Usmangani Chhalotiya

• M.Pharm, Ph. D
• Professor (Assistant) at Charutar Vidyamandal Institutes

Caesalpinia bonduc is traditionally used in various pharmacological conditions. The various plant parts reflect the presence of various phytoconstituents. The aim of this study was to identify, quantify, and validate selected constituents of cassane diterpenes from C. bonduc leaf extract using thin-layer chromatography‒mass spectrometry (TLC‒MS). A...

A plant with potential, Amorphophallus paeoniifolius, which has many medicinal values, belongs to the Araceae family. An accurate and precise method for the quantification of two biomarkers in the tuber extract of A. paeoniifolius has been developed and validated using instrumental thin-layer chromatography (TLC). The planar chromatographic method...

A significant disease impacting quality of life is asthma, a pulmonary condition. Amorphophallus paeoniifolius (Donnst) Nicolson, traditionally used for allergic asthma belonging to Araceae family, shows therapeutic potential against inflammation. Till today scientific validation of this plant specifically molecular mechanisms against asthma remain...

Combination of Metoprolol Succinate and Dapagliflozin is recommended in the treatment of Hypertension. Accurate and precise thin layer chromatographic method has been developed for the determination of Metoprolol Succinate and Dapagliflozin in combination. Pre-coated silica gel G60—F254 aluminium sheet (10 × 10 cm),0.2 mm layer thickness used as st...

For the treatment of Type 2 Diabetes Mellitus, Dapagliflozin selectively inhibits the sodium-glucose co-transporter-2, while Linagliptin competitively and reversibly inhibits dipeptidyl peptidase-4 in a fixed dose combination (1:1). Dapagliflozin and Linagliptin estimation in bulk and tablet formulation was accomplished by the development and valid...

Newer drugs and drugs combinations for treatment of different diseases are approved by regulatory authorities. Such new combination of existing drugs like Lidocaine HCl and Diltiazem HCl has been approved for anal fissure treatment. The present study represents validated method for estimation of Lidocaine HCl and Diltiazem HCl in a combined gel dos...

For the treatment of Type 2 Diabetes Mellitus, Dapagliflozin selectively inhibits the sodium-glucose co-transporter-2, while Linagliptin competitively and reversibly inhibits dipeptidyl peptidase-4 in a fixed dose combination (1:1). Dapagliflozin and Linagliptin estimation in bulk and tablet formulation was accomplished by the development and valid...

Anemia with chronic kidney disease treating newer hope is recently approved Desidustat. By following International Conference on Harmonization Quality guideline Q2 (R1), an economic high‐performance thin‐layer chromatography method has been developed for the estimation of Desidustat. Pre‐coated silica gel 60 F 254 plates as stationary phase along w...

A combination of Azilsartan Medoxomil and Cilnidipine is prescribed in the treatment of Hypertension. The present work represents an accurate and precise high-performance thin layer chromatographic method for the estimation of Azilsartan Medoxomil (AZL) and Cilnidipine (CLN) in combined tablet dosage form. Pre-coated silica gel- G60 F254 aluminum s...

The literature review reveals that no thin‐layer chromatographic method has been developed for the simultaneous estimation of Bisoprolol fumarate and Ivabradine HCl in the combined dosage form. A sensitive, precise, accurate, and robust thin‐layer chromatographic method was developed and validated for the simultaneous quantification of a newer comb...

A combination of Azilsartan Medoxomil and Cilnidipine is prescribed in the treatment of Hypertension. An accurate and precise high‐performance liquid chromatographic method has been developed for the estimation of Azilsartan Medoxomil and Cilnidipine in a combined tablet dosage form. Symmetry C18 (250 × 4.6 mm, 5 μm) column was used as the stationa...

A second-order derivative spectrophotometric method is developed and validated for the simultaneous estimation of Azilsartan Medoxomil and Cilnidipine in Tablet dosage form. The determination of Azilsartan Medoxomil in the presence of Cilnidipine was achieved by measuring the second-order derivative signals at 231 nm, while the second-order derivat...

For the simultaneous quantification of Ivabradine HCl and Bisoprolol fumarate in bulk and synthetic mixtures, a sensitive, precise, robust, and accurate reverse‐phase–high‐performance liquid chromatographic technique was developed and validated according to the International Council for Harmonization of Technical Requirements for Pharmaceuticals fo...

A simple UV spectrophotometric analytical method has been developed for simultaneous estimation of Ivabradine HCl and Bisoprolol used in the treatment of angina pectoris. Modied simultaneous equation method was developed with double beam UV-Visible spectrophotometer using water as a solvent. The method was found to be linear in the range of 4-16 µ...

