Valeriy Tumanskiy

• Professor at Zaporizhia State Medical and Pharmaceutical University

В останні два десятиріччя, на підставі молекулярних досліджень, запропоновано більше 15 типів загибелі клітин у ссавців і вищих еукаріот.

The study was aimed at IHC study of Nestin, CD44, Ki-67, GS, AQP4 expression in the subventricular zone (SVZ) of the lateral ventricle and hippocampal formation of deceased patients with liver cirrhosis (LC) depending on the age and Child-Pugh score. The brains of 90 deceased patients aged 65 ± 3 years with non-alcoholic LC Child-Pugh class A, B an...

Sepsis-associated liver injury (SALI) induces secondary hepatotoxic brain damage, complementing the mechanisms of sepsis-associated encephalopathy. In these conditions, astrocytes play one of the central roles as being the main homeostatic glia and key cells to metabolize ammonia in the brain. The aim of the study was to determine the ammonia level...

The aim of the study – comparative analysis of the ammonia level and Alzheimer type 2 astrocytes in the brain cortex and white matter of cerebral hemispheres, hippocampus, thalamus, striatum and cerebellum in the deceased patients with liver cirrhosis of classes A, B and C according to Child–Pugh. Materials and methods. The study was performed on...

Purpose - correlation analysis of the postmortem ammonia level and numbers of Alzheimer type 2 astrocytes in different parts of the brain with intravital laboratory blood parameters of deceased patients with liver cirrhosis of varying severity. Material and methods. Autopsied brains of 90 patients with non-alcoholic liver cirrhosis (LC) of class A,...

The aim of the study – determining the immunohistochemical levels of the GFAP, GS and AQP4 in different regions of the human brain in the conditions of liver cirrhosis of different degree. Materials and methods. The study was performed on sectional material of 90 patients who suffered during lifetime from liver non-alcoholic cirrhosis of classes A...

The study aimed to determine aquaporin-4 expression in different brain regions was performed in Wistar rats subjected to cecal ligation and puncture (CLP) septic model. The immunohistochemical study of aquaporin-4 was carried out in the sensorimotor cortex, white matter, hippocampus, thalamus and caudate nucleus/putamen regions between 20 and 38 h...

Severe sepsis is accompanied by multiple organ dysfunction where acute liver failure (ALF) plays one of the most critical roles. The principal sign of acute hepatic encephalopathy accompanying ALF is ammonia induced edema of astrocytes. In case of ALF in sepsis, one can suppose increase in systemic and brain ammonia. Astroglia are target cells for...

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive types of cancer and chemotherapy resistant cancer, in the development of which poorly studied cancer stem cells (CSC) and mesenchymal stem cells (MSC) are of great importance. The purpose of the work is the analysis of the current literature data on the role of stem cells in the...

CDH1, CTNNB1 та експресії кодованих ними молекул Е-кадгерину, β-катеніну на I, II, III, IV стадіях розвитку колоректальної аденокарциноми М. А. Шишкін, В. О. Туманський Запорізький державний медичний університет, Україна Мета роботи-аналіз рівнів експресії мРНК генів CDH1, CTNNB1 і кодованих ними молекул Е-кадгерину, β-катеніну та їхніх зв'язків із...

Acute liver failure as well as acute-on-chronic liver failure result in the development of acute hepatic encephalopathy (HE) characterized by the major pathophysiologic event in form of the brain edema formation. Among brain cells, astrocytes are considered to be a central cellular population most sensitive to ammonia exposure in HE being the prima...

Pathophysiology of sepsis-associated encephalopathy (SAE) is linked to blood-brain barrier breakdown, neuroinflammation and neurotransmitter imbalance in the brain. Astroglia, the most abundant cell population within the brain, plays the critical role in control of all kinds of homeostatic processes, thereby regulating the adaptive reactions of the...

Pathophysiology of acute hepatic encephalopathy (AHE) is complex and fine links of its development are still to be recognized. It is believed that AHE mechanisms are commonly focused on the ammonia neurotoxicity associated with neuroinflammation, neurotransmitter imbalance and severe astrocytic swelling leading to generalized brain edema. Astroglia...

The aim of the study was to determine the immunohistochemical level of glutamine synthetase (GS) expression in different brain regions in the conditions of experimental acute liver failure in rats. Materials and methods. The study was conducted in Wistar rats: 5 sham (control) animals and 10 rats with acetaminophen induced liver failure model (AIL...

The aim of the study was to determine microscopic, immunohistochemical, electron microscopic, morphometric parameters of capillaries in the cerebral cortex in deceased patients with type 2 diabetes mellitus (DM) in comparison with the conditional control group and the group of deceased patients with dyscirculatory ischemic encephalopathy. Materials...

