Umberto Galderisi

Umberto Galderisi
Università degli Studi della Campania "Luigi Vanvitelli · Dipartimento di Medicina Sperimentale

Master of Science

About

33
Publications
11,662
Reads
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1,704
Citations
Citations since 2016
12 Research Items
1644 Citations
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2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
Introduction
Main current research interests • Basic and applied researches on normal and cancer stem cells. • Analysis of senescence processes that affect stem cell properties. • Effect of low dose radiations on the biology of normal and cancer stem cells.
Additional affiliations
December 2018 - January 2021
Università degli Studi della Campania "Luigi Vanvitelli
Position
  • Professor (Full)
Description
  • I am professor of molecular biology at School of Medicine of Luigi Vanvitelli in Naples, Italy. I am Adjunct Professor at Sbarro Research Institute of Temple University, Philadelphia, PA, USA I am Senior Research Consultant at GENKOK Stem Cell Research Center of Erciyes University, Kayseri, Turkey

Publications

Publications (33)
Article
Full-text available
Senotherapeutics are new drugs that can modulate senescence phenomena within tissues and reduce the onset of age-related pathologies. Senotherapeutics are divided into senolytics and senomorphics. The senolytics selectively kill senescent cells, while the senomorphics delay or block the onset of senescence. Metformin has been used to treat diabetes...
Book
Full-text available
Il nuovo testo di Biologia e Tecniche della Riproduzione edito da Edi.Ermes è diretto agli studenti dei corsi di laurea in Biologia e Biotecnologie, ma anche dei Master e dei Corsi di perfezionamento e ai professionisti operanti nell’ambito della riproduzione umana. Nella prima sezione sono sviluppati argomenti relativi alla biologia della riproduz...
Article
Full-text available
The mesenchymal stromal cells (MSCs) residing within the stromal component of visceral adipose tissue appear to be greatly affected by obesity, with impairment of their functions and presence of senescence. To gain further insight into these phenomena, we analyzed the changes in total proteome content and secretome of mouse MSCs after a high-fat di...
Preprint
Full-text available
Senescent cells secrete several molecules, collectively named senescence-associated secretory phenotype (SASP). In the SASP of cells that became senescent following several in vitro chemical and physical stress, we identified the IGFBP-4 protein that can be considered a general stress mediator. This factor appeared to play a key role in senescence-...
Article
Full-text available
Curcumin, a nontoxic, naturally occurring polyphenol, has been recently proposed for the management of neurodegenerative and neurological diseases. However, a discrepancy exists between the well-documented pharmacological activities that curcumin seems to possess in vivo and its poor aqueous solubility, bioavailability, and pharmacokinetic profiles...
Article
Full-text available
Metabolic flexibility describes the ability of cells to respond or adapt its metabolism to support and enable rapid proliferation, continuous growth, and survival in hostile conditions. This dynamic character of the cellular metabolic network appears enhanced in cancer cells, in order to increase the adaptive phenotype and to maintain both viabilit...
Article
The use of high-linear energy transfer charged particles is gaining attention as a medical tool because of the emission of radiations with an efficient cell-killing ability. Considerable interest has developed in the use of targeted alpha-particle therapy for the treatment of micrometastases. Moreover, the use of helium beams is gaining momentum, e...
Article
Rett syndrome (RTT) is a neurodevelopmental disease that leads to intellectual deficit, motor disability, epilepsy and increased risk of sudden death. Although in up to 95% of cases this disease is caused by de novo loss-of-function mutations in the X-linked methyl-CpG binding protein 2 gene, it is a multisystem disease associated also with mitocho...
Article
MicroRNAs are able to modulate gene expression in a range of diseases. We focused on microRNAs as potential contributors to the pathogenesis of ascending aorta (AA) dilatation in patients with stenotic tricuspid (TAV) or bicuspid aortic valve (BAV). Aortic specimens were collected from the ‘concavity’ and the ‘convexity’ of mildly dilated AAs and o...
Article
Full-text available
Understanding the mechanisms by which mesenchymal stromal cells (MSCs) interact with the physical properties (e.g. topography, charge, ζ-potential, and contact angle) of polymeric surfaces is essential to design new biomaterials capable of regulating stem cell behavior. The present study investigated the ability of two polymers (pHM1 and pHM3) with...