Background A sensitive, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method has been developed for the analysis of Remogliflozin etabonate in tablet formulation. HPTLC plates precoated with silica gel 60 F 254 were used as the stationary phase; methanol: ethyl acetate: toluene: NH 3 (2:4:4:0.1, v/v/v) was us...

Background Extensive literature review revealed that no RP–LC method has been developed for simultaneous estimation of EMPA, LINA and MET in combined dosage form. This is a newer combination approved by USFDA on 4th June 2019 and it is launch in the United State Market on 27th January 2020. Result A simple, sensitive, specific, precise and accurat...

A precise, accurate and sensitive high-performance thin-layer chromatographic method has been developed and validated for the simultaneous determination of montelukast and bilastine in a tablet dosage form which is used in the treatment of allergic rhinitis. The proposed method used aluminum plates pre-coated with silica gel 60F254 as the stationar...

DOI: 10.1002/sscp.202100018 The cover picture shows a densitogram of base hydrolyzed sample of Ibrutinib that has a well separated drug and degradant peak.

A sensitive, selective, accurate, precise and robust thin layer chromatographic method has been developed and validated for the estimation of β-Adrenergic Receptor agonist drug Mirabegron in bulk and pharmaceutical formulation. Aluminium supported plates pre-coated with silica gel 60F-254 was used as stationary phase while methanol: acetonitrile: t...

A precise, accurate, and sensitive thin‐layer chromatographic method was developed for the estimation of anticancer drug Ibrutinib in synthetic mixture. The developed method uses precoated silica gel 60 F254 aluminum plates as stationary phase and Methanol: Ethyl acetate: Ammonia (2: 8: 0.2, v/v/v) as mobile phase. The densitometric analysis was ca...

An accurate, sensitive, robust and precise high performance thin layer liquid chromatography method was developed based on ICH Q2 (R1) guidelines for estimation of novel combination of Amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium in bulk and its synthetic mixture. Pre-coated silica gel aluminum plate 60 F254 was selected as th...

A sensitive, accurate, precise, and robust high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of empagliflozin, linagliptin, and metformin HCl in bulk and synthetic mixture. The method applied HPTLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase along with n-b...

The proposed sensitive, precise, and accurate reverse phase-liquid chromatography (RP-LC) method is used for simultaneous estimation Amlodipine besylate, Rosuvastatin calcium and Fimasartan potassium trihydrate in bulk and synthetic mixture which is under phase 3 trial. An accurate and cost effective isocratic approach is employed by using Phenomen...

DOI: 10.1002/sscp.202000010 The cover picture shows the HPTLC method developed for the simultaneous identification and quantification of Perindopril erbumine, Indapamide and Amlodipine besylate in bulk and pharmaceutical dosage form. According to ICH guidelines the method was validated and found to be sensitive, accurate and precise. Statistical an...

A sensitive, precise, robust and accurate high performance thin layer chromatographic method was developed and validated base on ICH Q2 (R1) guideline for the simultaneous estimation of Perindopril erbumine, Indapamide and Amlodipine besylate in bulk and combined Triplixam marketed tablet dosage formulation. Pre‐coated silica gel aluminum plate 60...

A precise and accurate method for the analysis of antihypertensive drug combination nifedipine and valsartan was developed using the HPTLC method. The stationary phase used was a pre-coated silica gel G60 – F254 aluminium sheet and the mobile phase was acetonitrile: methanol: n-butanol: acetic acid (6:2:2:0.1, v/v/v/v). The detection of spots was c...

A sensitive, selective and precise high performance thin layer chromatographic method has been developed and validated for the quantification of Brexpiprazole in bulk drug and in pharmaceutical dosage form. The method employed HPTLC aluminum plates (pre-coated with silica gel 60 F254) as stationary phase while n-butanol was used as mobile phase. Th...

A sensitive and precise high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of clozapine and aripiprazole in combination. The method employed HPTLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase, while the solvent system was toluene‒methanol‒ethyl acetate‒ammonia...

A precise and accurate HPTLC method for simultaneous estimation of pioglitazone HCl (PIO) and alogliptin benzoate (ALO) in synthetic mixture has been developed. The stationary phase selected was Pre-coated TLC plates (silica gel G60-F254 aluminium sheet) and the mobile phase selected was n-butanol : water : acetic acid (6 : 3 :1 V/V/V). The separat...

A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of Ticagrelor in pharmaceutical dosage form. The method employed Thin-layer chromatography (TLC) aluminum plates precoated with silica gel 60 F254 as the stationary phase, while the solvent system was found to be toluene: Ethyl...

A sensitive, selective and precise liquid chromatographic method has been developed and validated for simultaneous determination of moxonidine and amlodipine besylate both as bulk drug and in formulation. Phenomenex Luna C18 column (250 × 4.6 mm id, 5um particle size) chromatographic column equilibrated with mobile phase methanol: acetonitrile: 0.0...

