Tri Agus Siswoyo

• Professor
• Universitas Jember

Black glutinous rice is a local pigmented rice that attracts the interest of many people due to its high nutritional value. While cultivating, black glutinous rice may experience abiotic stresses, such as drought and salinity threat. Drought and salinity may lead to oxidative stress, which leads to the overproduction of reactive oxygen species (ROS...

Plant growth-promoting rhizobacteria (PGPR) are promising candidates that enhance plant growth under stressful conditions. In this study, 10 bacterial isolates were screened for their IAA production, among them JTB1 and MT22 isolates were selected which produced high IAA levels under 10% PEG and 2% NaCl stress. The isolates showed a high capacity f...

The nutraceutical properties of the pigmented rice provide an opportunity to be widely cultivated. A tendency to grow rice on sub-optimal land such as saline soil is an alternative to support food security, but the adaptation under saline soil is still questionable. This study was performed to determine the stress resistance of black (var. Ketan Hi...

A prolonged dry season can cause drought stress and have an unfavorable impact on the growth of tomato plants, resulting in crop failure. Therefore, research is needed on the resistance of tomato varieties to drought stress. The recommended technique for drought resistance screening in tomatoes is in vitro cultivation using Polyethylene Glycol (PEG...

Quintao G, Ubaidillah M, Hartatik S, Khofifa RAN, Siswoyo TA. 2023. The morpho-physiological and gene expression of East Timor’s local rice plant (Oryza sativa) response to drought and salinity stress. Biodiversitas 24: 4548-4556. East Timor is rich in local rice varieties, such as Silaun, Ime Buci, Me Klan, Me Likan, Me Meak, Me Taek, and Umak Kle...

NaCl stress in plants affects various physiological and biochemical processes that result in reduced biomass production. This study aims to determine the aspects of morphological and physiological changes of plants with indications of the induction of secondary metabolism in survival, which is indicated by an indication of an increase in bioactive...

Pradipta S, Siswoyo TA, Ubaidillah M. 2023. Nutraceuticals and bioactive properties of local Java pigmented rice. Biodiversitas 24: 571-582. Pigmented rice is used in nutraceuticals and has health benefits, on the other side, the characterization of local pigmented rice has not been widely observed. This research aimed to determine the nutraceutica...

Diabetes mellitus prevalence in Indonesia reached 19.5 million cases, which has affected the productive age population. The indigenous people of Indonesia are blessed with the second largest biodiversity in the world, including vegetables, which are also prepared as medicaments. Vegetables are well-known as natural antioxidants which evolved in met...

Consumer awareness was increasing of food safety and food security along with the high population growth rate and increasing quality of life, nanotechnology was an important concern to be able to meet future demand for livestock products. The application of nanostructured particles has been widely explored to produce livestock products with high fo...

Melinjo plant (Gnetum gnemon L.) is one of the plants that has the potential to be developed, especially inthe tropics. Melinjo plants have a protein content of 9-10%. Reactive Oxygen Species (ROS) have increasedaccumulation in plant cells offset by increased antioxidants, which serve to protect against cell damage causedby osmotic pressure in plan...

Indonesian black rice (Oryza sativa L., Ketan Hitam-2) is pigmented rice with high potency as a nutraceutical compound, especially with its high protein content. The effect of the germination period on antioxidant activities and angiotensin I-converting enzyme (ACE-I) inhibitory of Indonesian black rice seed protein was studied to determine its pot...

Usaha untuk meningkatkan produksi tomat pada lahan tercekam genangan dapat dilakukan dengan menguji varietas -varietas tomat yang sudah beredar terhadap cekaman genangan . Uji dilakukan dengan mengamati parameter pertumbuhan dan hasil beberapa varietas tomat. Penelitian ini bertujuan untuk mengetahui toleransi beberapa varietas tomat terhadap cekam...

Jahe (Zingiber officinale) merupakan tanaman rempah dan herbal yang dimanfaatkan sebagai minuman atau campuran pada bahan pangan. Jahe memiliki nilai jual yang tinggi karena banyaknya permintaan konsumen baik untuk memenuhi kebutuhan rumah tangga maupun sebagai bahan baku perusahaan jamu dan makanan. Permasalahan utama pada budidaya jahe adalah sul...

Ciplukan (Physalis angulata L.) is one of the plants from the class of herbal plants that is good to be developed because people are starting to choose herbal treatment. The content of ciplukan fruit is phenolic and flavonoid. Thus, technology for enlargement and increasing fruit content with the giberellin (GA3) hormone is needed. GA3 functions to...

Lichen has been investigated for discovering new antibacterial agents. Although Indonesia is considered one of the megadiverse countries and a home to a diverse array of lichen species, research on lichens for medicinal properties remains scarce. In this study, an intensive semi-preparative High-Performance Liquid Chromatography successfully isolat...

