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Urszula Slawinska

Urszula Slawinska
Nencki Institute of Experimental Biology, PAS

PhD

About

88
Publications
7,114
Reads
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1,480
Citations
Citations since 2016
30 Research Items
672 Citations
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Additional affiliations
January 1998 - present
Nencki Institute of Experimental Biology, PAS
Position
  • Principal Investigator
Education
October 1976 - February 2002
University of Warsaw
Field of study
  • Physics

Publications

Publications (88)
Article
Full-text available
Traumatic injury of the spinal cord (SCI) is a devastating neurological condition often leading to severe dysfunctions, therefore an improvement in clinical treatment for SCI patients is urgently needed. The potential benefits of transplantation of various cell types into the injured spinal cord have been intensively investigated in preclinical SCI...
Article
Full-text available
Coordination of four-limb movements during quadrupedal locomotion is controlled by supraspinal monoaminergic descending pathways, among which serotoninergic ones play a crucial role. Here we investigated the locomotor pattern during recovery from blockade of 5-HT7 or 5-HT2A receptors after intrathecal application of SB269970 or cyproheptadine in ad...
Article
Full-text available
Introduction CacyBP/SIP is a multifunctional protein present in various mammalian tissues, among them in brain. Recently, it has been shown that CacyBP/SIP exhibits phosphatase activity towards ERK1/2 and p38 kinases. Objectives The aim of our study was to analyze the localization and level of CacyBP/SIP and its substrates, phosphorylated ERK1/2 (...
Article
Full-text available
Intraspinal grafting of serotonergic (5-HT) neurons was shown to restore plantar stepping in paraplegic rats. Here we asked whether neurons of other phenotypes contribute to the recovery. The experiments were performed on adult rats after spinal cord total transection. Grafts were injected into the sub-lesional spinal cord. Two months later, locomo...
Article
Full-text available
Applying serotonergic (5-HT) agonists or grafting of fetal serotonergic cells into the spinal cord improves locomotion after spinal cord injury. Little is known about the role of 5-HT receptors in the control of voluntary locomotion, so we administered inverse agonists of 5-HT2 (Cyproheptadine; Cypr), 5-HT2A neutral antagonist (Volinanserin; Volin)...
Article
Full-text available
Serotonin (5-hydroxytryptamine; 5-HT) plays an important role in control of locomotion, partly through direct effects on motoneurons. Spinal cord complete transection (SCI) results in changes in 5-HT receptors on motoneurons that influence functional recovery. Activation of 5-HT 2A and 5-HT 7 receptors improves locomotor hindlimb movements in parap...
Article
Findings from mice that had their Smn gene deleted and some copies of the human SMN2 gene introduced to produce SMN protein are summarized. Symptoms due to this manipulation can be corrected only by restoring the SMN protein expression in neurones and not in muscle. The changes in muscle and neuromuscular junction (NMJ) in these mutant mice are pro...
Article
Full-text available
Oscillatory rhythms in local field potentials (LFPs) are thought to coherently bind cooperating neuronal ensembles to produce behaviors, including locomotion. LFPs recorded from sites that trigger locomotion have been used as a basis for identification of appropriate targets for deep brain stimulation (DBS) to enhance locomotor recovery in patients...
Data
The relationship between the burst duration of EDL muscle EMG activity and the duration of cycle. The table contains slopes and intercepts of regression with the values of p for their significance as well as correlation coefficients r in individual intact, saline and Riluzole treated animals. Abbreviations: L/Co-left/control, R/SNC-right/muscle wit...
Data
The duration and duty factor of burst of Sol muscle EMG activity. The table contains mean (±SD) cycle duration, burst duration and duty factor in individual rats and in groups of intact, saline and Riluzole treated animals. The values of SEM ranged from 0.71 to 1.67%. Abbreviations: L/Co-left/control, R/SNC-right/muscle with SNC. Abbreviations for...
Data
The duration of IS-E and IE-S intervals and the relationship with the burst duration of Sol muscle EMG activity. The table contains mean (±SD) of interval durations, predicted durations, slopes and intercepts of regressions as well as correlation coefficients r with the values of p obtained in individual rats and in group of intact rats for the rig...
