Ulrike Boehm

Carl Zeiss AG · Corporate Research & Technology

By enlarging the aperture along the optic axis, the coherent utilization of opposing objective lenses (4Pi arrangement) has the potential to offer the sharpest and most light-efficient point-spread-functions in three-dimensional (3D) far-field fluorescence nanoscopy. However, to obtain unambiguous images, the signal has to be discriminated against...

A study was conducted to demonstrate flexible microdomain specific staining of block copolymers (BCP) for 3D optical nanoscopy. Homopolymers were tagged by incorporation of reactive functional groups during synthesis that were reacted with complementarily functionalized dyemolecules. Two variants of two common click chemistry reactions were used, i...

STED microscopy is an emerging method in the characterization of block copolymer film morphologies. We demonstrate a complete experimental platform that allows us to obtain in situ Three Dimensional images with microdomain specificity. IsoSTED, a variant of STED microscopy that coherently combines two opposing lenses, yields the necessary resolutio...

A principal limitation of cryo-transmission electron microscopy performed on cells or tissues is the accessible specimen thickness. This is exacerbated in tomography applications, where the aspect ratio (and thus the apparent specimen thickness) changes considerably during specimen tilting. Cryo-ultramicrotomy is the most obvious way of dealing wit...

Inside the cell, proteins essential for signaling, morphogenesis, and migration navigate complex pathways, typically via vesicular trafficking or microtubule-driven mechanisms 1–3 . However, the process by which soluble cytoskeletal monomers maneuver through the cytoplasm’s ever-changing environment to reach their destinations without using these p...

Together with the molecular knowledge of genes and proteins, biological images promise to significantly enhance the scientific understanding of complex cellular systems and to advance predictive and personalized therapeutic products for human health. For this potential to be realized, quality-assured image data must be shared among labs at a global...

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microsco...

To obtain accurate, reproducible, and interpretable data when conducting imaging experiments, it is critical to consider external factors affecting data acquisition at various steps of the experimental workflow. Illumination power and stability represent two critical factors, especially when comparing fluorescence intensities between images during...

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images and image analyses results, there are to date no unified guidelines. Consequently, microscopy images and imag...

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However for scientists wishing to publish the obtained images and image analyses results, there are to date no unified guidelines. Consequently, microscopy images and imag...

Fluorescence microscopy images should not be treated as perfect representations of biology. Many factors within the biospecimen itself can drastically affect quantitative microscopy data. Whereas some sample-specific considerations, such as photobleaching and autofluorescence, are more commonly discussed, a holistic discussion of sample-related iss...

Rigorous record-keeping and quality control are required to ensure the quality, reproducibility and value of imaging data. The 4DN Initiative and BINA here propose light Microscopy Metadata Specifications that extend the OME Data Model, scale with experimental intent and complexity, and make it possible for scientists to create comprehensive record...

For quality, interpretation, reproducibility and sharing value, microscopy images should be accompanied by detailed descriptions of the conditions that were used to produce them. Micro-Meta App is an intuitive, highly interoperable, open-source software tool that was developed in the context of the 4D Nucleome (4DN) consortium and is designed to fa...

To obtain accurate, reproducible, and interpretable data when conducting imaging experiments, it is critical to consider external factors affecting data acquisition at various steps of the experimental workflow. Illumination power and stability represent two critical factors, especially when comparing fluorescence intensities between images during...

In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality c...

In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality c...

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated, quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing a...

For the information content of microscopy images to be appropriately interpreted, reproduced, and meet FAIR (Findable Accessible Interoperable and Reusable) principles, they should be accompanied by detailed descriptions of microscope hardware, image acquisition settings, image pixel, and dimensional structure, and instrument performance. Nonethele...

The community-driven initiative Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy image data through quality control (QC) management of instruments and images. It aims for a common set of QC guidelines for hardware calibration and image acquisition...

1 - ABSTRACT Digital light microscopy provides powerful tools for quantitatively probing the real-time dynamics of subcellular structures. While the power of modern microscopy techniques is undeniable, rigorous record-keeping and quality control are required to ensure that imaging data may be properly interpreted ( quality ), reproduced ( reproduc...

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated, quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing a...

Resolving the 3D Nano-architecture of the Actin Cytoskeleton - James Galbraith, Jesse Aaron, Ulrike Boehm, Teng-Leong Chew, Catherine Galbraith

I, Scientist is a conference for students and young researchers in the natural sciences and mathematics. We aim to present female role models, unfold the diversity of career possibilities, and raise awareness of the inequalities still persisting between the careers of men and women. Few people are aware that even excellently educated women have to...

tsCOX8a-Dronpa-M159T, targeted to mitochondria. Fixed-cell recording, 14.06 x 4.00 x 10.94 μm3 (xzy).

Supplementary Figures 1-5, Supplementary Table 1, Supplementary Methods and Supplementary References.

Lifeact-Dronpa-M159T, targeted to actin. Live-cell recording, 28.13 x 6.25 x 4.00 μm3 (xzy).

Vimentin-Dronpa-M159T. Prior to acquisition, three sample regions (centered at the medial xz-plane of the recorded volume) were deliberately exposed to coincident illumination of the activation and read-out light, which negated the bleaching protection introduced by the 2-photon-activation pulse scheme and consequently resulted in a local signal at...

Die Vergrößerung des Öffnungswinkels entlang des optischen Achse eines Mikroskopes durch die kohärente Überlagerung der Foki zweier sich gegenüberstehender Objektive (4Pi-Anordnung) ermöglicht die Erzeugung der lichteffizientesten und schärftesten Punktbildfunktionen, um die dreidimensionale Auflösung in der Fernfeldmikrokopie weit unter die Beugun...

of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.