Article
Full-text available
Changes in epigenetic marks are known to be important regulatory factors in stem cell fate determination and differentiation. In the past years, the investigation of the epigenetic regulation of stem cell biology has largely focused on embryonic stem cells (ESCs). Contrarily, less is known about the epigenetic control of gene expression during diff...
Poster
Full-text available
Senesence is a degenerative progress in which cells stops cell dividing yet these cells continue their metabolic activity. Mesenchymal stem cells (MSCs) exist in many tissues and play an important role in the regulation of cells in a niche with their secreted molecules. In this study; conditional media (CM) which includes secretome was collected fr...
Poster
Full-text available
Senescence is a cellular aging mechanism which causes loss of function in the cell, reduces regenerative feature of tissue and can either inhibit or promote the tumor growth. Senescence occurs in two ways. First, depends on elapsed time in culture that is called replicative or chronic senescence. Second, depends on acute senescence which can be ind...
Article
Full-text available
In the last year, the promising features of mesenchymal stem cells (MSCs), including their regenerative properties and ability to differentiate into diverse cell lineages, have generated great interest among researchers whose work has offered intriguing perspectives on cell-based therapies for various diseases. Currently the most commonly used adul...
Conference Paper
Introduction Mesenchymal stromal cells (MSCs) differentiate in mesodermal derivatives, support hematopoiesis, contribute to the homeostatic maintenance of many organs and tissues and modulate inflammatory response. All these activities are accomplished mainly by secretion of a plethora of cytokines and growth factors. MSC secretome is profoundly af...
Article
BACKGROUND SOX2 is one of the pluripotency transcription factors expressed by stem cells, which plays a central role in controlling the expression of genes implicated in embryonic development and stemness manteinance. Key regulators of embryonic stem cell (ESC) identity, such as NANOG, SOX2, OCT4 and GDF3 resulted overexpressed in a variety of soli...
Article
Full-text available
Low doses of radiation may have profound effects on cellular function. Individuals may be exposed to low doses of radiation either intentionally for medical purposes or accidentally, such as those exposed to radiological terrorism or those who live near illegal radioactive waste dumpsites. We studied the effects of low dose radiation on human bone...
Article
Restenosis is pathophysiological process occurring in 10-15% of patients submitted to revascularization procedures of coronary, carotid and peripheral arteries. It can be considered as an excessive healing reaction of the vascular wall submitted to arterial/venous bypass graft interposition, endarterectomy or angioplasty. The advent of bare metal s...
Article
Several investigators have cultivated marrow stromal cells and have identified a population of mesenchymal stem cells (MSCs). These cells expand extensively in vitro and exhibit multilineage differentiation potential. The lack of MSC-specific markers impedes identification of MSC functions. Further in vivo studies of these cells may elucidate the n...
Article
Full-text available
The events leading to breast cancer (BC) progression or recurrence are not completely understood and new prognostic markers aiming at identifying high risk-patients and to develop suitable therapy are highly demanded. Experimental evidences found in cancer cells a deregulated expression of some genes involved in governance of stem cell properties a...
Article
The diagnosis of glioblastoma is still based on tumor histology, but emerging molecular diagnosis is becoming an important part of glioblastoma classification. Besides the well-known cell cycle-related circuitries that are associated with glioblastoma onset and development, new insights may be derived by looking at pathways involved in regulation o...
Article
Full-text available
Bone marrow adipose tissue (BMAT) is different from fat found elsewhere in the body, and only recently have some of its functions been investigated. BMAT may regulate bone marrow stem cell niche and plays a role in energy storage and thermogenesis. BMAT may be involved also in obesity and osteoporosis onset. Given the paramount functions of BMAT, w...
Book
Complex physiopathological relationships have been proven to exist between two of the body's most vital organs; the brain and the heart. In Cell Cycle Regulation and Differentiation in Cardiovascular and Neural Systems Antonio Giordano, Umberto Galderisi and a panel of the most respected authorities in their field offer an in-depth analysis of the...