Data
The relationship between the duty factor established for right and left hindlimbs (with SNC and control). The table contains correlation coefficients r with corresponding values of p for significance, for the relationship between the duty factor of burst of EMG activity of right muscle/muscle with SNC and the duty factor of burst of EMG activity of...
Data
The duration of IS-E and IE-S intervals and the relationship with the burst duration of Sol muscle EMG activity. The table contains mean (±SD) of interval durations, predicted durations, slopes and intercepts of regressions as well as correlation coefficients r with the values of p obtained in individual rats and in group of intact rats for the lef...
Data
The phase shift and strength of intralimb and interlimb coordination. The table contains mean (± circular SD) of phase shifts of intralimb (L/Co Sol—L/Co EDL and R/SNC Sol—R/SNC EDL) and interlimb (R/SNC Sol—L/Co Sol and R/SNC EDL—L/Co EDL) coordination and r-values obtained with Polar Plot analysis in individual rats and in groups of intact, salin...
Data
The duration of cycle. The table contains mean (±SD) of cycle durations established based on the left and right Sol and EDL muscles, on control muscles and muscles with SNC in individual rats and in groups of intact, saline and Riluzole treated animals. The values of SEM ranged from 1.78 to 3.20%. Abbreviations: L/Co-left/control, R/SNC-right/muscl...
Data
The relationship between the burst duration of Sol muscle EMG activity and the duration of cycle. The table contains slopes and intercepts of regression with the values of p for significance of intercepts and correlation coefficients r in individual intact, saline and Riluzole treated animals. The values of p or significance of slopes and correlati...
Data
The duration and duty factor of burst of EDL muscle EMG activity. The table contains mean (±SD) cycle duration, burst duration and duty factor in individual rats and in groups of intact, saline and Riluzole treated animals. The values of SEM ranged from 0.57 to 3.96%. Abbreviations: L/Co-left/control, R/SNC-right/muscle with SNC. Abbreviations for...
Article
Full-text available
The effects of sciatic nerve crush (SNC) and treatment with Riluzole on muscle activity during unrestrained locomotion were identified in an animal model by analysis of the EMG activity recorded from soleus (Sol) and extensor digitorum longus (EDL) muscles of both hindlimbs; in intact rats (IN) and in groups of rats treated for 14 days with saline...
Data
The duration of cycle. The table contains mean (±SD) of cycle durations established based on the left and right Sol and EDL muscles, on control muscles and muscles with SNC in individual rats and in groups of intact, saline and Riluzole treated animals. The values of SEM ranged from 1.78 to 3.20%. Abbreviations: L/Co-left/control, R/SNC-right/muscl...
Data
The duration and duty factor of burst of Sol muscle EMG activity. The table contains mean (±SD) cycle duration, burst duration and duty factor in individual rats and in groups of intact, saline and Riluzole treated animals. The values of SEM ranged from 0.71 to 1.67%. Abbreviations: L/Co-left/control, R/SNC-right/muscle with SNC. Abbreviations for...
Data
The relationship between the burst duration of Sol muscle EMG activity and the duration of cycle. The table contains slopes and intercepts of regression with the values of p for significance of intercepts and correlation coefficients r in individual intact, saline and Riluzole treated animals. The values of p for significance of slopes and correlat...
Data
The relationship between the burst duration of EDL muscle EMG activity and the duration of cycle. The table contains slopes and intercepts of regression with the values of p for their significance as well as correlation coefficients r in individual intact, saline and Riluzole treated animals. Abbreviations: L/Co-left/control, R/SNC-right/muscle wit...
Data
The phase shift and strength of intralimb and interlimb coordination. The table contains mean (± circular SD) of phase shifts of intralimb (L/Co Sol—L/Co EDL and R/SNC Sol—R/SNC EDL) and interlimb (R/SNC Sol—L/Co Sol and R/SNC EDL—L/Co EDL) coordination and r-values obtained with Polar Plot analysis in individual rats and in groups of intact, salin...
