Twana Alkasalias

Twana Alkasalias
Karolinska Institutet | KI · Department of Microbiology, Tumor and Cell Biology (MTC)

Ph.D. in Cell & Tumor Biology

About

15
Publications
2,622
Reads
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328
Citations
Additional affiliations
February 2013 - present
Karolinska Institutet
Position
  • PhD

Publications

Publications (15)
Article
Purpose: Cancer metastasis can occur at the early stage of tumor development when a primary tumor at the microscopic size. In particular, the interaction of malignant cells with other cell types including cancer-associated fibroblasts (CAFs) in promoting metastasis at the early stage of tumor development remains largely unknown. Here, we investigat...
Article
Full-text available
Fibroblasts are a main player in the tumor-inhibitory microenvi-ronment. Upon tumor initiation and progression, fibroblasts can lose their tumor-inhibitory capacity and promote tumor growth. The molecular mechanisms that underlie this switch have not been defined completely. Previously, we identified four proteins over-expressed in cancer-associate...
Article
Full-text available
There is growing evidence that emerging malignancies in solid tissues might be kept under control by physical intercellular contacts with normal fibroblasts. Here we characterize transcriptional landscapes of fibroblasts that confronted cancer cells. We studied four pairs of in vitro and ex vivo fibroblast lines which, within each pair, differed in...
Article
Full-text available
Normal human and murine fibroblasts can inhibit proliferation of tumor cells when cocultured in vitro. The inhibitory capacity varies depending on the donor and the site of origin of the fibroblast. We showed previously that effective inhibition requires formation of a morphologically intact fibroblast monolayer before seeding of the tumor cells. H...
Article
Full-text available
Tumorigenesis is a complex process involving dynamic interactions between malignant cells and their surrounding stroma, including both the cellular and acellular components. Within the stroma, fibroblasts represent not only a predominant cell type, but also a major source of the acellular tissue microenvironment comprising the extracellular matrix...
Preprint
Full-text available
Highly specific and potent inhibitors of dihydroorotate dehydrogenase (DHODH), an essential enzyme of the de novo pyrimidine ribonucleotide synthesis pathway, are in clinical trials for autoimmune diseases, viral infections and cancer. However, because DHODH inhibitors (DHODHi) are immunosuppressants they may reduce the anticancer activity of the i...
Article
Full-text available
Nitric oxide, carbon monoxide and hydrogen sulfide are three endogenous gasotransmitters that serve a role in regulating normal and pathological cellular activities. They can stimulate or inhibit cancer cell proliferation and invasion, as well as interfere with cancer cell responses to drug treatments. Understanding the molecular pathways governing...
Article
Full-text available
Coronavirus disease-19 (COVID-19) is a complex disease that causes illness ranging from mild to severe respiratory problems. It is caused by a novel coronavirus SARS-CoV-2 (Severe acute respiratory syndrome coronavirus-2) that is an enveloped positive-sense single-stranded RNA (+ssRNA) virus belongs to coronavirus CoV family. It has a fast-spreadin...
Conference Paper
Despite increased understanding of chemotherapy resistance, the effects of the chemotherapy itself on the oncogenic cancer cell properties and adaptive resistance mechanisms of the therapy-escaping metastatic cancer cells remain largely undefined. In this study, we show that the platinum-based chemotherapeutic drugs cisplatin and carboplatin induce...
Data
Video S1 Representative time‐lapse imaging on the migration of endometrial stromal cells recorded for 24 hrs. The experiment was performed in three healthy volunteers.
Data
Figure S1 A. Representative micrographs of undifferentiated stromal cells and cells in response to MPA (1 μM), db‐cAMP (0.5 mM) and their combined treatment after 6 days were taken using an inverted microscope with 40× magnification. B. Relative gene expression levels of PRL, IGFBP1 and PROK1 based on three healthy volunteers in response to MPA (1...
Data
Video S2 Representative time‐lapse imaging on the migration of endometrial stromal cells in the presence of 50 ng/ml PROK1 recorded for 24 hrs. The experiment was performed in three healthy subjects.
Article
Full-text available
Prokineticin 1 (PROK1), a hypoxia-regulated angiogenic factor, has emerged as a crucial regulator of embryo implantation and placentation. Dysregulation of PROK1 has been linked to recurrent pregnancy loss, pre-eclampsia, foetal growth restriction and preterm birth. These pregnancy complications are common in women with obesity and polycystic ovary...
Conference Paper
Full-text available
Phosphodiesterase-5 (PDE5) inhibitors showed a vasodilator effect on precontracted isolated rat smooth muscles through increasing the half life of cyclic nucleotides cyclic, guanosine monophosphate (cGMP). The level of cGMP can be modulated by Phosphodiesterase isoenzymes, mainly by PDE5. Sildenafil reduced the contractile tone of vas deferens by 3...
Conference Paper
Phosphodiesterase-5 (PDE5) inhibitors showed a vasodilator effect on precontracted isolated rat smooth muscles through increasing the half life of cyclic nucleotides cyclic, guanosine monophosphate (cGMP). The level of cGMP can be modulated by Phosphodiesterase isoenzymes, mainly by PDE5. Sildenafil reduced the contractile tone of vas deferens by 3...

Questions

Question (1)
Question
i have transduced normal human fibroblasts with lentivirus-CRISPR/cas system, then the clonal selection was performed after puro selection. couple of the selected clones showed completely KO phenotype, while some clones did not show any change on protein level.
the question is: can we consider the clone which did not show a knock-out phenotype as a control to the KO clones?
on other hand i would like to get suggestions about selecting clones after the lentiviral transduction. Is it good or bad idea? Alternatively, shall we go for a pool of clones ?

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