V. Montoro Angulo

V. Montoro Angulo
University of Castilla-La Mancha · Agricultural and Genetic Science and Technology

About

68
Publications
11,887
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1,323
Citations
Citations since 2017
16 Research Items
590 Citations
2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140
2017201820192020202120222023020406080100120140
Introduction

Publications

Publications (68)
Article
Multiple infections or co-exposure to pathogens should be considered systematically in wildlife to better understand the ecology and evolution of host-pathogen relationships, so as to better determine the potential use of multiple pathogens as indicators to guide health management. We describe the pattern of co-exposure to several pathogens (i.e. s...
Article
Full-text available
Human–wildlife conflicts are a growing problem in Northern Hemisphere where wild ungulates are one of the taxonomic groups most frequently involved. To mitigate these conflicts, it is essential to develop preventive actions able to avoid encounters between wildlife and human (activities). We here employed photo-trapping to evaluate the behaviour of...
Article
Full-text available
The heterogeneous nature of ejaculates highlights the relevance of studying the behavior of different sperm subpopulations. Changes in sperm motility and the increase in tyrosine phosphorylation are key events that usually occur during capacitation and can be modified by the cryopreservation process. However, the relationship between both events re...
Article
Full-text available
To date, the underlying mechanisms by which cAMP modulators act during in vitro maturation to improve oocyte developmental competence are poorly understood. Here, we sought to fill this knowledge gap by evaluating the use of phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and adenylyl cyclase activator forskolin during a culture peri...
Book
Full-text available
This book, aimed at professionals in the sector such as veterinarians, livestock farmers or wildlife managers, is the first volume of a manual that lists and details the different sources of risk of tuberculosis for cattle. In each section, the risk is described, its epidemiological role is detailed and control measures are proposed, providing a co...
Article
Bluetongue is a vector-borne disease affecting domestic and wild ruminants, with a major socioeconomic impact. Endemic circulation of the bluetongue virus serotype 4 (BTV-4) and BTV-1 have occurred in Spain since 2004 and 2007, respectively. However, epidemiological studies have seldom been approached from a long-term perspective in wild ruminants....
Article
Full-text available
The hepatitis E virus (HEV) is an emerging zoonotic pathogen whose main reservoir is suids. Most of the ecological and epidemiological aspects of its sylvatic cycle remain unknown. Thus, in this work, we study the drivers of HEV exposure in the wild boar population of Doñana National Park (DNP, southwest Spain) operating in the medium and long-term...
Chapter
The interfaces among different compartments have been spaces of transition and contact where pathogens have adapted to new hosts. The domestication and establishment of local settlements modelled multiple wildlife–livestock–human interfaces, characterized by the maintenance of “crowd diseases” (both by humans and/or by animals) and the continuous p...
Article
The effective management of shared pathogens between wild ungulates and livestock requires the understanding of the processes of interaction between them. In this work we studied the interspecific frequency of interaction (ifreq) and its spatio-temporal pattern between wild and domestic ungulates that coexist in free-ranging farms. For this purpose...
Article
Full-text available
A major limiting factor for the development of in vitro embryo production (IVP) in wild species, such as Iberian red deer, compared to livestock animals is the poor availability and limited access to biological material. Thus, the use of post-mortem ovaries from slaughtered animals represent a source of oocytes for the large scale production of emb...
Article
Full-text available
Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics”...
Article
Our aim was to optimize 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) immunodetection in order to detect DNA damage caused by oxidative stress that may not be detected by other DNA integrity analysis techniques, especially due to the high compaction of DNA in ruminants. Semen samples from 6 rams were cryopreserved. After thawing, samples were subjected to t...
Article
Full-text available
Understanding the role that facultative scavenger species may play in spreading infectious pathogens, and even becoming reservoirs for humans, domestic and wild ungulates or, on the contrary, preventing the spread of disease, requires a prior understanding of the pattern of carrion scavenging in specific scenarios. The objectives of this paper are...
