Vanessa Fernandes Cardoso

Vanessa Fernandes Cardoso
University of Minho · CMEMS and CFUM, University of Minho (Portugal)

PhD in Biomedical Engineering

About

72
Publications
11,105
Reads
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1,827
Citations
Additional affiliations
March 2014 - February 2020
University of Minho
Position
  • PostDoc Position
March 2013 - present
University of Minho
Position
  • PostDoc Position
Description
  • Lab-on-a-chip for the quantification of iodine in human urine
October 2010 - February 2013
Cooperative Higher Educational, Polytechnic and University
Position
  • Professor (Associate)
Description
  • Teacher and supervisor of internships in various healthcare provider services of the biomedical engineering undergraduate course
Education
December 2010 - December 2010
Fraunhofer Institute for Biomedical Engineering
Field of study
  • Microfluidics
October 2008 - May 2009
University of Minho
Field of study
  • Micro and Nano Technology Master
October 2008 - December 2012
University of Minho
Field of study
  • Biomedical Engineering

Publications

Publications (72)
Article
Portable analytical systems are versatile tools for application in areas including biomedicine, biosecurity, food safety and environmental monitoring. This work contributes to the increasing demand for low-cost, environmentally friendly substrates for portable analytical systems by using natural Bombyx mori cocoons. Further, silk fibroin is also ex...
Article
Microfluidic paper-based analytical devices (µPADs) have gained substantial attention as portable analytical devices in various (bio)technological fields, for being affordable, user-friendly, portable, energy efficient and for allowing multiplexed analysis. Although significant progress has been achieved, there is still need for improvement in term...
Article
Full-text available
Stimuli-responsive liposomes are a class of nanocarriers whose drug release occurs, preferentially, when exposed to a specific biological environment, to an external stimulus, or both. This work is focused on the design of solid magnetoliposomes (SMLs) as lipid-based nanosystems aiming to obtain multi-stimuli-responsive vesicles for doxorubicin (DO...
Article
Full-text available
Biomimicking biological niches of healthy tissues or tumors can be achieved by means of artificial microenvironments, where structural and mechanical properties are crucial parameters to promote tissue formation and recreate natural conditions. In this work, three-dimensional (3D) scaffolds based on woodpile structures were fabricated by two-photon...
Chapter
Organic piezoelectric materials stand out compared to ceramic materials due to their lightweight, flexibility, and chemical and mechanical resistance and are applied in an increasing number of applications, from sensors and actuators, energy harvesting and storage, biomedical to environmental areas. The present chapter focuses on organic piezoelect...
Article
Full-text available
This work reports on the production of poly(vinylidene fluoride) (PVDF) membranes by non-solvent induced phase separation (NIPS) using N,N-dimethylformamide (DMF) as solvent and water as non-solvent. The influence of the processing conditions in the morphology, surface characteristics, structure, thermal and mechanical properties were evaluated for...
Article
Portable analytical systems are increasingly required for clinical analysis or environmental monitoring, among others, being materials with tailored physicochemical properties among the main needs for successful functional implementation. This article describes the processing of fluorinated poly(vinylidene-co-trifluorethylene), P(VDF-TrFE), membran...
Article
In order to improve battery performance by tuning battery separator membranes, this work reports on porous poly(vinylidene fluoride-co-trifluoroethylene) - P(VDF-TrFE)- membranes with surface pillar microstructures. Separators with tailored pillar diameter, height and bulk thickness were fabricated by template patterning and computer simulations, a...
Article
Full-text available
The morphological clues of scaffolds can determine cell behavior and, therefore, the patterning of electroactive polymers can be a suitable strategy for bone tissue engineering. In this way, this work reports on the influence of poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) electroactive micropatterned scaffolds on the proliferation...
Article
Tissue engineering (TE) is a strongly expanding research area. TE approaches require biocompatible scaffolds, cells, and different applied stimuli, which altogether mimic the natural tissue microenvironment. Also, the extracellular matrix serves as a structural base for cells and as a source of growth factors and biophysical cues. The 3D characteri...
Chapter
A poly(vinylidene fluoride) (PVDF) and its copolymers are polymers that in specific crystalline phases, show high dielectric and piezoelectric values, excellent mechanical behavior, and good thermal and chemical stability, suitable for many applications from the biomedical area to energy devices. This chapter introduces the main properties, process...
Article
Novel microfluidic substrates based on electrospun poly(l-lactic acid) (PLLA) membranes were developed to increase the limited range of commercially available paper substrates, commonly used for the fabrication of microfluidic paper-based analytical devices (µPADs). PLLA advantageous properties include being biodegradable, biocompatible, easily pro...
Article
