Valerie M Williamson

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Verified

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• Doctor of Philosophy
• Emeritus professor at University of California, Davis

Root-knot nematodes (Meloidogyne spp.) are among the most destructive agricultural pests that cause significant yield losses across a wide range of crops. Meloidogyne hapla, a diploid species, is a valuable model for studying root-knot nematodes due to its parasitic diversity, small genome, and a reproductive strategy that facilitates genetic analy...

We present the chromosome-scale genome assembly of the allopolyploid root-knot nematode Meloidogyne javanica. We show that the M. javanica genome is predominantly allotetraploid, comprising two subgenomes, A and B, that most likely originated from hybridisation of two ancestral parental species. The assembly was annotated using full-length non-chim...

Root-knot nematodes (Meloidogyne spp.) are highly evolved obligate parasites threatening global food security. These parasites have a remarkable ability to establish elaborate feeding sites in roots, which are their only source of nutrients throughout their life cycle. A wide range of nematode effectors have been implicated in modulation of host pa...

Describing the genome assembly of Meloidogyne javanica. The assembly is chromosome-scale and has been phased into subgenomes for the first time. Synteny analysis reveals high amounts of conserved synteny, and suggestd several chromosomal fission events exclusive to one subgenome.

We present the chromosome-scale genome of the allopolyploid root-knot nematode Meloidogyne javanica . We show that the M. javanica genome is predominantly allotetraploid, comprising two subgenomes, A and B, that most likely originated from hybridisation of two ancestral parental species. The assembly is annotated using full-length non-chimeric tran...

Root-knot nematodes ( Meloidogyne spp.) are highly evolved obligate parasites that threaten global food security. These parasites have a remarkable ability to establish elaborate feeding sites in roots, which are their only source of nutrients throughout their life cycle. A wide range of nematode effectors have been implicated in modulation of host...

Plant-parasitic nematodes spend much of their lives inside or in contact with host tissue and molecular interactions constantly occur and shape the outcome of parasitism. Eggs of these parasites generally hatch in the soil, and the juveniles must locate and infect an appropriate host before their stored energy is exhausted. Components of host exuda...

Plant-parasitic nematodes are a continuing threat to food security, causing an estimated 100 billion USD in crop losses each year. The most problematic are the obligate sedentary endoparasites (primarily root knot nematodes and cyst nematodes). Progress in understanding their biology is held back by a lack of tools for functional genetics: forward...

Background and AimsPlant-parasitic nematodes are able to sense and respond to gradients of chemical signals. How pH and inorganic salts in the rhizosphere affect nematode accumulation and host-seeking is poorly understood. We investigate the response of different groups of plant-parasitic nematodes to pH and salt concentration gradients.Methods Res...

Plant-parasitic nematodes are a continuing threat to food security, causing an estimated 100 billion USD in crop losses each year. The most problematic are the obligate sedentary endoparasites (primarily root knot nematodes and cyst nematodes). Progress in understanding their biology is held back by a lack of tools for functional genetics: forward...

Second stage juveniles (J2s) of plant-parasitic nematodes hatch from eggs into the soil and localize to the roots of host plants. The success of these non-feeding J2s depends on their ability to locate and infect a suitable host. The attraction of plant parasitic nematodes to the host root has been a subject of study for the past 80 years following...

Microorganisms and nematodes in the rhizosphere profoundly impact plant health, and small-molecule signaling is presumed to play a central role in plant rhizosphere interactions. However, the nature of the signals and underlying mechanisms are poorly understood. Here we show that the ascaroside ascr#18, a pheromone secreted by plant-parasitic nemat...

The cover image, by Daniel F. Klessig et al., is based on the Original Article Nematode ascaroside enhances resistance in a broad spectrum of plant–pathogen systems. DOI: 10.1111/jph.12795. Photo credit: Cover image © Aardra Kachroo University of Kentucky Images

Recognition of specific molecule signatures of microbes, including pathogens, induces innate immune responses in plants, as well as in animals. Analogously, a nematode pheromone, the ascaroside ascr#18, induces hallmark plant defences including activation of (a) mitogen‐activated protein kinases, (b) salicylic acid‐ and jasmonic acid‐mediated defen...

