Tushar K. Dutta

Tushar K. Dutta
Indian Agricultural Research Institute | IARI · Division of Nematology

Ph.D.
plant-nematode interactions; bacterial toxins for pest management

About

70
Publications
64,877
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896
Citations
Additional affiliations
October 2010 - present
Indian Agricultural Research Institute
Position
  • Scientist
May 2009 - April 2010
Rothamsted Research
Position
  • PhD

Publications

Publications (70)
Chapter
Nematodes infecting plant have been reported in 18th century much before fungi, bacteria and viruses were implicated to plant diseases. Nematodes are triploblastic, pseudocoelomic, microscopic, eukaryotic, multi-cellular organisms. They are ubiquitious with unsegmented body. Plant parasitic nematodes (PPNs) bear stylet and are mostly reported to af...
Chapter
With the global shift of conventional agriculture towards sustainable food production, one of the biggest challenges associated is crop losses due to plant pathogens. The ill effect of chemical pesticides on the environment and the rapid emergence of pest resistance has necessitated the deployment of viable alternatives for pest management. RNA int...
Article
Crystal (Cry) toxins produced from the soil bacterium, Bacillus thuringiensis (Bt), have gained worldwide attention since long due to their insecticidal potential. A number of receptor proteins located on the epithelial cells of the larval midgut were shown to be crucial for Cry intoxication in different insect pests belonging to order Lepidoptera,...
Article
Full-text available
Photorhabdus bacteria secrete a repertoire of protein toxins that can kill the host insect. Among them, toxin complex (Tc) proteins have gained significant attention due to their wider conservation across the different bacterial genera. In our laboratory, a C-terminal domain of TcaB protein was characterized from P. akhurstii bacterium that conferr...
Article
Full-text available
The nematode-bacterium pair Heterorhabditis indica-Photorhabdus akhurstii is a model system to study symbiotic associations. Upon introduction into the host hemocoel by its nematode partner, the bacterium produces a number of toxins which kill the insect. Nevertheless, unlike Bt toxins majority of Photorhabdus toxins are not orally active which hin...
Article
Root‐knot nematode, Meloidogyne incognita is one of the most destructive nematodes worldwide. Essential oils (EOs) are being extensively utilized as eco‐benign bionematicides, although the precise mechanism of action remains unclear. Pogostemon cablin Benth. is well known as "Patchouli", a native to South East Asia, having huge demand due to its hu...
Article
Due to climate change, population explosion, shrinkage of arable land and reduced water availability, a paradigm shift in global crop production is occurring towards protected agriculture. This input-intensive farming provides year-round production under controlled environment and by occasionally utilizing vertical spaces for soilless cultivation....
Article
The Gram-negative bacteria Photorhabdus lives in a symbiotic relationship with the insect-pathogenic Heterorhabditis nematodes and produces numerous hydrolytic enzymes, secondary metabolites and protein toxins. Seven Photorhabdus strains were previously isolated from the Heterorhabditis nematodes collected from different geographical regions of Ind...
Chapter
The prolonged usage of Bt-cotton and -corn in India, USA and China has led to the development of Bt resistance in insects. Thus, exploration of novel protein toxins is necessary that provides greater diversity of biotoxins at disposal for pest management. Entomopathogenic nematodes have long been used as insect biocontrol agents, and the insecticid...
Article
The nematode-bacterium pair Heterorhabditis indica-Photorhabdus akhurstii is a malleable model system to investigate mutualistic relations. A number of toxins produced by P. akhurstii allow the bacterium to kill the insect host. However, a few of these heterologously expressed toxins are orally active against different insects which possibly caused...
Article
Full-text available
Rice root-knot nematode (RRKN), Meloidogyne graminicola is one of the major biotic constraints in rice-growing countries of Southeast Asia. Host plant resistance is an environmentally friendly and cost-effective mean to mitigate RRKN damage to rice. Considering the limited availability of genetic resources in the Asian rice (Oryza sativa) cultivars...
