Molecular pathogenesis and Resistance mechanisms of Candida auris
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TRINH Phan-Canh is currently a PhD Student at Max Perutz Labs Vienna, Medical University of Vienna. Trinh does research in host-pathogen interactions and molecular mechanisms of antifungal resistance. He has studied secondary compounds from Actinobacteria and Myxobacteria as a lecturer in microbiology at the University of Medicine and Pharmacy at Ho Chi Minh City for 4 years from 2016. You can find his portfolio at phancanhtrinh.com
Acinetobacter baumannii is the leading cause of hospital-acquired infection in Vietnam. Of note, antibiotic resistance genes are significantly popular in clinical isolates of A. baumannii . Therefore, rapid identification of A. baumannii and determination of antibiotic resistance genes will help to make effective clinical decisions related to antib...
Background: Combining two groups of structures of chalcone and hydrazone on the same molecule can create new structures with a better biological effect. In this topic, some hydrazones from chalcone have been synthesized and tested for activity to contribute to diversifying groups of potential antibacterial, antifungal compounds. Objectives: Synthes...
Asteraceae species were widely applied in traditional medicines in Asian countries as sources of natural antioxidants and antimicrobial agents. This study aimed to evaluate DPPH-scavenging capacities and antimicrobial activities of nine Asteraceae species collected from Southern Vietnam. Antioxidant and antimicrobial activities were determined by s...
Antimicrobial and DPPH-scavenging assay were analyzed on nine Asteraceae species collected from Southest in Vietnam. Crude extracts from Taraxacum officinale, Chrysanthemum morifolium, Ageratum conyzoides, Tagetes erecta and essential oils from Ageratum conyzoides, Helianthus annuus, Artemisia vulgaris have antimicrobial activity on tested microorg...
In view that Actinobacteria produce various types of potential antibiotics and enzymes for pharmaceutical industry, some Actinobacteria strains were isolated from the soil samples collected around tree roots in Ho Chi Minh City and Binh Duong province and further screened for the desired bioactivities. After drying at 60 °C for 30 minutes, these so...
Background – Objectives Lichens are symbiotic systems including mycobiont and photobiont, which produce various secondary metabolites having high potential in drug discovery. The present study investigated morphology, chemical components and antimicrobial activities of methanol extract of Dirinaria applanata (Fée) D.D. Awasthi collected from Ben Tr...
Introduction: Dermatophytosis is common in hot and moist climates like Vietnam, although the infection is normally limited to superficial skin but seriously affects the quality of human life. Long duration of treatment impacts gradually on patients’ adherence, which is an opportunity to develop antifungal resistance. Objectives: Determinating speci...
Background: Bacillus subtilis KP3 which produces antioxidants and has good probiotic characteristics, was isolated by Laboratory of Pharmaceutical Biotechnology, but fermentation conditions of this strain have not been studied for producing large number of B. subtilis KP3 spores. Objectives: Fermentation conditions of B. subtilis KP3 on flask and f...
Lovastatin (mevinolin), the first hypocholesterolemic drug was approved in 1987 by Food and Drug administration (FDA), USA. At present, lovastatin is quantified by high performance liquid chromatography, in the body lovastatin is converted to β – hydroxy acid form to be active. Previous study reported that lovastatin inhibit the growth of Candida a...
I am working with gene deletion on Candida auris. I transformed my cassette constructed by Fushion PCR into C. auris. I got a lot of clones comparing negative control plates.
Now I am screening by colony PCR. My problem is the target deletion clone is so rare, it took me a lot of time.
Do you know how to improve the efficiency of homologous recombination in Candida?
Thank you very much!
I read a paper from PNAS (http://www.pnas.org/content/101/46/16222). They used a lysis buffer with high amount of PVP (6,3g/5mL). It is very viscous, I can't take it by pipet!!!
To each gram of separated cells or whole-sponge tissue ground in liquid nitrogen was added 5 ml of lysis buffer containing 100 mM Tris·HCl (pH 8), 1.4 M NaCl, 20 mM EDTA, 2 ml of CTAB solution at 55°C, 100 μl of 10% SDS, 350 μl of 100 mM diethyldithiocarbamate (DETC), 100 μl of mercaptoethanol, 6.3 g of polyvinylpyrrolidone, 10 mg of lysozyme, and 500 μg of proteinase K.
Please support me in the case. I have read a Rhizopus clinical case.
Generally, I think Rhizopus spp., Mucor spp. is Zygomycetes species, which haven't septa. However, in this picture, Rhizopus sp. have many septa.