Varvara Gribova

University of Strasbourg | UNISTRA · Faculty of Dental surgery

Implantation of biomedical devices is often followed by bacterial infections that may seriously affect implant functionalities and lead to their failure. In the context of bacterial resistance to antibiotics, which is a growing problem worldwide, new strategies that are able to overcome these problems are needed. In this work, we introduce a new fo...

Layer-by-layer (LbL) deposition method of polyelectrolytes is a versatile way of developing functional nanoscale coatings. Even though the mechanisms of LbL film development are well-established, currently there are no predictive models that can link film components with their final properties. The current health crisis has shown the importance of...

Implants and prostheses are widely used to either repair damaged tissues or treat different diseases. Before an implant reaches the market, multiple preclinical and clinical tests must be performed. Along with cytotoxicity or hemocompatibility preclinical tests, genotoxicity is an essential feature to investigate. Indeed, the materials used for imp...

Tissue damage due to cancer, congenital anomalies, and injuries need new efficient treatments that allow tissue regeneration. In this context, tissue engineering shows a great potential to restore the native architecture and function of damaged tissues, by combining cells with specific scaffolds. Scaffolds made of natural and/or synthetic polymers...

Layer-by-layer film (LbL) coatings made of polyelectrolytes are a powerful tool for surface modification, including the applications in the biomedical field, for food packaging, and in many electrochemical systems. However, despite the number of publications related to LbL assembly, predicting LbL coating properties represents quite a challenge, ca...

Ovarian cancer remains a major public health issue due to its poor prognosis. To develop more effective therapies, it is crucial to set-up reliable models that closely mimic the complexity of the ovarian tumor's microenvironment. 3D bioprinting is currently a promising approach to build heterogenous and reproducible cancer models with controlled sh...

The surface stiffness of the microenvironment is a mechanical signal regulating biofilm growth without the risks associated with the use of bioactive agents. However, the mechanisms determining the expansion or prevention of biofilm growth on soft and stiff substrates are largely unknown. To answer this question, we used PDMS (polydimethylsiloxane,...

The engineering of skeletal muscle tissue, a highly organized structure of myotubes, is promising for the treatment of muscle injuries and muscle diseases, for replacement, or for pharmacology research. Muscle tissue development involves differentiation of myoblasts into myotubes with parallel orientation, to ultimately form aligned myofibers, whic...

The engineering of skeletal muscle tissue, a highly organized structure of myotubes, is promising for the treatment of muscle injuries and muscle diseases, for replacement, or for pharmacology research. Muscle tissue development involves differentiation of myoblasts into myotubes with parallel orientation, to ultimately form aligned myofibers, whic...

A versatile 3D bioprinted pancreas tumor model for new anticancer drug development C. Godier, Z. Baka, A. Mallick, V. Gribova, É. Gaffet, H. Alem ESB 2022 - 32nd Annual Conference of the European Society of Biomaterials Session B-03 Bioprinting and bioinks – Poster P19 4 – 8 Septembre 2022, Bordeaux (France) http://www. https://www.esbbordeaux2022....

3D bioprinting to build heterotypic tumor models recaputilating the tumor microenvironment C. Godier, Z. Baka, V. Gribova, L. Lamy, L. Bolotine, E. Gaffet, H. Alem EuroOcs Conference 2022, Annual Meeting 4 au 5 juillet 2022, Grenoble (France) https://euroocs.eu/annual-meeting/

"Construction de modèle de tumeur ovarienne par bio-impression 3D pour le développement de nouveaux médicaments anticancéreux" C. Godier, Z. Baka, A. Mallick, V. Gribova, L. Lamy, L. Bolotine, É. Gaffet, Halima Alem Séminaire Ecole Doctorale C2MP- Université de Lorraine 16 Juin 2022, Nancy, France

3D bioprinting is considered as a promising technology to build living-like models. 3D constructs containing different cell types can be generated which is crucial to simulate the heterogeneity and complexity of the human cells microenvironment. As, 3D bioprinting is a computer assisted process that generates 3D structures with a controlled archite...

Implantation of biomedical devices is followed by immune response to the implant, as well as occasionally bacterial, yeast and/or fungal infections. In this context, new implant materials and coatings that deal with medical device‐associated complications are required. Antibacterial and anti‐inflammatory materials are also required for wound healin...

Implants and prostheses are widely used to replace damaged tissues or to treat various diseases. However, besides the risk of bacterial or fungal infection, an inflammatory response usually occurs. Here, recent progress in the field of anti-inflammatory biomaterials is described. Different materials and approaches are used to decrease the inflammat...

To fight against antibiotic‐resistant bacteria adhering and developing on medical devices, which is a growing problem worldwide, researchers are currently developing new “smart” materials and coatings. They consist in delivery of antimicrobial agents in an intelligent way, i.e., only when bacteria are present. This requires the use of new and sophi...

In this study, we used a recently developed approach of coating the cells with fibronectin-gelatin nanofilms to build 3D skeletal muscle tissue models. We constructed the microtissues from C2C12 myoblasts and subsequently differentiated them to form muscle-like tissue. The thickness of the constructs could be successfully controlled by altering the...

Controlling the different steps of cell differentiation in vitro using bioactive surfaces may be useful in view of future cell therapies. Substrates presenting peptides, which are minimal fragments of extracellular matrix (ECM) proteins may be used for this purpose. In this work, we used polyelectrolyte multilayer films presenting two peptides deri...

Mesenchymal Stem Cells (MSCs) with osteoblastic/chondrocytic potential have been detected in different anatomical sites including the Fetal Liver (FL). The puzzling presence of such cells in FL led us to investigate whether they displayed additional differentiation competence compatible with their location at this developmental stage. This study ha...

Skeletal muscle tissue engineering holds promise for the replacement of muscle due to an injury and for the treatment of muscle diseases. Although RGD substrates have been widely explored in tissue engineering, there is no study aimed at investigating the combined effects of RGD nanoscale presentation and matrix stiffness on myogenesis. In the pres...

Controlling the bulk and surface properties of materials is a real challenge for bioengineers working in the fields of biomaterials, tissue engineering and biophysics. The layer-by-layer (LbL) deposition method, introduced 20 years ago, consists in the alternate adsorption of polyelectrolytes that self-organize on the material’s surface, leading to...

Cells respond to a variety of stimuli, including biochemical, topographical and mechanical signals originating from their micro-environment. Cell responses to the mechanical properties of their substrates have been increasingly studied for about 14 years. To this end, several types of materials based on synthetic and natural polymers have been deve...