Poly(vinylidene fluoride‐co-trifluoroethylene) (P(VDF-TrFE)) microspheres were obtained by a nanoprecipitation method based on the deposition of a polymeric solution over a non-solvent (NS) solution. The effect of several parameters on sphere formation was evaluated, including copolymer concentration, NS content (methanol/water ratio) and surfactan...
Chapter
Polydimethylsiloxane (PDMS) is one of the most widely used polymers in microfluidics. Furthermore, magnetic nanoparticles (MNPs), due their superior thermal properties, are also gaining a great interest among the industry and microfluidic scientific community. In this work, a technique based on a flow focusing principle was used to produce magnetic...
Article
The increasing demand on electronic and portable devices requires battery system with improved energy storage capacity. Thus, studies in all battery components are being carried out to increase their performance. One of those battery components in the separator membrane. The present work reports on porous poly(vinylidene fluoride-co-trifluoroethyle...
Article
Electroactive polymers are being increasingly used in tissue engineering applications. Together with the electromechanical clues, morphological ones have been demonstrated to determine cell proliferation and differentiation. This work reports on the micro patterning of poly(vinylidene fluoride-co-trifluoroethylene) - P(VDF-TrFE) scaffolds - and the...
Article
Separator membranes based on silica/poly(vinylidene fluoride) composites were prepared by a non-solvent induced phase separation (NIPS) process with different air exposure times before immersion in a water coagulation bath and for the same filler content of 20 wt%. Mesoporous silica spheres (SS) of ~ 400 nm average diameter were synthetized by sol-...
Article
The fabrication of highly transparent piezoelectric transducers is of increasing interest in the scientific and technological community. In this work, polymer based piezoelectric transducers were fabricated by the direct deposition of each layer forming the transducer on the desired substrate, avoiding the use of coupling layers. Transparent conduc...
Article
Poly(vinylidene fluoride-trifluoroethylene), P(VDF-TrFE), microstructures have been produced using different solvents, including green ones, by different techniques, such as solvent casting, screen-printing, replica molding, electropray and electrospinning. The obtained microstructures span from simple porous and dense films, to spheres, fibers and...
Article
Poly(vinylidene fluoride) (PVDF) and its copolymers are the polymers with the highest dielectric constants and electroactive responses, including piezoelectric, pyroelectric and ferroelectric effects. This semicrystalline polymer can crystallize in five different forms, each related to a different chain conformation. Of these different phases, the...
Article
Full-text available
Fluorinated polymers constitute a unique class of materials that exhibit a combination of suitable properties for a wide range of applications, which mainly arise from their outstanding chemical resistance, thermal stability, low friction coefficients and electrical properties. Furthermore, those presenting stimuli-responsive properties have found...
Chapter
Electroactive polymers (EAP) are actuators that most closely emulate biological muscles compared to any other actuators that are human-made; therefore they earned the moniker “artificial muscles.” The materials that were developed in the early days of this field generated limited actuation strain and therefore received relatively little attention....
Article
Magnetic nanoparticles (NPs) are emerging as an important class of biomedical functional nanomaterials in areas such as hyperthermia, drug release, tissue engineering, theranostic, and lab-on-a-chip, due to their exclusive chemical and physical properties. Although some works can be found reviewing the main application of magnetic NPs in the area o...
Article
The successful application of magnetic nanoparticles (MN) is highly dependent of their effective cleaning after synthesis and/or before their use in order to remove solvents, excess of surfactants, byproducts and undesired impurities that can be toxic and/or cause side effects, hindering their applicability. As manual cleaning is a time consuming a...
Article
Zinc-decorated magnetic silica spheres were developed, optimized and tested for the capture and separation of L‐histidine. The magnetic silica spheres were prepared using a simple sol-gel method and show excellent magnetic characteristics, adsorption capacity toward metal ions, and stability in aqueous solution in a wide pH range. The binding capac...
Chapter
The area of smart and functional materials is receiving increasing interest in terms of scientific and technological applications. In particular, those smart materials able to change form as a function of specific environments or physicochemical inputs-metamorphic materials-are relevant for medical applications including tissue engineering or drugs...
Article
Polymer composite membranes based on poly(vinylidene fluoride-hexafluoropropylene), PVDF-HFP, with different aluminum hydroxide filler content, bayerite polymorph, were prepared by solvent casting for arsenic removal from water by filtration and adsorption. Morphological, structural and mechanical characterization of the membranes were performed. T...
Article