Data
The duration of IS-E and IE-S intervals and the relationship with the burst duration of Sol muscle EMG activity. The table contains mean (±SD) of interval durations, predicted durations, slopes and intercepts of regressions as well as correlation coefficients r with the values of p obtained in individual rats and in group of intact rats for the lef...
Data
The duration of IS-E and IE-S intervals and the relationship with the burst duration of Sol muscle EMG activity. The table contains mean (±SD) of interval durations, predicted durations, slopes and intercepts of regressions as well as correlation coefficients r with the values of p obtained in individual rats and in group of intact rats for the rig...
Data
The duration and duty factor of burst of EDL muscle EMG activity. The table contains mean (±SD) cycle duration, burst duration and duty factor in individual rats and in groups of intact, saline and Riluzole treated animals. The values of SEM ranged from 0.57 to 3.96%. Abbreviations: L/Co-left/control, R/SNC-right/muscle with SNC. Abbreviations for...
Data
The relationship between the duty factor established for right and left hindlimbs (with SNC and control). The table contains correlation coefficients r with corresponding values of p for significance, for the relationship between the duty factor of burst of EMG activity of right muscle/muscle with SNC and the duty factor of burst of EMG activity of...
Article
Key points: Experiments on neonatal rodent spinal cord showed that serotonin (5-HT), acting via 5-HT7 receptors, is required for initiation of locomotion and for controlling the action of interneurons responsible for inter- and intralimb coordination, but the importance of the 5-HT system in adult locomotion is not clear. Blockade of spinal 5-HT7...
Article
Full-text available
Lateral thoracic hemisection of the rodent spinal cord is a popular model of spinal cord injury, in which the effects of various treatments, designed to encourage locomotor recovery, are tested. Nevertheless, there are still inconsistencies in the literature concerning the details of spontaneous locomotor recovery after such lesions, and there is a...
Article
Locomotion-a fundamental patterned movement expressed as a result of multiple behavior and emotional states-arises due to complex forebrain, diencephalic, and brainstem networks converging on the relative automatism of the spinal central pattern generator. Over 100. years ago, Sherrington and Graham-Brown provided some of the most important breakth...
Article
Full-text available
Previous experiments implicate cholinergic brainstem and spinal systems in the control of locomotion. Our results demonstrate that the endogenous cholinergic propriospinal system, acting via M2 and M3 muscarinic receptors, is capable of consistently producing well-coordinated locomotor activity in the in vitro neonatal preparation, placing it in a...
Article
Full-text available
The gelatinases MMP-9 and MMP-2 have been implicated in skeletal muscle adaptation to training; however, their specific role(s) in the different muscle types are only beginning to be unraveled. Recently, we found that treadmill running increased the activity and/or expression of these enzymes in myonuclei and in activated satellite cells of the sol...
Article
Full-text available
There is considerable evidence from research in neonatal and adult rat and mouse preparations to warrant the conclusion that activation of 5-HT2 and 5-HT1A/7 receptors leads to activation of the spinal cord circuitry for locomotion. These receptors are involved in control of locomotor movements, but it is not clear how they are implicated in the re...
Article
Full-text available
In this review we will discuss different ways for re-establishing serotonergic activity that can enhance recovery of coordinated plantar stepping after spinal cord injury in adult rats. It is well known that serotoninergic neurons located in the medulla are able to initiate locomotor activity. This effect is exerted by actions on motoneurons and on...
Article
In rodent models of spinal cord injury, there is increasing evidence that activation of the locomotor central pattern generator (CPG) below the site of injury with 5-hydroxytryptamine (5-HT) agonists improves locomotor recovery and restores coordination. A promising means of replacing 5-HT control of locomotion is to graft brainstem 5-HT neurons in...
Article
Full-text available
Myosin VI (MVI) is a unique unconventional motor moving backwards on actin filaments. In non-muscle cells, it is involved in cell migration, endocytosis and intracellular trafficking, actin cytoskeleton dynamics, and possibly in gene transcription. An important role for MVI in striated muscle functioning was suggested in a report showing that a poi...