Nematode chemosensation is a vital component of their host-seeking behaviour. The globally important phytonematode, Meloidogyne incognita, perceives and responds (via their sensory organs such as amphids and phasmids) differentially to various chemical cues emanating from the rhizosphere during the course of host finding. However, compared to the f...

Root-knot nematodes (RKN; Meloidogyne spp.) can parasitize over 2,000 plant species and are generally considered to be the most agriculturally damaging group of plant-parasitic nematodes worldwide. Infective juveniles (J2) are non-feeding and must locate and invade a host before their reserves are depleted. However, what attracts J2 to appropriate...

The Root-Knot Nematodes (RKN; genus Meloidogyne) are important plant parasites causing substantial agricultural losses. The Meloidogyne incognita group (MIG) of species, most of which are obligatory apomicts (mitotic parthenogens), are extremely polyphagous and important problems for global agriculture. While understanding the genomic basis for the...

Interactions between species are pervasive among plants, animals, and microbes, and identifying the molecular signals involved is an active area of research.. Organisms engage in extensive cross-species molecular dialog, yet the underlying molecular actors are known for only a few interactions. Many techniques have been designed to uncover genes in...

The Root-Knot Nematodes (RKN; genus Meloidogyne) are important plant parasites causing substantial agricultural losses. The Meloidogyne incognita group (MIG) of species, most of which are obligatory apomicts (mitotic parthenogens), are extremely polyphagous and important problems for global agriculture. While understanding the genomic basis for the...

Nematodes and other organisms perceive and respond to plant root exudates. These exudates are affected by the condition and genetic makeup of the plant. Attraction of the root-knot nematode Meloidogyne hapla to roots is altered in plants with mutations affecting ethylene signaling, suggesting that the root exudates to which the nematode responds ar...

Plant parasitic nematodes respond to root exudates to locate their host roots. In our studies second stage juveniles of Heterodera glycines, the soybean cyst nematode (SCN), quickly migrated to soybean roots in Pluronic F-127 gel. Roots of soybean and non-host Arabidopsis treated with the ethylene (ET)-synthesis inhibitor aminoethoxyvinylglycine (A...

Plant-defense responses are triggered by perception of conserved microbe-associated molecular patterns (MAMPs), for example, flagellin or peptidoglycan. However, it remained unknown whether plants can detect conserved molecular patterns derived from plant-parasitic animals, including nematodes. Here we show that several genera of plant-parasitic ne...

Meloidogyne spp. (root-knot nematodes) are the most damaging plant-parasitic nematodes to commercial agriculture. Currently used nematicides are of limited effectiveness as well as toxic to other organisms. The present study examines whether disruption of cyclic nucleotide signaling pathways using phosphodiesterase (PDE) inhibitors could serve as t...

The growing portfolio of sequenced plant-parasitic nematodes genomes is helping to drive the research agenda for the discipline of plant nematology. Comparative genomics has confirmed much of what had been deduced from expressed sequence tag sequencing, and expanded our understanding of the extent of horizontal gene transfer as a source of novelty...

The asexual root-knot nematodes (Meloidogyne spp; RKNs) exemplified by M. incognita are widespread and damaging pests in tropical and sub-tropical regions worldwide. Comparison of amplification products of two adjacent polymorphic regions of the mitochondrial genome using DNA extracts of characterized RKN strains including 15 different species indi...

The yam nematode, Scutellonema bradys, which can cause dry rot disease of yam (Dioscorea spp.), was recorded for the first time from Costa Rica in four species of yam occurring in the Atlantic and north regions. Morphometric measurements from two populations from each region using ten female and 11 male characters corresponded with previous descrip...

The root-knot nematode Meloidogyne hapla can reproduce on a wide range of crop species, but there is variability in host range and pathogenicity both within and between isolates. The inbred strain VW9 causes galling but does not reproduce on Solanum bulbocastanum clone SB22 whereas strain VW8 causes little galling and reproduces poorly on this host...