Abstract The potential source of bioactive protein from pigmented rice seed was investigated by buffered extracting from nine varieties (Merah-SP, Gogo Niti-2, Merah Wangi, Super Manggis, Lamongan-1, Mota, Ketan Hitam-2, Beureum Taleus, and Aek Sibundong) and non-pigmented rice (IR-64) as a control. The potent contributor to free radical scavenging...

BACKGROUND: The mice are categorized as the nocturnal animal meaning that most of the activity conducted in dark environment. However, the treatment of mice as the object studied in the laboratory often experiences the lack attention especially the treat to light exposure to mice during the investigation period which potentially affect the result....

Melinjo (Gnetum gnemon L.) is a beneficial agroforestry plant that contains antioxidant compounds broadly studied as a natural source in pharmaceutical and nutraceutical uses. The antioxidant compounds benefit human health and plant to develop defense mechanisms under environmental stresses, such as drought stress. This study aims to evaluate the d...

Pigmented rice has hight antioxidant nature, and its properties contribute to the successful formulation of nutraceutical food products. Based on this background, this research was aimed at determining the physicochemical, functional and antioxidant properties of ten pigmented Indonesian rice varieties - Merah Saleman, Mota, Beureum Taleus, Anak Do...

The role of genetic diversity in crop germplasm is an important concept within genetic conservation. In this research, 43 accessions were analyzed at the agro-morphological and genetic levels. Clustering based on the agro-morphological resulted in four sub-groups. Analysis at the genetic level was conducted using 22 microsatellites, which revealed...

Pigmented rice (Oryza sativa L.) becomes interestingly important in agroindustry because it contains high phytochemical compounds that can be antioxidants and metal ion chelating. This study attempted to relate the content of phenols, flavonoids, anthocyanin, and bioactive phytochemicals activity from differently pigmented rice (red and black) vari...

Tomato was classified as a climacteric fruit with a fast ripening period andthen followed by the increase of fruit softening. The inhibition of fruit softening was needed to maintain the quality and shelf life of the fruit. One of the way to inhibit the tomatoes softening was calcium application. However, the best concentration and timing of calciu...

Coffee peel waste contains fibers including xylan, a polymer that constitutes hemicelluloses. Xylan can be extracted from coffee peel and used for xylooligosaccharide (XOS) production. XOS is an oligomer that has the potentials as a prebiotic. XOS production could be carried out by enzymatic hydrolysis using endo-β-1,4-d-xylanase. In this research,...

roteins hydrolyzed from melinjo seeds (Gnetum gnemon) at green (GM), yellow (YM) and red (RM) stages of maturity were studied for their effectiveness in antioxidant and antidiabetic activities. The seed protein extract was hydrolyzed using alcalase 2.4L, and the resulting hydrolysates with the highest degree of hydrolysis, protein profile, and the...

Preparation and characterization of a novel cocrystal of atorvastatin calcium with succinic acid coformer were successfully performed. This research aims to modify the crystalline form of atorvastatin calcium through cocrystallization with succinic acid coformer. The cocrystal was prepared by a solvent evaporation method and characterized by Powder...

Cinnamomum sintoc Bl. merupakan tanaman yang memiliki kandungan minyak atsiri di kulit batangnya yang digunakan untuk antioksidan, antihiperlipidemia dan lain-lain. Dari penelitian ini, senyawa metabolit sekunder dari daun sintok (kadar air 54,7%±0,69) diekstrak dengan metanol dan dianalisis dengan reagen spesifik. Daun sintok diekstraksi secara ma...

The majority of tissue culture and transformation studies in cassava (Manihot esculenta Crantz) focus on the modification of conditions in order to establish a better protocol. Although this is a standard approach for making progress in genetic transformation technology for a target plant variety, serious difficulty still remains due to the limited...

Green Deli Rose Apples (Syzygium samarangense) has several beneficial properties in terms of usability, cultivation, health, and economy. The intensive development of green deli rose apples plants is needed given increasing demand, needs, and has bright prospects. One of the efforts to develop green deli rose apples plants is through handling plant...

Desa Baratan, Kecamatan Patrang, Kabupaten Jember memiliki potensi tinggi dalam usaha produksi keripik singkong yang dikelola oleh Industri Rumah Tangga (IRT). Limbah padat berupa kulit singkong yang dihasilkan dari pengolahan singkong menjadi kripik singkong belum termanfaatkan dengan baik. Kulit singkong dapat digunakan sebagai bahan baku pakan t...

Aims: The objective of this research was to isolate caffeine-degrading bacteria from coffee pulp waste in Indonesia and characterize their caffeine degradation activity. Methodology and results: The caffeine-degrading bacteria were isolated from coffee pulp wastes of Coffea arabica and C. canephora. These isolates were selected based on their caffe...