Article
Full-text available
La mejora de la calidad de la leche es uno de los objetivos fundamentales en los actuales sistemas de producción del ovino lechero. La Fundación Consejo Regulador de la Denomina- ción de Origen Queso Manchego y la Asociación Nacional de Criadores de Ganado Ovino Selecto raza Manchega participan en los Proyectos de Investigación en materia de cali...
Article
Manchega sheep production is of great socio-economic importance in Castilla-La Mancha, being the milk from this breed the raw material of Protected Designation of Origin (PDO) Manchego cheese. The Breeding Program developed by the National Association of Cattle Breeders Sheep Breed Select Mancha (AGRAMA), began in 1988, being the current objective...
Article
Full-text available
Improvement of milk quality is one of the principal objectives in dairy sheep production systems. Regulator Council of PDO Manchego Cheese and National Association of Manchega Breeders take part in some milk quality research projects from Dairy Laboratory of the Regional Selection and Animal Reproduction Center, belonging to the Regional Government...
Article
Full-text available
Reactive oxygen species (ROS) are fundamental for intracellular signalling. In spermatozoa, they are involved both to apoptosis and to capacitation, and changes in ROS levels can alter the balance between these two processes. Oestrous sheep serum (OSS) is considered an efficient agent for in vitro capacitation of ram spermatozoa. We have explored t...
Article
Full-text available
The interactions of extensively farmed livestock with wild ungulates contribute to the transmission and maintenance of, among other diseases, tuberculosis (TB) at the interface in South Central Spain (SCS), where farming typically occurs close to wildlife habitats. The aim of this paper is to describe (i) the use of extensive farm resources by wild...
Article
Semen collection for cryopreservation is a key step for small ruminant conservation programs. While in these species semen is mainly collected via artificial vagina (AV), electroejaculation (EE) provides a viable alternative for untrained males. Herein we investigated the effect of semen collection method on post-thaw sperm quality by comparing two...
Article
The influence of common carcass preparation practices of wild red deer on the physicochemical, microbiological and sensory quality of venison was assessed by varying evisceration time and ageing method. Deer were head shot; half were eviscerated 30 min and the other half 4 h post mortem. In both groups (n = 18), 6 carcasses were skinned immediately...
Article
Full-text available
Although there is ample evidence for the effects of sperm head shape on sperm function, its impact on fertility has not been explored in detail at the intraspecific level in mammals. Here, we assess the relationship between sperm head shape and male fertility in a large-scale study in Manchega sheep (Ovis aries), which have not undergone any select...
Conference Paper
Full-text available
El control de la calidad de la leche en la producción primaria es uno de los problemas técnicos más importantes que intenta abordar del sector ovino lechero. La Asociación Nacional de Ganado Ovino Selecto de raza Manchega (AGRAMA) realiza Programas de mejora y aseguramiento de la calidad de la leche cruda y participa en Proyectos de Investigación d...
Article
Full-text available
Subclinical mastitis is one of the principal health problems of Dairy sheep herds. This problem causes an increase in somatic cell count and breast tissue damage with the consequent milk production loss. From 673.074 somatic cell counts data, belonging to 165 farms to the Manchega Sheep Association of breeders (AGRAMA), have been estimated milk pro...
Article
Full-text available
Field vaccination trials with Mycobacterium bovis BCG, an attenuated mutant of M. bovis, are ongoing in Spain, where the Eurasian wild boar (Sus scrofa) is regarded as the main driver of animal tuberculosis (TB). The oral baiting strategy consists in deploying vaccine baits twice each summer, in order to gain access to a high proportion of wild boa...
Article
Tuberculosis (TB), a chronic disease caused by infection with the Mycobacterium tuberculosis complex, is endemic in wild boar (Sus scrofa) and red deer (Cervus elaphus) in south-central Spain. Understanding the temporal dynamics of this chronic infection requires long time series data collection over large areas. The aim of this paper was to identi...
Article
Full-text available
El sector lácteo español presenta una estructura empresarial de dimensión heterogénea: microestablecimientos, pequeñas y medianas empresas artesanales, y grandes industrias. Las diferencias por volúmenes de negocio, entre unas y otras empresas, son altamente significativas, ya que las diez más grandes son las responsables de la transformación y com...