Root knot nematodes (RKN; Meloidogyne spp.) cause severe losses worldwide to a wide range of crops. Crop rotations with resistant hosts can be used to control losses, but the wide host range of RKN limits this option. In this study, we found that the wheat cultivar Lassik is resistant to several isolates of the RKN species M. incognita and M. javan...

Nine Nigerian populations of Scutellonema bradys, obtained from infected yam (Dioscorea spp.), were assessed following inoculation onto susceptible yam (Dioscorea rotundata) cv. TDr131 in pot and field experiments in Nigeria between 2002 and 2004. In addition, geographically separated populations, two each from Benin, Burkina Faso, Côte d'Ivoire, G...

The presence of Meloidogyne enterolobii on two hosts and in several locations in Costa Rica is reported. Meloidogyne spp. females were extracted from root of acerola and of wild and introduced guava and their perineal patterns were examined. Females from acerola showed round or dorso-ventrally ovoid perineal patterns, in some cases with lateral lin...

Root-knot nematodes (Meloidogyne spp.) cause major yield losses to many of the world's crops, but efforts to understand how these pests recognize and interact with their hosts have been hampered by a lack of genetic resources. Starting with progeny of a cross between inbred strains (VW8 and VW9) of Meloidogyne hapla that differed in host range and...

Infective juveniles of the root-knot nematode Meloidogyne hapla are attracted to the zone of elongation of roots where they invade the host, but little is known about what directs the nematode to this region of the root. We found that Arabidopsis roots exposed to an ethylene-synthesis inhibitor attracted significantly more nematodes than control ro...

Three closely related parthenogenetic species of root-knot nematodes, collectively termed the Meloidogyne incognita-group, are economically significant pathogens of diverse crop species. Remarkably, these asexual root-knot nematodes are capable of acquiring heritable changes in virulence even though they lack sexual reproduction and meiotic recombi...

Research on nematode parasitism tackles fundamental questions in plant development and host–parasite interaction. The plant-parasitic cyst and root-knot nematodes (RKNs) have evolved sophisticated strategies for exploiting plants with high impacts in agriculture worldwide. We review here recent knowledge acquired on putative parasitism genes and on...

Root-knot nematodes are obligate parasites of a wide range of plant species and can feed only on the cytoplasm of living plant cells. In the absence of a suitable plant host, infective juveniles of strain VW9 of the Northern root-knot nematode, Meloidogyne hapla, when dispersed in Pluronic F-127 gel, aggregate into tight, spherical clumps containin...

The nomenclature of genes in Caenorhabditis elegans is based on long-standing, successful guidelines established in the late 1970s (Horvitz et al., 1979). Over time these guidelines have matured into a comprehensive, systematic nomenclature that is easy to apply, descriptive and therefore highly informative. Recently, a flood of parasitic nematodes...

Root-knot nematodes (Meloidogyne spp.) are obligate endoparasites of a wide range of plant species. The infective stage is attracted strongly to and enters host roots at the zone of elongation, but the compounds responsible for this attraction have not been identified. We developed a simple assay to investigate nematode response to chemical gradien...

Pluronic F-127 is a stable, non-toxic, copolymer that forms a gel at room temperature and a liquid at 15°C when the concentration is 20-30%. Root-knot nematode (Meloidogyne spp.) second-stage juveniles can move freely through the gel and display attraction toward roots of tomato, Medicago truncatula, common bean and Arabidopsis. The excellent clari...

Plant-parasitic nematodes are the most destructive group of plant pathogens worldwide and are extremely challenging to control. The recent completion of two root-knot nematode genomes opens the way for a comparative genomics approach to elucidate the success of these parasites. Sequencing revealed that Meloidogyne hapla, a diploid that reproduces b...

We have established Meloidogyne hapla as a tractable model plant-parasitic nematode amenable to forward and reverse genetics, and we present a complete genome sequence. At 54 Mbp, M. hapla represents not only the smallest nematode genome yet completed, but also the smallest metazoan, and defines a platform to elucidate mechanisms of parasitism by w...

Specific host-parasite interactions exist between species and strains of plant parasitic root-knot nematodes and the Gram-positive bacterial hyperparasite Pasteuria penetrans. This bacterium produces endospores that adhere to the cuticle of migrating juveniles, germinate and colonise the developing female within roots. Endospore attachment of P. pe...