Article
Full-text available
Exploration of novel genetic resources against root-knot nematode (RKN) is necessary to strengthen the resistance breeding program in cultivated rice and iInvestigations on the role of genotype-specific root anatomy in conferring a structural barrier against nematode invasion are largely underexplored. Here, we report a highly-resistant rice germpl...
Article
Photorhabdus akhurstii is an insect–parasitic bacterium that symbiotically associates with the nematode, Heterorhabditis indica. The bacterium possesses several pathogenicity islands that aids in conferring toxicity to different insects. Herein, we constructed the plasmid clones of coding sequences of four toxin genes (pirA, tcaA, tccA and tccC; ea...
Preprint
Full-text available
Background: Rice root-knot nematode (RRKN), Meloidogyne graminicola is one of the major biotic constraints in rice-growing countries of Southeast Asia. Host plant resistance is an environmentally-friendly and cost-effective mean to mitigate RRKN damage to rice. Considering the limited availability of genetic resources in the Asian rice (Oryza sativ...
Article
Background: Txp40, a 37 kDa protein, previously characterized from the gram-negative bacterium, Photorhabdus akhurstii (symbiotically associates with insect-parasitic nematode, Heterorhabditis indica), conferred insecticidal activity against Galleria mellonella. Herein, biological activity of Txp40 was evaluated against economically important inse...
Article
Full-text available
RNA interference (RNAi)-based host-induced gene silencing (HIGS) is emerging as a novel, efficient and target-specific tool to combat phytonematode infection in crop plants. Mi-msp-1, an effector gene expressed in the subventral pharyngeal gland cells of Meloidogyne incognita plays an important role in the parasitic process. Mi-msp-1 effector is co...
Article
Photorhabdus akhurstii can produce a variety of proteins that aid this bacterium and its mutualistic nematode vector, Heterorhabditis indica to kill the insect host. Herein, we characterized (by heterologously expressing in E. coli) an open reading frame (1713 bp) of the toxin complex protein, TcaB from P. akhurstii strains IARI-SGHR2 and IARI-SGMS...
Article
Full-text available
Nematode chemosensation is a vital component of their host-seeking behaviour. The globally important phytonematode, Meloidogyne incognita, perceives and responds (via their sensory organs such as amphids and phasmids) differentially to various chemical cues emanating from the rhizosphere during the course of host finding. However, compared to the f...
Article
Full-text available
Plant-parasitic nematodes (PPNs) are considered as one of the major limiting factor in global crop production system. Due to the introduction of tighter limits in registering new biocidal molecules and a gradual phase-out of nematicides for high environmental impact, considerations for non-chemical alternatives have been realised. The biofumigation...
Article
Full-text available
Root-knot nematodes are economically important plant pathogens that cause considerable damage to agricultural crops worldwide. They secrete a range of effector proteins from their specialized gland cells to modify host cell physiology to favour parasitism of the host tissue. A deeper understanding of the roles of effectors in nematode parasitism is...
Article
Full-text available
Root-knot nematodes are economically important plant pathogens that cause considerable damage to agricultural crops worldwide. They secrete a range of effector proteins from their specialized gland cells to modify host cell physiology to favour parasitism of the host tissue. A deeper understanding of the roles of effectors in nematode parasitism is...
Article
Full-text available
Plant-parasitic, root-knot nematodes (Meloidogyne spp.) are a serious problem in agri- and horticultural crops worldwide. Understanding their complex host recognition process is essential for devising efficient and environmental-friendly management tactics. In this study, the authors report a new, simple, inexpensive, efficient, and quantitative me...
Article
Biochemical analyses of nematodes have revealed that neutral lipids (especially triglycerides) are the main source of energy reserves, which is depleted as the nematodes age. Several methodologies have been developed to visualise triglyceride-rich fat stores in plant-parasitic nematodes using non-fluorescent, lipophilic dyes, such as Oil Red O. Her...