Aim – to analyze CD44 and ALDH1 immunohistochemical expression levels, as well as their correlations with proliferative and apoptotic activity of cancer cells in colorectal adenocarcinoma (CRA) of stages I–IV (pTNM). Materials and methods. Pathohistological and immunohistochemical studies of surgical material from 30 patients who underwent surgica...

Since the beginning of the twentieth century, in parallel with the standards of the whole brain death and the brain stem death of a donor used in transplantology, the concept of donation after human circulatory death has received a practical revival. Aim: analysis of the revival of the concept of circulatory death of a person and donor protocols af...

The criteria for brain death (BD), the parameters of their clinical and pathomorphological determination and use in organ harvesting for transplantation are being clarified in different countries to date. Objective: analysis of the formation and clinical and pathological characteristics of brain death. The review provides retrospective analysis o...

The criteria for brain death (BD), the parameters of their clinical and pathomorphological determination and use in organ harvesting for transplantation are being clarified in different countries to date. Aim: analysis of the formation and clinical and pathological characteristics of brain death. The review provides retrospective analysis of the fo...

Мета роботи — охарактеризувати можливості патоморфологічного визначення в трепанобіоптатах печінки активності хронічного стеатогепатиту різного генезу і спрямованості репаративних процесів. Гістологічними, гістохімічними й імуногістохімічними методами досліджена печінка 160 хворих на алкогольний стеатогепатит (АСГ), 123 хворих на неалкогольний стеа...

Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive types of cancer and chemotherapy resistant cancer, in the development of which poorly studied cancer stem cells (CSC) and mesenchymal stem cells (MSC) are of great importance. The purpose of the work is the analysis of the current literature data on the role of stem cells in the...

Colorectal adenocarcinoma (CRA) is the result of numerous mutations accumulation. The aim of the work was to study KRAS gene transcriptional activity at I, II, III, and IV stages of CRA development and to analyze the correlations between KRAS and Ki-67, TP53, CDH1, CTNNB1 genes transcriptional activity. Pathohistological and molecular-genetic study...

Aim – analysis of CDH1 and CTNNB1 genes mRNA expression levels and expression levels of E-cadherin, β-catenin proteins coded by these genes, and also their correlations with KRAS gene transcriptional activity level on the I, II, III, IV stages (pTNM) of colorectal adenocarcinoma (CRA) development. Materials and methods. Parallel immunohistochemica...

Изобретение относится к медицине, клинико-аналитической химии, судебно-химической экспертизе, патоморфологии и гепатологии. Заявленный способ хромато-масс-спектрометрического определения холестерина, стеариновой и пальмитиновой кислот в биологическом материале путем проведения экстракции, которую осуществляют путем обработки гомогенизированного обр...

Until recently, a discussion about the mechanisms of development and the biological role of the ductular reaction, which develops in patients with chronic liver diseases continues among hepatologists and pathomorphologists. Purpose of the study. To characterize the pathomorphological features and significance of the ductular response in liver cirrh...

Until recently, a discussion about the mechanisms of development and the biological role of the ductular reaction, which develops in patients with chronic liver diseases continues among hepatologists and pathomorphologists. Purpose of the study. To characterize the pathomorphological features and significance of the ductular response in liver cirr...

Until recently, among the pathologists, hepatologists and gastroenterologists, a discussion continues on the morphogenesis and gradation of liver fibrosis in non-alcoholic patients (NASH) and alcoholic steatohepatitis (ASH). Purpose of the study. Studying the main types and sources of liver fibrosis in patients with nonalcoholic and alcoholic steat...

Until recently, among the pathologists, hepatologists and gastroenterologists, a discussion continues on the morphogenesis and gradation of liver fibrosis in non-alcoholic patients (NASH) and alcoholic steatohepatitis (ASH). Purpose of the study. Studying the main types and sources of liver fibrosis in patients with nonalcoholic and alcoholic steat...

Aim – to study the features of MUC1, MUC2, MUC5AC, Cdx-2 immunohistochemical expression in hyperplastic polyps and intestinal-type gastric adenocarcinoma, and their relationships with the proliferation and apoptosis markers expression levels, EGFR and HER-2/neu growth receptors. Materials and мethods. Pathomorphological and immunohistochemical stu...

Squamous cervical cancer (SCC) is a common tumor in women, which is preceded by the series of pathological processes, among which the key role is played by cervical intraepithelial neoplasia (CIN). Aim. To study the characteristics of immunohistochemical (IHC) expression of Ki-67, p16INK4a, HPV16 in squamous cervical epithelium (SCE) with dysplast...

