Valery Victorovich Tuchin

Saratov State University · Department of Optics and Biophotonics

The need for searching for new methods of antitumor therapy is due to the sharp increase in morbidity and mortality from cancer, as well as because of the fact that conventional chemo- and radiotherapy are often insufficiently effective owing to the development of tumor cells resistance. Plasmonic photothermal therapy (PPT) is a method based on tum...

XXVIII Международной школы для студентов и молодых ученых по оптике, лазерной физике и биофотонике, который состоится 26 сентября в 14.00 в Большой физической аудитории III корпуса СГУ имени Н.Г. Чернышевского «БИОФОТОНИКА-НОВАЯ НАУКА НА СЛУЖБЕ ЧЕЛОВЕЧЕСТВА» Лектор: Член-корреспондент РАН, заслуженный деятель науки РФ, профессор РФ 2022 г., доктор...

The main goal of SFM'24 is to present and discuss the latest advances in the field of biophysics of optical and laser technologies in biology and medicine, fine mechanics and control of optical and physiological properties of tissues and cells, coherent optics of random and ordered media, materials and environmental sciences, nonlinear dynamics of...

Fundus photography (FP) is a crucial technique for diagnosing the progression of ocular and systemic diseases in clinical studies, with wide applications in early clinical screening and diagnosis. However, due to the nonuniform illumination and imbalanced intensity caused by various reasons, the quality of fundus images is often severely weakened,...

The study encompasses an investigation of optical, photothermal and biocompatibility properties of a composite consisting of golden cores surrounded by superparamagnetic CoFe2O4 nanoparticles. Accompanied with the experiment, the computational modeling reveals that each adjusted magnetic nanoparticle redshifts the plasmon resonance frequency in gol...

Fundus photography (FP) is a crucial technique for diagnosing the progression of ocular and systemic diseases in clinical studies, with wide applications in early clinical screening and diagnosis. However, due to the non-uniform illumination and imbalanced intensity caused by various reasons, the quality of fundus images is often severely weakened,...

Application of optical coherence tomography (OCT) in neurosurgery mostly includes the discrimination between intact and malignant tissues aimed at the detection of brain tumor margins. For particular tissue types, the existing approaches demonstrate low performance, which stimulates the further research for their improvement. The analysis of speckl...

Using a photosensitizer (PS), light, and oxygen, photodynamic therapy creates cytotoxic reactive oxygen species, such as singlet oxygen (¹O2), that kill cancer cells. Many cancer cell lines have up to 300 times more folic acid receptors than healthy cells. Therefore, folic acid is often used to improve selectivity of PSs. Photobleaching poses a dis...

Inflammatory dermatoses represent a global problem with an increasing prevalence and recurrence among the world population. Topical glucocorticoids (GCs) are the most commonly used anti-inflammatory drugs in dermatology due to...

Optical clearing agents (OCAs) are substances that temporarily modify tissue's optical properties, enabling better imaging and light penetration. This study aimed to assess the impact of OCAs on the nail bed and blood using in vivo and in vitro optical methods. In the in vivo part, OCAs were applied to the nail bed, and optical coherence tomography...

The broadband absorption coefficient spectrum of the rabbit lung presents some particular characteristics that allow the identification of the chromophores in this tissue. By performing a weighted combination of the absorption spectra of water, hemoglobin, DNA, proteins and the pigments melanin and lipofuscin, it was possible to obtain a good match...

In this paper, we study a pattern formation in the epidermal layer of skin during tumor development and appearance of a binary surface consisting of healthy and cancer cells forming Turing patterns under external osmotic pressure. The basic methodology of introducing the external influences, for example, time-targeted drug therapy or radiation expo...

With the objective of developing new methods to acquire diagnostic information , the reconstruction of the broadband absorption coefficient spectra (μ a [λ]) of healthy and chromophobe renal cell car-cinoma kidney tissues was performed. By performing a weighted sum of the absorption spectra of proteins, DNA, oxy-genated, and deoxygenated hemoglobin...

The brain diseases account for 30% of all known diseases. Pharmacological treatment is hampered by the blood–brain barrier, limiting drug delivery to the central nervous system (CNS). Transcranial photobiomodulation (tPBM) is a promising technology for treating brain diseases, due to its effectiveness, non-invasiveness, and affordability. tPBM has...

Photodynamic therapy (PDT) is a well-established cancer treatment method that employs light to generate reactive oxygen species (ROS) causing oxidative damage to cancer cells. Nevertheless, PDT encounters challenges due to its oxygen-dependent nature, which makes it less effective in hypoxic tumor environments. To address this issue, we have develo...

Correct classification of skin lesions is a key step in skin cancer screening, which requires high accuracy and interpretability. This paper proposes a multimodal method for differentiating various clinical forms of basal cell carcinoma and benign neoplasms that includes machine learning. This study was conducted on 37 neoplasms, including benign n...