The Mi-1 gene in tomato confers effective resistance against several species of root-knot nematode, including Meloidogyne javanica. A strain of M. javanica that can reproduce on tomato with Mi-1 was obtained from a culture of an avirulent strain after greenhouse selection. DNA blots and amplified fragment length polymorphism (AFLP) analysis indicat...

In the study of biological systems, often the amount of starting material available for molecular analysis is limiting. In some situations, only a few cells in a tissue are expressing genes of interest or the tissue is in limited supply. A cDNA library from the targeted cells is preferable to a library constructed from the entire tissue containing...

Many isolates of the plant-parasitic nematode Meloidogyne hapla reproduce by facultative meiotic parthenogenesis. Sexual crosses can occur, but, in the absence of males, the diploid state appears to be restored by reuniting sister chromosomes of a single meiosis. We have crossed inbred strains of M. hapla that differ in DNA markers and produced hyb...

Many plant disease resistance genes (R-genes) encode proteins characterized by the presence of a nucleotide-binding site (NBS) and a leucine-rich repeat (LRR) region and occur in clusters of related genes in plant genomes. One such gene, Mi-1, confers isolate-specific resistance against root-knot nematodes, aphids and whiteflies in cultivated tomat...

We have developed a simple PCR assay protocol for detection of the root-knot nematode (RKN) species Meloidogyne arenaria, M. incognita, and M. javanica extracted from soil. Nematodes are extracted from soil using Baermann funnels and centrifugal flotation. The nematode-containing fraction is then digested with proteinase K, and a PCR assay is carri...

Parasitic nematodes infect thousands of plant species, but some plants harbor specific resistance genes that defend against these pests. Several nematode resistance genes have been cloned in plants, and most resemble other plant resistance genes. Nematode resistance is generally characterized by host plant cell death near or at the feeding site of...

The Mi-1.2 gene in tomato (Solanum lycopersicum) is a member of the nucleotide-binding leucine-rich repeat (NBLRR) class of plant resistance genes, and confers resistance against root-knot nematodes (Meloidogyne spp.), the potato aphid (Macrosiphum euphorbiae), and the sweet potato whitefly (Bemisia tabaci). Mi-1.2 mediates a rapid local defensive...

Five isolates of M. hapla originating from the Netherlands and California were inbred by sequential transfer of single egg masses to produce six strains. Cytological examination showed that oocytes of these strains underwent meiosis and had n = 16 chromosomes. Strains were tested for ability to infect and to develop on several hosts by in vitro ass...

The begomovirus-resistant breeding lines being developed in Guatemala were tested for the CAPS markers, REX-1 and Cor-Mi, that are linked to the Mi-1 locus for root-knot nematode resistance. Both markers gave false positive results for several breeding lines that are susceptible to M. incognita and have L. hirsutum (line Ih902) or L. chilense (line...

Currently, the only genetic resistance against root-knot nematodes in the cultivated tomato Solanum lycopersicum (Lycopersicon esculentum) is due to the gene Mi-1. Another resistance gene, Mi-3, identified in the related wild species Solanum peruvianum (Lycopersicon peruvianum) confers resistance to nematodes that are virulent on tomato lines that...

In tobacco and other Solanaceae species, the tobacco N gene confers resistance to tobacco mosaic virus (TMV), and leads to induction of standard defense and resistance responses. Here, we report the use of N-transgenic tomato to identify a fast-neutron mutant, sun1-1 (suppressor of N), that is defective in N-mediated resistance. Induction of salicy...

A PCR-based assay for identification of six species of Pratylenchus common in California is described. In this assay, five forward species-specific primers were designed from the internal variable portion of the D3 expansion region of the 26S rDNA and were each used with a single, common reverse primer. The optimized species-specific primers produc...

The gene Mi-1 confers effective resistance in tomato ( Lycopersicon esculentum) against root-knot nematodes and some isolates of potato aphid. This locus was introgressed from L. peruvianum into the corresponding region on chromosome 6 in tomato. In nematode-resistant tomato, Mi-1 and six homologs are grouped into two clusters separated by 300 kb....