Aim – to study the features of expression of the K-RAS gene mRNA and the ErbB family growth receptors (EGFR and HER-2/neu) by the tumor cells of the intestinal-type gastric adenocarcinoma, and also their relations to the processes of cell proliferation and apoptosis. Materials and methods. Pathohistological, immunohistochemical and molecular-genet...

Background. The process of extracellular matrix degradation, provided by the matrix metalloproteinases system, plays an important role in cancerogenesis. Objective. To study the features of immunohistochemical expression of MMP-2, TIMP-1 by hyperplastic polyps’ cells and intestinal-type gastric adenocarcinoma cells. Methods. Pathohistological and i...

Molecular genetics abnormalities, underlying aberrant expression of E-cadherin and β-catenin in malignant tumors, are still unclear. Aim: to compare the mRNA expression levels of CDH1 and CTNNB1 genes and the immunohistochemical expression levels of E-cadherin and β-catenin proteins in intestinal-type gastric adenocarcinoma. Materials and methods...

It is known that higher saturated fatty acids (HSFA) having the lipotoxicity, play an important role in the development of non-alcoholic steatohepatitis (NASH), however, the results of research of cholesterol and HSFA in the liver with NASH of varying severity, as obtained by high performance liquid chromatography-mass spectrometry (HPLC-MS), have...

54) СПОСІБ ВИЗНАЧЕННЯ СТУПЕНЯ СТЕАТОЗУ В БІОПТАТАХ ПЕЧІНКИ (57) Реферат: Спосіб визначення ступеня стеатозу в біоптатах печінки, шляхом проведення патоморфологічного дослідження з подальшим морфометричним визначенням поширеності стеатозу. За допомогою програми ImageJ здійснюють сегментацію зображення та переведення його в двокольорове (бінарне), ро...

The violation of Wnt-signaling pathway and cyclic neoangiogenesis is early events in endometrial carcinogenesis. In this process an important role is played by epigenetic modifications and mutations of CDH1, CTNNB1, VEGFA genes, followed by expression violations of E-cadherin, β-catenin, VEGFA encoded by them. The aim. To study the expression leve...

Aim. Comparison analysis of expression levels of Ki-67, p53, and caspase 3 was performed between the tumor cells and the stromal cells of non-invasive intestinal-type gastric adenocarcinoma on the one hand and the tumor cells and the stromal cells of invasive intestinal-type gastric adenocarcinoma on the other hand. Materials and methods. For this...

Background. Endometrial cancer occupies the sixth place among newly diagnosed neoplasm in women each year. Objective. Immunohistochemical characterization of the expression of the estrogen receptors-α, progesterone receptors, p16, p53, Ki-67, caspase-3 by the cells of invasive endometrioid endometrial adenocarcinomas of various grade. Methods. The...

Background. According to global statistics prostate cancer (РС) is the second leading cause of death and takes the second place in males cancer structure. Objective. Trephine biopsies of 32 patients with acinar РС and 30 patients with benign prostatic hyperplasia (BPH), which in 22 patients were combined with multifocal prostatic intraepithelial ne...

54) СПОСІБ ВИЯВЛЕННЯ ДВОВАЛЕНТНОГО ЗАЛІЗА В БІОПСІЙНОМУ МАТЕРІАЛІ (57) Реферат: Спосіб виявлення двовалентного заліза в біопсійному матеріалі шляхом фіксації тканини в 10 % формаліні, забуференому до рН 7,0, основного забарвлення з використанням розчину батофенантроліну у 3 % оцтовій кислоті протягом 2 годин, стабілізації заліза в біоптаті, подальш...

Endometrial cancer occupies the sixth place among newly diagnosed neoplasms in women. In late stage it has unpredictable prognosis. Immunohistichemical studies of the tumor are very important for prognosis of tumor course. The aim. To characterize the expression of the estrogen receptors-alpha, progesterone receptors, p53, p16, ki-67 and caspase 3...

Aim. The article describes morphological characteristics of the heterovalent iron compounds (ferric (Fe3 +) iron, ferrous (Fe2 +) iron) deposition in the acini of the liver. Methods and results. Using histochemical methods, features of accumulation of ferrous (Fe2 +) and ferric (Fe3 +) iron in hepatic acinar zone were studied in the trephine biopsy...