We studied grafted tumors obtained by subcutaneous implantation of kidney cancer cells into male white rats. Gold nanorods with a plasmon resonance of about 800 nm were injected intratumorally for photothermal heating. Experimental irradiation of tumors was carried out percutaneously using a near‐infrared diode laser. Changes in the optical propert...

Using an optical coherence tomograph, the results of immersion optical clearing of human skin in vivo using an aqueous solution of glucosamine hydrochloride as a optical clearing agent were obtained. To assess the effectiveness of optical clearing, we determined the rate of decrease in the light scattering coefficient obtained using an averaged A-s...

Final Version of Patent

The biggest obstacle to optical imaging is light attenuation in biological tissues. Conventional clearing techniques, such as agent-based clearing, improve light penetration depth by reducing scattering, but they are hampered by drawbacks including toxicity, low efficiency, slowness, and superficial performance, which prevent them from resolving th...

The results of in vivo immersion optical clearing of human skin under the action of two different optical clearing agents (OCAs), such as an aqueous sucrose solution and a radiographic contrast agent Omnipaque™ 300 (iohexol), were obtained with the use of OCT method. The rate of reduction of light scattering coefficient, obtained through an average...

Terahertz (THz) technology offers a variety of applications in medical spectroscopy and imaging. In such applications, tissues are commonly assumed to be optically homogeneous at the THz-wavelength scale, while the THz-wave–tissue interactions are described (in a simplified manner) within the effective medium theory (EMT). Meanwhile, recent studies...

Composite nanoparticles with a gold core enveloped by cobalt ferrite nanoparticles show potential for enhanced photothermal therapy. Determining the optimal gold-to-cobalt ferrite nanoparticle ratio, dependent on size, is vital for improving treatment efficiency. We address the urgent need for advancing photothermal therapy through utilising combin...

Presents corrections to the article “Enhanced Ultraviolet Spectroscopy by Optical Clearing for Biomedical Applications”.

(1) Background: The use of electronic cigarettes has become widespread in recent years. The use of e-cigarettes leads to milder pathological conditions compared to traditional cigarette smoking. Nevertheless, e-liquid vaping can cause morphological changes in lung tissue, which affects and impairs gas exchange. This work studied the changes in morp...

Ex vivo porcine lung immersed in e‐liquid was investigated in‐depth using confocal Raman micro‐spectroscopy to assess the e‐liquid influence on the lung. It was found that lung‐related Raman band intensities at 1002, 1548, 1618 and 1655 cm ⁻¹ increased after first and second treatments except the surface, which was attributed to the well‐known opti...

Abstract Terahertz (THz) technology offers a variety of applications in label-free medical diagnosis and therapy, majority of which rely on the effective medium theory that assumes biological tissues to be optically isotropic and homogeneous at the scale posed by the THz wavelengths. Meanwhile, most recent research discovered mesoscale ( $$\sim \la...

The paper is devoted to an original method of time-resolved fluorescence molecular tomography based on asymptotic approximation to the fluorescence source function. It analyzes results the authors have obtained during last 4 years in their testing the method in numerical and physical experiments. The method is inferred to be quite promising and dir...

The paper describes a TurbidMC code that implements a perturbative Monte Carlo method to model temporal point spread functions and sensitivity functions for time-resolved fluorescence molecular tomography (FMT). The code is aimed to work with a particular FMT method published earlier (Ref. [22]) which defines the specificity of sensitivity function...

The rapid and accurate diagnosis of cancer is an important topic in clinical medicine. In the present work, an innovative method based on laser-induced breakdown spectroscopy (LIBS) combined with machine learning was developed to distinguish and classify different tumor cell lines. The LIBS spectra of cells were first acquired. Then, the spectral p...

In the original publication [...].

Photoacoustic flow cytometry is one of the most effective approaches to detect "alien" objects in the bloodstream, including circulating tumor cells, blood clots, parasites, and emboli. However, the possibility of detecting high-amplitude signals from these objects against the background of blood depends on the parameters of the laser pulse. So, th...

In this work, the change in the optical properties of the gingival mucosa in the range of 200-800 nm under the influence of liquids for electronic cigarettes (e-liquids) was studied by the method of diffuse reflectance spectroscopy. The formation of three dynamic windows of gingival optical transparency in the ultraviolet (UV) was revealed. The dif...

Background and Objectives: The reproductive system of women is a subject of diverse multidisciplinary research worldwide. These are oncological diseases, infertility of an unspecified nature, ovarian cryopreservation to preserve fertility, improvement of early diagnosis of diseases, etc. Elucidation of the mechanisms of diffusion of tissue water an...

Significance: The clinical use of optical methods for in vivo skin imaging is limited by skin strong scattering properties, which reduce image contrast and probing depth. The efficiency of optical methods can be improved by optical clearing (OC). However, for the use of OC agents (OCAs) in a clinical setting, compliance with acceptable non-toxic c...

