Urszula Kozlowska

Urszula Kozlowska
Polish Academy of Sciences | PAN · Institute of Bioorganic Chemistry of the Polish Academy of Sciences

Doctor of Philosophy in Biology

About

14
Publications
3,069
Reads
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330
Citations
Citations since 2016
13 Research Items
329 Citations
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Introduction
PhD in stem cell biology, interested in regenerative and translational medicine. Currently working on the influence of magic mushrooms alkaloids, LSD and DMT on neural tissue homeostasis and their prospect application for polyQ diseases treatment. Also gene therapies. Secretly dreaming about conducting research on consciousness :)
Additional affiliations
March 2019 - present
Instytut Immunologii i Terapii Doświadczalnej im. Ludwika Hirszfelda
Position
  • Research
Description
  • Stem Cells research
April 2018 - December 2019
University of Miami Miller School of Medicine
Position
  • PostDoc Position
May 2015 - April 2018
Institute of Human Genetics Polish Academy of Sciences
Position
  • Researcher
Description
  • Neurodegenerative Diseases

Publications

Publications (14)
Article
Full-text available
Amyotrophic lateral sclerosis (ALS) is a progressive motor neuronal disorder characterized by neuronal degeneration and currently no effective cure is available to stop or delay the disease from progression. Transplantation of murine glial-restricted precursors (mGRPs) is an attractive strategy to modulate ALS development and advancements such as t...
Article
Full-text available
Current treatment protocols for myocardial infarction improve the outcome of disease to some extent but do not provide the clue for full regeneration of the heart tissues. An increasing body of evidence has shown that transplantation of cells may lead to some organ recovery. However, the optimal stem cell population has not been yet identified. We...
Article
Full-text available
The studies of psychedelics, especially psychedelic tryptamines like psilocybin, are rapidly gaining interest in neuroscience research. Much of this interest stems from recent clinical studies demonstrating that they have a unique ability to improve the debilitating symptoms of major depressive disorder (MDD) long‐term after only a single treatment...
Article
Full-text available
Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease, causing motor neuron and skeletal muscle loss and death. One of the promising therapeutic approaches is stem cell graft application into the brain; however, an immune reaction against it creates serious limitations. This study aimed to research the efficiency of glial re...
Preprint
Full-text available
Psychedelics are new, promising candidate molecules for clinical use in psychiatric disorders such as Treatment-Resistant Depression (TRD) and Post Traumatic Stress Disorder (PTSD). They were recently also proposed as molecules supporting neural tissue repair by anti-inflammatory properties. Here we reported that two classic psychedelics, DMT and p...
Article
Full-text available
Duchenne muscular dystrophy (DMD) is a genetic disorder associated with a progressive deficiency of dystrophin that leads to skeletal muscle degeneration. In this study, we tested the hypothesis that a co-transplantation of two stem/progenitor cell populations, namely bone marrow-derived mesenchymal stem cells (BM-MSCs) and skeletal muscle-derived...
Article
BACKGROUND: Mesenchymal stromal/stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs from different tissue exhibit many common characteristics, their biological activity and some markers are differ...
Article
Full-text available
Neurodegeneration can be defined as a process in which neuronal structures and functions undergo changes leading to reduced neuronal survival and increased cell death in the central nervous system (CNS). Neuronal degeneration in specific regions of the CNS is a hallmark of many neurodegenerative disorders, and there is reliable proof that neural st...
Article
Full-text available
Muscular dystrophies represent a group of diseases which may develop in several forms, and severity of the disease is usually associated with gene mutations. In skeletal muscle regeneration and in muscular dystrophies, both innate and adaptive immune responses are involved. The regenerative potential of mesenchymal stem/stromal cells (MSCs) of bone...
Article
Full-text available
Active cellular transporters of harmful agents—multidrug resistance (mdr) proteins—are present in tumor, stem and endothelial cells, among others. While mdr proteins are broadly studied in tumor cells, their role in non-tumor cells and the significance of their action not connected with removal of harmful xenobiotics is less extensively documented....
Data
Representative histograms of multidrug resistance protein expression evaluated by flow cytometry methods. White cells—black histogram, isotypic control—red histogram, MDR protein expression—green histogram. (PDF)
Data
Raw data. Raw data obtained for mdr protein expression and function evaluated by flow cytometry methods. (XLS)
Data
Representative histograms of the multidrug resistance assays’ results. Unstained cells—black histogram, inhibitor treated stained cells—red histogram, stained control cells—green histogram. Multidrug resistance activity factors [%] are shown in each graph. (PDF)
Article
Full-text available
Multipotent mesenchymal stromal/stem cells (MSCs) reside in many human organs and comprise heterogeneous population of cells with self-renewal ability. These cells can be isolated from different tissues, and their morphology, immunophenotype, and differentiation potential are dependent on their tissue of origin. Each organ contains specific populat...

Questions

Questions (2)
Question
Hello, friends!
I am very exciting to start my journey in microglia research. This is my first small independent project and first encounter with microglia in vitro isolation and culture. I am preparing to buy reagents, therefore I have some questions about what to expect in in vitro culture.
I plan to isolate microglia from adult mouse brain using MACS for CD11b and then also confirm isoation with IBA1 staining. I will be using DMEM 10% FBS, 1% penicillin/streptomycin, 2 mM l-glutamine as culture media and perhaps add TGF-B to promote proliferation. Does it sound like a good isolation protocol?
I also am curios about how long can I culture them? and if they can be passaged or freezed without risk of losing their resting state or the potential to be activated with LPS?
Do you have some experiences or special tips that you could share with me, that could help me in best performance? Every information will be priceless, because I really do have no experience in microglia right now :)
Thank you very much!
Question
Hello friends,
I had been working with EnVision™ G|2 System/AP, Rabbit/Mouse (Permanent Red) kit for years and got great results in paraffin embedded slides (IHC), however due to some changes in company policy, I have to change this kit for something else. Do you know maybe some good alternative to it? Would be nice to have some tips from more experienced friends, than trying multiple kits by myself :)
I am interested in application of AP system, in IHC of paraffin embedded sections of human tissues. I will be using mostly rabbit or mouse antibodies, after antigen exposure in buffer. I will be sometimes looking for single, characteristic cells which resembles looking for a needle in haystack, so good standardization of the kit, less background and high trust in quality would be very important to me :)
I would highly appreciate your help!
Best
Urszula

Network

Cited By

Projects

Projects (3)
Project
Investigation if psilocybin may help prevent SCA3 progression and keep the BBB integrity (preclinical study).
Project
In vitro study on the role of magic mushroom alkaloid - psilocybin and ayahuasca compound - DMT on neuroimmunology of the CNS. I wonder if it might boost regeneration and maintenance of local anti-inflammatory environment.
Project
Evaluation of biological properties of Mesenchymal Stem Cells from four distinct niches in human body.