Aim. In puncture trephine biopsy of the liver differential immunohistochemical (IHC) parameters of hepatocellular carcinoma (HCC) in 30 patients, cholangiocellular cancer (CC) of the liver in 15 patients, and liver metastases of malignant tumors of solid, trabecular structure [melanoma (12), breast cancer (10), neuroendocrine tumors (12), prostate...

Aim. Differential immunohistochemical parameters of primary liver cancer and liver metastases of ductal pancreatic cancer (DPC) were studied in puncture trephine biopsy of the liver of 23 patients with hepatocellular carcinoma (HCC), 2 patients combined hepato-cholangiocellular cancer (CHCC), 15 patients cholangiocellular cancer (CCC) and 12 patien...

In Ukraine 60,9% of patients with gastric cancer die within 1 year after ascertainment of diagnosis. Aim: In order to investigate the effect of Ki-67 overexpression on life expectancy, study was conducted among 129 patients with gastric cancer of 0 - III stages, who received treatment in Zaporizhzhia Regional Clinical Oncology Center from June 200...

Differential diagnosis between pancreatic ductal adenocarcinoma and chronic pancreatitis with severe fibrosis of the stroma is often complicated by the fact that the rich desmoplastic tumor stroma imitates diffuse foci of fibrosis in pancreatitis. Aim. In order to quantify the expression of stromal markers in desmoplastic stroma of pancreatic duct...

Aim. In order to determine E-cadherin and β-catenin expression levels in the invasive endometrioid endometrial carcinoma (EEC) cells and areas of the immunopositive cells in the center of the tumor and in the area of tumor invasion into the myometrium a pathohistological and immunohistochemical study of histological specimens of operative material...

Aim. Histopathological and immunohistochemical study of trephine biopsy of the liver in 94 patients, including 55 patients who suffered from hepatocellular carcinoma (HCC) and 39 - cholangiocellular carcinoma (CCC) with various sizes determined by ultrasound of the liver were carried out. Methods and results. The photo digital morphometry was used...

Aim. In order to determine the expression level of immunohistochemical markers of matrix metalloproteinase-9 (ММР-9) and its tissue inhibitor TIMP-1, and area of immunopositive cells а comprehensive histopathological, histological and immunohistochemical study of liver biopsy specimens was carried out in 94 patients, of which 55 (58.51%) patients h...

To optimize educational and research environment for philosophy doctors the following solutions were realized in Zaporozhye State Medical University: • Involvement of youth to scientific research since undergraduate school. • Creation of the appropriate environment for talented undergraduate and postgraduate students who are interested in science....

Aim. In order to research proliferation and apoptosis immunohistochemical study of expression of Ki-67 and Caspase-3 inbiopsies of 42 gastric hyperplastic polyps was conducted. Methods and results. It was found that gastric hyperplastic polyps are characterized by middle expression level of Ki-67 (2,4±0,09 points) and extremely low expression leve...

Aim. In immunohistochemical research of biopsy and postoperative material of 120 patients with the pancreatic ductal adenocarcinoma the levels of growth receptors of ErbB family and cancer prognostic markers expression were determined. Methods and results. Positive expression of EGFR by the tumour cells is determined in 87,5% of patients with duct...

Aim. Expression level of immunohistochemical markers such as HepPar-1, AFP, CK7, CK20 and the area of immunopositive cells in cholangiocellular liver cancer, and their differences from hepatocellular carcinoma Methods and results. Histopathological, histochemical and immunohistochemical research of trephine was determined in the liver in 90 patien...

Aim. Immunohistochemical determination of cell proliferation and apoptosis processes in PanIN in ductal adenocarcinoma (DA) and chronic pancreatitis (CP). Materials and methods. Comprehensive histological, histochemical and immunohistochemical (IHC) study was carried out on the biopsy-postoperative material of 36 patients with CP with severe fi bro...

Introduction. The development of tumors occurs as a multistage process, where each step corresponds to certain genetic changes of cells and it is a reflection characteristic of different histological forms of cancer. This usually multiple changes include the expression of several oncogenes and inactivation of two or more suppressor genes. Such even...

V. A. Tumanskiy, M. D. Zubko Aims. In order to determine the microscopic features and expression level of HepРar-1, AFP, CK7, CK20 by the cells of hepatocellular carcinoma histopathological, histochemical and immunohistochemical study of 53 liver biopsies from patients with hepatocellular carcinoma was conducted. Methods and results. The photo digi...

The research of pathomorphological features of liver fibrosis in patients with chronic viral hepatitis C associated with hepatic steatosis has shown that as opposed to cases of perisinusoidal-central-lobular and portal-septal fibrosis, typical for chronic viral hepatitis C without steatosis, in patients with chronic viral hepatitis C with steatosis...