Tushar Ranjan

• Ph.D
• Assistant Professor-cum-Scientist (PG) at Bihar Agricultural University

Nitric oxide has been shown to influence oxidative metabolism in plants, enhancing their resilience to various biotic and abiotic stresses. Post‐harvest oxidative stress is a key factor leading to quality deterioration in litchi ( Litchi chinensis Sonn.) fruit, with visible symptoms that significantly reduce shelf life and consumer acceptability. T...

An invasive alien pest, fall armyworm has been recently introduced to India. To date, two strains of FAW have been documented, viz. R strain (rice) and C strain (corn) without any clear biological attributes, even though differences are evident. A survey was conducted during 2020–22 over 18 field visits across the eastern part of India (Bihar, West...

Chickpea is a highly nutritious protein-rich source and one of the major crops to alleviate global malnutrition, but poor seed quality affects its productivity. Seed quality is essential for better crop establishment and higher yields, particularly in the uncertain climate change. The present study investigated the impact of botanical priming versu...

In the original publication [...]

Plants contain a wide range of naturally occurring compounds that have biological relevance by assisting plants in defending themselves against various plant diseases by exhibiting antifungal, antimicrobial compounds through inhibition or killing processes. Synthetic antimicrobial chemicals have become widely employed in plants in preventing and cu...

Papaya ringspot virus (PRSV), which is transmitted by aphids, is considered to be the major virus infecting papaya and cucurbits worldwide. In this study, we detected the virus and other associated pathogen complexities in papaya and cucurbits through polymerase chain reaction (PCR) assay. In one step reverse transcription (RT)-PCR tests, primarily...

Chickpea is a highly nutritious protein-rich source and one of the major crops to alleviate global malnutrition. It is the third-largest legume cultivated globally in arid and semi-arid environments. Chickpea productivity is affected by several factors, among which poor seed quality is one of the significant factors contributing to its decrease in...

Phosphate solubilizing bacteria (PSB) is well-known to promote crop growth and yield. But the isolation of PSB, characterization and evaluation of solubilization capacity from insoluble phosphatic sources are rarely conducted from subtropical soils. Thus in an incubation study total seven bacterial isolates including two check isolated from subtrop...

It is about molecular approach for plant disease management

The decreased availability of phosphorus (P) in Indian soils including the soils of Bihar can be attributed to its affinity to the formation of complex compounds. This study is undertaken to investigate the interactive effect of mineral P, nano P and phosphate-solubilizing bacteria (PSBs) for different inorganic and organic P fractions in an alluvi...

Litchi fruit is valued for its characteristic flavor and attractive color. However, oxidative pericarp browning soon after harvest limits its shelf-life and availability. Melatonin has been reported to mitigate several abiotic stresses in plant and plant based products. In this investigation, the effects of postharvest application of melatonin (0.1...

Genome packaging is the crucial step for maturation of plant viruses containing an RNA genome. Viruses exhibit a remarkable degree of packaging specificity, despite the probability of co-packaging cellular RNAs. Three different types of viral genome packaging systems are reported so far. The recently upgraded type I genome packaging system involves...

Northern corn leaf blight (NCLB) of maize, caused by Exserohilum turcicum (Pass.) Leonard and Suggs., is an important foliar disease common across maize-producing areas of the world, including Bihar, India. In this study, virulence and distribution of races were observed against Ht-resistant genes and also identified the E. turcicum race population...

Genome packaging is a critical step during the viral maturation process. Viruses employ a distinct approach to package their genetic materials inside capsid: ranging from very simple strategy of nucleation of capsid proteins onto genome to complex segro-packasome machinery. The majority of small plant viruses, which falls under type I passive syste...

Maize is considered the third most important cereal crop in Asia after rice and wheat. Many diseases affect this crop due to the cultivation of various hybrids. This research aimed to characterize the causative agent of northern corn leaf blight disease in Bihar, India, caused by Ex-serohilum turcicum (Passerini) Leonard and Suggs. Leaf samples wer...

Safflower (Carthamus tinctorius L.) is known for its oil quality and ability to grow in drought conditions. The threat to global food security posed by challenges of climate change has stressed the use of functional genomics in current plant breeding approaches. Plant steroid hormones called brassinosteroids (BRs) are at the nexus of regulating pla...

Potato leafroll virus (PLRV) uses powerful molecular machines to package its genome into a viral capsid employing ATP as fuel. Although, recent bioinformatics and structural studies have revealed detailed mechanism of DNA packaging, little is known about the mechanochemistry of genome packaging in small plant viruses such as PLRV. We have identifie...

Thirteen lines and three testers were used to produce 39 single cross maize hybrids by line Ítester mating design. The genetic divergence among thirteen lines and three tester of maize were estimated by using Mahalanobis D2 statistic for twelve characters. The genotypes were grouped into five clusters. Cluster I comprised 12 parental genotypes (L1,...