Conference Paper
In south-central Spain (SCS), the Eurasian wild boar harvest has increased in the last decades in association with more intensive management actions to increase hunting yields and consequent effects on wild boar health status. We investigated the spatialtemporal trend and the risk factors related to the prevalence of Trichinella sp infection in wil...
Article
In south-central Spain, the harvest of Eurasian wild boar (Sus scrofa) has increased significantly during recent decades in association with more intensive management actions to increase hunting yields and with consequent effects on the health status of the wild boar populations. We investigated the spatio-temporal trends and the risk factors relat...
Article
The aim of the present study was to evaluate the effect of selecting a sperm subpopulation by means of a discontinuous density gradient centrifugation (DGC) on the quality of ram thawed semen, and the relationships between sperm parameters assessed in unselected and in selected sperm samples with in vivo fertility after intrauterine artificial inse...
Article
Full-text available
An Aujeszky’s disease (AD) control and eradication campaign started in Spain in 1995, and as a result, AD virus (ADV) seroprevalences have notably diminished in Spanish domestic pig herds, but eradication has not yet been achieved. Since the presence of ADV in Spanish wild boar populations can impede schemes to eradicate ADV in domestic pig, we con...
Article
Computer-assisted sperm morphometry analysis (CASMA) was used in this study to identify sperm morphometric subpopulations in Iberian red deer epididymal sperm samples. Epididymal sperm samples were collected from 37 mature stags and were divided. One portion was diluted in a Tris-citrate-egg yolk medium. A microscope slide was prepared from single...
Article
We have carried out a study on the influence of prolonged cold storage (5 degrees C) of Iberian red deer epididymides on post-thaw sperm motility and DNA integrity. Twenty-nine pairs of testes, with attached epididymides, were collected during November and December. Spermatozoa from one of each of the pairs were immediately recovered, evaluated and...
Article
Full-text available
In the present study, we have related mitochondrial membrane potential (DeltaPsim) and forward scatter (FSC) to apoptotic-related changes in spermatozoa. Thawed red deer spermatozoa were incubated in synthetic oviductal fluid medium (37 degrees C, 5% CO2), with or without antioxidant (100 microm Trolox). At 0, 3, 6 and 9 h, aliquots were assessed f...
Article
With the aim of finding an ideal cryoprotectant (CPA) in a suitable concentration for red deer epididymal spermatozoa cryopreservation, we evaluated the effects of the 3 most commonly used CPAs, glycerol (GLY), ethylene glycol (EG), and propylene glycol (PG), on sperm cryoresistance. The aim of Experiment 1 was to evaluate the influence of 3 differ...
Article
Two experiments were conducted to determine the effects of egg yolk (EY), glycerol, and cooling rate on the cryosurvival of red deer epididymal spermatozoa. The aim of Experiment 1 was to examine the effects of two EY types (clarified EY, CE, prepared by centrifugation, and whole EY, WE), and four EY concentrations (0, 5, 10 and 20%) on cryosurviva...
Article
Full-text available
Computer-automated sperm-head morphometry was used in this study to determine the effects of cryopreservation on red deer sperm-head morphometry. Epididymal sperm samples were collected from 40 mature stags and were divided. One portion was diluted at room temperature in a Tris-citrate egg yolk medium, containing 6% glycerol. A microscope slide was...
Article
Egg yolk is a common component to sperm refrigeration for most of the deer species, the role of which is to protect sperm membranes against cold shock. In addition, there have been many studies of conservation of ejaculated semen from stags, but few have been reported for epididymal spermatozoa. This work was designed to investigate the combined ef...
Article
We studied the seroprevalence of six reproductive pathogens in Spanish hunter-harvested wild boar females. The sample was representative of the hunting harvest in the studied hunting estates. Mean antibody prevalences were: 60.6+/-0.06% for Aujeszky's disease virus (ADV), 56.6+/-0.09% for porcine parvovirus (PPV), 51.8+/-0.06% for porcine circoviru...