Eighteen extracts of six medicinal plants of Meru Betiri National Park (Jember, Indonesia) were studied for their anti-diabetic potency as well as their antioxidant activities (DPPH, superoxide anion and hydroxyl radical scavenging). Total phenolic and flavonoid content were also determined. Total phenols and flavonoid content increased with polari...

Hypertension is ranked third on the cause of death in Indonesia for all ages (6.8 %). Protein isolate of melinjo seeds (Gnetum gnemon L.) hydrolyzed with free alcalase has been known to have antihypertensive activity. However, the use of free alcalase is uneconomical since it can only be used once, thus we studied the immobilized alcalase effectivi...

Melinjo is the family of Gnetaceae that contains high antioxidant compound withthe potentialsof being medicinal plant useful for human health. In plant, antioxidant functions as defense system against reactive oxygen species (ROS) highly accumulated by unfavorable environment such as drought stress. Ascorbate peroxidase (APX) is one of the antioxid...

Binary mixtures of pharmaceuticals significantly affect the physical and chemical properties of each component. The aim of this work was to explore the thermal behavior and solid state transformation of binary mixture of atorvastatin calcium and succinic acid. The thermodynamics of binary mixtures of atorvastatin calcium - succinic acid were determ...

The synthetic of antioxidant peptides from melinjo (Gnetum gnemon) seed protein isolated using sol-gel immobilized alcalase was studied, a commercial alcalase was physically entrapped in sol-gel matrices system. Characteristics of protein hydrolyzed were evaluated by the degree of hydrolysis obtained (DH) and the peptide profile by FPLC and SDS-PAG...

Purpose: To investigate the formation of atorvastatin calcium (AC) co-crystal to improve its solubility and dissolution rate. Method: Co-crystallization of AC in equimolar ratio with isonicotinamide (INA) was carried out by slow solvent evaporation method using methanol. The solid obtained was characterized by powder x-ray diffraction (PXRD), diffe...

The radical scavenging capacities and DNA damage protection of fermented melinjo (Gnetum gnemon) flour were investigated to determine its potential use as a nutraceutical ingredient. Fermented melinjo flour was prepared using a Lactobacillus fermentum starter culture. The fermented and unfermented melinjo flours were examined for antioxidant activi...

Study activities phenolic extract of bungur leaf as an antioxidant and inhibitor for the enzyme α-glucosidase was performed by spectrophotometric method. The extraction was carried out by three solvents with different polarity level in order to get three types of extracts namely hexane extract Bungur (HB), ethyl acetate extract Bungur (EAB), and th...

Kayu kuning has already used as traditional medicine to treat various disease. The aim of this study was to evaluate the in vitro antioxidant and antidiabetic activity of hexane, ethyl acetate, and methanol extract of Kayu kuning leaves. The antioxidant activities of the Kayu kuning extracts were measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH), s...

Invertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the key enzymes involved in sucrose metabolism in sugarcane plants. They are highly correlated with sucrose and reducing sugar contents during plant growth. The sugarcane plants have two kinds of invertases, namely neutral invertase (NI) and acid invertase (AI). They have different function...

Invertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the key enzymes involved in sucrose metabolism in sugarcane plants. They are highly correlated with sucrose and reducing sugar contents during plant growth. The sugarcane plants have two kinds of invertases, namely neutral invertase (NI) and acid invertase (AI). They have different function...

Cocrystal is one method to improve the physical-chemical properties of active pharmaceutical ingredient. Atorvastatin calcium is a cholesterol-lowering drug that has poorly soluble in water. The purpose of this study was to cocrystallization atorvastatin calcium with malonic acid as coformer. Cocrystal of atorvastatin calcium-malonic acid in equimo...

The sulfhydryl oxidase (SHOxase; EC. 1.8.3.2) which catalyzes the oxidation of sulfhydryl groups to disulfides was purified from Aspergillus niger using a combination of chromatographies. The influence of SHOxase on starch properties was tested by rheometer, farinographand scanning electron microscopy (SEM). Moreover, the gelatinization and retrogr...

The acylated antocyanins of jambolan fruit were synthesized by lipase-catalyzed transesterification reaction with cinnamic acid (intramolecular interaction). The native anthocyanins of jambolan (non-acylated form) were successfully converted to anthocyanin cinnamate (acylated form) by the enzymatic system. The products of synthesis were determined...

Protein was one of macromolecule in seed which played an important role in the process of germination. Storage protein in seed was hydrolyzed to amino acid which are then used in the translation process for a genetic message interpretating and protein building in pre-germination process. Pre-Germination was the first step of germination that have m...