Article
Full-text available
Van den Brand et al. (Reports, 1 June 2012, p. 1182) claim to have restored voluntary control of locomotion after paralyzing spinal cord injury. They have not considered recent findings that their upright posture paradigm contributes to locomotor capability after such injuries. We propose that postural adjustments that activate the locomotor centra...
Article
Full-text available
Matrix metalloproteinases (MMPs) are key regulators of extracellular matrix remodeling, but have also important intracellular targets. The purpose of this study was to examine the activity and subcellular localization of the gelatinases MMP-2 and MMP-9 in skeletal muscle of control and physically trained rats. In control hind limb muscle, the activ...
Data
Suppl. Fig. The representation of a semi-automatic algorithm used for quantitative analysis of the myonuclear gelatinolytic activity in sections subjected to in situ zymography. The analysis is performed in the images (A as an example) of the specimens triple-labeled for (i) β-dystroglycan, a marker of sarcolemma (Alexa 555, red in A), (ii) gelatin...
Article
Recent studies on the restoration of locomotion after spinal cord injury have employed robotic means of positioning rats above a treadmill such that the animals are held in an upright posture and engage in bipedal locomotor activity. However, the impact of the upright posture alone, which alters hindlimb loading, an important variable in locomotor...
Article
Nerve injury in neonatal rats leads to considerable motoneuron death. We investigated whether treatment with riluzole (a presynaptic inhibitor of glutamate release) is able to enhance survival of motor units (MUs) in the slow soleus (Sol) and fast extensor digitorum longus (EDL) muscles after sciatic nerve crush in newborn rats. Examination of 3- t...
Article
Three rhythmic movements, breathing, walking, and chewing, are considered from the perspective of the emerging factors that control their coordination. This takes us beyond the concept of a core excitatory kernel and into the common principles that govern the interaction between components of the neural networks that must be orchestrated properly t...
Chapter
At the Summer School for Biological Treatment of Chronic Spinal Cord Injury, held in Vienna in October 2008, some approaches tested on patients, based on experimental work on animals to encourage regeneration of axons in the central nervous system, were discussed. These included problems of overcoming the effect of the unfavorable environment of th...
Chapter
This chapter presents animal models of spinal cord injury (SCI) and discusses how an understanding of the mechanisms responsible for producing the locomotor pattern, rhythmic limb movements associated with locomotion, is useful for the development of interventions to repair the damaged spinal cord. It reviews the work performed in cat and rat model...
Article
Full-text available
The relationships between the stimulation frequency and the force developed by motor units (MUs) of the medial gastrocnemius muscle were compared between intact rats and animals after total transection or hemisection of the spinal cord at the low thoracic level. The experiments on functionally isolated MUs were carried out 14, 30, 90, and 180 days...
Article
The contractile properties of motor units (MUs) were electrophysiologically investigated in the medial gastrocnemius (MG) muscle in 17 Wistar three-month-old female rats: 14, 30, 90 and 180 days after the total transection of the thoracic spinal cord and compared to those in intact (control) rats. A sag phenomenon, regularly observed in unfused tet...
Article
The contractile properties of motor units (MUs) were investigated in the medial gastrocnemius (MG) muscle in rats after the spinal cord hemisection at a low thoracic level. Hemisected animals were divided into 4 groups: 14, 30, 90 and 180 days after injury. Intact rats formed a control group. The mass of the MG muscle did not change significantly a...
Article
CD44 is a multifunctional cell surface glycoprotein which regulates cell-cell and cell-matrix interactions in a variety of tissues. In particular, the protein was found to be expressed in glial cells of developing, but not adult, peripheral nerves, where it takes part in signaling mediated by ErbB class of receptors for neuregulins. Here, we demons...
Article
Full-text available
The effect of stimuli predicting danger (DS) and safety (SS) in Pavlovian aversive conditioning on hippocampal local field potentials (LFP) was studied in 25 partially restrained adult male rats (Long-Evans). DS lasting 5 s preceded tail-shock, while SS overlapping DS during DS last 3 s predicted omission of shock. The power spectra of LFPs during...