Article
Owing to the current deficiencies in chemical control options and unavailability of novel management strategies, root-knot nematode (M. incognita) infections remain widespread with significant socio-economic impacts. Helminth nervous systems are peptide-rich and appear to be putative drug targets that could be exploited by antihelmintic chemotherap...
Article
The rice root-knot nematode, Meloidogyne graminicola, seriously impairs the growth and yield of rice which is an important staple food worldwide. The disruption of neuropeptide signalling leading to attenuation in nematode behaviour and thereby perturbed infection, offers an attractive alternative to control nematodes. In this direction, the presen...
Article
Full-text available
The complex parasitic strategy of Meloidogyne incognita appears to involve simultaneous expression of its pharyngeal gland-specific effector genes in order to colonize the host plants. Research reports related to effector crosstalk in phytonematodes for successful parasitism of the host tissue is yet underexplored. In view of this, we have used in...
Article
Full-text available
The present study was conducted to investigate the comparative parasitic ability of root-knot nematode (RKN: Meloidogyne graminicola) in putatively resistant (Suraksha) and susceptible (Pusa 1121) cultivars of rice and to analyse the relative expression level of plant defence genes upon RKN infection in these cultivars. qRT-PCR analyses of RKN-infe...
Article
Full-text available
Root-knot nematodes (RKN) cause substantial yield decline in eggplant and sustainable management options to minimize crop damage due to nematodes are still limited. A number of genetic engineering strategies have been developed to disrupt the successful plant-nematode interactions. Among them, delivery of proteinase inhibitors from the plant to per...
Article
The sophisticated parasitic tactic of sedentary endoparasitic nematodes seems to involve the simultaneous alteration of the expression of multitude of its effector genes in order to hijack the plant metabolic and developmental pathway. In concordance with this hypothesis, we have targeted some candidate effector genes of Meloidogyne incognita to un...
Article
Several nonpathogenic strains of _Fusarium oxysporum_ have been selected as potential biological control agents. Culture filtrate of _F. oxysporum_ f. sp. ciceris was extracted sequentially with ethyl acetate (EtOAc) and _n_-butanol (_n_-BuOH). Chromatographic separation of EtOAc concentrate has afforded five compounds, bikaverin (1), 3-O-methyl-8-...
Article
Volatile oil of Eupatorium adenophorum leaves was extracted and analyzed by gas chromatography–mass spectrometry (GC–MS). Sesquiterpenes were identified asmajor constituents in the essential oil. Four cadinene sesquiterpenes were isolated from ethyl acetate extract of leaves and identified spectroscopically. Chemical constituents were evaluated for...
Article
Full-text available
Rice is one of the major staple food crops in the world and an excellent model system for studying monocotyledonous plants. Diseases caused by nematodes in rice are well documented and among them, root-knot nematode (RKN), Meloidogyne graminicola, causes extensive yield decline. It is therefore necessary to identify novel sources of natural resista...
Article
The sophisticated parasitic tactic of sedentary endoparasitic nematodes seems to involve the simultaneous alteration of the expression of multitude of its effector genes in order to hijack the plant metabolic and developmental pathway. In concordance with this hypothesis, we have targeted some candidate effector genes of Meloidogyne incognita to un...
Article
The sophisticated parasitic tactic of sedentary endoparasitic nematodes seems to involve the simultaneous alteration of the expression of multitude of its effector genes in order to hijack the plant metabolic and developmental pathway. In concordance with this hypothesis, we have targeted some candidate effector genes of Meloidogyne incognita to un...
Article
The sophisticated parasitic tactic of sedentary endoparasitic nematodes seems to involve the simultaneous alteration of the expression of multitude of its effector genes in order to hijack the plant metabolic and developmental pathway. In concordance with this hypothesis, we have targeted some candidate effector genes of Meloidogyne incognita to un...
Article
The sophisticated parasitic tactic of sedentary endoparasitic nematodes seems to involve the simultaneous alteration of the expression of multitude of its effector genes in order to hijack the plant metabolic and developmental pathway. In concordance with this hypothesis, we have targeted some candidate effector genes of Meloidogyne incognita to un...