Research on the development of botanical pesticides should be developed through new methods, such as by inhibiting the activity of digestive enzymes by secondary metabolites. The aim of this study was to identify some of potential plants as a source of tannin-protein complexes to inhibit the activity of - amylase. The study of identification of po...

In plants, transgenes with inverted repeats are used to induce efficient RNA silencing, which is also frequently induced by highly transcribed sense transgenes. RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which converts single-stranded (ss) RNA into double-stranded (ds) RNA. By contrast, it has b...

The protein from the seeds of melinjo ( Gnetum gnemon ) was purified using a precipitation method and ion exchange chromatographic techniques to identify the potent antioxidant and free radical scavenging activities. Two antioxidant protein fractions were isolated from G. gnemon seed with molecular weights of approximately 30 kDa (Gg-AOPI) and 12 k...

L-ascorbyl palmitate (AsA-Pal-Enz) was synthesized by using an immobilized lipase from Aspergillus niger. A comparison of antioxidative effects between L-ascorbic acid (AsA) and AsA-Pal-Enz was determined in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical–scavenging. The results indicate that the AsA-Pal-Enz was effective in preventing lipid...

Breadfruit starch samples were prepared after annealing at 45–60°C for 24 h and the relationship between the thermal properties and the retrogradation of starch was investigated using differential scanning calorimetry. The peak temperature (Tp) increased with increasing annealing temperature but enthalpy value was decreased with increasing annealin...

Invertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the key enzymes involved in sucrose metabolism in sugarcane plants. They are highly correlated with sucrose and reducing sugar con-tents during plant growth. The sugarcane plants have two kinds of invertases, namely neutral invertase (NI) and acid invertase (AI). They have different function...

Starch inclusion complexes with mono- and diacyl-sn-glycerophosphatidyl-choline (GPC) having various acyl (myristoyl, palmitoyl, stearoyl) chain lengths were studied. The physicochemical properties, such as the iodine affinities; crystallinity; water sorption and thermal properties of the defatted starch inclusion complexes were examined. Iodine af...

Complexes of starch and monoacyl-sn-glycerophosphatidylcholine (GPC) containing various acyl (myristoyl, palmitoyl, and stearoyl) chains were subjected to hydrolysis with glucoamylase (EC 3.2.1.3). The enzyme hydrolyzed approximately 40% of starch control and 20-28% of starch-GPC complexes. Among the GPCs examined, 1- and 2-monomyristoyl-sn-GPC sho...

Formation of amylose–glycerophosphatidylcholine (GPC) complexes having various monoacyl chain lengths, and their thermal properties and kinetic parameter of the complexes were studied by differential scanning calorimetry (DSC). Complexes were studied at a concentration of 10% (w/w) on a dry amylose. The melting temperature (Tm) of amylose–lipid com...

The influence of starch with 1- or 2-monoacyl-sn-glycerophosphatidylcholine (GPC) having various chain lengths of fatty acids on gelatinization and retrogradation of starch was studied by the measurement of starch-GPC complex formation, complexing index, and differential scanning calorimetry. The addition of GPC to the starch sample slightly increa...

The effects of phospholipids (PLs) transesterified with polyunsaturated fatty acids (PUFAs) with lipase (Aspergillus niger) on gelatinization and retrogradation of starch during storage were studied by differential scanning calorimetry (DSC). The resulting transesterified PLs were rich in PUFAs and linoleic acid, while the total percentage of PUFAs...

The effects of polyunsaturated phospholipids on changes in the thermal and physical properties of starch systems during mixing, baking and storage were studied. In the presence of phospholipids (PLs) or polyunsaturated fatty acid-containing PLs (PUFA-PLs), the viscoelastic properties of starch dough, such as stress, modulus of elasticity and viscos...

The effects of phospholipids (PLs) transesterified with polyunsaturated fatty acids (PUFAs) with lipase (Aspergillus niger) on gelatinization and retrogradation of starch during storage were studied by differential scanning calorimetry (DSC), The resulting transesterified PLs were rich in PUFAs and linoleic acid, while the total percentage of PUFAs...

The effects of purified lipase and α-amylase were tested for the retrogradation of starch in bread and their impact on some rheological properties. The combined addition of lipase and α-amylase increased significantly the loaf volume compared with the control, and the farinograph data showed that combined addition shortened the arrival time and sli...

The effects of purified lipase and α-amylase were tested for the retrogradation of starch in bread and their impact on some rheological properties. The combined addition of lipase and α-amylase increased significantly the loaf volume compared with the control, and the farinograph data showed that combined addition shortened the arrival time and sli...

Physicochemical characteristics of Gnetum gnemon (mlinjo) starch-lipids as influence of different extract of lipids on changes in lipids composition were studied. The mlinjo samples were extracted sequentially with hexane for surface lipid (SL) and hot water saturated butanol for internal lipid (IL). The total lipid content (dwb) comprising surface...