Quantitative determination of Clofarabine in their Pharmaceutical Dosage form was carried out by Liquid chromatographic method. The aim of the present work was to develop a RP-HPLC method for Clofarabine (CFB). The column used was Luna C-18 column (250 mm × 4.6 mm id, 5 m particle size) using Water: Methanol (60:40 v/v) as a mobile phase at a flow...

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent sy...

A sensitive, selective and precise high-performance thin-layer chromatographic method was developed and validated for the simultaneous determination of alprazolam and mebeverine, both as bulk drugs and in formulations. The method employed HPTLC aluminium plates that had been pre-coated with silica gel 60F-254 as the stationary phase, while the solv...

A simple, accurate and precise first order derivative spectrophotometric method was developed for simultaneous determination of Metolazone (MET) and Ramipril (RAM) in their combined pharmaceutical dosage form. MET and RAM were quantified using first derivative responses at 285nm and 212nm respectively. Methanol was taken as a common solvent. The ca...

Liquid chromatographic method was developed for simultaneous quantitative determination of dicyclomine hydrochloride, mefenamic acid and paracetamol in their combined dosage form. The separation was achieved using a C18 column (250×4.6 mm id, 5 μm) using acetonitrile:20 mM potassium dihydrogen phosphate 70:30 (v/v) adjusted to pH 4 using orthophosp...

A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of Irbesartan and Amlodipine besylate in combination. The method employed Thin-layer chromatography (TLC) aluminum plates precoated with silica gel 60 F254 as the stationary phase, while the solvent system was chloroform: toluen...

A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of torsemide and amiloride hydrochloride in the pharmaceutical dosage form. Thin-layer chromatographic (TLC) aluminum plates precoated with silica gel 60 F 254 were used as the stationary phase, while chloroform-methanol- ammoni...

A new rapid, selective and sensitive liquid chromatography–tandem mass spectrometric method was developed and validated for the determination of ciprofibrate, an antihyperlipidemic agent, in K2EDTA human plasma. Furosemide was used as internal standard (IS). The ciprofibrate and IS were extracted using Oasis HLB 1 cc 30 mg solid-phase extraction ca...

A rapid, specific and sensitive stability indicating reverse phase high performance liquid chromatographic method has been developed and validated for analysis of clioquinol in both bulk and pharmaceutical dosage form. A Sunfire C18, 4.5μm column with mobile phase containing acetonitrile-water pH 3 adjusted with 1% o- phosphoric acid (90:10, v/v) w...

Liquid chromatographic method was developed for the quantitative determination of diethyl caibamazine citrate and chlorpheniramine maleate in combined dosage form, A Sunfire C18 5 μm (250 mm × 4.6 mm i.d.) particle size column with mobile phase containing water: methanol: 10% triethyl amine (10:90:0.1, v/v; pH 5.5) was used. The flow rate was 1.0 m...

A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of paracetamol, dicyclomine hydrochloride, and mefenamic acid in combined dosage form. The method employed high-performance thin-layer chromatography (HPTLC) aluminum plates precoated with silica gel 60 F-254 as the stationary p...

A simple, sensitive, and precise high performance thin layer chromatographic method (HPTLC) has been developed for the estimation of ibuprofen and famotidine in combined dosage form. The method employed HPTLC aluminum plates precoated with silica gel 60F254 as the stationary phase while the solvent system was methanol: ethyl acetate: hexane: ammoni...

Simple, accurate, precise, reproducible, requiring no prior separation, and economical procedures for simultaneous estimation of benazepril hydrochloride (BEN) and Hydrochlorothiazide (HCT) in tablet dosage form have been developed. Simultaneous equation method employs for estimation of both drugs in methanol at 240 nm and 270 nm as two analytical...

A simple, accurate, and precise spectrophotometric method was developed for simultaneous estimation of pantoprazole sodium and levosulpiride in capsule dosage form. Simultaneous equation was developed at 290 and 232 nm. The method was found to be linear in the range of 4–12 μg/mL for pantoprazole sodium and 8–20 μg/mL for levosulpiride while accura...

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of levocetirizine dihydrochloride (LCT) and phenylephrine hydrochloride (PHE) by employing first-order derivative spectrophotometric method. The first-order derivative absorption at 240 nm (zero crossing point of PHE) was used for qu...

A rapid, specific, and sensitive reverse phase high performance liquid chromatographic method has been developed and validated for analysis of tolperisone in both bulk and pharmaceutical dosage form. The HPLC method was performed with a reversed phase C18 SunFire column (250 mm × 4.6 mm i.d., 5 mm particle size), detection at 261 nm and a mixture o...