A direct, systematic and comparative study on the production of magnetic silica spheres (MSS) with control over their most relevant parameters, including size, morphology and magnetization, is presented. MSS were prepared by a simple and efficient sol-gel method varying different synthesis parameters. Porous and non-porous MSS with sizes from ∼110...
Article
The manufacture of three-dimensional patterned electroactive poly(vinylidene fluoride-co-chlorotrifluoroethylene) microstructures with tailored architecture, morphology, and wettability is presented. The patterned microstructures are fabricated using a simple, effective, low cost, and reproducible technique based on microfluidic technology. These n...
Article
Green chemistry approaches are increasingly relevant for the development of sustainable technologies, but this approach is scarcely being used in the field of piezoelectric polymers. Poly(vinylidene fluoride) (PVDF) based materials are the family of polymers with the larger piezoelectric response and, when processed from solution, are typically pre...
Article
Poly(vinylidene fluoride-. co-chlorotrifluoroethylene) - P(VDF-CTFE) membranes are increasingly interesting for a wide range of applications, including battery separators, filtration membranes and biomedical applications. This work reports on the morphology, hydrophobicity, thermal and mechanical properties variation of P(VDF-CTFE) membranes proces...
Article
Full-text available
A systematic study for the production of porous poly(vinylidene fluoride-trifluoroethylene), P(VDF-TrFE), films using solvent evaporation and nonsolvent induced phase separation techniques is presented. Processing parameters such as copolymer volume fraction, solvent, preset exposure time to air before immersion, and nonsolvent and temperature of t...
Article
This paper presents a systematic study for the production of poly(vinylidene fluoride–hexafluoropropylene) [P(VDF–HFP)], porous films using solvent evaporation (SE) and non-solvent-induced phase separation (NIPS) techniques. Parameters such as volume fraction of the copolymer solution, film thickness, time exposure to air, non-solvent, and temperat...
Article
Full-text available
The study and optimization of epoxy-based negative photoresist (SU-8) microstructures through a low-cost process and without the need for cleanroom facility is presented in this paper. It is demonstrated that the Ultraviolet Rays (UV) exposure equipment, commonly used in the Printed Circuit Board (PCB) industry, can replace the more expensive and l...
Article
This paper reports on a polymer-based piezoelectric transducer used in microfluidic structures for generating acoustic waves, and consequently, the acoustic streaming phenomenon, that will improve the mixing of fluids. The piezoelectric transducer is based on a poly(vinylidene fluoride-trifluoroethylene), P(VDF-TrFE), copolymer with conductive tran...
Article
Poly(vinylidene fluoride), PVDF, films have been processed by spin-coating with controlled thickness by multilayer film deposition. Films with thickness ranging from 300 nm to 25 μm, β-phase content up to 80%, piezoelectric coefficient d33 up to ∼−20 pC/N and an optical transmittance in the visible range higher than 70%, were obtained with a one to...
Article
This paper reports on a mixing system for microfluidic applications based on the acoustic streaming phenomenon. The system is based on an optimized piezoelectric transducer comprising a 25 μm thick poly(vinylidene fluoridetrifluoroethylene), P(VDF-TrFE), copolymer film with 85 nm thick electrodes of aluminium-doped zinc oxide (AZO). The system was...
Article
Poly(vinylidene fluoride–trifluoroethylene), P(VDF–TrFE), thin-films have been processed by spin-coating with controlled thickness. The influence of the thermal annealing and poling conditions on the properties of the material has been investigated. It is shown that thermal annealing strongly influences the microstructure and ferroelectric phase tr...
Article
Poly(vinylidene fluoride-trifluorethylene) porous membranes were prepared by solvent evaporation at room temperature. The morphology of the membranes reveals an interconnected porous structure with porosity ranging from 72% up to 80%. The conductivity of the membranes and the LiClO4 uptake increases with increasing porosity. After uptake, the incre...
Article
Ferroelectric macroporous poly(vinylidene fluoride-trifluoroethylene) membranes have been produced by isothermal crystallization from the solution at different temperatures starting from different diluted solutions of the co-polymer in dimethylformamide. In this way pore architecture, consisting in interconnected spherical pores can be obtained. Th...
Article
Full-text available
Poly(vinylidene fluoride), PVDF, thin films have been processed by spin-coating with controlled thickness, morphology and crystalline phases. The influence of the polymer/solvent mass ratio of the solution, the rotational speed of the spin-coater and the temperature of crystallization of the films on the properties of the material has been investig...
Article
Full-text available
Electrodes are widely used to record biological signals from living tissue, namely in neuroscience where researchers need to record brain signals in vivo. This paper presents a rapid low-cost prototyping technology for protocol-specific electrode designs. The suggested fabrication process combines SU-8 photoresist patterning in multiple layers with...
Conference Paper
Microelectrodes are widely used to record biological signals from living tissue, namely in neuroscience where researches need to record brain signals in vivo. Several types of microelectrodes are available in the market. Unfortunately, most of the microelectrode developing processes are time consuming and expensive. This work presents a rapid low-c...