Geminiviruses infect many crop plants, and are limiting factors for veg-etable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for begomo-virus, virus DNA was extracted and charac...

An experiment was conducted for the study of heterosis and combining ability in Indian mustard, (Brassica juncea L.) using 7×7 half diallel mating design along with their parents. Data were recorded for eleven quantitative characters. Analysis of variance (ANOVA) of design of experiment showed significant differences between all the treatments for...

Investigation was carried out to ascertain the genetic architecture for heat tolerance and yield components from diallel crosses in maize (Zea mays L.). The combining ability in both the normal and heat stress conditions revealed highly significant mean squares due to general combining ability (GCA) and specific combining ability (SCA) in both the...

Geminiviruses infect many crop plants, and are limiting factors for vegetable crop production. Begomoviruses (Geminiviridae) cause typical symptoms of leaf curling and puckering in nightshade (Solanum nigrum), a seasonal weed in Bihar, India. To investigate if nightshade was an intermediate host for begomovirus, virus DNA was extracted and characte...

Potato leaf roll virus (PLRV) uses powerful molecular machines to package its genome into a viral capsid employing ATP as fuel. Although, recent bioinformatics and structural studies have revealed detailed mechanism of DNA packaging, little is known about the mechanochemistry of genome packaging in small plant viruses such as PLRV. We have identifi...

Plant viruses cause various disease in crops and are responsible for huge economic losses. Understanding their mode of infection and spread is crucial for developing control strategies. Genome packaging is an important step in the process of viral maturation. Three systems of viral genome packaging are known till date. The type I packaging system,...

The host range of a virus is defined as the number of species a virus potentially infects. The specialist virus infects one or few related species while the generalist virus infects several different species, possibly in different families. Origin of generalist viruses from their specialist nature and the expansion of the host range of the generali...

Viruses cause many severe plant diseases, resulting in immense losses of crop yield worldwide. Therefore, developing novel approaches to control plant viruses is crucial to meet the demands of a growing world population. Recently, RNA interference (RNAi) has been widely used to develop virus-resistant plants. Once genome replication and assembly of...

The whole world is still suffering substantially from the coronavirus disease 2019 (COVID-19) outbreak. Several protein-based molecules that are associated with the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which are essential for its functionality, survival, and pathogenesis have been identified and are considered as potential...

Objective The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results Although aphids pop...

The whole world is still suffering substantially from the coronavirus disease 2019 (COVID-19) outbreak. Several protein-based molecules that are associated with the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which are essential for its functionality, survival, and pathogenesis have been identified and are considered as potential...

Climate-smart agriculture (CSA) is an approach to developing the technical, policy and investment conditions to achieve sustainable agricultural development for food security under climate change. multiple cropping systems using crop rotations or intercropping, growing of nitrate catch crops, growing of deep rooting crops following harvest of short...

In the recent past years, global warming and climate change have drastically affected the agricultural crop productivity grown in tropical and subtropical areas globally by appearing to several new biotic and abiotic stresses. Among the abiotic stresses, heat, drought, moisture, and salt stresses are most prevalent. Wheat is the most common and wid...

Objective The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield loss worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results Although population o...

Objective: The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results: Although aphids po...

Objective: The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results: Although aphids po...

Objective: The Potato Leaf Roll Virus (PLRV) is one of the most devastating virus causing severe yield losses worldwide in potato. The comprehensive observations were made to study the PLRV infestation in major potato growing areas of Bihar (India) and further detailed molecular basis of PLRV aggravation was established. Results: Although aphids po...

Letter

To investigate the production of cellulases and hemicellulases from Aspergillus niger MTCC9931, solid state fermentation (SSF) was performed using 10 different lignocellulosic materials derived from agrowastes, i.e., rice straw, rice husk, wheat straw, corn cob, sugar cane bagasse, saw dust, banana stalk, Eichhornia, Parthenium stalk, and residual...

Antimicrobial peptides (AMPs) are the small ubiquitous self-defence products which are extensively distributed in plants. They can be classified into several groups, including thionins, defensins, snakins, lipid transfer proteins, glycine-rich proteins, cyclotides, and hevein-type proteins. AMPs are important mediators of an innate host defence sys...

Thirty well-characterized pseudomonad isolates for plant growth-promoting traits were screened for their antagonistic activities against 20 isolates of Sclerotium rolfsii. Out of the 30 pseudomonad isolates, PUR46 was found to be best against all 20 isolates of Sclerotium rolfsii, because of its unique ability to suppress the growth of mycelia as w...

Virus-induced gene silencing (VIGS) is a powerful reverse genetics technology used to unravel the functions of genes. It uses viruses as vectors to carry targeted plant genes. The virus vector is used to induce RNA-mediated silencing of a gene or genes in the host plant. The process of silencing is triggered by dsRNA molecules, the mechanism is exp...