Article
The main aim of the study was to investigate whether neurones in the ipsilateral red nucleus (NR) affect hindlimb motoneurones. Intracellular records from motoneurones revealed that both EPSPs and IPSPs were evoked in them via ipsilaterally located premotor interneurones by stimulation of the ipsilateral NR in deeply anaesthetized cats in which onl...
Article
Full-text available
In the medial gastrocnemius muscle of intact rats, division of motor units (MUs) into slow (S) or fast (F) types is typically based on presence of a sag phenomenon in 40 Hz unfused tetanic contraction. MUs with sag are classified as F, while those without sag as S. However, in rats one month after spinal cord injury this phenomenon almost completel...
Article
Full-text available
Mechanism of denervation atrophy remains poorly understood. In particular, the question about irreversibility of the late atrophy is still open. Therefore, in the present study, we investigated whether and how a passive movement can affect a progress of atrophy in rat soleus muscle. To address this issue, a locomotor training on a treadmill was app...
Article
Full-text available
In order to study mechanisms of muscle denervation atrophy, a locomotor training on a treadmill was applied to denervated rat soleus muscle. Ultrastructure of the treated muscles were investigated two months after denervation. It has been found that pathological changes caused by denervation were less pronounced in trained muscle comparing to untra...
Article
Changes in locomotor movements induced by central and peripheral nerve injury or obtained as a result of pharmacological treatment are increasingly being investigated in rats. Several methods have been used to assess changes in the main locomotor indices, most of which are based on video recordings, usually with low time resolution, or on X-ray cin...
Chapter
In the present chapter we described the use of neural tissue transplantation for enhancing the recovery of function that has been lost after injury of the spinal cord. Attempts to replace the descending monoaminergic inputs by embryonic grafts from the brain stem raphe nuclei to activate the remaining intact circuitry of the spinal cord below the l...
Article
We have investigated the effect of precociously increasing locomotor activity during early postnatal development by daily treatment with the monoaminergic precursor L-DOPA on the survival of motoneurones supplying the slow soleus (SOL) muscle and the fast, tibialis anterior (TA) and extensor digitorum longus (EDL) muscles as well as the contractile...
Article
Full-text available
More than a hundred years of extensive studies have led to the development of clinically valid animal models of spinal cord injury (SCI) used to investigate neurophysiological mechanisms, pathology and potential therapies. The cat and rat models of SCI were found particularly useful due to several behavioral responses that correspond to clinical sy...
Article
The effects of upper lumbar level intrathecal injection of yohimbine, an alpha2-noradrenergic antagonist, on overground locomotion in intact rats was studied. This treatment caused dose-dependent impairment of hindlimb locomotor movement, which varied from transient hindlimb paralysis at a dose of 200 microg/20 microl to transient trunk instability...
Article
The effects of complete transection of the spinal cord at the level of Th9/10 on contractile properties of the motor units (MUs) in the rat medial gastrocnemius (MG) muscle were investigated. Our results indicate that 1 month after injury the contraction time (time-to-peak) and half-relaxation time were prolonged and the maximal tetanic force in mo...
Article
Full-text available
Recently, we demonstrated improvements in hind limb locomotor-like movements following grafting of embryonic raphe nuclei cells into the spinal cord below the level of total transection in adult rats. The purpose of the present study was to clarify whether this improvement was due to newly established serotonergic innervation between the graft and...
Article
Pathways through which reticulospinal neurons can influence contralateral limb movements were investigated by recording from motoneurons innervating hindlimb muscles. Reticulospinal tract fibers were stimulated within the brainstem or in the lateral funiculus of the thoracic spinal cord contralateral to the motoneurons. Effects evoked by ipsilatera...
Article
Full-text available
Human patients suffering from motor paralysis of the leg can learn, to some extent, how to use the transposed antagonistic muscle in place of the damaged or ineffective muscle. Experiments on animals showed opposite results, although in a few experiments the functional reorganization of the activity of the transposed muscle was not excluded. In our...