Terahertz (THz) technology offers novel opportunities in biology and medicine, thanks to the unique features of THz-wave interactions with tissues and cells. Among them, we particularly notice strong sensitivity of THz waves to the tissue water, as a medium for biochemical reactions and a main endogenous marker for THz spectroscopy and imaging. Tis...

In this paper, the use of diffuse reflectance (Rd) spectroscopy is explored to evaluate the diffusion properties of water and sucrose in skeletal muscle during optical clearing treatments. Treating muscle samples with sucrose-water solutions with different osmolarities, collimated transmittance (Tc) and Rd measurements were performed to obtain the...

This paper describes a sapphire cryoprobe as a promising solution of the significant problem of modern cryosurgery that is the monitoring of tissue freezing. This probe consists of a sapphire rod manufactured by the edge‐defined film‐fed growth technique from Al2O3 melt and optical fibers accommodated inside the rod and connected to the source and...

The method of immersion optical clearing reduces light scattering in tissues, which improves the use of optical technologies in the practice of clinicians. In this work, we studied the optical and molecular diffusion properties of cat ovarian tissues in the follicular (F-ph) and luteal (L-ph) phases under the influence of glycerol using reflectance...

Adipose tissue (AT) optical properties for physiological temperatures and in vivo conditions are still insufficiently studied. The AT is composed mainly of packed cells close to spherical shape. It is a possible reason that AT demonstrates a very complicated spatial structure of reflected or transmitted light. It was shown with a cellular tissue ph...

This chapter describes the logic of the development of imaging systems based on X-ray radiation and the prospects for the use of nanomaterials in "cold" current sources of X-ray tubes to create systems of tomographic sensors for biomedical applications. An important direction in the development of X-ray computed tomography sensors in systems with i...

In this chapter, fiber-optic sensors integrated with optical microspheres have been presented. Fiber-optic microspheres are microscopic light spheres widely used in research and various kinds of industries. With the development of optical fiber technology in recent years, they are being increasingly used in the construction of fiber-optic sensors....

A distinctive feature of this review is a critical analysis of methods and results of measurements of the optical properties of tissues in a wide spectral range from deep UV to terahertz waves. Much attention is paid to measurements of the refractive index of biological tissues and liquids, the knowledge of which is necessary for the effective appl...

Significance: Fluorescence molecular lifetime tomography (FMLT) plays an increasingly important role in experimental oncology. The article presents and experimentally verifies an original method of mesoscopic time domain FMLT, based on an asymptotic approximation to the fluorescence source function, which is valid for early arriving photons. Aim:...

Significance: Fluorescence molecular lifetime tomography (FMLT) plays an increasingly important role in experimental oncology. The article presents and experimentally verifies an original method of mesoscopic time domain FMLT, based on an asymptotic approximation to the fluorescence source function, which is valid for early arriving photons. Aim: T...

Despite great advances in the treatment of cancer, the effectiveness of standard chemotherapy and radiation therapy for some malignant neoplasms remains insufficient. Plasmonic photothermal therapy (PPT) with plasmon resonance gold nanoparticles has been an intensively researched area in therapy in recent years [1, 2]. Successful PPT with nanoparti...

A fast calculation method was used to obtain the spectral optical properties of human normal and pathological (chromophobe renal cell carcinoma) kidney tissues. Using total transmittance, total reflectance and collimated transmittance spectra acquired from ex vivo kidney samples, the spectral optical properties of both tissues, namely the absorptio...

In this paper, the measurement and modeling of optical properties in the terahertz (THz) range of adipose tissue and its components with temperature changes were performed. Spectral measurements were made in the frequency range 0.25–1 THz. The structural models of main triglycerides of fatty acids are constructed using the B3LYP/6-31G(d) method and...

Dura mater (DM) is a connective tissue with dense collagen, which is a protective membrane surrounding the human brain. The optical clearing (OC) method was used to make DM more transparent, thereby allowing to increase in-depth investigation by confocal Raman micro-spectroscopy and estimate the diffusivity of 50% glycerol and water migration. Glyc...

The spatial resolution of an optical fiber bundle is limited by the size of a mode propagating in an individual fiber, which can be as small as a single wavelength approximately λ for conventional low-to-medium refractive-index optical fibers. High-refractive-index optical fiber bundles have a potential for the subwavelength-resolution imaging due...

A promising approach to targeted drug delivery is the remote control of magnetically sensitive objects using an external magnetic field source. This method can assist in the accumulation of magnetic carriers in the affected area for local drug delivery, thus providing magnetic nanoparticles for MRI contrast and magnetic hyperthermia, as well as the...

The recent increasing interest in the application of radiology contrasting agents to create transparency in biological tissues implies that the diffusion properties of those agents need evaluation. The comparison of those properties with the ones obtained for other optical clearing agents allows to perform an optimized agent selection to create opt...