The rate of pineapple propagation through conventional technique is quite low and time taken and that by seed is apparently hard to germinate. Non-availability of quality planting material is one of the major constraints for expansion of its cultivation area in Bihar. Keeping this in view an experiment was conducted for in vitro establishment for l...

Litchi (Litchi chinensis Sonn.), a subtropical fruit crop has high commercial value and consumer acceptance owing to its rich juicy aril and attractive bright red pericarp. Anthocyanin, the major pigment present in litchi pericarp reaches its maximum content in fully ripen fruit contributing to its bright red colour. Anthocyanin content in plants d...

Polerovirus (Family-Luteoviridae) are one of the most destructive viruses causing detrimental diseases in vegetable crops in tropical regions of the world including India. Four species viz. potato leaf roll virus (PLRV), potato virus Y(PVY), potato virus X(PVX) and potato virus S(PVS) are known to cause different diseases in potato crops. Of the va...

The study of genetic diversity among Lathyrus sativus L. may give fundamental insights into extent of genetic variation and provide options to meet the climate change challenge. 20 SSR loci were employed to assess the genetic diversity of 32 grasspea genotypes. Eleven markers proved to be polymorphic across examined genotypes in aggregation to allo...

Aims: The study aims to highlight the simple optimisation, inexpensive and rapid procedure for DNA isolation from tough leaves (Palmyra palm) without compromising the yield and purity of DNA. Study Design: Leaf of palmyra palm (Borassus flabellifer) was used to conduct the experiment followed by laboratory analysis, DNA extraction and PCR amplifica...

The present investigation was carried out using thirty eight genotypes (eight parents, twenty eight F1's and two checks) using Griffing's half diallel mating design. The experiment was laid out in a randomized complete block design with three replications during kharif 2017 at the research farm of Bihar Agriculture College, Sabour. Observations wer...

Twenty-eight crosses generated from eight diverse parents of linseed (Linum usitatissimum L.) in diallel mating design (excluding reciprocal) were evaluated against Shekhar as check-in randomized block design for nine quantitative traits, to estimate the general combining ability (GCA) of the parents and specific combining ability (SCA) of crosses,...

Drought is one of the most common environmental stresses that affect growth and development of plants. Drought can also influence plants in terms protein changes, antioxidant production, osmotic adjustment, pollen sterility, grain loss, and Abscisic acid synthesis, which are much importance for grain/seed development. The pollen development is arre...

The increasing demands for better living environments have stimulated the development of consumer products with antimicrobial properties. Several new antibacterial and antifungal agents have been introduced recently, whereas such new materials still have to fulfil the rather stringent requirements for real applications. Polyaniline is one of the mo...

The hierarchical level of genome architecture from a cellular (Virus) to cellular organisms (For ex- Human) reveals remarkable correlation between genome configuration and complexity of organisms. Genomic constitution governs the phenotypic characters of particular organism and their future fates. The discrimination between us and primitive forms o...

The term 'Global or Genome Wide' means the study of expression of thousands of genes at once to create global picture of cellular function. The study of '-omics' is important to know the structure, functions & regulation of genes, to understand evolution & relationships of genomes and to interpret interactions between the genes and its environment....

Tissue culture is an important biotechnological tool applied for crop improvement, where in vitro aseptic culture of cells, tissues, organs, or whole plant takes place under controlled nutritional and environmental conditions to generate the clones of particular plants. The controlled conditions could be nutrients, pH, temperature, and proper gaseo...

Bihar is a typical eastern state, with a large proportion of land (3.2 mha) under rice, which has an average productivity of only 2.0 t ha-1. More than half of the rice area is rainfed lowland, including flood-prone ecosystems, which are characterized by low productivity obtained under traditional systems of cultivation. Despite scientists' claims...

Wilt caused by Fusarium udum Butler, is the major constraint to pigeonpea production worldwide, which can cause yield losses up to 100% in susceptible cultivars, These toxic metabolites also known is one of the weapons used by pathogen inducing disease condition in susceptible host plants. Many pathogens are known to produce toxins both in vitro an...

Drought stress triggered by changes in the external water potential is an environmental commonplace in the life of a higher plant. The compatible osmolytes, such as proline plays an important role and act as osmoprotectants during stress. Though several attempts have been made to establish a relationship between proline accumulation and drought tol...

Genome encapsidation is an essential step in the life cycle of viruses. Viruses either use some of the most powerful ATP-dependent motors to compel the genetic material into the preformed capsid or make use of the positively charged proteins to bind and condense the negatively charged genome in an energy-independent manner. While the former is a ha...

Unlabelled: Genome packaging is a critical step in the virion assembly process. The putative ATP-driven genome packaging motor of Acanthamoeba polyphaga mimivirus (APMV) and other nucleocytoplasmic large DNA viruses (NCLDVs) is a distant ortholog of prokaryotic chromosome segregation motors, such as FtsK and HerA, rather than other viral packaging...