A rapid, specific and sensitive stability indicating reverse phase high performance liquid chromatographic method has been developed and validated for analysis of levosulpiride hydrochloride in both bulk and pharmaceutical dosage form. An isocratic stability indicating reversed-phase liquid chromatographic determination was developed for the quanti...

A total of 500 questionnaire were distributed in the college premises with respondent rate of 450(90%).majority of respondents, 73% were aged between 18 269(59.5%) were female and 181(40.5%) were male. The survey was conducted in the colleg Ayurvedic, Biotechnology, Engineering and pharmacy. The survey comprised of 290 students (64.4%) including bo...

A simple and accurate method for the analysis of ibuprofen (IBU) and famotidine (FAM) in their combined dosage form was developed using second order derivative spectrophotometery. IBU and FAM were quantified using second derivative responses at 272.8 nm and 290 nm in the spectra of their solutions in methanol. The calibration curves were linear in...

A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method is described for simultaneous analysis of metoprolol succinate and olmesartan medoxomil in pharmaceutical formulations. The separations were achieved on prepared TLC plates precoated with silica gel G 60 F254 and the plates were developed with methanol-e...

A simple, specific and stability-indicating reversed phase high performance liquid chromatographic method was developed for the quantitative determination of asenapine in tablet dosage form. A SunFire C(18), 5 μm column having 250×4.6 mm i.d. in isocratic mode, with mobile phase containing 0.02 M potassium dihydrogen phosphate: acetonitrile (95:05,...

An isocratic, reversed phase-liquid-chromatographic assay method was developed for the quantitative determination of ibuprofen and famotidine in combined-dosage form. A Brownlee C18, 5 μm column with mobile phase containing water : methanol : acetonitrile (30 : 60 : 10, v/v/v) was used. The flow rate was 1.0 mL/min, and effluents were monitored at...

Liquid chromatographic method for the quantitative determination of iloperidone in the pharmaceutical dosage form was developed. A reversed phase column with mobile phase containing 0.02 M potassium dihydrogen phosphate: methanol: acetonitrile (55 : 25 : 20, v/v/v, pH 4 adjusted with phosphoric acid) was used. The flow rate was 1.0 mL min−1, and ef...

A sensitive, selective and precise high performance thin layer chromatographic method has been developed and validated for the simultaneous determination of melitracen hydrochloride and flupentixol dihydrochloride both as a bulk drug and in formulation. The method employed HPTLC aluminium plates pre-coated with silica gel 60F-254 as the stationary...

An isocratic stability indicating reversed-phase liquid chromatographic determi-nation was developed for the quantitative determination of lacosamide in the pharmaceutical dosage form. A Hypersil C-18, 4.5 μm column with mobile phase containing acetonitrile-water (20:80, v/v) was used. The flow rate was 1.0 mL min -1 and effluents were monitored at...

A simple, sensitive, precise and stability indicating liquid chromatographic method has been developed for the estimation of desvenlafaxine succinate in pharmaceutical dosage form. A Hypersil C-18, 5 mu m-column with a mobile phase containing 0.05 M potassium dihydrogen phosphate:methanol (60:40 v/v), pH 7, was used. The flow rate was 1.0 mL/min an...

A simple, specific and stability indicating reversed phase high performance liquid chromatographic method was developed for the simultaneous determination of paracetamol and lornoxicam in tablet dosage form. A Brownlee C-18, 5 μm column having 250×4.6 mm i.d. in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen phosphate:meth...

A simple, specific and stability-indicating reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of melitracen hydrochloride and flupentixol dihydrochloride in tablet dosage form. A Brownlee C-18, 5 μm column having 250 × 4.6 mm i.d. in isocratic mode, with mobile phase containing 0.025 M po...

A stability indicating HPLC method for the estimation of donepezil hydrochloride in tablets was developed and validated. Donepezil hydrochloride is a reversible inhibitor of acetylcholinesterase, indicated for the treatment of mild to moderate dementia of the Alzheimer's type. The HPLC method was performed with a reversed phase C 18 column (250 mm...

A simple, specific, accurate, and precise ultra performance liquid chromatographic method was developed and validated for the estimation of venlafaxine hydrochloride in tablet dosage forms. A acquity TM BEH column having C18, 100×2.1 mm i.d. in isocratic mode, with mobile phase containing dipotassium hydrogen phosphate: Acetonitrile (30:70 v/v; pH...

The objective of the present work was to develop a stability-indicating RP-HPLC method for duloxetine hydrochloride (DUL) in the presence of its degradation products generated from forced decomposition studies. The drug substance was found to be susceptible to stress conditions of acid hydrolysis. The drug was found to be stable to dry heat, photod...