapeutic Index (NTI) drugs. In clinical development of New Chemical Entities (NCE), bioequivalence studies necessitate to be performed when the formulation of the pharmaceutical dosage form has been changed. In vivo pharmacok...

A stability indicating, isocratic, rapid, specific, and sensitive high performance liquid chromatographic (HPLC) method was developed and validated for determination of Solifenacin in bulk drug. The quantitative determination of SOLIFENACIN was performed on a Hypersil C 8 (250mm × 4.6 mm i.d., 5 μm) column with mobile phase consist of 10 mM potassi...

Cosmetics market has been dramatically improving around the world. Factors for successful marketing should take into consideration the current market trends & demands, regulatory framework & compliance requirements, efficacy, safety and scientific learning on performance. The current regulations of cosmetics are stringent. There are different regul...

A series of 19 heterocyclic homoprostanoids were synthesized from easily available oleic and ricinoleic acids and evaluated for their possible antioxidant, anti-inflammatory and anti-hyperlipidaemic activities. Compounds with thioxo- and oxoimidazole ring (1) and (2) have shown potent antioxidant activity with IC(50) values 0.23±0.09 and 0.41±0.01m...

A regulation with respect to combination products and FDA over view. Advances in drug, biologic and medical device development relate to both single entities and combination of each type of medicinal product. Regulatory overview of combination products is complicated by the fact that although drugs are regulated primarily under section 505 of Food,...

Regulations for pharmaceutical bulk excipients are stringent as same as pharmaceutical medicines. In case of new excipient, applicant has to submit safety & quality data. In case of approved or existed excipient applicant has to submit literature reference data i. e. FDA inactive ingredients guide, the national formulary, U.K. ABPI data sheets as p...

A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C 18 (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile...

A simple, precise and rapid RP-HPLC method was developed for the determination of Granisetron hydrochloride in pharmaceutical formulation. The method was carried out on a phenomenex RP-C18 column using a mixture of methanol and water (75:25). Detection was done at 301nm. The linearity range was 5 - 100.0mcg/mL. The proposed method was statistically...

A simple, rapid, and accurate RP-HPLC method was developed for the determination of tolterodine in solid pharmaceutical dosage form. The method showed a linear response for concentration in the range of 1-15μg/ml using acetonitrile:potassium dihydrogen phosphate (50:50,v/v) as the mobile phase with detection at 284 nm and a flow rate of 1ml/min. Th...

A simple, rapid and accurate RP-HPLC method was developed for the determination of racecadotril in solid pharmaceutical dosage form. The method showed a linear response for concentrations in the range 10-200 μg/ml using methanol: water (80:20 v/v) as the mobile phase with detection at 231 nm and a flow rate of 1.2 ml/min. Solution concentrations we...

A simple, precise and rapid fluorimetric method was developed for the determination of trifluoperazine hydrochloride in pharmaceutical dosage forms. Spectrofluorimetric method is based on the measurement of the native fluorescence and has always proved selective with relatively low detection limits. Trifluoperazine hydrochloride showed strong nativ...

A simple accurate, sensitive reproducible spectrofluorimetric method was developed for the analysis of citalopram in pure and pharmaceutical dosage form. Citalopram showed strong native fluorescence in 0.05 M sulphuric acid having excitation at 239 nm and emission at 300 nm. All parameters like the effect of different solvents, pH, dilutions, react...

A sensitive and selective HPLC method was developed for quantification of duloxetine, in rat plasma. Trifluoperazine was used as an internal standard (IS). The present method used protein precipitation for extraction of the drug from rat plasma. Separation and quantification was carried using in isocratic mode using 25 mM phosphate buffer (pH 3.0)/...