Mi-1.2, a member of the intracellular, nucleotide-binding site-leucine-rich repeat family of resistance genes, confers resistance in tomato (Lycopersicon esculentum) against both root-feeding nematodes and leaf-feeding aphids. Nematode resistance is effective in all life stages of the plant; in contrast, Mi-mediated aphid resistance is developmenta...

The Mi-1 gene of tomato confers resistance against three species of root-knot nematode in tomato (Lycopersicon esculentum). Transformation of tomato carrying Mi-1 with a construct expressing NahG, which encodes salicylate hydroxylase, a bacterial enzyme that degrades salicylic acid (SA) to catechol, results in partial loss of resistance to root-kno...

The tomato gene Mi-1 confers resistance to root-knot nematodes (Meloidogyne spp.), potato aphid, and whitefly. Using genetic screens, we have isolated a mutant, rme1 (resistance to Meloidogyne spp.), compromised in resistance to M. javanica and potato aphid. Here, we show that the rme1 mutant is also compromised in resistance to M. incognita, M. ar...

The goal of this study was to assess the susceptibility of the nematode resistance gene, Mi-1.2, to transgene inactivation. The stability of Mi-mediated nematode resistance and Mi-1.2 transcripts levels was observed in two independently transformed tomato lines carrying Mi-1.2. In both lines a reduction in resistance was noted in the T2 generation,...

The complete genome sequence of the insect pathogen and nematode symbiont Photorhabdus luminescens identifies a trove of antibiotic and toxin genes.

Root-knot nematodes and cyst nematodes are obligate, biotrophic pathogens of numerous plant species. These organisms cause dramatic changes in the morphology and physiology of their hosts. The molecular characterization of induced plant genes has provided insight into the plant processes that are usurped by nematodes as they establish their special...

The tomato gene Mi-1.2 confers resistance against root-knot nematodes and some isolates of potato aphid. Resistance to the whitefly Bemisia tabaci previously has been observed in Mi-bearing commercial tomato cultivars, suggesting that Mi, or a closely linked gene, is responsible for the resistance. The response of two biotypes of B. tabaci to tomat...

The root-knot nematode resistance gene Mi from tomato encodes a nucleotide-binding/leucine-rich repeat (NB/LRR) protein with a novel amino-terminal domain compared to related disease-resistance genes. The closely linked paralog Mi-1.1, which does not confer nematode resistance, encodes a protein 91% identical to the functional copy, Mi-1.2. The chi...

Meloidogyne fallax (Karssen, 1996) has been recorded for the first time in Australia from potato tubers and soil collected in the south-east of South Australia. Identification was based on morphological observations and DNA sequencing.

Mi is a dominant locus in tomato that confers resistance to both root knot nematodes, Meloidogyne spp., and potato aphids, Macrosiphum euphorbiae (Thomas). The goal of this study was to investigate the breadth of Mi-mediated aphid resistance. To examine variability in potato aphid response, the effect of Mi on seven isolates of the potato aphid fro...

The tomato Mi gene confers resistance against root-knot nematodes and potato aphids. Chimeric constructs of the functional gene, Mi-1. 2, with a homolog, Mi-1.1, were produced, and their phenotypes were examined in Agrobacterium rhizogenes-transformed roots. Exchange of the leucine-rich repeat (LRR) region of Mi-1.1 into Mi-1.2 resulted in the loss...

The effect of the tomato (Lycopersicon esculentum Miller) resistance gene, Mi, on the feeding behavior of the potato aphid, Macrosiphum euphorbiae (Thomas), was studied using an AC electronic monitoring system. Aphids were monitored for at least 16 h on nearly isogenic tomato lines, and we observed waveforms corresponding to the stylet pathway phas...

cDNA clones of genes expressed in small amounts of material can be hard to obtain because the construction of conventional cDNA libraries requires microgram amounts of poly (A)+ RNA (1). The polymerase chain reaction (PCR), which is commonly used to amplify tiny amounts of DNA (2,3), has been adapted to facilitate the construction of cDNA libraries...