Article
The sophisticated parasitic tactic of sedentary endoparasitic nematodes seems to involve the simultaneous alteration of the expression of multitude of its effector genes in order to hijack the plant metabolic and developmental pathway. In concordance with this hypothesis, we have targeted some candidate effector genes of Meloidogyne incognita to un...
Article
Full-text available
Root-knot nematodes (Meloidogyne incognita) cause substantial yield losses in vegetables worldwide, and are difficult to manage. Continuous withdrawal of environmentally-harmful nematicides from the global market warrants the need for novel nematode management strategies. Utility of host-delivered RNAi has been demonstrated in several plants (Arabi...
Article
Full-text available
A series of twenty Schiff bases, 4-(benzylideneamino)-3-mercapto-5-pyridin-2-yl-4H-1,2,4-triazoles 6a-t, designed incorporating the picolinic acid moiety present in fusaric acid, a natural nematicide isolated from Fusarium oxysporum EF119, have been synthesized in MW-induced conditions and characterized using 1H and 13C NMR, FT-IR spectroscopy and...
Article
Full-text available
With the understanding of nematode-plant interactions at the molecular level, new avenues for engineering resistance have opened up, with RNA interference being one of them. Induction of RNAi by delivering double-stranded RNA (dsRNA) has been very successful in the model non-parasitic nematode, Caenorhabditis elegans, while in plant nematodes, dsRN...
Article
Full-text available
Meloidogyne graminicola infect graminaceous plants but have lesser tendency to infect dicotyledonous plants. Meloidogyne incognita is a pest of dicots and occasionally infects cereals. Evolutionary adaptation of these root-knot nematodes to their preferred hosts might have led to variability in their gene/protein profile which could contribute to t...
Chapter
Full-text available
With the understanding of nematode-plant interactions at the molecular level, new avenues for engineering resistance have opened up, with RNA interference being one of them. Induction of RNAi by delivering double-stranded RNA (dsRNA) has been very successful in the model non-parasitic nematode, Caenorhabditis elegans, while in plant nematodes, dsRN...
Book
Plant parasitic nematodes are gaining considerable importance worldwide due to their devastating effects on crops leading to major economic downturn.Nematodes are attracted to plant roots via soluble and gaseous attractants produced by root itself or by attendant rhizosphere microorganisms.Just how nematodes move through soil to locate plant roots...
Article
Full-text available
In the recent past, rice root-knot nematode, Meloidogyne graminicola has attained wide importance due to its potential to cause major damage in rice-wheat cropping system. It has become an emerging problem in the nurseries and upland rice along with its widespread occurrence in the deepwater and irrigated rice in the different countries of Southeas...
Article
Plant chemicals in the rhizosphere originating from root exudates or sites of previous nematode penetration can influence nematode behaviour, and a number of plant compounds, some present in root exudates, have been shown either to attract nematodes to the roots, or to result in repellence, motility inhibition, or even death. The present work was c...
Article
The rice root-knot nematode Meloidogyne graminicola normally infects rice, wheat and several other graminaceous plants. Meloidogyne incognita is a serious pest of dicotyledonous crops, although it can infect and reproduce on some cereals. This paper demonstrates and compares host recognition, development and reproduction of these two species of roo...
Article
Full-text available
It has long been recognized that chemotaxis is the primary means by which nematodes locate host plants. Nonetheless, chemotaxis has received scant attention. We show that chemotaxis is predicted to take nematodes to a source of a chemo-attractant via the shortest possible routes through the labyrinth of air-filled or water-filled channels within a...
Article
Full-text available
The cyanobacterium, Synechococcus (Anacystis) nidulans, was cultured in Bothe's medium, attaining a cell count of 10.25106cells/ml and chlorophyll content of 76.2710-5mg/ml in 15 days. The cell culture was harvested, sonicated and tested for toxicity to plant-parasitic, entomopathogenic and bacterivorous nematodes in vitro. An average per cent mo...