Conference Paper
The research team is focused on lab-on-a-chip devices for clinical applications, with on-chip integration of electronic circuits, optical filters and (bio)sensors. An example of the work is a lab-on-a-chip where acoustic streaming technique is used to promote the mixing and the pumping of the fluids inside microchannels. The transducer that generat...
Conference Paper
This article describes an acoustic microagitator for being used in biological fluids analysis. It is known that a piezoelectric transducer, with its vibration, can be used for mixing fluids. However, in this case, the piezoelectric transducer is also used to heat the samples, improving the reaction of fluids that benefit from that effect. The piezo...
Article
Full-text available
Biochemical analysis of physiological fluids using, for example, lab-on-a-chip devices requires accurate mixing of two or more fluids. This mixing can be assisted by acoustic microagitation using a piezoelectric material, such as the β-phase of poly(vinylidene fluoride) (β-PVDF). If the analysis is performed using optical absorption spectroscopy an...
Article
This paper reports a fabrication method for producing piezoelectric poly (vinylidene fluoride) films in their electroactive β-phase that features controlled thickness, smooth and flat surface, and high transparency. These piezoelectric films are suitable for being used as integrated microactuators, such as piezoelectric pumps and/or mixers, in micr...
Article
This paper reports a fully integrated disposable lab-on-a-chip with acoustic microagitation based on a piezoelectric ??-poly(vinylidene fluoride) (??-PVDF) polymer. The device can be used for the measurement, by optical absorption spectroscopy, of biochemical parameters in physiological fluids. It comprises two dies: the fluidic die that contains t...
Article
This paper describes an on-chip pumping system and the correspondent driving circuit. The system can be used in lab-on-a-chip applications for pumping the fluids in the microchannels. The device is constituted by two chips placed together: a CMOS chip with the electronics and a glass chip, with the microchannels and the pumping system. This pumping...
Article
The healthcare system requires new devices for a rapid monitoring of a patient in order to improve the diagnosis and treatment of various diseases. Accordingly, new biomedical devices are being developed. In this paper, a fully-integrated biological microdevice for uric acid analysis in human saliva is presented. It is based on optical spectrophoto...
Conference Paper
This paper describes a lab-on-a-chip device with acoustic thermoagitation based on a piezoelectric βPVDF polymer.The device is used for testing and monitoring biochemical parameters in biological fluids using optical absorption spectrophotometry.Experimental results regarding the influence of the electrical signal amplitude and frequency applied fo...
Article
This paper describes the implementation of a smart-optical detector array for detection and concentration measurement of biochemical parameters in physiological fluids. Its application is in the low-cost microchip size analytical laboratories that use colorimetric detection, by optical absorption, as the analytical technique. The microlaboratory st...
Article
Full-text available
This paper describes a fully-integrated lab-on-a-chip device for testing and monitoring biochemical parameters in biological fluids. The major innovation of this microdevice is the application of an acoustic microagitation technique with automatic electronic control based on a β-PVDF piezoelectric polymer placed underneath the microfluidic structur...
Conference Paper
The main objective of this paper is to describe a fluidic acoustic microagitation system based on a Poly(Vinylidene Fluoride) polymer. This system benefits from the high piezoelectric properties presented by the beta-phase of the polymer (beta-PVDF) converting an electrical signal into acoustics vibrations. These vibrations can be used in microflui...
Conference Paper
Full-text available
The main objective of this article is to describe the development of a fully-integrated disposable lab-on-a- chip for point of care testing and monitoring of biochemical parameters in biological fluids. The lab-on-a- chip is composed mainly by two dies: the fluid and the detection. The fluid die, fabricated in SU-8, comprises three microfluidic cuv...
Article
This paper reports on the incorporation and validation of a microagitation system based on a piezoelectric polymer, Poly(vinylidene fluoride) in its beta phase, β-PVDF, in a fully-integrated disposable lab-on-a-chip for point-of-care testing and monitoring of biochemical parameters in biological fluids. The lab-on-a-chip concept offers a novel appr...

Questions

Questions (2)
Question
Dear all,
Does anyone know a thermochromic liquid that produces a color gradient in a temperature range between 20 and 50°C?
Thank you
Question
Dear all,
I need to obtain superhydrophilic PVDF-based membranes. Anyone know a simple method to do so? I tryed by incorporating zeolites on the PVDF-based solution but it do not work since the memebrane remains hydrophobic. I do not want to change the morphology of the pores and I really need like one step method (preferably).
Thank you,
Vanessa Cardoso

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