Article
Full-text available
With the aim of finding an ideal cryoprotectant in a suitable concentration for red deer epididymal spermatozoa conservation, we evaluated the effects of four most commonly used cryoprotectants (CPAs), Glycerol (G), Ethylene glycol (EG), Propylene glycol (PG), and Dimethyl sulfoxide (DMSO), on the sperm survival. Besides, the effects of two tempera...
Article
A total of 36 873 lactation records of Somatic Cell Counts (SCC) and 32 844 of milk yield, protein and dry matter contents of 28 694 Spanish Manchega ewes from 118 herds were used to estimate genetic parameters under different approaches. In a first step estimations were carried out under multivariate animal models including lactation mean somatic...
Article
Five udder traits, udder depth (UD), udder attachment (UA), teat placement (TP), teat size (TS) and udder shape (US), have been studied in Manchega ewes. The raw data scale, ranging from 1 to 9, was transformed by the Snell method into a new scale from 0 to 100, to achieve more normalised data. Snell transformation was not effective in some cases,...
Article
Full-text available
Dentro del Programa de recuperación de razas y variedades ganaderas en su medio natural desarrollado por la Consejería de Agricultura de Castilla-La Mancha, cabe destacar las actividades realizadas en el Centro Regional de Selección y Reproducción Animal (CERSYRA) de Valdepeñas en Ciudad Real (España), para la conservación de la variedad negra de l...
Article
In the study about the model of genetic evaluation the one including the contemporary group herd-period, the number of lambs at lambing, the combined effect parity-age at lambing and the number of days between lambing and first test-day as fixed effects (GLM6) showed the highest determination coefficient (0.43 for 120D6FCM (individual milk yield st...
Article
El presente estudio se ha desarrollado para investigar si la aplicación de implantes de melatonina podría mejorar las características espermáticas de los moruecos jóvenes de raza Manchega. Para ello, se han empleado dos grupos de corderos de raza Manchega (n=6 animales en cada grupo). Uno de estos lotes recibió un implante de melatonina y el otro s...
Article
Full-text available
El presente estudio se ha desarrollado para investigar si la aplicación de implantes de melatonina podría mejorar las características espermáticas de los moruecos jóvenes de raza Manchega. Para ello, se han empleado dos grupos de corderos de raza Manchega (n=6 animales en cada grupo). Uno de estos lotes recibió un implante de melatonina y el otro s...
Article
Prevalence of subclinical mastitis in 466 Manchega sheep was determined at 3 and 4 mo of lactation. A high proportion of glands and ewes (26.8% and 36.7%, respectively) showed bacterial infection, which was attributable to Staphylococcus in 83.2% of cases. Within this genus, Staphylococcus epidermidis reached 66.8%. Among the indirect diagnostic me...
Article
Full-text available
In order to quantify the incidence of milk proteins on cheesemaking indexes, a study on 3672 Manchega sheep milk samples has been performed. The medium values obtained for those indexes are: curdling time (TC)= 40.13±0.37 min.: medium firmness (DME)=29.49± 1.36 mm.; maximum firmness (DMA)= 60.37±0.69 mm. and rate of firming- (VE)= 42.80±0.37 min. T...
Article
RESUMEN En este trabajo se exponen los resultados generales de fertilidad de los catorce años de empleo de la Inseminación Artificial (IA) en el Esquema de Selección de la Raza Ovina Manchega. Para ello, se han analizado los principales factores de variación. Se hace una comparación de la evolución de la fertilidad, en la segunda mitad del periodo...
Article
Full-text available
-In this paper we present the last works applied on the Manchega sheep breed genetic program. Those works deal with improvements of the evaluation model quality, estimation of variance components and selection response and. extending part-lactation. The inclusion of the effect herd-mobile period, which supposes about a 45% of the phenotypic traits...
Article
El objetivo del presente trabajo ha sido estudiar las tasas de reacción acrosómica (RA) inducidas por el ionóforo de calcio (A23187) en espermatozoides procedentes de moruecos subfértiles o normales, con objeto de analizar las posibilidades de esta prueba como método para discernir entre ambos grupos de sementales. Para ello, se han empleado 3 moru...

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Project (1)
Project
Study of the microbiological, physic-chemical and renneting properties of ewes' milk.