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Projects

Projects (7)
Project
In adulthood, tissue-specific stem cells regulate homeostatic tissue regeneration. Stem cells are located in specific areas of tissues, called niches, and are characterized as being in a state of relative proliferative quiescence, from which they can exit under the proper conditions to obtain the proliferative potential necessary for tissue regeneration. Stem cells reside for long periods of time in our bodies, and this increases the possibility that they may be subjected to genotoxic damage, which may derive from extrinsic (ionizing radiation, drugs, chemicals, etc.) or intrinsic sources (DNA replication errors, spontaneous chemical changes to DNA, programmed DNA recombination). Following damage, cells may properly repair DNA and restore functionality, or they may accumulate irreversible damages that trigger either apoptosis or senescence. Alternatively, damaged cells may undergo transformation with the onset of cancer. Mesenchymal stromal cells (MSCs) are present in the stroma of several organs and tissues, such as bone marrow, adipose tissue, dental pulp, and umbilical cord. MSCs are not a homogenous population but comprehend several cell types, such as stem cells, progenitor cells, fibroblasts, and other types of cells. Stem cells present in an MSC population can differentiate into mesodermal lineage cells (adipocytes, chondrocytes, osteocytes, and muscle cells) but also in cells belonging to endodermal and ectodermal lineages, at least in vitro. For this reason, several researchers proposed that MSCs may contain a subpopulation of pluripotent stem cells. Indeed, in the past, several authors have identified putative pluripotent stem cells in MSCs, such as multipotent adult progenitor cells (MAPCs) or very small embryonic stem cells (VSELs). Many scientists questioned the existence of these cells. In recent years, the Dezawa's research group identified a population of pluripotent stem cells, which represent around 1-3% of MSCs. These cells were named multilineagedifferentiating stress enduring (Muse) cells since they were found to be stress-tolerant cells. Muse cells express the pluripotent surface marker SSEA-3 and other pluripotency genes (NANOG, OCT-3/4, SOX2). They can differentiate into triploblastic cells from a single cell and are self-renewable. In MSC cultures, other cell types do not possess the properties of Muse cells. Indeed, Muse cells, isolated from a heterogeneous stromal cell culture, can differentiate into functional melanocytes, while non-Muse cells fail to do so. In an animal model of stroke, Muse cells can replenish lost neurons and contribute to pyramidal tract reconstruction. Muse cells can also differentiate into liver cells when intravenously injected into animals that were subjected to hepatectomy. All these studies indicate that Muse cells are pluripotent, but non-Muse cells in MSC cultures are not.
Project
Senescence-associated secreted proteins (SASP) In the last years, I have focused on the secretome of senescent cells. It is evident that senescence process may greatly affect also the composition of mesenchymal stem cells (MSC) secretome through a shift from functional paracrine signalling to production of senescent-associated secreted factors that have potent autocrine and paracrine activities. Changes in secretome profiles of MSC may great impair their activities, which depends on the capability to secrete many factors, like cytokines and chemokines. In this setting, we have decided to carry out a comparative analysis of MSC secretome from young and senescent cultures and evaluate if factors secreted from old MSC cultures may induce senescence or arrest proliferation, or promote cytotoxic effects in young cells.
Project
Rett syndrome (RTT) is one of the most common genetic causes of mental retardation in young females. Despite a large amount of data on the role of Mecp2 as the causative gene in RTT, several questions remain unanswered. In fact, it is difficult to reconcile the major neurological symptoms of RTT pathology with the widespread expression pattern of Mecp2 and its promiscuous binding to chromosomes. Because RTT is a neurodevelopmental disease, researches primarily focused with respect to neuronal functions. Consequently, more subtle deficiencies, unrelated to neural activities and/or the impairment of cell functions outside the nervous system, may have been overlooked. MECP2 is a member of a family of proteins that preferentially bind to methylated CpGs and the DNA methylation is an epigenetic modification that occurs almost exclusively in the context of CpG dinucleotides. Taking into account both the observation that Mecp2-impaired function may affect several organs apart from neural systems and the role of Mecp2 as a global regulator of gene expression, we have studied the in vitro behaviour of mesenchymal stem cells (MSCs) obtained from RTT patients in comparison with cells from healthy donors. We have RTT patients' MSCs because they are progenitors of osteocytes, and it has been suggested that RTT patients' osteogenesis could be impaired. Moreover, MSCs play a key role in the homeostasis of many organs and tissues. We found that senescence phenomena affect the biology of MSCs obtained from RTT patients and from RTT mouse model. Currently, we are investigating in depth the role of Mecp2 in the senescence of MSCs and other stem cell types, such as neural stem cells and satellite muscle cells.